Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	855,320	T→C	L199L (CTT→CTC)	unknown →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	855,320	0	T	C	100.0%	219.9 / NA	77	L199L (CTT→CTC)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (46/31);  total (46/31)

GCACAATAAGTTTTTCCGACCCGGCAATGTCAGCCCCGGTAATGTGGCAACAGATGAGACTATCCCGGGCTTTCGCGGCATTGTCACTACCGTGTTTTCGGGCCTTATCAGCTGCTATAACGCATACCCTAAAAAGCACAGCTACCGGGTGCGCCGTGCCCACAAAGGCTGGCACAATGGCGTGGTATGGTACGCCGAAAAAGCCAAAATCCTGCTGCGCAATGACAATCTTAAAATTAAAGGTTTGACAGCCGAACAAGAACAAAATGTACGCCAAATCCACGCCATGAACCCGGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCAGAGCTGGATTTAGACAGCTATAAAAAAGCGGCAGATACGCTTTTCGATGAGGGATTTGGCCTCTGTATCCGCTACAACCGGCAAACCTCCATTAAAGATTTTATTAAGCAAATTGT  >  1/855089‑855559                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                gcataaTAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTaagggggtaacggcag                                                                                                                                                                                                                           >  1:436180/5‑237 (MQ=255)  cataaTAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTaagggggtaacggc                                                                                                                                                                                                                             <  1:681245/245‑13 (MQ=255)    taaTAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTaagggggtaacggcagagc                                                                                                                                                                                                                        <  1:70762/250‑18 (MQ=255)                                                                               cATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGc                                                                                                                                              <  1:936/250‑1 (MQ=255)                                                                               cATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGc                                                                                                                                              <  1:705772/250‑1 (MQ=255)                                                                                aTTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATaaa                                                                                                                                                 <  1:693491/246‑1 (MQ=255)                                                                                  tGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGc                                                                                                                                              >  1:381427/1‑247 (MQ=255)                                                                                  tGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTggg                                                                                                                                          >  1:840566/1‑251 (MQ=255)                                                                                  tGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTggg                                                                                                                                          >  1:148821/1‑251 (MQ=255)                                                                                  tGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTggg                                                                                                                                          >  1:39040/1‑251 (MQ=255)                                                                                       cTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTGCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCTCATATCCTGGTTGAATGCGCCACTAATAAAAGc                                                                                                                                              >  1:163371/1‑242 (MQ=255)                                                                                       cTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGc                                                                                                                                              >  1:582862/1‑242 (MQ=255)                                                                                            gtgtTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCaaaaa                                                                                                                                >  1:847608/1‑251 (MQ=255)                                                                                            gtgtTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCaaaaa                                                                                                                                >  1:570486/1‑251 (MQ=255)                                                                                                tttCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGa                                                                                                                             >  1:316830/1‑250 (MQ=255)                                                                                                 ttcaggCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACt                                                                                                                           <  1:247708/247‑1 (MQ=255)                                                                                                     ggCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTAt                                                                                                                        >  1:23902/1‑250 (MQ=255)                                                                                                        cTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATccga                                                                                                                    >  1:879461/1‑248 (MQ=255)                                                                                                                  gctATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTa                                                                                                          >  1:11148/1‑251 (MQ=255)                                                                                                                   ctATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAg                                                                                                         >  1:510612/1‑251 (MQ=255)                                                                                                                   ctATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAg                                                                                                         >  1:487576/1‑251 (MQ=255)                                                                                                                   ctATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAg                                                                                                         >  1:241348/1‑251 (MQ=255)                                                                                                                   ctATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAAACTTATCCGAGCTGGATTTAg                                                                                                         >  1:870074/1‑251 (MQ=255)                                                                                                                     ataACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGAc                                                                                                       <  1:876568/251‑1 (MQ=255)                                                                                                                       aaCGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAg                                                                                                     >  1:490477/1‑251 (MQ=255)                                                                                                                         cgcttACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGc                                                                                                                                              >  1:78965/5‑208 (MQ=255)                                                                                                                               cccTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACaaaa                                                                                             >  1:632450/1‑251 (MQ=255)                                                                                                                                 cTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACaaaaaa                                                                                           >  1:432391/1‑251 (MQ=255)                                                                                                                                   aaaaaaCACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATTACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAg                                                                                          >  1:724342/1‑250 (MQ=255)                                                                                                                                      aaacacaGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCgg                                                                                       >  1:410924/4‑250 (MQ=255)                                                                                                                                        acacaGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCgg                                                                                       >  1:528457/2‑248 (MQ=255)                                                                                                                                             gCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGc                                                                                                                                              >  1:107733/1‑188 (MQ=255)                                                                                                                                             gCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGAc                                                                                                       >  1:385703/1‑227 (MQ=255)                                                                                                                                                  cgtctGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGa                                                                                                        <  1:263204/217‑1 (MQ=255)                                                                                                                                                  cgtctGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACtttt                                                                          >  1:159898/5‑251 (MQ=255)                                                                                                                                                   gtctGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACtttt                                                                          >  1:193367/4‑250 (MQ=255)                                                                                                                                                        cgcCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGacg                                                                     <  1:80101/250‑3 (MQ=255)                                                                                                                                                                 ctcaAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGc                                                                                                                                              >  1:212888/3‑168 (MQ=255)                                                                                                                                                                   caAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGAttcgggt                                                         <  1:852080/251‑6 (MQ=255)                                                                                                                                                                   caAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGAttcgggt                                                         <  1:259196/251‑6 (MQ=255)                                                                                                                                                                      aGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGAttcgggttgt                                                      >  1:310029/1‑243 (MQ=255)                                                                                                                                                                         cTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGAttcgggttgtgt                                                    <  1:162616/250‑11 (MQ=255)                                                                                                                                                                               gcacaatggggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGAttcggg                                                          >  1:356017/10‑237 (MQ=255)                                                                                                                                                                                  caatggggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCt                                             <  1:847849/245‑1 (MQ=255)                                                                                                                                                                                    atggggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTAc                                           <  1:427959/247‑1 (MQ=255)                                                                                                                                                                                      ggggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATcc                                                                                                                      <  1:130671/172‑2 (MQ=255)                                                                                                                                                                                      ggggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATAca                                                                               <  1:881058/211‑2 (MQ=255)                                                                                                                                                                                      ggggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACaa                                         >  1:578759/3‑251 (MQ=255)                                                                                                                                                                                      ggggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACaa                                         >  1:613288/3‑251 (MQ=255)                                                                                                                                                                                       gggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGAc                                                                                                       >  1:130852/2‑188 (MQ=255)                                                                                                                                                                                       gggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACaat                                        >  1:644129/2‑250 (MQ=255)                                                                                                                                                                                        ggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGac                                                                      <  1:17939/220‑2 (MQ=255)                                                                                                                                                                                        ggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACaat                                        >  1:861551/1‑249 (MQ=255)                                                                                                                                                                                         gtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGAttcgggttgtgt                                                    <  1:838638/237‑11 (MQ=255)                                                                                                                                                                                         gtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACaatc                                       <  1:386890/250‑3 (MQ=255)                                                                                                                                                                                           atggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATccg                                                                                                                     <  1:308402/170‑3 (MQ=255)                                                                                                                                                                                           atggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTa                                            <  1:812226/243‑1 (MQ=255)                                                                                                                                                                                           atggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCgg                                     >  1:730376/1‑250 (MQ=255)                                                                                                                                                                                           atggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGCAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCgg                                     >  1:389850/1‑250 (MQ=255)                                                                                                                                                                                              gtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACaaaa                                                                                             <  1:895310/191‑1 (MQ=255)                                                                                                                                                                                              gtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCaaa                                 >  1:262542/1‑251 (MQ=255)                                                                                                                                                                                                accccGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAAcc                               <  1:695765/248‑1 (MQ=255)                                                                                                                                                                                                accccGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAAc                                >  1:889059/4‑250 (MQ=255)                                                                                                                                                                                                  cccGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGAttcggg                                                          >  1:683668/2‑218 (MQ=255)                                                                                                                                                                                                          atgcAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGAAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTaagg                     <  1:842950/248‑3 (MQ=255)                                                                                                                                                                                                          atgcAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGCCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTaagg                     >  1:862738/4‑249 (MQ=255)                                                                                                                                                                                                               aaaTCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTaaggagttt                >  1:599979/1‑244 (MQ=255)                                                                                                                                                                                                                 atcgtgctgcGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCt                                             <  1:226205/216‑1 (MQ=255)                                                                                                                                                                                                                 atcgtgctgcGCAATGACAATCGCAAAAGTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACGCTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTttat              <  1:721069/247‑1 (MQ=255)                                                                                                                                                                                                                   cgtgctgcGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTttatta            <  1:359268/249‑1 (MQ=255)                                                                                                                                                                                                                        tgcGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCaa       <  1:60118/251‑1 (MQ=255)                                                                                                                                                                                                                         gcgcAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGAttcgggttgtgta                                                   <  1:334947/206‑12 (MQ=255)                                                                                                                                                                                                                         gcgcAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGAttcgggt                                                         <  1:272132/200‑6 (MQ=255)                                                                                                                                                                                                                         gcgcAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCaaa      >  1:235553/1‑251 (MQ=255)                                                                                                                                                                                                                           gcAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAAt     >  1:29773/1‑250 (MQ=255)                                                                                                                                                                                                                             aaTGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAAtcgt  >  1:493187/1‑248 (MQ=255)                                                                                                                                                                                                                             aaTGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTAAGGAGTTTATTAAGCAAAtcgt  <  1:666102/251‑4 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                GCACAATAAGTTTTTCCGACCCGGCAATGTCAGCCCCGGTAATGTGGCAACAGATGAGACTATCCCGGGCTTTCGCGGCATTGTCACTACCGTGTTTTCGGGCCTTATCAGCTGCTATAACGCATACCCTAAAAAGCACAGCTACCGGGTGCGCCGTGCCCACAAAGGCTGGCACAATGGCGTGGTATGGTACGCCGAAAAAGCCAAAATCCTGCTGCGCAATGACAATCTTAAAATTAAAGGTTTGACAGCCGAACAAGAACAAAATGTACGCCAAATCCACGCCATGAACCCGGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCAGAGCTGGATTTAGACAGCTATAAAAAAGCGGCAGATACGCTTTTCGATGAGGGATTTGGCCTCTGTATCCGCTACAACCGGCAAACCTCCATTAAAGATTTTATTAAGCAAATTGT  >  1/855089‑855559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑