Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	854,013	G→A	A30A (GCG→GCA)	unknown →	Phage FAD/FMN‑containing dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	854,013	0	G	A	100.0%	187.3 / NA	69	A30A (GCG→GCA)	unknown	Phage FAD/FMN‑containing dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/34);  total (35/34)

AAGCAAGACGTTCCTTCCCCATATACGCTTTACGATACCGATTGCAAAGTCAAGCCCGGCAACTTTGTGGTGGCGGGGCTTACAATCAGCGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGCTGGTTTAATGCGGGTTTTATCGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGCGCGGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACGCAAAAGCTTTCAATCGGCACGGTCATTAAAGTCTATCCCGGTTGTGACGGACGGGCACAAACCTGTCTTAAAAAATTTAACAATATGCTTAATTTCGGCGGTATTCCACATATGCCGACCAAATCGCCGTATGACGGTTCACGCGTGTTTTAGA  >  1/853858‑854257                                                                                                                                                            |                                                                                                                                                                                                                                                     acctgggggcgtaattgtccgtataCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACaaa                                                                                                                                                        <  1:134146/229‑1 (MQ=255)    cccgtccgtcacagcccgtataCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGc                                                                                                                                                     <  1:598881/232‑1 (MQ=255)       gggcgtaattgtccgtataCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAg                                                                                                                                                      >  1:246871/16‑246 (MQ=255)         gcgtaattgtccgtataCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCAc                                                                                                                                                                                                    <  1:886884/185‑1 (MQ=255)            taattgtccgtataCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTgcgggca                                                                                                                                                                                                         <  1:66382/180‑5 (MQ=255)                    cgtataCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCAccgta                                                                                                                                    >  1:875676/3‑247 (MQ=255)                     gtataCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCAccgtaa                                                                                                                                   >  1:518966/2‑246 (MQ=255)                       ataCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCAccgtaatt                                                                                                                                 <  1:671708/251‑8 (MQ=255)                         aCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTaa                                                                                                                               <  1:127807/251‑1 (MQ=255)                         aCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTaa                                                                                                                               <  1:879014/251‑1 (MQ=255)                          cGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAAt                                                                                                                                                                      >  1:403550/1‑211 (MQ=255)                          cGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTaaa                                                                                                                              >  1:856852/1‑251 (MQ=255)                            cTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCAcac                                                                                                                                                           <  1:20524/220‑3 (MQ=255)                            cTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTa                                                                                                                                >  1:405102/1‑247 (MQ=255)                              ttatgATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGGTGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAgc                                                                                                                            >  1:600062/5‑248 (MQ=255)                              ttatgATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTAttg                                                                                                                                                                                                                                            <  1:271276/133‑3 (MQ=255)                              ttatgATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTAt                                                                                                                                                                                                                                              >  1:421825/5‑135 (MQ=255)                              ttatgATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATggg                                                                                                                                                                   >  1:755293/5‑210 (MQ=255)                              ttatgATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGt                                                                                                                            >  1:729176/5‑249 (MQ=255)                              ttatgATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGt                                                                                                                            >  1:649664/5‑249 (MQ=255)                              ttatgATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGt                                                                                                                            >  1:902005/5‑249 (MQ=255)                                      ccGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATccgggc                                                                                                                  <  1:716353/251‑5 (MQ=255)                                          ttGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACaaa                                                                                                                                                        <  1:26111/209‑1 (MQ=255)                                          ttGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCtgtg                                                                                                              >  1:556426/1‑251 (MQ=255)                                                ggttaaGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCAc                                                                                                                                                                                                    >  1:108953/5‑159 (MQ=255)                                                ggttaaGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGc                                                                                                                                                     <  1:393205/202‑1 (MQ=255)                                                 gttaaGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTgacggacg                                                                                                       >  1:47607/4‑251 (MQ=255)                                                       cccGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGcgc                                                                                                  <  1:353701/250‑3 (MQ=255)                                                           gCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAAc                                                                                              <  1:516917/250‑1 (MQ=255)                                                                             ggCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTAttg                                                                                                                                                                                                                                            >  1:323747/1‑88 (MQ=255)                                                                             ggCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATccgggc                                                                                                                  >  1:848197/1‑208 (MQ=255)                                                                              gCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATccggacaattacgccccc                                                                                                      <  1:739306/223‑17 (MQ=255)                                                                              gCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAAc                                                                           <  1:533816/250‑1 (MQ=255)                                                                              gCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAACa                                                                          >  1:39332/1‑251 (MQ=255)                                                                              gCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAACa                                                                          >  1:871529/1‑251 (MQ=255)                                                                               cTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAg                                                                                                                             <  1:406400/199‑1 (MQ=255)                                                                                 tACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATcc                                                                                                                      >  1:166489/1‑204 (MQ=255)                                                                                   caattagtgcgATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATg                                                                                                                                                                     >  1:496350/9‑155 (MQ=255)                                                                                   caattagtgcgATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATg                                                                                                                                                                     <  1:726503/147‑1 (MQ=255)                                                                                   caattagtgcgATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATccgggc                                                                                                                  <  1:488406/198‑5 (MQ=255)                                                                                   caattagtgcgATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAACAATATg                                                                     >  1:160908/9‑251 (MQ=255)                                                                                         gtgcgATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAACAATATGCTTAAt                                                               >  1:894604/3‑251 (MQ=255)                                                                                                ggACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAACAATATGCTTAATTTcggcg                                                        >  1:27817/1‑251 (MQ=255)                                                                                                 gACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAACAATATGCTTAATTTcggcgg                                                       >  1:713664/1‑251 (MQ=255)                                                                                                 gACGGGGCGTCCATCACCGTAAATCTGCCGGC‑AACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAACAATATGCTTa                                                                 >  1:312941/1‑240 (MQ=255)                                                                                                     gggCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTgac                                                                                                            >  1:457492/1‑194 (MQ=255)                                                                                                       gCGTCCATCGCCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAACAATATGCTTAATTTCGGCGGTATTc                                                  <  1:304945/250‑1 (MQ=255)                                                                                                         gTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATggg                                                                                                                                                                   >  1:821724/1‑135 (MQ=255)                                                                                                         gTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAACaa                                                                         <  1:680932/225‑1 (MQ=255)                                                                                                          tCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAACAATATGCTTAATTTCGGCGGTATTccgc                                               <  1:206296/250‑3 (MQ=255)                                                                                                           ccATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAACAATATGCTTAATTTCGGCGGTATTccgcac                                             <  1:89418/251‑5 (MQ=255)                                                                                                           ccATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAACAATATGCTTAATTTCGGCGGTATTccgcac                                             <  1:512153/251‑5 (MQ=255)                                                                                                           ccATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAACAATATGCTTAATTTCGGCGGTATTccgcac                                             <  1:775486/251‑5 (MQ=255)                                                                                                           ccATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAACAATATGCTTAATTTCGGCGGTATTccgcac                                             <  1:785450/251‑5 (MQ=255)                                                                                                              tCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAACAATATGCTTAATTTCGGCGGTATTccgcacatg                                          >  1:678237/1‑244 (MQ=255)                                                                                                                 ccGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAAcc                                                                                             >  1:497276/1‑197 (MQ=255)                                                                                                                 ccGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAAcc                                                                                             <  1:585491/197‑1 (MQ=255)                                                                                                                 ccGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAACAATATGCTTAAt                                                               >  1:562887/1‑227 (MQ=255)                                                                                                                 ccGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAACAATATGCTTAATTTCGGCGGTATTCCGCACATGcca                                       >  1:874022/1‑250 (MQ=255)                                                                                                                     aaaTCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAACAATATGCTTAATTTCGGCGGTATTCCGCACATGCCAACCa                                   <  1:81734/251‑1 (MQ=255)                                                                                                                                   aCCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAACAATATGCTTAATTTCGGCGGTATTCCGCACATGCCAACCAAATCGCCGTATGAc                     <  1:375749/251‑1 (MQ=255)                                                                                                                                   aCCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAACAATATGCTTAATTTCGGCGGTATTCCGCACATGCCAACCAAATCGCCGTATGAc                     <  1:480816/251‑1 (MQ=255)                                                                                                                                     cTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAACAATATGCTTAATTTCGGCGGTATTCCGCACATGCCAACCAAATCGCCGTATGACgg                   <  1:217047/251‑1 (MQ=255)                                                                                                                                              ggttGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAg                                                                                                                             <  1:292106/133‑1 (MQ=255)                                                                                                                                              ggttGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAACAATATGCTTAATTTCGGCGGTATTCCGCACATGCCAACCAAATCGCCGTATGa                      <  1:532475/236‑1 (MQ=255)                                                                                                                                              ggttGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAACAATATGCTTAATTTCGGCGGTATTCCGCACATGCCAACCAAATCGCCGTATGACGGTTCACGCgt          >  1:223269/4‑251 (MQ=255)                                                                                                                                               gttGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAACAATATGCTTAATTTCGGCGGTATTCCGCACATGCCAACCAAATCGCCGTATGACGGTTCACGCgtg         >  1:819735/3‑251 (MQ=255)                                                                                                                                                   gTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAACAATATGCTTAATTTCGGCGGTATTccgcac                                             >  1:700383/1‑207 (MQ=255)                                                                                                                                                      tAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAACAATATGCTTAATTTCGGCGGTATTCCGCACATGCCAACCAAATCGCCGTATGACGGTTCACGCGTGTTTTagg  <  1:724921/251‑2 (MQ=255)                                                                                                                                                            |                                                                                                                                                                                                                                                     AAGCAAGACGTTCCTTCCCCATATACGCTTTACGATACCGATTGCAAAGTCAAGCCCGGCAACTTTGTGGTGGCGGGGCTTACAATCAGCGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGCTGGTTTAATGCGGGTTTTATCGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGCGCGGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACGCAAAAGCTTTCAATCGGCACGGTCATTAAAGTCTATCCCGGTTGTGACGGACGGGCACAAACCTGTCTTAAAAAATTTAACAATATGCTTAATTTCGGCGGTATTCCACATATGCCGACCAAATCGCCGTATGACGGTTCACGCGTGTTTTAGA  >  1/853858‑854257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑