| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 872,414 | T→G | A325A (GCT→GCG) | unknown → | CRISPR‑associated helicase Cas3 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 872,414 | 0 | T | G | 98.7% | 212.1 / ‑4.4 | 78 | A325A (GCT→GCG) | unknown | CRISPR‑associated helicase Cas3 |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (44/33); total (44/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.78e-01 | |||||||||||
TAAGCATTCAGTGTTAAATCAAGAGCGGAGTGCCATTTTAGCGCAATGTTTTTCTGCCGCAGAATCCGACCGCACTTTATTTTCTTTAACTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCTGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAGAACCAGCCGCTGCCGCAAGATTCACAATATCGCAGACAGCGTGATTATTTTGGATGAAGTCCAACAGCTTCCGCGCGATTTTCAAAAACCGATTACCGATATGA > 1/872168‑872655 | tAAGCATTCAGTGTTAAATCAAGAGCGGAGTGCCATTTTAGCGCAATGTTTTTCTGCCGCAGAATCCGACCGCACTTTATTTTCTTTAACTGTGCTGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGAtgcggtgc < 1:650953/251‑1 (MQ=255) aGCATTCAGTGTTAAATCAAGAGCGGAGTGCCATTTTAGCGCAATGTTTTTCTGCCGCAGAATCCGACCGCACTTTATTTTCTTTAACTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGAtgcggtg > 1:591695/1‑248 (MQ=255) gCATTCAGTGTTAAATCAAGAGCGGAGTGCCATTTTAGCGCAATGTTTTTCTGCCGCAGAATCCGACCGCACTTTATTTTCTTTAACTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTgg > 1:866827/1‑251 (MQ=255) aTTCAGTGTTAAATCAAGAGCGGAGTGCCATTTTAGCGCAATGTTTTTCTGCCGCAGAATCCGACCGCACTTTATTTTCTTTAACTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAg < 1:342450/251‑1 (MQ=255) gtgtTAAATCAAGAGCGGAGTGCCATTTTAGCGCAATGTTTTTCTGCCGCAGAATCCGACCGCACTTTATTTTCTTTAACTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAg > 1:898697/1‑246 (MQ=255) gtgtTAAATCAAGAGCGGAGTGCCATTTTAGCGCAATGTTTTTCTGCCGCAGAATCCGACCGCACTTTATTTTCTTTAACTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATca > 1:379978/1‑251 (MQ=255) gtgtTAAATCAAGAGCGGAGTGCCATTTTAGCGCAATGTTTTTCTGCCGCAGAATCCGACCGCACTTTATTTTCTTTAACTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATca > 1:85376/1‑251 (MQ=255) gtgtTAAATCAAGAGCGGAGTGCCATTTTAGCGCAATGTTTTTCTGCCGCAGAATCCGACCGCACTTTATTTTCTTTAACTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATca > 1:257415/1‑251 (MQ=255) tgtTAAATCAAGAGCGGAGTGCCATTTTAGCGCAATGTTTTTCTGCCGCAGAATCCGACCGCACTTTATTTTCTTTAACTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATcac < 1:348878/251‑1 (MQ=255) tAAATCAAGAGCGGAGTGCCATTTTAGCGCAATGTTTTTCTGCCGCAGAATCCGACCGCACTTTATTTTCTTTAACTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGc < 1:590372/251‑1 (MQ=255) aaaTCAAGGGCGGAGTGCCATTTTAGCGCAATGTTTTTCTGCCGCAGAATCCGACCGCACTTTATTTTCTTT‑ACTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCaa < 1:415629/251‑1 (MQ=255) aTCAAGAGCGGAGTGCCATTTTAGCGCAATGTTTTTCTGCCGCAGAATCCGACCGCACTTTATTTTCTTTAACTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAAt > 1:31754/1‑251 (MQ=255) cGGAGTGCCATTTTAGCGCAATGTTTTTCTGCCGCAGAATCCGACCGCACTTTATTTTCTTTAACTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAg < 1:847397/250‑1 (MQ=255) aTGTTTTTCTGCCGCAGAATCCGACCGCACTTTATTTTCTTTAACTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAg < 1:581125/211‑1 (MQ=255) aTGTTTTTCTGCCGCAGAATCCGACCGCACTTTATTTTCTTTAACTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAg > 1:618510/1‑211 (MQ=255) gTTTTTCTGCCGCAGAATCCGACCGCACTTTATTTTCTTTAACTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGc > 1:258974/1‑250 (MQ=255) gTTTTTCTGCCGCAGAATCCGACCGCACTTTATTTTCTTTAACTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGc > 1:751243/1‑250 (MQ=255) gCCGCAGAATCCGACCGCACTTTATTTTCTTTAACTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGAtgcggtgc > 1:376733/1‑196 (MQ=255) gAATCCGACCGCACTTTATTTTCTTTAACTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTtg > 1:582174/1‑250 (MQ=255) aCCGCACTTTATTTTCTTTAACTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGaa < 1:79238/251‑1 (MQ=255) gCACTTTATTTTCTTTAACTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGa < 1:321879/211‑1 (MQ=255) cTTTATTTTCTTTAACTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAg < 1:514653/182‑1 (MQ=255) cTTTATTTTCTTTAACTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTgg > 1:20463/1‑251 (MQ=255) aTTTTCTTTAACTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATg < 1:421956/251‑1 (MQ=255) ttCTTTAACTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGa > 1:837352/1‑246 (MQ=255) ttCTTTAACTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATgcg > 1:121265/1‑250 (MQ=255) ttCTTTAACTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATgcg > 1:440386/1‑250 (MQ=255) ttCTTTAACTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATgcg > 1:254986/1‑250 (MQ=255) aCTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGAt < 1:482308/251‑1 (MQ=255) gtgCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAAc > 1:790701/1‑203 (MQ=255) gtgCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAAc > 1:541654/1‑203 (MQ=255) gtgCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGt > 1:702318/1‑251 (MQ=255) gtgCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGt > 1:513885/1‑251 (MQ=255) gCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGa > 1:501068/1‑234 (MQ=255) ccGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTtac < 1:753707/251‑1 (MQ=255) gACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTg < 1:309238/245‑1 (MQ=255) gtggtgGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCaa < 1:873549/250‑1 (MQ=255) gtggtgGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCaa < 1:398211/250‑1 (MQ=255) aaaCCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAgttg < 1:575444/251‑1 (MQ=255) ttGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGa > 1:482716/1‑251 (MQ=255) aGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTAcc < 1:800234/228‑1 (MQ=255) gCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAg < 1:590936/123‑1 (MQ=255) gCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAg > 1:451283/1‑251 (MQ=255) cTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGtt < 1:19757/209‑1 (MQ=255) cTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAGa > 1:196132/1‑251 (MQ=255) cTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAg < 1:394809/110‑1 (MQ=255) cTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAGAACCAGCCGCTg > 1:484693/1‑250 (MQ=255) gTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAAc > 1:429142/1‑141 (MQ=255) gATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAGAACCAGCCGCTGCCGCAAGATTCACAATATCGCAg > 1:876038/1‑251 (MQ=255) aTTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAGAACCAGCCGCTGCCGCAAGATTCACAATATCGCAg < 1:751720/250‑1 (MQ=255) ttATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAGAACCAGCCGCTGCCGCAAGATTCACAATATCGCAGAc < 1:394121/251‑1 (MQ=255) aTCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCC‑TGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAGAACCAGCCGCTGCCGCAAGATTCACAATATCGCAGACAg > 1:63277/1‑250 (MQ=255) aCGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAGAAc < 1:76938/211‑1 (MQ=255) cATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAGAACCAGCCGCTGCCGCAAGATTCACAATATCGCAGACAGCGTGatta < 1:191686/251‑1 (MQ=255) cccTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGa > 1:228343/1‑182 (MQ=255) cccTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAGAACCAGCCGCTGCCGCAAGATTCACAATATCGCAGAc > 1:810963/1‑237 (MQ=255) cccTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAGAACCAGCCGCTGCCGCAAGATTCACAATATCGCAGAc < 1:647498/237‑1 (MQ=255) cccTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAGAACCAGCCGCTGCCGCAAGATTCACAATATCGCAGAc > 1:398158/1‑237 (MQ=255) cccTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAGAACCAGCCGCTGCCGCAAGATTCACAATATCGCAGACAGCGTGATTAtttt > 1:425134/1‑251 (MQ=255) cccTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAGAACCAGCCGCTGCCGCAAGATTCACAATATCGCAGACAGCGTGATTAtttt > 1:558859/1‑251 (MQ=255) cccTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCAGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAGAACCAGCCGCTGCCGCAAGATTCACAATATCGCAGACAGCGTGATTAtttt > 1:365642/1‑251 (MQ=255) cccTTTACCAGCATTATTGAACAAAACGCCGATTTTTCCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAGAACCAGCCGCTGCCGCAAGATTCACAATATCGCAGAc > 1:271071/1‑237 (MQ=255) ccTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAgttg > 1:785614/1‑176 (MQ=255) tttACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAGAACCAGCCGCTGCCGCAAGATTCACAATATCGCAg > 1:713842/1‑232 (MQ=255) ttACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAGAACCAGCCGCTGCCGCAAGATTCACAATATCGCAGACAGCGTGATTATTTTGGAt < 1:566308/251‑1 (MQ=255) gCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGaa < 1:333152/98‑1 (MQ=255) gCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGG‑AAACCGGGATGCGCCGTTGATTGTTACTAcc > 1:226900/1‑154 (MQ=255) ttattGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCTGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCa < 1:602285/153‑1 (MQ=255) ttattGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAAtgtg > 1:351344/1‑158 (MQ=255) ttattGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAGAACCAGCCGCTGCCGCAAGATTCACAATATCGCAGAc < 1:500725/224‑1 (MQ=255) tattGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTTCAGTTGTTTGAAAGCCTGTTTGCGGCAAGAACCAGCCGCTGCCGCAAGATTCACAATATCGCAGACAGCGTGATTATTTTGGATGAAGTCCAAc < 1:310287/251‑1 (MQ=255) gAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACTAAtg < 1:364291/151‑1 (MQ=255) gAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAGAACCAGCCGCTGCCGCAAGATTCACAATATCGCAGACAGCGTGATTATTTTGGATGAAGTCCAACAg > 1:167357/1‑249 (MQ=255) tttCCGTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAGGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAGAACCAGCCGCTGCCGCAAGATTCACAATATCGCAGACAGCGTGATTATTTTGGATGAAGTCCAACAGCTTCCGCGCGATTTTCaaa < 1:223436/251‑1 (MQ=255) gTAAAGCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAGAACCAGCCGCTGCCGCAAGATTCACAATATCGCAGACAGCGTGATTATTTTGGATGAAGTCCAACAGCTTCCGCGCGATTTTCAAAAACCg > 1:741784/1‑251 (MQ=255) gCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAGAACCAGCCGCTGCCGCAAGATTCACAATATCGCAGACAGCGTGATTATTTTGGATGAAGTCCAACAGCTTCcgcg > 1:328137/1‑230 (MQ=255) gCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAGAACCAGCCGCTGCCGCAAGATTCACAATATCGCAGACAGCGTGATTATTTTGGATGAAGTCCAACAGCTTCCGCGCGATTTTCAAAAACCGATTa > 1:749597/1‑250 (MQ=255) gCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAGAACCAGCCGCTGCCGCAAGATTCACAATATCGCAGACAGCGTGATTATTTTGGATGAAGTCCAACAGCTTCCGCGCGATTTTCAAAAACCGATTa > 1:361087/1‑250 (MQ=255) gCATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAGAACCAGCCGCTGCCGCAAGATTCACAATATCGCAGACAGCGTGATTATTTTGGATGAAGTCCAACAGCTTCCGCGCGATTTTCAAAAACCGATTa > 1:94836/1‑250 (MQ=255) cATTGGGCGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAGAACCAGCCGCTGCCGCAAGATTCACAATATCGCAGACAGCGTGATTATTTTGGATGAAGTCCAACAGCTTCCGCGCGATTTTCAAAAACCGATTAcc < 1:561534/251‑1 (MQ=255) cGATGATGCGGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAGAACCAGCCGCTGCCGCAAGATTCACAATATCGCAGACAGCGTGATTATTTTGGATGAAGTCCAACAGCTTCCGCGCGATTTTCAAAAACCGATTACCGATatga < 1:737575/251‑1 (MQ=255) | TAAGCATTCAGTGTTAAATCAAGAGCGGAGTGCCATTTTAGCGCAATGTTTTTCTGCCGCAGAATCCGACCGCACTTTATTTTCTTTAACTGTGCCGACAGGTGGTGGCAAAACCTTGGCGAGCTTGGGGTTTGCTTTAAAACACGCTTTGAAGTTCGGTAAAAAACGGATTATCTACGCCATTCCCTTTACCAGCATTATTGAACAAAACGCCGATTTTTTCCGTAAAGCATTGGGCGATGATGCTGTGCTGGAGCATCACAGCAATTTGGAAGTCAAGGAAGACAAGGAAACTGCCAAAACGCGCCTTGCTGCGGAAAACTGGGATGCGCCGTTGATTGTTACTACCAATGTGCAGTTGTTTGAAAGCCTGTTTGCGGCAAGAACCAGCCGCTGCCGCAAGATTCACAATATCGCAGACAGCGTGATTATTTTGGATGAAGTCCAACAGCTTCCGCGCGATTTTCAAAAACCGATTACCGATATGA > 1/872168‑872655 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |