Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	856,190	A→G	V489V (GTA→GTG)	unknown →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	856,190	0	A	G	100.0%	147.1 / NA	52	V489V (GTA→GTG)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/34);  total (18/34)

CCCGGGTGATGTTATCCAGGTTAATCTGCCCGACCGCGATATTGTTGACGTGGTGTTCCGAGTTGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGA‑C‑GTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGATTCCGCGC‑GCTTGTTTGAGGTGCCATATCACGTACTCCCGCTTGTGCTATCGGACGCGGAGCGCGCATTTATCAAGCCGACCGACTGTTTTGTCTGGAGCTTGGGCGCGCAACCGACTGCACTTTCTGTCGGCTATGACTTGATAGTTGATGCGGGC  >  1/855953‑856313                                                                                                                                                                                                                                                  |                                                                                                                            ccggggTGATGTTATCCGGGTTAATCTGCCCGACCGCGATTTTGTTGCCGTGGTGTTCCGAGTTGGGGAGCTAAAAACCGGCAAGGGGGGCGAGA‑TTGTGGCGA‑C‑GTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGc                                                                                                                <  1:892609/248‑1 (MQ=255) cccGGGTGTTGTTATCCGGGTTATTCGGCCCGACCGCGATTTTGTTGCCGTGGTGTGCCGAGTTGGGGACCTTAAAACCGGCAACGAGGGCGAGA‑TTGTGGCGA‑C‑GTGCTTGCAGGATGTTTACGGCAGGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGc                                                                                                                <  1:10662/251‑1 (MQ=255)    gggTGATGTTATCCGGGTTAATCTGCCCGACCGCGATATTGTTGACGTGGTGTTCCGAGTTGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGA‑C‑GTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTAt                                                                                                             >  1:704171/1‑251 (MQ=255)    gggTGATGTTATCCGGGTTAATCTGCCCGACCGCGATATTGTTGACGTGGTGTTCCGAGTTGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGA‑C‑GTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTAt                                                                                                             >  1:787988/1‑251 (MQ=255)     ggTGATGTTATCCGGGTTAATCTGCCCGACCGCGATATTGTTGACGTGGTGTTCCGAGTTGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGA‑C‑GTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTAt                                                                                                             >  1:312338/1‑250 (MQ=255)      gTGATGTTATCCGGGTTAATCTGCCCGACCGGGATATTGTTGACGTGGTGTTCCGAGTTGGGGAGCTAAAAAACGGCAACGAGGGCGAGT‑TTGGGGCGA‑C‑GTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATc                                                                                                            <  1:430407/250‑1 (MQ=255)       tGATGTTATCCGGGTTAATCTGCCCGCCCGCGATATTGTTGACGTGGTGTTCCGAGTTGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGA‑C‑GTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATcc                                                                                                           <  1:273874/250‑2 (MQ=255)       tGATGTTATCCGGGTTAATCTGCCCGACCGCGATATTGTGGACGTGGTGTTCCGAGTTGGGGAGCTTAAAAACGGCAACGGGGGCGAGA‑TGGGGGCGA‑C‑GTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATcc                                                                                                           <  1:138415/250‑2 (MQ=255)        tataaaccccggattAATCTCCCCACCCGCGATATTGTAGACATGGTGTTCAGATTGGGGGAACTTAAAAGCGGCAGCAGGGGCCAGA‑TTGTGG‑GATCTGTG‑TGGCAGGATTTTTACGGCGGGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATccg                                                                                                          <  1:706407/237‑3 (MQ=255)         aTGTTATCCGGGTTAATCTGCCCGACCGCGATATTGTTGACGTGGTGTTCCGAGTTGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGA‑C‑GTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATccgat                                                                                                        >  1:84447/1‑247 (MQ=255)           tttacgcgggTTAACCTGCCCGACCGCGATTTTGTGAACGTGGTGTACAGATTGGGGGAGCATAAAAACGTAAACGAGGGCGAGA‑TCGTGGCGA‑C‑GTGCTTGAAGGATGTTATCGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATccgatgc                                                                                                      <  1:857370/243‑7 (MQ=255)                              ccGACCGCGATATTGTTGACGGGGTGTTCCGAGTTGGGGAGCTTAAAACCGGCAACGAGGGCGAGA‑TTGTGGCGA‑C‑GTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAg                                                                                   <  1:205158/251‑1 (MQ=255)                                 cccGCGATATTGTTGACGTGGTGTTCCGAGTGGGGGAGCTAAAAACCGGCAACGGGGGCGAGA‑TTGGGGCGA‑C‑GTGCTTGGAGGATGTTTACGGCAGGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGcc                                                                                 <  1:128418/249‑1 (MQ=255)                                       attttgttgCCGTGGTGTTCCGAGTGGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGA‑C‑GTGCTTGCAGGATGTTTCCGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAgcgtgcgtt                                                                                          <  1:838062/232‑7 (MQ=255)                                       atatTGTTGACGTGGTGTTCCGAGTTGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGA‑C‑GTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGccgacggat                                                                          >  1:477199/1‑247 (MQ=255)                                         atTGTTGACGTGGTGTTCCGAGTTGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGA‑C‑GTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATcc                                                                                                           >  1:10003/1‑215 (MQ=255)                                         atTGTTGACGTGGTGTTCCGAGTTGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGA‑C‑GTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGccgacggattg                                                                        >  1:863034/1‑245 (MQ=255)                                             ttgAGGTGGTGTTCGGAGTTGGGGAGTTTAAAAGCGGCAACGGGGGCGAGT‑TTGTGGCGA‑C‑GTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGttt                                                                     <  1:415614/250‑1 (MQ=255)                                             ttgAGGTGGTGTTCCGAGTTGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGC‑C‑GTGCTTGCGGGATTTTTACGGAATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGttt                                                                     <  1:616932/250‑1 (MQ=255)                                                aCGTGGTGTTCCGAGTTGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGA‑C‑GTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGGTTATTAAGCCGACGGATTGGTTTGta                                                                 >  1:307964/1‑250 (MQ=255)                                                   tggtgTTCCGAGTTGGGGAGCTAAAAACCGGCAACGGGGGCGAGT‑TTGGGGCGA‑C‑GTGCTTGCAGGATGTTTCCGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATgg                                                              <  1:816003/251‑1 (MQ=255)                                                       gtTCCGAGTTGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGA‑C‑GTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGc                                                           >  1:294858/1‑250 (MQ=255)                                                       gtTCCGAGTTGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGA‑C‑GTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGc                                                           >  1:270186/1‑250 (MQ=255)                                                            gggTTGGGGAGCTTAAAACCGGCAACGGGGGCGAGT‑TTGTGGCGA‑C‑GTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTggg                                                      <  1:575099/248‑1 (MQ=255)                                                                    gAGCTTAAAAACGGCAACGGGGGCGAGA‑TTGGGGCGA‑C‑GTGCTGGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAAc                                              <  1:524924/250‑1 (MQ=255)                                                                       tgtAAAAACGGAAACGGGGGCG‑GATTTGTGTCGC‑C‑GTTCTTGCAGGATGTTTACGACATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACGCCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGAc                                          <  1:507157/249‑1 (MQ=255)                                                                                  cAACGGGGGCGAGA‑TTGTGGCGA‑C‑GTGCTTGCAGGTTTTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTcgg                               <  1:224377/251‑3 (MQ=255)                                                                                  cAACGAGGGCGAGA‑TTGTGGCGA‑C‑GTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTcgg                               >  1:736308/1‑249 (MQ=255)                                                                                   aaCGGGGGCGAGA‑TTGTGGCGA‑C‑GTGCTTGCAGGATTTTTACGGCATCCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTcggt                              <  1:616503/251‑4 (MQ=255)                                                                                             aga‑TTGTGGCGA‑C‑GTGCTTGCAGGATGTTTCCGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGa                     <  1:534190/250‑1 (MQ=255)                                                                                               gtttGTGGCGA‑C‑GTGCTTGCAGGATTTTTCCGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGc                                                           <  1:399696/209‑1 (MQ=255)                                                                                               gttggtgGCGA‑C‑GTGCTGGAAGGATGTTTAGGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGat                    <  1:685481/246‑2 (MQ=255)                                                                                               a‑TTGGGGCGA‑G‑GTGCTTGCAGGATTTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGata                   <  1:493069/250‑3 (MQ=255)                                                                                               a‑TTGGGGCGA‑C‑GTGCTTGCAGGATGTTTCCGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGat                    <  1:330367/249‑2 (MQ=255)                                                                                               a‑TTGGGGCGA‑C‑GTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGat                    <  1:792839/249‑2 (MQ=255)                                                                                                agtttggggagccggccTTGCAGGATGTTCACGCAAGGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGa                     <  1:429749/234‑1 (MQ=255)                                                                                                  tgtgGCGC‑C‑GTGCTTGCAGGATGTTACCGGCAGGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGatatgc                <  1:123973/251‑6 (MQ=255)                                                                                                  tgtgGCGA‑C‑GTGCTGGCAGGATTTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGatatgc                <  1:336286/251‑6 (MQ=255)                                                                                                   gtgGCGA‑C‑GTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCATGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGatatg                 >  1:431013/1‑245 (MQ=255)                                                                                                     ggCGA‑C‑GTGCTTGCAGGATGTTTCCGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGatatgctg              <  1:885890/250‑8 (MQ=255)                                                                                                              tGCTTGCAGGATGTTTACGGCTTGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTTTGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGat                    <  1:114049/237‑2 (MQ=255)                                                                                                              tGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTcggtgggc                          >  1:251048/1‑224 (MQ=255)                                                                                                                  tGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGatatgctggttaatgtgggt  >  1:851526/1‑232 (MQ=255)                                                                                                                          gTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGtat                                                                <  1:246106/181‑3 (MQ=255)                                                                                                                          gTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGat                    >  1:587443/1‑224 (MQ=255)                                                                                                                                          ccgcTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGata                   <  1:87667/210‑3 (MQ=255)                                                                                                                                              tAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGa                     >  1:7335/1‑204 (MQ=255)                                                                                                                                                 ctactCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAACCGACAGCGCTTTCGGTGGGCTATGa                     <  1:715757/201‑1 (MQ=255)                                                                                                                                                      cAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGGCGCGCAGCCGACAGCGCttt                                  >  1:446435/1‑183 (MQ=255)                                                                                                                                                        acacAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTGGgcg                                                    <  1:750627/163‑1 (MQ=255)                                                                                                                                                                 ggCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATccgatgc                                                                                                      <  1:173238/104‑7 (MQ=255)                                                                                                                                                                          cTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGAGCAGGCGCGGCTT‑TTCGAGGTGCCGTATCACGTGCTACCGCTGGTGCTATCCGATGCCGAGCGTGCGTTTATTAAGCCGACGGATTGTTTTGTATGGAGCCTggg                                                      >  1:650996/1‑143 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                            CCCGGGTGATGTTATCCAGGTTAATCTGCCCGACCGCGATATTGTTGACGTGGTGTTCCGAGTTGGGGAGCTTAAAAACGGCAACGAGGGCGAGA‑TTGTGGCGA‑C‑GTGCTTGCAGGATGTTTACGGCATGCCCGCCGCTAACTACTCAACACAAAAAGGCGAGTCGCTTTACGTGCCGCCCGATTACACCGCCAAACCGATTGATTCCGCGC‑GCTTGTTTGAGGTGCCATATCACGTACTCCCGCTTGTGCTATCGGACGCGGAGCGCGCATTTATCAAGCCGACCGACTGTTTTGTCTGGAGCTTGGGCGCGCAACCGACTGCACTTTCTGTCGGCTATGACTTGATAGTTGATGCGGGC  >  1/855953‑856313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑