Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	856,238	C→G	T505T (ACC→ACG)	unknown →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	856,238	0	C	G	100.0%	93.4 / NA	34	T505T (ACC→ACG)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (11/23);  total (11/23)

TTGACGTGGTGTTCCGAGTTGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGACGTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGATTCCGCGC‑GCTTGTTTGAGGTGCCATATCACGTACTCCCGCTTGTGCTATCGGACGCGGAGCGCGCATTTATCAAGCCGACCGACTGTTTTGTCTGGAGCTTGGGCGCGCAACCGACTGCACTTTCTGTCGGCTATGACTTGATAGTTGATGCGGGC  >  1/855997‑856313                                                                                                                                                                                                                                                    |                                                                            ttgAGGTGGTGTTCGGAGTTGGGGAGTTTAAAAGCGGCAACGGGGGCGAGT‑TTGTGGCGACGTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGttt                                                                     <  1:415614/250‑1 (MQ=255) ttgAGGTGGTGTTCCGAGTTGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGCCGTGCTTGCGGGATTTTTACGGAATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGttt                                                                     <  1:616932/250‑1 (MQ=255)    aCGTGGTGTTCCGAGTTGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGACGTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGGTTATTAAGCCGACGGATTGGTTTGta                                                                 >  1:307964/1‑250 (MQ=255)       tggtgTTCCGAGTTGGGGAGCTAAAAACCGGCAACGGGGGCGAGT‑TTGGGGCGACGTGCTTGCAGGATGTTTCCGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATgg                                                              <  1:816003/251‑1 (MQ=255)           gtTCCGAGTTGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGACGTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGc                                                           >  1:270186/1‑250 (MQ=255)           gtTCCGAGTTGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGACGTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGc                                                           >  1:294858/1‑250 (MQ=255)                gggTTGGGGAGCTTAAAACCGGCAACGGGGGCGAGT‑TTGTGGCGACGTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTggg                                                      <  1:575099/248‑1 (MQ=255)                        gAGCTTAAAAACGGCAACGGGGGCGAGA‑TTGGGGCGACGTGCTGGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAAc                                              <  1:524924/250‑1 (MQ=255)                           tgtAAAAACGGAAACGGGGGCG‑GATTTGTGTCGCCGTTCTTGCAGGATGTTTACGACATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACGCCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGAc                                          <  1:507157/249‑1 (MQ=255)                                      cAACGGGGGCGAGA‑TTGTGGCGACGTGCTTGCAGGTTTTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTcgg                               <  1:224377/251‑3 (MQ=255)                                      cAACGAGGGCGAGA‑TTGTGGCGACGTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTcgg                               >  1:736308/1‑249 (MQ=255)                                       aaCGGGGGCGAGA‑TTGTGGCGACGTGCTTGCAGGATTTTTACGGCATCCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTcggt                              <  1:616503/251‑4 (MQ=255)                                                 aga‑TTGTGGCGACGTGCTTGCAGGATGTTTCCGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGa                     <  1:534190/250‑1 (MQ=255)                                                  agtttggggagccggccTTGCAGGATGTTCACGCAAGGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGa                     <  1:429749/234‑1 (MQ=255)                                                   gtttGTGGCGACGTGCTTGCAGGATTTTTCCGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGc                                                           <  1:399696/209‑1 (MQ=255)                                                   gttggtgGCGACGTGCTGGAAGGATGTTTAGGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGat                    <  1:685481/246‑2 (MQ=255)                                                   a‑TTGGGGCGAGGTGCTTGCAGGATTTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGata                   <  1:493069/250‑3 (MQ=255)                                                   a‑TTGGGGCGACGTGCTTGCAGGATGTTTCCGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGat                    <  1:330367/249‑2 (MQ=255)                                                   a‑TTGGGGCGACGTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGat                    <  1:792839/249‑2 (MQ=255)                                                      tgtgGCGCCGTGCTTGCAGGATGTTACCGGCAGGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGatatgc                <  1:123973/251‑6 (MQ=255)                                                      tgtgGCGACGTGCTGGCAGGATTTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGatatgc                <  1:336286/251‑6 (MQ=255)                                                       gtgGCGACGTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCATGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGatatg                 >  1:431013/1‑245 (MQ=255)                                                         ggCGACGTGCTTGCAGGATGTTTCCGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGatatgctg              <  1:885890/250‑8 (MQ=255)                                                                tGCTTGCAGGATGTTTACGGCTTGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTTTGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGat                    <  1:114049/237‑2 (MQ=255)                                                                tGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTcggtgggc                          >  1:251048/1‑224 (MQ=255)                                                                    tGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGatatgctggttaatgtgggt  >  1:851526/1‑232 (MQ=255)                                                                            gTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGtat                                                                <  1:246106/181‑3 (MQ=255)                                                                            gTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGat                    >  1:587443/1‑224 (MQ=255)                                                                                            ccgcTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGata                   <  1:87667/210‑3 (MQ=255)                                                                                                tAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGa                     >  1:7335/1‑204 (MQ=255)                                                                                                   ctactCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGa                     <  1:715757/201‑1 (MQ=255)                                                                                                        cAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAGCCGACAGCGCttt                                  >  1:446435/1‑183 (MQ=255)                                                                                                          acacAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGgcg                                                    <  1:750627/163‑1 (MQ=255)                                                                                                                            cTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTggg                                                      >  1:650996/1‑143 (MQ=255)                                                                                                                                                                                                                                                    |                                                                            TTGACGTGGTGTTCCGAGTTGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGACGTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGATTCCGCGC‑GCTTGTTTGAGGTGCCATATCACGTACTCCCGCTTGTGCTATCGGACGCGGAGCGCGCATTTATCAAGCCGACCGACTGTTTTGTCTGGAGCTTGGGCGCGCAACCGACTGCACTTTCTGTCGGCTATGACTTGATAGTTGATGCGGGC  >  1/855997‑856313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑