Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	853,944	C→T	I7I (ATC→ATT)	unknown →	Phage FAD/FMN‑containing dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	853,944	0	C	T	100.0%	102.5 / NA	38	I7I (ATC→ATT)	unknown	Phage FAD/FMN‑containing dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/17);  total (21/17)

AAGCAAGACGTTCCTTCCCCATATACGCTTTACGATACCGATTGCAAAGTCAAGCCCGGCAACTTTGTGGTGGCGGGGCTTACAATCAGCGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGCTGGTTTAATGCGGGTTTTATCGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGCGCGGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACGCAAAAGCTTTCAATCGGCACGGTCATTAAAGTCTATCCCGGTTGTGACGGACGGGCACAAACCTGTCTTAAAAAATTTAACA  >  1/853858‑854185                                                                                       |                                                                                                                                                                                                                                                  acctgggggcgtaattgtccgtataCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACaaa                                                                                <  1:134146/229‑1 (MQ=255)    cccgtccgtcacagcccgtataCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGc                                                                             <  1:598881/232‑1 (MQ=255)       gggcgtaattgtccgtataCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAg                                                                              >  1:246871/16‑246 (MQ=255)         gcgtaattgtccgtataCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCAc                                                                                                                            <  1:886884/185‑1 (MQ=255)           gtaattgtccgtataCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGttt                                                                                                                                                                                    >  1:819804/12‑140 (MQ=255)            taattgtccgtataCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTgcgggca                                                                                                                                 <  1:66382/180‑5 (MQ=255)                    cgtataCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCAccgta                                                            >  1:875676/3‑247 (MQ=255)                     gtataCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCAccgtaa                                                           >  1:518966/2‑246 (MQ=255)                       ataCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCAccgtaatt                                                         <  1:671708/251‑8 (MQ=255)                         aCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTaa                                                       <  1:127807/251‑1 (MQ=255)                         aCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTaa                                                       <  1:879014/251‑1 (MQ=255)                          cGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAAt                                                                                              >  1:403550/1‑211 (MQ=255)                          cGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTaaa                                                      >  1:856852/1‑251 (MQ=255)                            cTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCAcac                                                                                   <  1:20524/220‑3 (MQ=255)                            cTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTa                                                        >  1:405102/1‑247 (MQ=255)                              ttatgATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGGTGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAgc                                                    >  1:600062/5‑248 (MQ=255)                              ttatgATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTAttg                                                                                                                                                                    <  1:271276/133‑3 (MQ=255)                              ttatgATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTAt                                                                                                                                                                      >  1:421825/5‑135 (MQ=255)                              ttatgATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATggg                                                                                           >  1:755293/5‑210 (MQ=255)                              ttatgATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGt                                                    >  1:729176/5‑249 (MQ=255)                              ttatgATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGt                                                    >  1:649664/5‑249 (MQ=255)                              ttatgATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGt                                                    >  1:902005/5‑249 (MQ=255)                                      ccGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATccgggc                                          <  1:716353/251‑5 (MQ=255)                                          ttGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACaaa                                                                                <  1:26111/209‑1 (MQ=255)                                          ttGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCtgtg                                      >  1:556426/1‑251 (MQ=255)                                                ggttaaGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCAc                                                                                                                            >  1:108953/5‑159 (MQ=255)                                                ggttaaGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGc                                                                             <  1:393205/202‑1 (MQ=255)                                                 gttaaGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTgacggacg                               >  1:47607/4‑251 (MQ=255)                                                       cccGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGcgc                          <  1:353701/250‑3 (MQ=255)                                                           gCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAAc                      <  1:516917/250‑1 (MQ=255)                                                                             ggCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTAttg                                                                                                                                                                    >  1:323747/1‑88 (MQ=255)                                                                             ggCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATccgggc                                          >  1:848197/1‑208 (MQ=255)                                                                              gCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATccggacaattacgccccc                              <  1:739306/223‑17 (MQ=255)                                                                              gCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAAc   <  1:533816/250‑1 (MQ=255)                                                                              gCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAACa  >  1:39332/1‑251 (MQ=255)                                                                              gCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCTGTGACGGACGGGCGCAAACCTGTCTTAAAAAATTTAACa  >  1:871529/1‑251 (MQ=255)                                                                               cTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAg                                                     <  1:406400/199‑1 (MQ=255)                                                                                 tACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATcc                                              >  1:166489/1‑204 (MQ=255)                                                                                       |                                                                                                                                                                                                                                                  AAGCAAGACGTTCCTTCCCCATATACGCTTTACGATACCGATTGCAAAGTCAAGCCCGGCAACTTTGTGGTGGCGGGGCTTACAATCAGCGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGCTGGTTTAATGCGGGTTTTATCGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGCGCGGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACGCAAAAGCTTTCAATCGGCACGGTCATTAAAGTCTATCCCGGTTGTGACGGACGGGCACAAACCTGTCTTAAAAAATTTAACA  >  1/853858‑854185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑