Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	855,300	C→G	L193V (CTG→GTG)	unknown →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	855,300	0	C	G	100.0%	200.2 / NA	73	L193V (CTG→GTG)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (44/29);  total (44/29)

GCGCAAGCGCTTTAAAAAAGACGGGCAGCACAATAAGTTTTTCCGACCCGGCAATGTCAGCCCCGGTAATGTGGCAACAGATGAGACTATCCCGGGCTTTCGCGGCATTGTCACTACCGTGTTTTCGGGCCTTATCAGCTGCTATAACGCATACCCTAAAAAGCACAGCTACCGGGTGCGCCGTGCCCACAAAGGCTGGCACAATGGCGTGGTATGGTACGCCGAAAAAGCCAAAATCCTGCTGCGCAATGACAATCTTAAAATTAAAGGTTTGACAGCCGAACAAGAACAAAATGTACGCCAAATCCACGCCATGAACCCGGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCAGAGCTGGATTTAGACAGCTATAAAAAAGCGGCAGATACGCTTTTCGATGAGGGATTTGGCCTCTGTATCCGCTACAACCGGCAAACCTCCATTAAAGATTTT  >  1/855062‑855545                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      gcgcAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGAc                                                                                                                                                                                                                                         <  1:30435/250‑1 (MQ=255)    cAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATc                                                                                                                                                                                                                                     <  1:865665/251‑1 (MQ=255)    aaggcgcTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTGCCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATc                                                                                                                                                                                                                                     <  1:834943/248‑1 (MQ=255)          cTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGa                                                                                                                                                                                                                                          >  1:841382/1‑240 (MQ=255)              aaaaaaGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGa                                                                                                                                                                                                                                          <  1:515774/236‑1 (MQ=255)                            gcataaTAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTaagggggtaacggcag                                                                                                                                                                                                             >  1:436180/5‑237 (MQ=255)                             cataaTAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTaagggggtaacggc                                                                                                                                                                                                               <  1:681245/245‑13 (MQ=255)                               taaTAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTgcgc                                                                                                                                                                                                                                               <  1:826628/213‑1 (MQ=255)                               taaTAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTaagggggtaacggcagagc                                                                                                                                                                                                          <  1:70762/250‑18 (MQ=255)                                                                                                          cATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGc                                                                                                                                <  1:936/250‑1 (MQ=255)                                                                                                          cATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGc                                                                                                                                <  1:705772/250‑1 (MQ=255)                                                                                                           aTTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATaaa                                                                                                                                   <  1:693491/246‑1 (MQ=255)                                                                                                             tGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGc                                                                                                                                >  1:381427/1‑247 (MQ=255)                                                                                                             tGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTggg                                                                                                                            >  1:840566/1‑251 (MQ=255)                                                                                                             tGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTggg                                                                                                                            >  1:148821/1‑251 (MQ=255)                                                                                                             tGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTggg                                                                                                                            >  1:39040/1‑251 (MQ=255)                                                                                                                  cTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTGCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCTCATATCCTGGTTGAATGCGCCACTAATAAAAGc                                                                                                                                >  1:163371/1‑242 (MQ=255)                                                                                                                  cTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGc                                                                                                                                >  1:582862/1‑242 (MQ=255)                                                                                                                       gtgtTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCaaaaa                                                                                                                  >  1:847608/1‑251 (MQ=255)                                                                                                                       gtgtTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCaaaaa                                                                                                                  >  1:570486/1‑251 (MQ=255)                                                                                                                           tttCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGa                                                                                                               >  1:316830/1‑250 (MQ=255)                                                                                                                            ttcaggCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACt                                                                                                             <  1:247708/247‑1 (MQ=255)                                                                                                                                ggCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTAt                                                                                                          >  1:23902/1‑250 (MQ=255)                                                                                                                                   cTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATccga                                                                                                      >  1:879461/1‑248 (MQ=255)                                                                                                                                             gctATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTa                                                                                            >  1:11148/1‑251 (MQ=255)                                                                                                                                              ctATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAg                                                                                           >  1:241348/1‑251 (MQ=255)                                                                                                                                              ctATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAg                                                                                           >  1:510612/1‑251 (MQ=255)                                                                                                                                              ctATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAg                                                                                           >  1:487576/1‑251 (MQ=255)                                                                                                                                              ctATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAAACTTATCCGAGCTGGATTTAg                                                                                           >  1:870074/1‑251 (MQ=255)                                                                                                                                                ataACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGAc                                                                                         <  1:876568/251‑1 (MQ=255)                                                                                                                                                  aaCGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAg                                                                                       >  1:490477/1‑251 (MQ=255)                                                                                                                                                    cgcttACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGc                                                                                                                                >  1:78965/5‑208 (MQ=255)                                                                                                                                                          cccTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACaaaa                                                                               >  1:632450/1‑251 (MQ=255)                                                                                                                                                            cTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACaaaaaa                                                                             >  1:432391/1‑251 (MQ=255)                                                                                                                                                              aaaaaaCACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATTACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAg                                                                            >  1:724342/1‑250 (MQ=255)                                                                                                                                                                 aaacacaGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCgg                                                                         >  1:410924/4‑250 (MQ=255)                                                                                                                                                                   acacaGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCgg                                                                         >  1:528457/2‑248 (MQ=255)                                                                                                                                                                        gCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGc                                                                                                                                >  1:107733/1‑188 (MQ=255)                                                                                                                                                                        gCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGAc                                                                                         >  1:385703/1‑227 (MQ=255)                                                                                                                                                                             cgtctGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGa                                                                                          <  1:263204/217‑1 (MQ=255)                                                                                                                                                                             cgtctGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACtttt                                                            >  1:159898/5‑251 (MQ=255)                                                                                                                                                                              gtctGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACtttt                                                            >  1:193367/4‑250 (MQ=255)                                                                                                                                                                                   cgcCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGacg                                                       <  1:80101/250‑3 (MQ=255)                                                                                                                                                                                            ctcaAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGc                                                                                                                                >  1:212888/3‑168 (MQ=255)                                                                                                                                                                                              caAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGAttcgggt                                           <  1:259196/251‑6 (MQ=255)                                                                                                                                                                                              caAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGAttcgggt                                           <  1:852080/251‑6 (MQ=255)                                                                                                                                                                                                 aGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGAttcgggttgt                                        >  1:310029/1‑243 (MQ=255)                                                                                                                                                                                                    cTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGAttcgggttgtgt                                      <  1:162616/250‑11 (MQ=255)                                                                                                                                                                                                          gcacaatggggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGAttcggg                                            >  1:356017/10‑237 (MQ=255)                                                                                                                                                                                                             caatggggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCt                               <  1:847849/245‑1 (MQ=255)                                                                                                                                                                                                               atggggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTAc                             <  1:427959/247‑1 (MQ=255)                                                                                                                                                                                                                 ggggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATcc                                                                                                        <  1:130671/172‑2 (MQ=255)                                                                                                                                                                                                                 ggggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATAca                                                                 <  1:881058/211‑2 (MQ=255)                                                                                                                                                                                                                 ggggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACaa                           >  1:613288/3‑251 (MQ=255)                                                                                                                                                                                                                 ggggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACaa                           >  1:578759/3‑251 (MQ=255)                                                                                                                                                                                                                  gggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGAc                                                                                         >  1:130852/2‑188 (MQ=255)                                                                                                                                                                                                                  gggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACaat                          >  1:644129/2‑250 (MQ=255)                                                                                                                                                                                                                   ggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGac                                                        <  1:17939/220‑2 (MQ=255)                                                                                                                                                                                                                   ggtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACaat                          >  1:861551/1‑249 (MQ=255)                                                                                                                                                                                                                    gtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGAttcgggttgtgt                                      <  1:838638/237‑11 (MQ=255)                                                                                                                                                                                                                    gtatggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACaatc                         <  1:386890/250‑3 (MQ=255)                                                                                                                                                                                                                      atggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATccg                                                                                                       <  1:308402/170‑3 (MQ=255)                                                                                                                                                                                                                      atggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTa                              <  1:812226/243‑1 (MQ=255)                                                                                                                                                                                                                      atggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCgg                       >  1:730376/1‑250 (MQ=255)                                                                                                                                                                                                                      atggtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGCAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCgg                       >  1:389850/1‑250 (MQ=255)                                                                                                                                                                                                                         gtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACaaaa                                                                               <  1:895310/191‑1 (MQ=255)                                                                                                                                                                                                                         gtaCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCaaa                   >  1:262542/1‑251 (MQ=255)                                                                                                                                                                                                                           accccGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAAcc                 <  1:695765/248‑1 (MQ=255)                                                                                                                                                                                                                           accccGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAAc                  >  1:889059/4‑250 (MQ=255)                                                                                                                                                                                                                             cccGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGAttcggg                                            >  1:683668/2‑218 (MQ=255)                                                                                                                                                                                                                                     atgcAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGAAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTaagg       <  1:842950/248‑3 (MQ=255)                                                                                                                                                                                                                                     atgcAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGCCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTaagg       >  1:862738/4‑249 (MQ=255)                                                                                                                                                                                                                                          aaaTCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGACGAGGGATTCGGGTTGTGTATCCGCTACAATCGGCAAACCTCAATTaaggagttt  >  1:599979/1‑244 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      GCGCAAGCGCTTTAAAAAAGACGGGCAGCACAATAAGTTTTTCCGACCCGGCAATGTCAGCCCCGGTAATGTGGCAACAGATGAGACTATCCCGGGCTTTCGCGGCATTGTCACTACCGTGTTTTCGGGCCTTATCAGCTGCTATAACGCATACCCTAAAAAGCACAGCTACCGGGTGCGCCGTGCCCACAAAGGCTGGCACAATGGCGTGGTATGGTACGCCGAAAAAGCCAAAATCCTGCTGCGCAATGACAATCTTAAAATTAAAGGTTTGACAGCCGAACAAGAACAAAATGTACGCCAAATCCACGCCATGAACCCGGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCAGAGCTGGATTTAGACAGCTATAAAAAAGCGGCAGATACGCTTTTCGATGAGGGATTTGGCCTCTGTATCCGCTACAACCGGCAAACCTCCATTAAAGATTTT  >  1/855062‑855545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑