Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	874,251	T→C	P150P (CCT→CCC)	unknown →	CRISPR‑associated RecB family exonuclease Cas4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	874,251	0	T	C	100.0%	195.0 / NA	69	P150P (CCT→CCC)	unknown	CRISPR‑associated RecB family exonuclease Cas4
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/38);  total (31/38)

ACCGTTCACGTTTCGGCGGAGAAACTGGGCATCAGCGGCGTGCTGGATTTGGTGGAGGTCGAGGCGAAAACAGGTCGTCTGAAACCCGTGGAATATAAACGCGGCAAGCCCAAACTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCTGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGTCGGCGGGGTATGTAGATGAATTATTTGAGTATCTTGAAATAAGGAAAAAATATGAAAATTAAAACAATTGAAATTAAAA  >  1/874021‑874496                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      aCCGTTCACGTTTCGGCGGAGAAACTGGGCATCAGCGGCGTGCTGGATTTGGTGGAGGTCGAGGCGAAAACAGGTCGTCTGAAACCCGTGGAATATAAACGCGGCAAGCCCAAACTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAgacgac                                                                                                                                                                                                                                     >  1:631723/1‑249 (MQ=255)        aCGTTTCGGCGGAGAAACTGGGCATCAGCGGCGTGCTGGATTTGGTGGAGGTCGAGGCGAAAACAGGTCGTCTGAAACCCGTGGAATATAAACGCGGCAAGCCCAAACTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTccgcacc                                                                                                                                                                                                                            <  1:641670/251‑1 (MQ=255)             tCGGCGGAGAAACTGGGCATCAGCGGCGTGCTGGATTTGGTGGAGGTCGAGGCGAAAACAGGTCGTCTGAAACCCGTGGAATATAAACGCGGCAAGCCCAAACTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTccgcacccgca                                                                                                                                                                                                                        >  1:98201/1‑250 (MQ=255)                       aaCTGGGCATCAGCGGCGTGCTGGATTTGGTGGAGGTCGAGGCGAAAACAGGTCGTCTGAAACCCGTGGAATATAAACGCGGCAAGCCCAAACTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGccac                                                                                                                                                                                                              >  1:486474/1‑250 (MQ=255)                          tGGGCATCAGCGGCGTGCTGGATTTGGTGGAGGTCGAGGCGAAAACAGGTCGTCTGAAACCCGTGGAATATAAACGCGGCAAGCCCAAACTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATc                                                                                                                                                                                                          <  1:202842/251‑1 (MQ=255)                           gggCATCAGCGGCGTGCTGGATTTGGTGGAGGTCGAGGCGAAAACAGGTCGTCTGAAACCCGTGGAATATAAACGCGGCAAGCCCAAACTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATcg                                                                                                                                                                                                         >  1:595092/1‑251 (MQ=255)                                         tGCTGGATTTGGTGGAGGTCGAGGCGAAAACAGGTCGTCTGAAACCCGTGGAATATAAACGCGGCAAGCCCAAACTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGa                                                                                                                                                                                           <  1:764809/251‑1 (MQ=255)                                              gATTTGGTGGAGGTCGAGGCGAAAACAGGTCGTCTGAAACCCGTGGAATATAAACGCGGCAAGCCCAAACTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTAc                                                                                                                                                                                       <  1:776089/250‑1 (MQ=255)                                               aTTTGGTGGAGGTCGAGGCGAAAACAGGTCGTCTGAAACCCGTGGAATATAAACGCGGCAAGCCCAAACTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTccgcacccg                                                                                                                                                                                                                          >  1:347506/1‑214 (MQ=255)                                               aTTTGGTGGAGGTCGAGGCGAAAACAGGTCGTCTGAAACCCGTGGAATATAAACGCGGCAAGCCCAAACTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTa                                                                                                                                                                                        >  1:177959/1‑248 (MQ=255)                                               aTTTGGTGGAGGTCGAGGCGAAAACAGGTCGTCTGAAACCCGTGGAATATAAACGCGGCAAGCCCAAACTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTa                                                                                                                                                                                        >  1:760182/1‑248 (MQ=255)                                               aTTTGGTGGAGGTCGAGGCGAAAACAGGTCGTCTGAAACCCGTGGAATATAAACGCGGCAAGCCCAAACTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTa                                                                                                                                                                                        >  1:297995/1‑248 (MQ=255)                                                  tggtggAGGTCGAGGCGAAAACAGGTCGTCTGAAACCCGTGGAATATAAACGCGGCAAGCCCAAACTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGaa                                                                                                                                                                                  <  1:263647/251‑1 (MQ=255)                                                    gtggAGGTCGAGGCGAAAACAGGTCGTCTGAAACCCGTGGAATATAAACGCGGCAAGCCCAAACTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGccac                                                                                                                                                                                                              <  1:207351/221‑1 (MQ=255)                                                                         ggTCGTCTGAAACCCGTGGAATATAAACGCGGCAAGCCCAAACTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGa                                                                                                                                                           >  1:241505/1‑251 (MQ=255)                                                                          gtcgtcTGAAACCCGTGAAATATAAACGCGGCAAGCCCAAACTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCAcc                                                                                                                                                              <  1:276408/247‑1 (MQ=255)                                                                              tcTGAAACCCGTGGAATATAAACGCGGCAAGCCCAAACTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTgg                                                                                                                                                                                    <  1:369784/221‑1 (MQ=255)                                                                                tGAAACCCGTGGAATATAAACGCGGCAAGCCCAAACTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGc                                                                                                                                                    <  1:157692/251‑1 (MQ=255)                                                                                           gAATATAAACGCGGCAAGCCCAAACTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGccgtccgt                                                                                                                                                                                              >  1:138802/1‑198 (MQ=255)                                                                                             atatAAACGCGGCAAGCCCAAACTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCCCGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGc                                                                                                                                                    >  1:435912/1‑238 (MQ=255)                                                                                              tataAACGCGGCAAGCCCAAACTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGc                                                                                                                                      <  1:703134/251‑1 (MQ=255)                                                                                                            gCCCAAACTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTg                                                                                                                                                                                     >  1:201032/1‑190 (MQ=255)                                                                                                            gCCCAAACTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTc                                                                                                                                >  1:352174/1‑243 (MQ=255)                                                                                                            gCCCAAACTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCtggtgg                                                                                                                        >  1:286762/1‑251 (MQ=255)                                                                                                             cccAAACTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGcc                                                                                                                                                                 >  1:260100/1‑209 (MQ=255)                                                                                                              ccAAACTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGgag                                                                                                                      <  1:332760/251‑1 (MQ=255)                                                                                                               cAAACTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGgaga                                                                                                                     <  1:803167/251‑1 (MQ=255)                                                                                                                 aaCTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGAtt                                                                                                                   >  1:889584/1‑251 (MQ=255)                                                                                                                     tGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGcc                                                                                                               <  1:895729/251‑1 (MQ=255)                                                                                                                               gACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCAtt                                                                                                     >  1:777963/1‑251 (MQ=255)                                                                                                                                     aTCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGc                                                                                                                                      <  1:213692/212‑1 (MQ=255)                                                                                                                                           ttttGCGCCCAAGGTCTGTGTTTGAAGGAAATGACGGGGCAAGCCGTCTTTGGGGGCGCGCTGTGGTATATGCAACCCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCTCCCGACTACGGCAAACGCTGCGAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGaa                                                                                            <  1:201320/247‑1 (MQ=255)                                                                                                                                                cgcCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAACCCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGgag                                                                                                                      <  1:364880/217‑1 (MQ=255)                                                                                                                                                  cccAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGccac                                                                                                                                                                                                              <  1:757792/127‑1 (MQ=255)                                                                                                                                                  cccAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAgg                                                                                  >  1:583611/1‑251 (MQ=255)                                                                                                                                                  cccAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAgg                                                                                  <  1:526505/251‑1 (MQ=255)                                                                                                                                                   ccAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGt                                                                                 <  1:887772/251‑1 (MQ=255)                                                                                                                                                         gCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGAt                                                                                     >  1:417361/1‑241 (MQ=255)                                                                                                                                                            gtgtTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTccgcacc                                                                                                                                                                                                                            <  1:408909/103‑1 (MQ=255)                                                                                                                                                                ttGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGTCGGCGGGGTATGt                                                                    <  1:421725/251‑1 (MQ=255)                                                                                                                                                                ttGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGTCGGCGGGGTATGt                                                                    <  1:640187/251‑1 (MQ=255)                                                                                                                                                                    aggaAATGCCGGGGCAACCCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGTCGGCGGGGTATGTAGa                                                                 <  1:689000/250‑1 (MQ=255)                                                                                                                                                                    aggaAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGTCGGCGGGGTATGTAGa                                                                 <  1:498092/250‑1 (MQ=255)                                                                                                                                                                    aggaAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGTCGGCGGGGTATGTAGa                                                                 <  1:602887/250‑1 (MQ=255)                                                                                                                                                                    aggaAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGTCGGCGGGGTATGTAGa                                                                 <  1:667055/250‑1 (MQ=255)                                                                                                                                                                      gaAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGTCGGCGGGGTATGTAGATg                                                               <  1:396905/250‑1 (MQ=255)                                                                                                                                                                          tGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGTCGGCGGGGTATGTAGATGAatta                                                          <  1:364358/251‑1 (MQ=255)                                                                                                                                                                               gggCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGTCGGCGGGGTATGTAGATGAATTATTTGa                                                     >  1:235261/1‑251 (MQ=255)                                                                                                                                                                                ggCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAAccc                                                                                                                                                                     >  1:294429/1‑138 (MQ=255)                                                                                                                                                                                  cAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGTCGGCGGGGTATGTAGATGAATTATTTGAGTa                                                  <  1:151087/251‑1 (MQ=255)                                                                                                                                                                                      ccGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGTCGGCGGGGTATGTAGATGAATTATTTg                                                      <  1:41658/243‑1 (MQ=255)                                                                                                                                                                                       cGTCCTTGAGGGCGCGCTGTGGTATAGGTAACCCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGGCGGCATTGTTGGGGAAACGGGATAGGTCGGCGGGGTATGTAGATGAATTATTTGAGTATCTTg                                             <  1:449624/251‑1 (MQ=255)                                                                                                                                                                                          ccTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGTCGGCGGGGTATGTAGATGAATTATTTGAGTATCTTGaaa                                          <  1:461679/251‑1 (MQ=255)                                                                                                                                                                                           cTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGTCGGCGGGGTATGTAGATGAATTATTTGAGTATCTTGAAAt                                         <  1:63530/251‑1 (MQ=255)                                                                                                                                                                                                       cTGTGGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGTCGGCGGGGGATGTa                                                                   >  1:20659/1‑213 (MQ=255)                                                                                                                                                                                                         gtgGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGTCGGCGGGGTATGTAGa                                                                 >  1:236342/1‑213 (MQ=255)                                                                                                                                                                                                         gtgGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGTCGGCGGGGTATGTAGa                                                                 <  1:738982/213‑1 (MQ=255)                                                                                                                                                                                                         gtgGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGTCGGCGGGGTATGTAGATg                                                               >  1:683784/1‑215 (MQ=255)                                                                                                                                                                                                         gtgGTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGTCGGCGGGGTATGTAGATGAATTATTTg                                                      >  1:820839/1‑224 (MQ=255)                                                                                                                                                                                                           ggTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTTCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGTCGGCGGGGTATGt                                                                    >  1:54547/1‑208 (MQ=255)                                                                                                                                                                                                           ggTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGTCGGCGGGGTATGTAGa                                                                 <  1:530999/211‑1 (MQ=255)                                                                                                                                                                                                           ggTATATGAAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTccgcaccc                                                                                                                                                                                                                           <  1:513439/57‑1 (MQ=255)                                                                                                                                                                                                            gTATATGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGTCGGCGGGGTATGTAGATg                                                               >  1:731540/1‑212 (MQ=255)                                                                                                                                                                                                              atatGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGTCGGCGGGGTATGTAGATGAATTATTTGAGTATCTTGAAATAAGGAAAAAATATGAAAAt                      >  1:312457/1‑251 (MQ=255)                                                                                                                                                                                                                atGCAAACCCGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGTcggcgg                                                                           <  1:573883/196‑1 (MQ=255)                                                                                                                                                                                                                        ccGCCACCGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGTCGGCGGGGTATGTAGATGAATTATTTGAGTATCTTGAAATAAGGAAAAAATATGAAAATTAAAACAAtt            >  1:141706/1‑251 (MQ=255)                                                                                                                                                                                                                               cGTGTCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGTCGGCGGGGTATGTAGa                                                                 <  1:361412/191‑1 (MQ=255)                                                                                                                                                                                                                                  gtCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGTCGGCGGGGTATGTAGATGAATTATTTGAGTATCTTGAAATAAGGAAAAAATATGAAAATTAAAACAATTGAAATTaaaa  >  1:674077/1‑251 (MQ=255)                                                                                                                                                                                                                                  gtCCCCGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGTCGGCGGGGTATGTAGATGAATTATTTGAGTATCTTGAAATAAGGAAAAAATATGAAAATTAAAACAATTGAAATTaaaa  >  1:620638/1‑251 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      ACCGTTCACGTTTCGGCGGAGAAACTGGGCATCAGCGGCGTGCTGGATTTGGTGGAGGTCGAGGCGAAAACAGGTCGTCTGAAACCCGTGGAATATAAACGCGGCAAGCCCAAACTTGAGCCTATGGACGAAATCCAGCTTTGCGCCCAAGGGCTGTGTTTGGAGGAAATGACGGGGCAAACCGTCCTTGAGGGCGCGCTGTGGTATATGCAAACCCGCCACCGTGTCCCTGTCGTCTTTTCAGACGACCTCCGCACCCGCACGCTAGCCACCATCGCCGCCGTCCGTGGATTACTGGAAAGCGGACAAACCCCGCCACCCGACTACGGCAAACGCTGCAAGGCCTGCTCGCTGGTGGAGATTTGCCAGCCGGCATTGTTGGGGAAACGGGATAGGTCGGCGGGGTATGTAGATGAATTATTTGAGTATCTTGAAATAAGGAAAAAATATGAAAATTAAAACAATTGAAATTAAAA  >  1/874021‑874496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑