Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	855,200	C→T	S159S (AGC→AGT)	unknown →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	855,200	0	C	T	100.0%	143.1 / NA	53	S159S (AGC→AGT)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/26);  total (27/26)

ATCCTGATGGGTGAGCCGGACCAAAAGCCGACCCAAATGCTGATTAATCTGCTTAAAGGTGTGTATAACCCGTCGGCGGCAAATAATCAAACCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCACAATAAGTTTTTCCGACCCGGCAATGTCAGCCCCGGTAATGTGGCAACAGATGAGACTATCCCGGGCTTTCGCGGCATTGTCACTACCGTGTTTTCGGGCCTTATCAGCTGCTATAACGCATACCCTAAAAAGCACAGCTACCGGGTGCGCCGTGCCCACAAAGGCTGGCACAATGGCGTGGTATGGTACGCCGAAAAAGCCAAAATCCTGCTGCGCAATGACAATCTTAAAATTAAAGGTTTGACAGCCGAACAAGAACAAAATGTACGCCAAATCCACGCCATGAACCCGGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCAGA  >  1/854961‑855445                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      attttaaTGGGCGAACCGGACCAAAAGCCGACCCAAATGCTGATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGc                                                                                                                                                                                                                                            <  1:554196/245‑1 (MQ=255) attttaaTGGGCGAACCGGACCAAAAGCCGACCCAAATGCTGATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGc                                                                                                                                                                                                                                            <  1:372824/245‑1 (MQ=255)                 cGGACCAAAAGCCGACCCAAATGCTGATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAacac                                                                                                                                                                                                                            <  1:61062/251‑1 (MQ=255)                   gACCAAAAGCCGACCCAAATGCTGATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGc                                                                                                                                                                                                                                            <  1:678302/233‑1 (MQ=255)                   gACCAAAAGCCGACCCAAATGCTGATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAg                                                                                                                                                                                                                          >  1:513218/1‑251 (MQ=255)                       aaaaGCCGACCCAAATGCTGATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTAc                                                                                                                                                                                                                      <  1:555558/251‑1 (MQ=255)                       aaaaGCCGACCCAAATGCTGATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTAc                                                                                                                                                                                                                      <  1:328909/251‑1 (MQ=255)                         aaGCCGACCCAAATGCTGATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCg                                                                                                                                                                                                                    <  1:431427/251‑1 (MQ=255)                                cccAAATGCTGATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCgtctgcgc                                                                                                                                                                                                             <  1:346796/251‑8 (MQ=255)                                            ttAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCaaa                                                                                                                                                                                                 <  1:106708/251‑1 (MQ=255)                                                   gCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCgtctgcgc                                                                                                                                                                                                             >  1:891950/1‑225 (MQ=255)                                                   gCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAgg                                                                                                                                                                                               >  1:384705/1‑246 (MQ=255)                                                   gCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTgg                                                                                                                                                                                           >  1:22678/1‑250 (MQ=255)                                                   gCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTgg                                                                                                                                                                                           >  1:51602/1‑250 (MQ=255)                                                    cTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGc                                                                                                                                                                                                                                            <  1:394380/200‑1 (MQ=255)                                                          ggggtgtATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATgg                                                                                                                                                                                   >  1:819858/4‑251 (MQ=255)                                                             gtgtATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATgg                                                                                                                                                                                   >  1:759106/1‑248 (MQ=255)                                                               gtATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGGTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCgtc                                                                                                                                                                                                                  >  1:892568/1‑213 (MQ=255)                                                               gtATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGCGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATggggtat                                                                                                                                                                              >  1:612641/1‑246 (MQ=255)                                                                  taACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATggggtatggt                                                                                                                                                                           <  1:430838/251‑9 (MQ=255)                                                                  taACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATggggtatggt                                                                                                                                                                           <  1:826927/251‑9 (MQ=255)                                                                    aCCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATggggtat                                                                                                                                                                              >  1:70541/1‑241 (MQ=255)                                                                    aCCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACACGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATggggtatggtac                                                                                                                                                                         <  1:772783/251‑11 (MQ=255)                                                                            gcggcAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGAATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGCAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTaaacaac                                                                                                                                                                                                                              >  1:770145/1‑186 (MQ=255)                                                                               gcAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGaaaaatg                                                                                                                                                           >  1:149503/1‑249 (MQ=255)                                                                                    taatCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATggggtatggtac                                                                                                                                                                         <  1:845900/235‑11 (MQ=255)                                                                                    taatCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATggggtatggt                                                                                                                                                                           >  1:508408/1‑225 (MQ=255)                                                                                          agccGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGcac                                                                                                                                                                                        >  1:511702/3‑214 (MQ=255)                                                                                                      gcgcAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGAc                                                                                                                                     <  1:30435/250‑1 (MQ=255)                                                                                                         cAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATc                                                                                                                                 <  1:865665/251‑1 (MQ=255)                                                                                                         aaggcgcTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTGCCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATc                                                                                                                                 <  1:834943/248‑1 (MQ=255)                                                                                                               cTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGa                                                                                                                                      >  1:841382/1‑240 (MQ=255)                                                                                                                   aaaaaaGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGa                                                                                                                                      <  1:515774/236‑1 (MQ=255)                                                                                                                                 gcataaTAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTaagggggtaacggcag                                                                                                         >  1:436180/5‑237 (MQ=255)                                                                                                                                  cataaTAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTaagggggtaacggc                                                                                                           <  1:681245/245‑13 (MQ=255)                                                                                                                                    taaTAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCaaa                                                                                                                                                                                                 <  1:312476/162‑1 (MQ=255)                                                                                                                                    taaTAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTgcgc                                                                                                                                           <  1:826628/213‑1 (MQ=255)                                                                                                                                    taaTAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTaagggggtaacggcagagc                                                                                                      <  1:70762/250‑18 (MQ=255)                                                                                                                                                                                                               cATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGc                            <  1:936/250‑1 (MQ=255)                                                                                                                                                                                                               cATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGc                            <  1:705772/250‑1 (MQ=255)                                                                                                                                                                                                                aTTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATaaa                               <  1:693491/246‑1 (MQ=255)                                                                                                                                                                                                                  tGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGc                            >  1:381427/1‑247 (MQ=255)                                                                                                                                                                                                                  tGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTggg                        >  1:39040/1‑251 (MQ=255)                                                                                                                                                                                                                  tGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTggg                        >  1:840566/1‑251 (MQ=255)                                                                                                                                                                                                                  tGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTggg                        >  1:148821/1‑251 (MQ=255)                                                                                                                                                                                                                       cTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTGCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCTCATATCCTGGTTGAATGCGCCACTAATAAAAGc                            >  1:163371/1‑242 (MQ=255)                                                                                                                                                                                                                       cTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGc                            >  1:582862/1‑242 (MQ=255)                                                                                                                                                                                                                            gtgtTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCaaaaa              >  1:570486/1‑251 (MQ=255)                                                                                                                                                                                                                            gtgtTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCaaaaa              >  1:847608/1‑251 (MQ=255)                                                                                                                                                                                                                                tttCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGa           >  1:316830/1‑250 (MQ=255)                                                                                                                                                                                                                                 ttcaggCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACt         <  1:247708/247‑1 (MQ=255)                                                                                                                                                                                                                                     ggCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTAt      >  1:23902/1‑250 (MQ=255)                                                                                                                                                                                                                                        cTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATccga  >  1:879461/1‑248 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      ATCCTGATGGGTGAGCCGGACCAAAAGCCGACCCAAATGCTGATTAATCTGCTTAAAGGTGTGTATAACCCGTCGGCGGCAAATAATCAAACCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCACAATAAGTTTTTCCGACCCGGCAATGTCAGCCCCGGTAATGTGGCAACAGATGAGACTATCCCGGGCTTTCGCGGCATTGTCACTACCGTGTTTTCGGGCCTTATCAGCTGCTATAACGCATACCCTAAAAAGCACAGCTACCGGGTGCGCCGTGCCCACAAAGGCTGGCACAATGGCGTGGTATGGTACGCCGAAAAAGCCAAAATCCTGCTGCGCAATGACAATCTTAAAATTAAAGGTTTGACAGCCGAACAAGAACAAAATGTACGCCAAATCCACGCCATGAACCCGGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGGGCGGCAAAAAAGACTTATCAGA  >  1/854961‑855445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑