Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	855,179	C→T	T152T (ACC→ACT)	unknown →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	855,179	0	C	T	100.0%	152.6 / NA	58	T152T (ACC→ACT)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/29);  total (29/29)

GTGAGGGAGGCATCCAGGGGCGAATGGAAATCCTGATGGGTGAGCCGGACCAAAAGCCGACCCAAATGCTGATTAATCTGCTTAAAGGTGTGTATAACCCGTCGGCGGCAAATAATCAAACCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCACAATAAGTTTTTCCGACCCGGCAATGTCAGCCCCGGTAATGTGGCAACAGATGAGACTATCCCGGGCTTTCGCGGCATTGTCACTACCGTGTTTTCGGGCCTTATCAGCTGCTATAACGCATACCCTAAAAAGCACAGCTACCGGGTGCGCCGTGCCCACAAAGGCTGGCACAATGGCGTGGTATGGTACGCCGAAAAAGCCAAAATCCTGCTGCGCAATGACAATCTTAAAATTAAAGGTTTGACAGCCGAACAAGAACAAAATGTACGCCAAATCCACGCCATGAACCCGGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGG  >  1/854932‑855423                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     gTGAGGGCGGCATCCAGGGTCGTATGGAGATTTTAATGGGCGAACCGGACCAAAAGCCGACCCAAGTGCTGATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACtgtg                                                                                                                                                                                                                                                   >  1:653993/1‑251 (MQ=255)  tGAGGGCGGCATCCAGGGTCGTATGGAGATTTTAATGGGCGAACCGGACCAAAAGCCGACCCAAATGCTGATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTgtgt                                                                                                                                                                                                                                                  <  1:729631/251‑1 (MQ=255)       gcggCATCCAGGGTCGTATGGAGATTTTAATGGGCGAACCGGACCAAAAGCCGACCCAAATGCTGATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTc                                                                                                                                                                                                                                              <  1:580600/248‑1 (MQ=255)        cggCATCCAGGGTCGTATGGAGATTTTAATGGGCGAACCGGACCAAAAGCCGACCCAAATGCTGATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTcag                                                                                                                                                                                                                                            <  1:689312/250‑3 (MQ=255)            atccagggtcgtatggagattttaaTGGGCGAACCGGACCAAAAGCCGACCCAAATGCTGATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCTGCAATGTCAGCCCCGGCAGCGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGc                                                                                                                                                                                                                                          >  1:341952/25‑249 (MQ=255)            atccagggtcgtatggagattttaaTGGGCGAACCGGACCAAAAGCCGACCCAAATGCTGATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGc                                                                                                                                                                                                                                          >  1:28247/25‑249 (MQ=255)            atccagggtcgtatggagattttaaTGGGCGAACCGGACCAAAAGCCGACCCAAATGCTGATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGc                                                                                                                                                                                                                                          >  1:872816/25‑249 (MQ=255)            atccagggtcgtatggagattttaaTGGGCGAACCGGACCAAAAGCCGACCCAAATGCTGATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGc                                                                                                                                                                                                                                          >  1:580596/25‑249 (MQ=255)                   gtcgtatggagattttaaTGGGCGAACCGGACCAAAAGCCGACCCAAATGCTGATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAgt                                                                                                                                                                                                                                 >  1:600546/18‑250 (MQ=255)                   gtcgtatggagattttaaTGGGCGAACCGGACCAAAAGCCGACCCAAATGCTGATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAg                                                                                                                                                                                                                                  <  1:56549/233‑1 (MQ=255)                      gtatggagatttcaaTGGGCGAACCGGACCAAAAGCCGACCCAAATGCTGATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAgttg                                                                                                                                                                                                                               >  1:470632/15‑247 (MQ=255)                              attttaaTGGGCGAACCGGACCAAAAGCCGACCCAAATGCTGATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGc                                                                                                                                                                                                                      <  1:372824/245‑1 (MQ=255)                              attttaaTGGGCGAACCGGACCAAAAGCCGACCCAAATGCTGATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGc                                                                                                                                                                                                                      <  1:554196/245‑1 (MQ=255)                                              cGGACCAAAAGCCGACCCAAATGCTGATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAacac                                                                                                                                                                                                      <  1:61062/251‑1 (MQ=255)                                                gACCAAAAGCCGACCCAAATGCTGATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGc                                                                                                                                                                                                                      <  1:678302/233‑1 (MQ=255)                                                gACCAAAAGCCGACCCAAATGCTGATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAg                                                                                                                                                                                                    >  1:513218/1‑251 (MQ=255)                                                    aaaaGCCGACCCAAATGCTGATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTAc                                                                                                                                                                                                <  1:328909/251‑1 (MQ=255)                                                    aaaaGCCGACCCAAATGCTGATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTAc                                                                                                                                                                                                <  1:555558/251‑1 (MQ=255)                                                      aaGCCGACCCAAATGCTGATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCg                                                                                                                                                                                              <  1:431427/251‑1 (MQ=255)                                                             cccAAATGCTGATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCgtctgcgc                                                                                                                                                                                       <  1:346796/251‑8 (MQ=255)                                                                       gATTAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGcc                                                                                                                                                                                                                                         >  1:577690/1‑191 (MQ=255)                                                                         ttAATCTGCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCaaa                                                                                                                                                                           <  1:106708/251‑1 (MQ=255)                                                                                gCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCgtctgcgc                                                                                                                                                                                       >  1:891950/1‑225 (MQ=255)                                                                                gCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAgg                                                                                                                                                                         >  1:384705/1‑246 (MQ=255)                                                                                gCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTgg                                                                                                                                                                     >  1:22678/1‑250 (MQ=255)                                                                                gCTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTgg                                                                                                                                                                     >  1:51602/1‑250 (MQ=255)                                                                                 cTTAAAGGGGTGTATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGc                                                                                                                                                                                                                      <  1:394380/200‑1 (MQ=255)                                                                                       ggggtgtATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATgg                                                                                                                                                             >  1:819858/4‑251 (MQ=255)                                                                                          gtgtATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATgg                                                                                                                                                             >  1:759106/1‑248 (MQ=255)                                                                                            gtATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGGTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCgtc                                                                                                                                                                                            >  1:892568/1‑213 (MQ=255)                                                                                            gtATAACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGCGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATggggtat                                                                                                                                                        >  1:612641/1‑246 (MQ=255)                                                                                               taACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATggggtatggt                                                                                                                                                     <  1:430838/251‑9 (MQ=255)                                                                                               taACCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATggggtatggt                                                                                                                                                     <  1:826927/251‑9 (MQ=255)                                                                                                 aCCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATggggtat                                                                                                                                                        >  1:70541/1‑241 (MQ=255)                                                                                                 aCCCGTCGGCGGCAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACACGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATggggtatggtac                                                                                                                                                   <  1:772783/251‑11 (MQ=255)                                                                                                         gcggcAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGAATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGCAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTaaacaac                                                                                                                                                                                                        >  1:770145/1‑186 (MQ=255)                                                                                                            gcAAATAATCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGaaaaatg                                                                                                                                     >  1:149503/1‑249 (MQ=255)                                                                                                                 taatCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATggggtatggtac                                                                                                                                                   <  1:845900/235‑11 (MQ=255)                                                                                                                 taatCAAGCCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATggggtatggt                                                                                                                                                     >  1:508408/1‑225 (MQ=255)                                                                                                                       agccGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGcac                                                                                                                                                                  >  1:511702/3‑214 (MQ=255)                                                                                                                                   gcgcAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGAc                                                                                                               <  1:30435/250‑1 (MQ=255)                                                                                                                                      cAAGCGCTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATc                                                                                                           <  1:865665/251‑1 (MQ=255)                                                                                                                                      aaggcgcTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTGCCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATc                                                                                                           <  1:834943/248‑1 (MQ=255)                                                                                                                                            cTTTAAAAAAGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGa                                                                                                                >  1:841382/1‑240 (MQ=255)                                                                                                                                                aaaaaaGACGGGCAGCATAATAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGa                                                                                                                <  1:515774/236‑1 (MQ=255)                                                                                                                                                              gcataaTAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTaagggggtaacggcag                                                                                   >  1:436180/5‑237 (MQ=255)                                                                                                                                                               cataaTAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTaagggggtaacggc                                                                                     <  1:681245/245‑13 (MQ=255)                                                                                                                                                                 taaTAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCaaa                                                                                                                                                                           <  1:312476/162‑1 (MQ=255)                                                                                                                                                                 taaTAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTgcgc                                                                                                                     <  1:826628/213‑1 (MQ=255)                                                                                                                                                                 taaTAAATTTTTCCGACCCGGCAATGTCAGCCCCGGCAGTGTAGCAACAGATGAGACTATCCCCGGCTTTCGTGGCATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTaagggggtaacggcagagc                                                                                <  1:70762/250‑18 (MQ=255)                                                                                                                                                                                                                                            cATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGc      <  1:936/250‑1 (MQ=255)                                                                                                                                                                                                                                            cATTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGc      <  1:705772/250‑1 (MQ=255)                                                                                                                                                                                                                                             aTTGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATaaa         <  1:693491/246‑1 (MQ=255)                                                                                                                                                                                                                                               tGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGc      >  1:381427/1‑247 (MQ=255)                                                                                                                                                                                                                                               tGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTggg  >  1:840566/1‑251 (MQ=255)                                                                                                                                                                                                                                               tGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTggg  >  1:39040/1‑251 (MQ=255)                                                                                                                                                                                                                                               tGTCACTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTggg  >  1:148821/1‑251 (MQ=255)                                                                                                                                                                                                                                                    cTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTGCCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCTCATATCCTGGTTGAATGCGCCACTAATAAAAGc      >  1:163371/1‑242 (MQ=255)                                                                                                                                                                                                                                                    cTACTGTGTTTTCAGGCCTTATCAGTTGCTATAACGCTTACCCTAAAAAACACAGCTACCGTCTGCGCCGTGCCCTCAAAGGCTGGCACAATGG‑‑‑GGTATGGTACCCCGAAAAATGCAAAATCGTGCTGCGCAATGACAATCTCAAAATTAAGGGGGTAACGGCAGAGCAGGAAGAAAATTTACGTGAGATCCACGCGATGAACCCCGCACATATCCTGGTTGAATGCGCCACTAATAAAAGc      >  1:582862/1‑242 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     GTGAGGGAGGCATCCAGGGGCGAATGGAAATCCTGATGGGTGAGCCGGACCAAAAGCCGACCCAAATGCTGATTAATCTGCTTAAAGGTGTGTATAACCCGTCGGCGGCAAATAATCAAACCGGGCGCGGGCGCAAGCGCTTTAAAAAAGACGGGCAGCACAATAAGTTTTTCCGACCCGGCAATGTCAGCCCCGGTAATGTGGCAACAGATGAGACTATCCCGGGCTTTCGCGGCATTGTCACTACCGTGTTTTCGGGCCTTATCAGCTGCTATAACGCATACCCTAAAAAGCACAGCTACCGGGTGCGCCGTGCCCACAAAGGCTGGCACAATGGCGTGGTATGGTACGCCGAAAAAGCCAAAATCCTGCTGCGCAATGACAATCTTAAAATTAAAGGTTTGACAGCCGAACAAGAACAAAATGTACGCCAAATCCACGCCATGAACCCGGCACATATCCTGGTTGAATGCGCCACTAATAAAAGCTGGG  >  1/854932‑855423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑