Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	858,223	T→C	L182P (CTG→CCG)	unknown →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	858,223	0	T	C	100.0%	43.5 / NA	21	L182P (CTG→CCG)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/3);  total (18/3)

TCATCTTCTCAAAACTAAAATAATCAAAGTAATCGAAACATTTGACGGTTATAAGGATTTAAACTGCTTAGCCAGTTGGCTATTATTTAGCGGTCAGCAAGTCGCAACACTTAGTGATCTTTATCATAAAGATTTTTGGAATTGTATTAGAAAATCAGATTATCCAAATGCCCATGGTTATTTGGATGGTATAGAGATTACCAATGAATCATTCCACACGTTACTTCTGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAGGATAAAGTCAATAATTGGCAGGCATTTGATGGTGCACAAAGAATTA  >  1/857996‑858465                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   tcatcTTCTCAAAACTAAAATAATCAAAGTAATCGAAACATTTGACGGTTATAAGGATTTAAACTGCTTAGCCAGTTGGCTATTATTTAGCGGTCAGCAAGTCGCAACACTTAGTGATCTTTATCATAAAGATTTTTGGAATTGTATTAGAAAATCAGATTATCCAAATGCCCATGGTTATTTGGATGGTATAGAGATTACCAATGAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAggg                                                                                                                                                                                                                             >  1:258403/1‑251 (MQ=255)            aaaCTAAAATAATCAAAGTAATCGAAACATTTGACGGTTATAAGGATTTAAACTGCTTAGCCAGTTGGCTATTATTTAGCGGTCAGCAAGTCGCAACACTTAGTGATCTTTATCATAAAGATTTTTGGAATTGTATTAGAAAATCAGATTATCCAAATGCCCATGGTTATTTGGATGGTATAGAGATTACCAATGAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTtgtg                                                                                                                                                                                                                  <  1:396270/251‑1 (MQ=255)            aaaCTAAAATAATCAAAGTAATCGAAACATTTGACGGTTATAAGGATTTAAACTGCTTAGCCAGTTGGCTATTATTTAGCGGTCAGCAAGTCGCAACACTTAGTGATCTTTATCATAAAGATTTTTGGAATTGTATTAGAAAATCAGATTATCCAAATGCCCATGGTTATTTGGATGGTATAGAGATTACCAATGAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTtgtg                                                                                                                                                                                                                  >  1:551408/1‑251 (MQ=255)                     taatCAAAGTAATCGAAACATTTGACGGTTATAAGGATTTAAACTGCTTAGCCAGTTGGCTATTATTTAGCGGTCAGCAAGTCGCAACACTTAGTGATCTTTATCATAAAGATTTTTGGAATTGTATTAGAAAATCAGATTATCCAAATGCCCATGGTTATTTGGATGGTATAGAGATTACCAATGAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTtg                                                                                                                                                                                                                    <  1:785725/240‑1 (MQ=255)                                         tttGACGGTTATAAGGATTTAAACTGCTTAGCCAGTTGGCTATTATTTAGCGGTCAGCAAGTCGCAACACTTAGTGATCTTTATCATAAAGATTTTTGGAATTGTATTAGAAAATCAGATTATCCAAATGCCCATGGTTATTTGGATGGTATAGAGATTACCAATGAATCATTCCACACGTTACTTCCGCGTTTTATCa                                                                                                                                                                                                                                         <  1:547277/199‑1 (MQ=14)                                                                                                                                                                               tGGTTATTTGGATGGTATAGAGATTACCAATGAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAgg                                               >  1:362020/1‑251 (MQ=11)                                                                                                                                                                                             gTATAGAGATTACCAATGAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAg                                                >  1:209066/1‑236 (MQ=11)                                                                                                                                                                                             gTATAGAGATTACCAATGAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAGGATAAAGTCaataat                                 >  1:240976/1‑251 (MQ=14)                                                                                                                                                                                             gTATAGAGATTACCAATGAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAGGATAAAGTCaataat                                 >  1:38697/1‑251 (MQ=14)                                                                                                                                                                                             gTATAGAGATTACCAATGAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAGGATAAAGTCaataat                                 >  1:174486/1‑251 (MQ=14)                                                                                                                                                                                             gTATAGAGATTACCAATGAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAGGATAAAGTCaataat                                 >  1:860379/1‑251 (MQ=14)                                                                                                                                                                                             gTATAGAGATTACCAATGAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAGGATAAAGTCaataat                                 >  1:512813/1‑251 (MQ=14)                                                                                                                                                                                             gTATAGAGATTACCAATGAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAGGATAAAGTCaataat                                 >  1:542332/1‑251 (MQ=14)                                                                                                                                                                                             gTATAGAGATTACCAATGAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAGGATAAAGTCaataat                                 >  1:750506/1‑251 (MQ=14)                                                                                                                                                                                                      ttACCAATGAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAGGATAAAGTCAATAATTggcaggca                        >  1:870993/1‑251 (MQ=18)                                                                                                                                                                                                         ccAATGAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAGGATAAAGTCAATAATTGGCAGGCAttt                     >  1:394526/1‑251 (MQ=18)                                                                                                                                                                                                                    ttCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAGGATAAAGTCaataat                                 >  1:826012/1‑228 (MQ=17)                                                                                                                                                                                                                           cGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAGGATAAAGTCAATAATTGGCAGGCATTTGATGGTGCACAAAGAAtt   >  1:749754/1‑251 (MQ=37)                                                                                                                                                                                                                           cGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAGGATAAAGTCAATAATTGGCAGGCATTTGATGGTGCACAAAGAAtt   >  1:85443/1‑251 (MQ=37)                                                                                                                                                                                                                           cGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAGGATAAAGTCAATAATTGGCAGGCATTTGATGGTGCACAAAGAAtt   >  1:406892/1‑251 (MQ=37)                                                                                                                                                                                                                             ttACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAGGATAAAGTCAATAATTGGCAGGCATTTGATGGTGCACAAAGAATTa  >  1:527464/1‑250 (MQ=37)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   TCATCTTCTCAAAACTAAAATAATCAAAGTAATCGAAACATTTGACGGTTATAAGGATTTAAACTGCTTAGCCAGTTGGCTATTATTTAGCGGTCAGCAAGTCGCAACACTTAGTGATCTTTATCATAAAGATTTTTGGAATTGTATTAGAAAATCAGATTATCCAAATGCCCATGGTTATTTGGATGGTATAGAGATTACCAATGAATCATTCCACACGTTACTTCTGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAGGATAAAGTCAATAATTGGCAGGCATTTGATGGTGCACAAAGAATTA  >  1/857996‑858465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑