Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	855,250	A→T	H176L (CAC→CTC)	unknown →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	855,250	0	A	T	100.0%	161.1 / NA	59	H176L (CAC→CTC)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (39/20);  total (39/20)

TTAATCTGCTTAAAGGTGTGTATAACCCGTCGGCGGCAAATAATCAAACCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCACAATAAGTTTTTCCGACCCGGCAATGTCAGCCCCGGTAATGTGGCAACAGATGAGACTATCCCGGGCTTTCGCGGCATTGTCACTACCGTGTTTTCGGGCCTTATCAGCTGCTATAACGCATACCCTAAAAAGCACAGCTACCGGGTGCGCCGTGCCCACAAAGGCTGGCACAATGGCGTGGTATGGTACGCCGAAAAAGCCAAAATCCTGCTGCGCAATGACAATCTTAAAATTAAAGGTTTGACAGCCGAACAAGAACAAAATGTACGCCAAATCCACGCCATGAACCCGGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCAGAGCTGGATTTAGACAGCTATAAAAAAGCGGCAGATACGCTTTTCGATG  >  1/855004‑855492                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   ttAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCaaa                                                                                                                                                                                                                                                <  1:106708/251‑1 (MQ=255)        gCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAgg                                                                                                                                                                                                                                              >  1:384705/1‑246 (MQ=255)        gCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTgg                                                                                                                                                                                                                                          >  1:51602/1‑250 (MQ=255)        gCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTgg                                                                                                                                                                                                                                          >  1:22678/1‑250 (MQ=255)               ggggtgtATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATgg                                                                                                                                                                                                                                  >  1:819858/4‑251 (MQ=255)                  gtgtATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATgg                                                                                                                                                                                                                                  >  1:759106/1‑248 (MQ=255)                    gtATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGCGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATggggtat                                                                                                                                                                                                                             >  1:612641/1‑246 (MQ=255)                       taACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATggggtatggt                                                                                                                                                                                                                          <  1:430838/251‑9 (MQ=255)                       taACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATggggtatggt                                                                                                                                                                                                                          <  1:826927/251‑9 (MQ=255)                         aCCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATggggtat                                                                                                                                                                                                                             >  1:70541/1‑241 (MQ=255)                         aCCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACACGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATggggtatggtac                                                                                                                                                                                                                        <  1:772783/251‑11 (MQ=255)                                    gcAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGaaaaatg                                                                                                                                                                                                          >  1:149503/1‑249 (MQ=255)                                         taatCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATggggtatggtac                                                                                                                                                                                                                        <  1:845900/235‑11 (MQ=255)                                         taatCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATggggtatggt                                                                                                                                                                                                                          >  1:508408/1‑225 (MQ=255)                                               agccGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGcac                                                                                                                                                                                                                                       >  1:511702/3‑214 (MQ=255)                                                           gcgcAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGAc                                                                                                                                                                                    <  1:30435/250‑1 (MQ=255)                                                              cAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATc                                                                                                                                                                                <  1:865665/251‑1 (MQ=255)                                                              aaggcgcTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTGCCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATc                                                                                                                                                                                <  1:834943/248‑1 (MQ=255)                                                                    cTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGa                                                                                                                                                                                     >  1:841382/1‑240 (MQ=255)                                                                        aaaaaaGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGa                                                                                                                                                                                     <  1:515774/236‑1 (MQ=255)                                                                                      gcataaTAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTaagggggtaacggcag                                                                                                                                                        >  1:436180/5‑237 (MQ=255)                                                                                       cataaTAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTaagggggtaacggc                                                                                                                                                          <  1:681245/245‑13 (MQ=255)                                                                                         taaTAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCaaa                                                                                                                                                                                                                                                <  1:312476/162‑1 (MQ=255)                                                                                         taaTAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTgcgc                                                                                                                                                                                          <  1:826628/213‑1 (MQ=255)                                                                                         taaTAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTaagggggtaacggcagagc                                                                                                                                                     <  1:70762/250‑18 (MQ=255)                                                                                                                                                                    cATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGc                                                                           <  1:705772/250‑1 (MQ=255)                                                                                                                                                                    cATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGc                                                                           <  1:936/250‑1 (MQ=255)                                                                                                                                                                     aTTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATaaa                                                                              <  1:693491/246‑1 (MQ=255)                                                                                                                                                                       tGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGc                                                                           >  1:381427/1‑247 (MQ=255)                                                                                                                                                                       tGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTggg                                                                       >  1:840566/1‑251 (MQ=255)                                                                                                                                                                       tGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTggg                                                                       >  1:39040/1‑251 (MQ=255)                                                                                                                                                                       tGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTggg                                                                       >  1:148821/1‑251 (MQ=255)                                                                                                                                                                            cTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTGCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCTCATATCCTGGTTGAATGCGCCACTAATAAAAGc                                                                           >  1:163371/1‑242 (MQ=255)                                                                                                                                                                            cTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGc                                                                           >  1:582862/1‑242 (MQ=255)                                                                                                                                                                                 gtgtTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCaaaaa                                                             >  1:570486/1‑251 (MQ=255)                                                                                                                                                                                 gtgtTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCaaaaa                                                             >  1:847608/1‑251 (MQ=255)                                                                                                                                                                                     tttCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGa                                                          >  1:316830/1‑250 (MQ=255)                                                                                                                                                                                      ttcaggCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACt                                                        <  1:247708/247‑1 (MQ=255)                                                                                                                                                                                          ggCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTAt                                                     >  1:23902/1‑250 (MQ=255)                                                                                                                                                                                             cTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATccga                                                 >  1:879461/1‑248 (MQ=255)                                                                                                                                                                                                       gctATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTa                                       >  1:11148/1‑251 (MQ=255)                                                                                                                                                                                                        ctATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAg                                      >  1:241348/1‑251 (MQ=255)                                                                                                                                                                                                        ctATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAg                                      >  1:487576/1‑251 (MQ=255)                                                                                                                                                                                                        ctATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAg                                      >  1:510612/1‑251 (MQ=255)                                                                                                                                                                                                        ctATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAAACTTATCCGAGCTGGATTTAg                                      >  1:870074/1‑251 (MQ=255)                                                                                                                                                                                                          ataACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGAc                                    <  1:876568/251‑1 (MQ=255)                                                                                                                                                                                                            aaCGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAg                                  >  1:490477/1‑251 (MQ=255)                                                                                                                                                                                                              cgcttACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGc                                                                           >  1:78965/5‑208 (MQ=255)                                                                                                                                                                                                                    cccTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACaaaa                          >  1:632450/1‑251 (MQ=255)                                                                                                                                                                                                                      cTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACaaaaaa                        >  1:432391/1‑251 (MQ=255)                                                                                                                                                                                                                        aaaaaaCACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATTACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAg                       >  1:724342/1‑250 (MQ=255)                                                                                                                                                                                                                           aaacacaGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCgg                    >  1:410924/4‑250 (MQ=255)                                                                                                                                                                                                                             acacaGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCgg                    >  1:528457/2‑248 (MQ=255)                                                                                                                                                                                                                                  gCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGc                                                                           >  1:107733/1‑188 (MQ=255)                                                                                                                                                                                                                                  gCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGAc                                    >  1:385703/1‑227 (MQ=255)                                                                                                                                                                                                                                       cgtctGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGa                                     <  1:263204/217‑1 (MQ=255)                                                                                                                                                                                                                                       cgtctGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACtttt       >  1:159898/5‑251 (MQ=255)                                                                                                                                                                                                                                        gtctGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACtttt       >  1:193367/4‑250 (MQ=255)                                                                                                                                                                                                                                             cgcCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCCGAGCTGGATTTAGACAGCTACAAAAAAGCGGCTGATACACTTTTCGacg  <  1:80101/250‑3 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   TTAATCTGCTTAAAGGTGTGTATAACCCGTCGGCGGCAAATAATCAAACCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCACAATAAGTTTTTCCGACCCGGCAATGTCAGCCCCGGTAATGTGGCAACAGATGAGACTATCCCGGGCTTTCGCGGCATTGTCACTACCGTGTTTTCGGGCCTTATCAGCTGCTATAACGCATACCCTAAAAAGCACAGCTACCGGGTGCGCCGTGCCCACAAAGGCTGGCACAATGGCGTGGTATGGTACGCCGAAAAAGCCAAAATCCTGCTGCGCAATGACAATCTTAAAATTAAAGGTTTGACAGCCGAACAAGAACAAAATGTACGCCAAATCCACGCCATGAACCCGGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCAGAGCTGGATTTAGACAGCTATAAAAAAGCGGCAGATACGCTTTTCGATG  >  1/855004‑855492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑