BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	855,482	G→A	T253T (ACG→ACA)	unknown →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	855,482	0	G	A	99.0%	259.7 / NA	97	T253T (ACG→ACA)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (45/51); total (45/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CGGGTGCGCCGTGCCCACAAAGGCTGGCACAATGGCGTGGTATGGTACGCCGAAAAAGCCAAAATCCTGCTGCGCAATGACAATCTTAAAATTAAAGGTTTGACAGCCGAACAAGAACAAAATGTACGCCAAATCCACGCCATGAACCCGGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCAGAGCTGGATTTAGACAGCTATAAAAAAGCGGCAGATACGCTTTTCGATGAGGGATTTGGCCTCTGTATCCGCTACAACCGGCAAACCTCCATTAAAGATTTTATTAAGCAAATTGTTGATCATATCGGGGCGGCGCAATACGACAATGTGGAGACCGGTAAACAGGCGATTAAATTGATTCGGCAAGACTACAATGTAGCGGATTTACCGCTGTTTAGCTATGACAAAGGGATTGTTGCGGTGCTTGATGATGACAGCGCCGCAACCGACAAG  >  1/855234‑855716

cgtctGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACtttt                                                                                                                                                                                                                                       >  1:159898/5‑251 (MQ=255)

 gtctGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACtttt                                                                                                                                                                                                                                       >  1:193367/4‑250 (MQ=255)

      cgcCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGacg                                                                                                                                                                                                                                  <  1:80101/250‑3 (MQ=255)

                 caAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGAttcgggt                                                                                                                                                                                                                      <  1:259196/251‑6 (MQ=255)

                 caAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGAttcgggt                                                                                                                                                                                                                      <  1:852080/251‑6 (MQ=255)

                    aGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGAttcgggttgt                                                                                                                                                                                                                   >  1:310029/1‑243 (MQ=255)

                       cTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGAttcgggttgtgt                                                                                                                                                                                                                 <  1:162616/250‑11 (MQ=255)

                             gcacaatggggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGAttcggg                                                                                                                                                                                                                       >  1:356017/10‑237 (MQ=255)

                                caatggggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCt                                                                                                                                                                                                          <  1:847849/245‑1 (MQ=255)

                                  atggggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTAc                                                                                                                                                                                                        <  1:427959/247‑1 (MQ=255)

                                    ggggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACaa                                                                                                                                                                                                      >  1:613288/3‑251 (MQ=255)

                                    ggggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACaa                                                                                                                                                                                                      >  1:578759/3‑251 (MQ=255)

                                     gggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACaat                                                                                                                                                                                                     >  1:644129/2‑250 (MQ=255)

                                      ggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGac                                                                                                                                                                                                                                   <  1:17939/220‑2 (MQ=255)

                                      ggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACaat                                                                                                                                                                                                     >  1:861551/1‑249 (MQ=255)

                                       gtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGAttcgggttgtgt                                                                                                                                                                                                                 <  1:838638/237‑11 (MQ=255)

                                       gtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACaatc                                                                                                                                                                                                    <  1:386890/250‑3 (MQ=255)

                                         atggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTa                                                                                                                                                                                                         <  1:812226/243‑1 (MQ=255)

                                         atggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCgg                                                                                                                                                                                                  >  1:730376/1‑250 (MQ=255)

                                         atggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGCAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCgg                                                                                                                                                                                                  >  1:389850/1‑250 (MQ=255)

                                            gtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCaaa                                                                                                                                                                                              >  1:262542/1‑251 (MQ=255)

                                              accccGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAAcc                                                                                                                                                                                            <  1:695765/248‑1 (MQ=255)

                                              accccGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAAc                                                                                                                                                                                             >  1:889059/4‑250 (MQ=255)

                                                cccGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGAttcggg                                                                                                                                                                                                                       >  1:683668/2‑218 (MQ=255)

                                                        atgcAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGAAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTaagg                                                                                                                                                                                  <  1:842950/248‑3 (MQ=255)

                                                        atgcAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGCCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTaagg                                                                                                                                                                                  >  1:862738/4‑249 (MQ=255)

                                                             aaaTCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTaaggagttt                                                                                                                                                                             >  1:599979/1‑244 (MQ=255)

                                                               atcgtgctgcGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCt                                                                                                                                                                                                          <  1:226205/216‑1 (MQ=255)

                                                               atcgtgctgcGCAATGACAATCGCAAAAGTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACGCTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTttat                                                                                                                                                                           <  1:721069/247‑1 (MQ=255)

                                                                 cgtgctgcGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTttatta                                                                                                                                                                         <  1:359268/249‑1 (MQ=255)

                                                                      tgcGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCaa                                                                                                                                                                    <  1:60118/251‑1 (MQ=255)

                                                                       gcgcAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGAttcgggttgtgta                                                                                                                                                                                                                <  1:334947/206‑12 (MQ=255)

                                                                       gcgcAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGAttcgggt                                                                                                                                                                                                                      <  1:272132/200‑6 (MQ=255)

                                                                       gcgcAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCaaa                                                                                                                                                                   >  1:235553/1‑251 (MQ=255)

                                                                         gcAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAAt                                                                                                                                                                  >  1:29773/1‑250 (MQ=255)

                                                                           aaTGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAAtcgt                                                                                                                                                               >  1:493187/1‑248 (MQ=255)

                                                                           aaTGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAAtcgt                                                                                                                                                               <  1:666102/251‑4 (MQ=255)

                                                                                                               caggaagaaaatttacgtgagaTCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTgg                                                                                                                            <  1:613003/229‑1 (MQ=255)

                                                                                                               caggaagaaaatttacgtgagaTCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTgg                                                                                                                            <  1:556454/229‑1 (MQ=255)

                                                                                                                   aagaaaatttacgtgagaTCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGAcc                                                                                                                       <  1:280600/234‑1 (MQ=255)

                                                                                                                                   gaTCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTa                                                                                                       >  1:179360/2‑251 (MQ=255)

                                                                                                                                   gaTCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTa                                                                                                       >  1:304696/2‑251 (MQ=255)

                                                                                                                                    aTCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTaa                                                                                                      <  1:618595/251‑1 (MQ=255)

                                                                                                                                           cgaTGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGC‑AAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGAtt                                                                                               >  1:850844/3‑250 (MQ=255)

                                                                                                                                                 aCCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGAc                                                                                                                        >  1:642945/1‑220 (MQ=255)

                                                                                                                                                    ccgCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCgccaggac                                                                                      <  1:687703/249‑8 (MQ=255)

                                                                                                                                                    ccgCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCgccaggac                                                                                      <  1:189026/249‑8 (MQ=255)

                                                                                                                                                     cgCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACt                                                                                     <  1:64682/250‑1 (MQ=255)

                                                                                                                                                      gCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGAc                                                                                                                                   >  1:823504/1‑204 (MQ=255)

                                                                                                                                                       cacaTATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGacc                                                                                                                                                          >  1:514635/1‑178 (MQ=255)

                                                                                                                                                       cacaTATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCgg                                                                                                                                                   >  1:603359/1‑187 (MQ=255)

                                                                                                                                                         caTATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACaa                                                                                 <  1:882726/251‑1 (MQ=255)

                                                                                                                                                          atatCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAAt                                                                                <  1:572803/251‑1 (MQ=255)

                                                                                                                                                           tatCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATg                                                                               >  1:744359/1‑251 (MQ=255)

                                                                                                                                                           tatCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATg                                                                               >  1:186549/1‑251 (MQ=255)

                                                                                                                                                            atCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGt                                                                              <  1:59543/251‑1 (MQ=255)

                                                                                                                                                             tCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATAc                                                                                                                                      >  1:342824/1‑194 (MQ=255)

                                                                                                                                                             tCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGt                                                                              >  1:443454/1‑250 (MQ=255)

                                                                                                                                                              ccTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAg                                                                            >  1:548196/1‑251 (MQ=255)

                                                                                                                                                              ccTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAg                                                                            <  1:509379/251‑1 (MQ=255)

                                                                                                                                                                 ggTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGa                                                                                                          <  1:232970/218‑1 (MQ=255)

                                                                                                                                                                 ggTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGCATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGCGCCGCCCAATACGACaa                                                                                                                                 >  1:869522/1‑195 (MQ=255)

                                                                                                                                                                  gTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAGCgg                                                                         <  1:104647/250‑1 (MQ=255)

                                                                                                                                                                          cgcCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAGCGGATTTACCg                                                                 >  1:555048/1‑250 (MQ=255)

                                                                                                                                                                          cgcCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAGCGGATTTACCGc                                                                <  1:286779/251‑1 (MQ=255)

                                                                                                                                                                            ccACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATc                                                                                                                                                     >  1:647640/1‑164 (MQ=255)

                                                                                                                                                                                taataaAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAAt                                                                                >  1:670213/1‑229 (MQ=255)

                                                                                                                                                                                 aataaAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTa                                                                                                                                                                                     <  1:890651/127‑1 (MQ=255)

                                                                                                                                                                                 aataaAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTttatt                                                                                                                                                                          >  1:704704/1‑138 (MQ=255)

                                                                                                                                                                                 aataaAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACaa                                                                                                                                 >  1:250392/1‑179 (MQ=255)

                                                                                                                                                                                 aataaAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTg                                                                                                  <  1:466365/210‑1 (MQ=255)

                                                                                                                                                                                 aataaAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACaa                                                                                 <  1:856626/227‑1 (MQ=255)

                                                                                                                                                                                 aataaAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAGCGGATTTACCGc                                                                <  1:420121/244‑1 (MQ=255)

                                                                                                                                                                                 aataaAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAGCGGATTTACCGCTGTTTAg                                                         >  1:219782/1‑251 (MQ=255)

                                                                                                                                                                                 aataaAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAGCGGATTTACCGCTGTTTAg                                                         >  1:85401/1‑251 (MQ=255)

                                                                                                                                                                                 aataaAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAGCGGATTTACCGCTGTTTAg                                                         >  1:482839/1‑251 (MQ=255)

                                                                                                                                                                                 aataaAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGCTGACCATATCGCTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAGCGGATTTACCGCTGTTTAg                                                         >  1:640564/1‑251 (MQ=255)

                                                                                                                                                                                    aaaaGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAGCGGATTTACCGCTGTTTAGCTa                                                      <  1:144978/251‑1 (MQ=255)

                                                                                                                                                                                    aaaaGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAGCGGATTTACCGCTGTTTAGCTa                                                      <  1:706585/251‑1 (MQ=255)

                                                                                                                                                                                    aaaaGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAGCGGATTTACCGCTGTTTAGCTa                                                      <  1:463050/251‑1 (MQ=255)

                                                                                                                                                                                         cTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAGCGGATTTACCGCTGTTTAGCTATGAc                                                  <  1:248243/250‑1 (MQ=255)

                                                                                                                                                                                         cTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAGCGGATTTACCGCTGTTTAGCTATGAc                                                  <  1:484444/250‑1 (MQ=255)

                                                                                                                                                                                               cggcAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAGCGGATTTACCGCTGTTTAGCTATGACAACggg                                            <  1:113469/250‑1 (MQ=255)

                                                                                                                                                                                                ggcAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAGCGGATTTACCGCTGTTTAGCTATGACAACGGGAt                                          >  1:308314/1‑251 (MQ=255)

                                                                                                                                                                                                 gcAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAGCGGATTTACCGCTGTTTAGCTATGACAACGGGAt                                          <  1:57815/250‑1 (MQ=255)

                                                                                                                                                                                                     aaaaGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAGCGGATTTACCGCTGTTTAGCTATGACAACGGGAttgttg                                     <  1:654789/251‑1 (MQ=255)

                                                                                                                                                                                                          aCTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAGCGGATTTACCGCTGTTTAGCTATGACAACGGGATTGTTGCGGTg                                <  1:753091/251‑1 (MQ=255)

                                                                                                                                                                                                                  gagCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAGCGGATTTACCGCTGTTTAGCTATGACAACGGGATTGTTGCgg                                  >  1:368396/1‑241 (MQ=255)

                                                                                                                                                                                                                      tGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAGCGGATTTACCGCTGTTTAGCTATGACAACGGGATTGTTGCGGTGCTTGATGATGAc                    <  1:123178/251‑1 (MQ=255)

                                                                                                                                                                                                                       ggATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAGCGGATTTACCGCTGTTTAGCTATGACAACGGGATTGTTGCGGTGCTTGATGATGACa                   >  1:400994/1‑251 (MQ=255)

                                                                                                                                                                                                                                   ctacaaaaaaGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAGCGGATTTACCGCTGTTTAGCTATGACAACGGGATTGTTGCGGTGCTTGATGATGACAGCGCCGCAAc         <  1:827565/245‑1 (MQ=255)

                                                                                                                                                                                                                                     acaaaaaaGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAGCGGATTTACCGCTGTTTAGCTATGACAACGGGATTGTTGCGGTGCTTGATGATGAc                    >  1:393520/3‑236 (MQ=255)

                                                                                                                                                                                                                                     acaaaaaaGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAGCGGATTTACCGCTGTTTAGCTATGACAACGGGATTGTTGCGGTGCTTGATGATGACAGCGCCGCAACCGat     <  1:607322/249‑2 (MQ=255)

                                                                                                                                                                                                                                        aaaaaGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAGCGGATTTACCGCTGTTTAGCTATGACAACGGGATTGTTGCGGTGCTTGATGATGACAGCGCCGCAACCGATAAg  <  1:165815/251‑1 (MQ=255)

                                                                                                                                                                                                                                                 ctgATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAGCGGATTTACCGCTGTTTAGCTATGACAACGGGATTGTTGCGGTGCTTGATGATGACAg                  >  1:535797/3‑226 (MQ=255)

                                                                                                                                                                                                                                                    aTACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAGCGGATTTACCGCTGTTTAGCTATGACAACGGGATTGTTGCGGTGCTtgatga                        <  1:604974/217‑1 (MQ=255)

                                                                                                                                                                                                                                                    aTACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAATCGTTGACCATATCGGTGCCGCCCAATACGACAATGTGGAGACCGGCAAGCAGGCGATTAAATTGATTCGCCAGGACTACAATGTAGCGGATTTACCGCTGTTTAGCTATGACAACGGGATTGTTGCGGTGCTTGATGATGAc                    <  1:669944/221‑1 (MQ=255)

CGGGTGCGCCGTGCCCACAAAGGCTGGCACAATGGCGTGGTATGGTACGCCGAAAAAGCCAAAATCCTGCTGCGCAATGACAATCTTAAAATTAAAGGTTTGACAGCCGAACAAGAACAAAATGTACGCCAAATCCACGCCATGAACCCGGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCAGAGCTGGATTTAGACAGCTATAAAAAAGCGGCAGATACGCTTTTCGATGAGGGATTTGGCCTCTGTATCCGCTACAACCGGCAAACCTCCATTAAAGATTTTATTAAGCAAATTGTTGATCATATCGGGGCGGCGCAATACGACAATGTGGAGACCGGTAAACAGGCGATTAAATTGATTCGGCAAGACTACAATGTAGCGGATTTACCGCTGTTTAGCTATGACAAAGGGATTGTTGCGGTGCTTGATGATGACAGCGCCGCAACCGACAAG  >  1/855234‑855716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`