Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	856,208	G→C	S495S (TCG→TCC)	unknown →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	856,208	0	G	C	100.0%	103.7 / NA	39	S495S (TCG→TCC)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (13/26);  total (13/26)

CCGACCGCGATATTGTTGACGTGGTGTTCCGAGTTGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGACGTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGATTCCGCGC‑GCTTGTTTGAGGTGCCATATCACGTACTCCCGCTTGTGCTATCGGACGCGGAGCGCGCATTTATCAAGCCGACCGACTGTTTTGTCTGGAGCTTGGGCGCGCAACCGACTGCACTTTCTGTCGGCTATGACTTGATAGTTGATGCGGGC  >  1/855982‑856313                                                                                                                                                                                                                                     |                                                                                                          ccGACCGCGATATTGTTGACGGGGTGTTCCGAGTTGGGGAGCTTAAAACCGGCAACGAGGGCGAGA‑TTGTGGCGACGTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAg                                                                                   <  1:205158/251‑1 (MQ=255)    cccGCGATATTGTTGACGTGGTGTTCCGAGTGGGGGAGCTAAAAACCGGCAACGGGGGCGAGA‑TTGGGGCGACGTGCTTGGAGGATGTTTACGGCAGGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGcc                                                                                 <  1:128418/249‑1 (MQ=255)          attttgttgCCGTGGTGTTCCGAGTGGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGACGTGCTTGCAGGATGTTTCCGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAgcgtgcgtt                                                                                          <  1:838062/232‑7 (MQ=255)          atatTGTTGACGTGGTGTTCCGAGTTGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGACGTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGccgacggat                                                                          >  1:477199/1‑247 (MQ=255)            atTGTTGACGTGGTGTTCCGAGTTGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGACGTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGccgacggattg                                                                        >  1:863034/1‑245 (MQ=255)                ttgAGGTGGTGTTCGGAGTTGGGGAGTTTAAAAGCGGCAACGGGGGCGAGT‑TTGTGGCGACGTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGttt                                                                     <  1:415614/250‑1 (MQ=255)                ttgAGGTGGTGTTCCGAGTTGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGCCGTGCTTGCGGGATTTTTACGGAATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGttt                                                                     <  1:616932/250‑1 (MQ=255)                   aCGTGGTGTTCCGAGTTGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGACGTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGGTTATTAAGCCGACGGATTGGTTTGta                                                                 >  1:307964/1‑250 (MQ=255)                      tggtgTTCCGAGTTGGGGAGCTAAAAACCGGCAACGGGGGCGAGT‑TTGGGGCGACGTGCTTGCAGGATGTTTCCGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATgg                                                              <  1:816003/251‑1 (MQ=255)                          gtTCCGAGTTGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGACGTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGc                                                           >  1:270186/1‑250 (MQ=255)                          gtTCCGAGTTGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGACGTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGc                                                           >  1:294858/1‑250 (MQ=255)                               gggTTGGGGAGCTTAAAACCGGCAACGGGGGCGAGT‑TTGTGGCGACGTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTggg                                                      <  1:575099/248‑1 (MQ=255)                                       gAGCTTAAAAACGGCAACGGGGGCGAGA‑TTGGGGCGACGTGCTGGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAAc                                              <  1:524924/250‑1 (MQ=255)                                          tgtAAAAACGGAAACGGGGGCG‑GATTTGTGTCGCCGTTCTTGCAGGATGTTTACGACATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACGCCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGAc                                          <  1:507157/249‑1 (MQ=255)                                                     cAACGGGGGCGAGA‑TTGTGGCGACGTGCTTGCAGGTTTTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTcgg                               <  1:224377/251‑3 (MQ=255)                                                     cAACGAGGGCGAGA‑TTGTGGCGACGTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTcgg                               >  1:736308/1‑249 (MQ=255)                                                      aaCGGGGGCGAGA‑TTGTGGCGACGTGCTTGCAGGATTTTTACGGCATCCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTcggt                              <  1:616503/251‑4 (MQ=255)                                                                aga‑TTGTGGCGACGTGCTTGCAGGATGTTTCCGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGa                     <  1:534190/250‑1 (MQ=255)                                                                 agtttggggagccggccTTGCAGGATGTTCACGCAAGGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGa                     <  1:429749/234‑1 (MQ=255)                                                                  gtttGTGGCGACGTGCTTGCAGGATTTTTCCGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGc                                                           <  1:399696/209‑1 (MQ=255)                                                                  gttggtgGCGACGTGCTGGAAGGATGTTTAGGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGat                    <  1:685481/246‑2 (MQ=255)                                                                  a‑TTGGGGCGAGGTGCTTGCAGGATTTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGata                   <  1:493069/250‑3 (MQ=255)                                                                  a‑TTGGGGCGACGTGCTTGCAGGATGTTTCCGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGat                    <  1:330367/249‑2 (MQ=255)                                                                  a‑TTGGGGCGACGTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGat                    <  1:792839/249‑2 (MQ=255)                                                                     tgtgGCGCCGTGCTTGCAGGATGTTACCGGCAGGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGatatgc                <  1:123973/251‑6 (MQ=255)                                                                     tgtgGCGACGTGCTGGCAGGATTTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGatatgc                <  1:336286/251‑6 (MQ=255)                                                                      gtgGCGACGTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCATGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGatatg                 >  1:431013/1‑245 (MQ=255)                                                                        ggCGACGTGCTTGCAGGATGTTTCCGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGatatgctg              <  1:885890/250‑8 (MQ=255)                                                                               tGCTTGCAGGATGTTTACGGCTTGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTTTGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGat                    <  1:114049/237‑2 (MQ=255)                                                                               tGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTcggtgggc                          >  1:251048/1‑224 (MQ=255)                                                                                   tGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGatatgctggttaatgtgggt  >  1:851526/1‑232 (MQ=255)                                                                                           gTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGtat                                                                <  1:246106/181‑3 (MQ=255)                                                                                           gTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGat                    >  1:587443/1‑224 (MQ=255)                                                                                                           ccgcTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGata                   <  1:87667/210‑3 (MQ=255)                                                                                                               tAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGa                     >  1:7335/1‑204 (MQ=255)                                                                                                                  ctactCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGa                     <  1:715757/201‑1 (MQ=255)                                                                                                                       cAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAGCCGACAGCGCttt                                  >  1:446435/1‑183 (MQ=255)                                                                                                                         acacAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGgcg                                                    <  1:750627/163‑1 (MQ=255)                                                                                                                                           cTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTggg                                                      >  1:650996/1‑143 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                          CCGACCGCGATATTGTTGACGTGGTGTTCCGAGTTGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGACGTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGATTCCGCGC‑GCTTGTTTGAGGTGCCATATCACGTACTCCCGCTTGTGCTATCGGACGCGGAGCGCGCATTTATCAAGCCGACCGACTGTTTTGTCTGGAGCTTGGGCGCGCAACCGACTGCACTTTCTGTCGGCTATGACTTGATAGTTGATGCGGGC  >  1/855982‑856313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑