Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	94,724	G→T	intergenic (‑214/+88)	unknown ← / ← unknown	NADP‑specific glutamate dehydrogenase (EC 1.4.1.4)/Argininosuccinate lyase (EC 4.3.2.1)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	94,724	0	G	T	100.0%	177.4 / NA	62	intergenic (‑214/+88)	unknown/unknown	NADP‑specific glutamate dehydrogenase (EC 1.4.1.4)/Argininosuccinate lyase (EC 4.3.2.1)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (36/26);  total (36/26)

GGAAGGGACCGAACCAGACATAAGAAATCTCCTGTAAGATGAATGTTGTTGTTTGCATTTGAGTGAATTGTGTGTTGCTCAACTATGTTGTTAAGTCGGTTTTCAATATGCAACAGCCGGGGAGATGATTTCAAATGTTTTTCTAGAAAAATATTAGAATATTTTGTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTGGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCAGAGACAGAATATCGTACACATCGTCCGACACTTTTTCGCAGAATTGACGGAATTCCGG  >  1/94490‑94967                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                    ggaaggGACCGAACCAGACATAAGAAATCTCCTGTAAGATGAATGTTGTTGTTTGCATTTGAGTGAATTGTGTGTTGCTCAACTATGTTGTTAAGTCGGTTTTCAATATGCAACAGCCGGGGAGATGATTTCAAATGTTTTTCTAGAAAAATATTAGAATATTTTGTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGaaa                                                                                                                                                                                                                                     >  1:345372/1‑251 (MQ=255)           gAACCAGACATAAGAAATCTCCTGTAAGATGAATGTTGTTGTTTGCATTTGAGTGAATTGTGTGTTGCTCAACTATGTTGTTAAGTCGGTTTTCAATATGCAACAGCCGGGGAGATGATTTCAAATGTTTTTCTAGAAAAATATTAGAATATTTTGTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTaa                                                                                                                                                                                                                           >  1:75086/1‑251 (MQ=255)              ccAGACATAAGAAATCTCCTGTAAGATGAATGTTGTTGTTTGCATTTGAGTGAATTGTGTGTTGCTCAACTATGTTGTTAAGTCGGTTTTCAATATGCAACAGCCGGGGAGATGATTTCAAATGTTTTTCTAGAAAAATATTAGAATATTTTGTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTaaaaa                                                                                                                                                                                                                        >  1:863526/1‑251 (MQ=255)                  aCATAAGAAATCTCCTGTAAGATGAATGTTGTTGTTTGCATTTGAGTGAATTGTGTGTTGCTCAACTATGTTGTTAAGTCGGTTTTCAATATGCAACAGCCGGGGAGATGATTTCAAATGTTTTTCTAGAAAAATATTAGAATATTTTGTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAaaacaaa                                                                                                                                                                                                                    >  1:728030/1‑251 (MQ=255)                     tAAGAAATCTCCTGTAAGATGAATGTTGTTGTTTGCATTTGAGTGAATTGTGTGTTGCTCAACTATGTTGTTAAGTCGGTTTTCAATATGCAACAGCCGGGGAGATGATTTCAAATGTTTTTCTAGAAAAATATTAGAATATTTTGTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAacaaacaa                                                                                                                                                                                                                 >  1:107133/1‑251 (MQ=255)                          aaTCTCCTGTAAGATGAATGTTGTTGTTTGCATTTGAGTGAATTGTGTGTTGCTCAACTATGTTGTTAAGTCGGTTTTCAAGATGCAACAGCCGGGGAGATGATTTCAAATGTTTTTCTAGAAAAATATTAGAATATTTTGTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTg                                                                                                                                                                                                            <  1:440861/251‑1 (MQ=255)                               ccTGTAAGATGAATGTTGTTGTTTGCATTTGAGTGAATTGTGTGTTGCTCAACTATGTTGTTAAGTCGGTTTTCAATATGCAACAGCCGGGGAGATGATTTCAAATGTTTTTCTAGAAAAATATTAGAATATTTTGTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAaaac                                                                                                                                                                                                                       <  1:260128/235‑1 (MQ=255)                                         gAATGTTGTTGTTTGCATTTGAGTGAATTGTGTGTTGCTCAACTATGTTGTTAAGTCGGTTTTCAATATGCAACAGCCGGGGAGATGATTTCAAATGTTTTTCTAGAAAAATATTAGAATATTTTGTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTaaaa                                                                                                                                                                                                                         >  1:898435/1‑223 (MQ=255)                                                   gttTGCATTTGAGTGAATTGTGTGTTGCTCAACTATGTTGTTAAGTCGGTTTTCAATATGCAACAGCCGGGGAGATGATTTCAAATGTTTTTCTAGAAAAATATTAGAATATTTTGTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTg                                                                                                                                                                                                            <  1:340676/226‑1 (MQ=255)                                                              aGTGAATTGTGTGTTGCTCAACTATGTTGTTAAGTCGGTTTTCAATATGCAACAGCCGGGGAGATGATTTCAAATGTTTTTCTAGAAAAATATTAGAATATTTTGTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACtt                                                                                                                                                                        >  1:589622/1‑251 (MQ=255)                                                                    ttGTGTGTTGCTCAACTATGTTGTTAAGTCGGTTTTCAATATGCAACAGCCGGGGAGATGATTTCAAATGTTTTTCTAGAAAAATATTAGAATATTTTGTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTacgac                                                                                                                                                                  <  1:332943/251‑1 (MQ=255)                                                                           ttGCTCAACTATGTTGTTAAGTCGGTTTTCAATATGCAACAGCCGGGGAGATGATTTCAAATGTTTTTCTAGAAAAATATTAGAATATTTTGTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTGAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTa                                                                                                                                                           >  1:444786/1‑251 (MQ=255)                                                                              cTCAACTATGTTGTTAAGTCGGTTTTCAATATGCAACAGCCGGGGAGATGATTTCAAATGTTTTTCTAGAAAAATATTAGAATATTTTGTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTacg                                                                                                                                                         <  1:338297/250‑1 (MQ=255)                                                                                cAACTATGTTGTTAAGTCGGTTTTCAATATGCAACAGCCGGGGAGATGATTTCAAATGTTTTTCTAGAAAAATATTAGAATATTTTGTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTacgacg                                                                                                                                                      >  1:859840/1‑251 (MQ=255)                                                                                     aTGTTGTTAAGTCGGTTTTCAATATGCAACAGCCGGGGAGATGATTTCAAATGTTTTTCTAGAAAAATATTAGAATATTTTGTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAAt                                                                                                                                                 >  1:753013/1‑251 (MQ=255)                                                                                                 cGGTTTTCAATATGCAACAGCCGGGGAGATGATTTCAAATGTTTTTCTAGAAAAATATTAGAATATTTTGTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCg                                                                                                                                     <  1:593117/251‑1 (MQ=255)                                                                                                          atatGCAACAGCCGGGGAGATGATTTCAAATGTTTTTCTAGAAAAATATTAGAATATTTTGTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATg                                                                                                                            >  1:455926/1‑251 (MQ=255)                                                                                                             tGCAACAGCCGGGGAGATGATTTCAAATGTTTTTCTAGAAAAATATTAGAATATTTTGTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCt                                                                                                                         <  1:83966/251‑1 (MQ=255)                                                                                                                     ccGGGGAGATGATTTCAAATGTTTTTCTAGAAAAATATTAGAATATTTTGTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGC‑TTTGCGTCAGCAATGGCTTCGGc                                                                                                                    <  1:805537/247‑1 (MQ=255)                                                                                                                             atgatTTCAAATGTTTTTCTAGAAAAATATTAGAATATTTTGTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAAc                                                                                                          <  1:443089/250‑1 (MQ=255)                                                                                                                              tgatTTCAAATGTTTTTCTAGAAAAATATTAGAATATTTTGTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACgg                                                                                                        <  1:694309/251‑1 (MQ=255)                                                                                                                                          tttttCTAGAAAAATATTAGAATATTTTGTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGcc                                                                                            <  1:721021/251‑1 (MQ=255)                                                                                                                                                       atatTAGAATATTTTGTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTacgac                                                                                                                                                       <  1:569397/179‑1 (MQ=255)                                                                                                                                                       atatTAGAATATTTTGTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGt                                                                               >  1:397472/1‑251 (MQ=255)                                                                                                                                                        tatTAGAATATTTTGTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGtt                                                                              >  1:689631/1‑251 (MQ=255)                                                                                                                                                              aaTATTTTGTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTc                                                                          <  1:616450/249‑1 (MQ=255)                                                                                                                                                                    ttGTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAgg                                                                  <  1:426287/251‑1 (MQ=255)                                                                                                                                                                      gTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGAt                                                                >  1:162265/1‑251 (MQ=255)                                                                                                                                                                            ataGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTacg                                                                                                                                                         <  1:214619/156‑1 (MQ=255)                                                                                                                                                                                aaaTGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCAGAGAc                                                       <  1:95407/250‑1 (MQ=255)                                                                                                                                                                                aaaTGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGGGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGGCCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGACAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCAGAGAc                                                       <  1:371053/250‑1 (MQ=255)                                                                                                                                                                                    gTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGc                                                                                                                                                     <  1:582266/152‑1 (MQ=255)                                                                                                                                                                                    gTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGc                                                                                                                    >  1:700091/1‑185 (MQ=255)                                                                                                                                                                                     ttttAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCAGAGACAGAat                                                  <  1:762209/250‑1 (MQ=255)                                                                                                                                                                                     ttttAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCAGAGACAGAat                                                  <  1:247720/250‑1 (MQ=255)                                                                                                                                                                                          gATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCAGAGACAGAATATCGTa                                            >  1:8067/1‑251 (MQ=255)                                                                                                                                                                                          gATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCAGAGACAGAATATCGTa                                            >  1:872626/1‑251 (MQ=255)                                                                                                                                                                                           aTTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCAGAGACAGAATATCGTac                                           <  1:160891/251‑1 (MQ=255)                                                                                                                                                                                             tttATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCAGAGACAGAATATCGTacac                                         <  1:124502/251‑1 (MQ=255)                                                                                                                                                                                              ttATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCAGAGACAGAat                                                  >  1:397975/1‑241 (MQ=255)                                                                                                                                                                                                      taaAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCAGAGACAGAATATCGTACACATCGTCCGa                                >  1:868436/1‑251 (MQ=255)                                                                                                                                                                                                             atatTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCAGAGACAGAATATCGTACACATCGTCCGACACtttt                         >  1:394930/1‑251 (MQ=255)                                                                                                                                                                                                             atatTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCAGAGACAGAATATCGTACACATCGTCCGACACtttt                         >  1:709671/1‑251 (MQ=255)                                                                                                                                                                                                              tatTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCagag                                                         <  1:766580/218‑1 (MQ=255)                                                                                                                                                                                                              tatTTATGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCAGAGACAGAATATCGTACACATCGTCCGACACttttt                        >  1:655681/1‑251 (MQ=255)                                                                                                                                                                                                                   aTGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCAc                                                                                                                                                                          >  1:340470/1‑100 (MQ=255)                                                                                                                                                                                                                   aTGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTacgac                                                                                                                                                                  >  1:823962/1‑108 (MQ=255)                                                                                                                                                                                                                   aTGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCAGAGACAGAATATCGTACACATCGTCCGACACTTTTTCGCAg                   >  1:650436/1‑251 (MQ=255)                                                                                                                                                                                                                   aTGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCAGAGACAGAATATCGTACACATCGTCCGACACTTTTTCGCAg                   >  1:12831/1‑251 (MQ=255)                                                                                                                                                                                                                   aTGTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCAGAGACAGAATATCGTACACATCGTCCGACACTTTTTCGCAg                   >  1:386431/1‑251 (MQ=255)                                                                                                                                                                                                                     gTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAAcc                                                                                                 >  1:226358/1‑171 (MQ=255)                                                                                                                                                                                                                     gTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCAGAGACAGAATATCGTACACATCGTCCGacac                             >  1:365640/1‑239 (MQ=255)                                                                                                                                                                                                                     gTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCAGAGACAGAATATCGTACACATCGTCCGACACTTTTTCGCAGaa                 >  1:892864/1‑251 (MQ=255)                                                                                                                                                                                                                     gTTAAACGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCAGAGACAGAATATCGTACACATCGTCCGACACTTTTTCGCAGaa                 >  1:262377/1‑251 (MQ=255)                                                                                                                                                                                                                          aCGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCAGAGACAGAATATCGTac                                           <  1:539136/220‑1 (MQ=255)                                                                                                                                                                                                                           cGCTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCAGAGACAGAATATCGTACACATCGTCCGACACTTTTTCGCAGAATTGACg           <  1:380018/251‑1 (MQ=255)                                                                                                                                                                                                                             cTGTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCAGAGACAGAatat                                                <  1:145299/212‑1 (MQ=255)                                                                                                                                                                                                                               gTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCag                                                           >  1:752828/1‑199 (MQ=255)                                                                                                                                                                                                                               gTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCAGAGACAGAATATCGTac                                           >  1:11326/1‑215 (MQ=255)                                                                                                                                                                                                                               gTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCAGAGACAGAATATCGTac                                           >  1:72972/1‑215 (MQ=255)                                                                                                                                                                                                                               gTTTAGTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCAGAGACAGAATATCGTACACATCGTCCGACACTTTTTCGCAGAATTGACGGAAt       >  1:145343/1‑251 (MQ=255)                                                                                                                                                                                                                                    gTTTTTTTGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCAGAGACAGAATATCGTACACATCGTCCGACACTTTTTCGCAGAATTGACGGAATTCCgg  >  1:543786/1‑251 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                    GGAAGGGACCGAACCAGACATAAGAAATCTCCTGTAAGATGAATGTTGTTGTTTGCATTTGAGTGAATTGTGTGTTGCTCAACTATGTTGTTAAGTCGGTTTTCAATATGCAACAGCCGGGGAGATGATTTCAAATGTTTTTCTAGAAAAATATTAGAATATTTTGTTTTTATAGAAATGTTTTAGATTTTATCTAATAAAAAAATATTTATGTTAAACGCTGTTTAGTTTTTTGGGTCGCGTAAGTGAAATCGGTGCTAAAAACAAACAAAAGTGCGGTCAGAAAAAATGATGTTTTTTATGACCGCACTTTACGACGTATTTACGACGCAAATCGGGCTTTTGCGTCAGCAATGGCTTCGGCAATGCGCAACGGTGAAACCCCGCCTTTGGCGGCACGTTTGTCTAAACAGGATTGCAGAGACAGAATATCGTACACATCGTCCGACACTTTTTCGCAGAATTGACGGAATTCCGG  >  1/94490‑94967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑