Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	853,908	C→T	intergenic (+118/‑16)	unknown → / → unknown	hypothetical protein/Phage FAD/FMN‑containing dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	853,908	0	C	T	100.0%	65.8 / NA	25	intergenic (+118/‑16)	unknown/unknown	hypothetical protein/Phage FAD/FMN‑containing dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/11);  total (14/11)

AAGCAAGACGTTCCTTCCCCATATACGCTTTACGATACCGATTGCAAAGTCAAGCCCGGCAACTTTGTGGTGGCGGGGCTTACAATCAGCGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGCTGGTTTAATGCGGGTTTTATCGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGCGCGGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACGCAAAAGCTTTCAATCGGCACGGTCATTAAAGTCTATCCCGGTTGTG  >  1/853858‑854149                                                   |                                                                                                                                                                                                                                                  acctgggggcgtaattgtccgtataCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACaaa                                            <  1:134146/229‑1 (MQ=255)    cccgtccgtcacagcccgtataCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGc                                         <  1:598881/232‑1 (MQ=255)       gggcgtaattgtccgtataCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAg                                          >  1:246871/16‑246 (MQ=255)         gcgtaattgtccgtataCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCAc                                                                                        <  1:886884/185‑1 (MQ=255)           gtaattgtccgtataCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGttt                                                                                                                                                >  1:819804/12‑140 (MQ=255)            taattgtccgtataCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTgcgggca                                                                                             <  1:66382/180‑5 (MQ=255)                    cgtataCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCAccgta                        >  1:875676/3‑247 (MQ=255)                     gtataCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCAccgtaa                       >  1:518966/2‑246 (MQ=255)                       ataCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCAccgtaatt                     <  1:671708/251‑8 (MQ=255)                         aCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTaa                   <  1:879014/251‑1 (MQ=255)                         aCGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTaa                   <  1:127807/251‑1 (MQ=255)                          cGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAAt                                                          >  1:403550/1‑211 (MQ=255)                          cGCTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTaaa                  >  1:856852/1‑251 (MQ=255)                            cTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCAcac                                               <  1:20524/220‑3 (MQ=255)                            cTTTATGATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTa                    >  1:405102/1‑247 (MQ=255)                              ttatgATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGGTGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAgc                >  1:600062/5‑248 (MQ=255)                              ttatgATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTAttg                                                                                                                                <  1:271276/133‑3 (MQ=255)                              ttatgATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTAt                                                                                                                                  >  1:421825/5‑135 (MQ=255)                              ttatgATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATggg                                                       >  1:755293/5‑210 (MQ=255)                              ttatgATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGt                >  1:729176/5‑249 (MQ=255)                              ttatgATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGt                >  1:649664/5‑249 (MQ=255)                              ttatgATACCGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGt                >  1:902005/5‑249 (MQ=255)                                      ccGATTGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATccgggc      <  1:716353/251‑5 (MQ=255)                                          ttGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACaaa                                            <  1:26111/209‑1 (MQ=255)                                          ttGCAAGGTTAAGCCCGGCAATTTTGTGGTGGCGGGGCTTACAATTAGTGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGTTGGTTTAATGCAGGTTTTATTGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGGGCAGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACACAAAAGCTTGCAATCGGCACCGTAATTAAAGTCTATCCGGGCtgtg  >  1:556426/1‑251 (MQ=255)                                                   |                                                                                                                                                                                                                                                  AAGCAAGACGTTCCTTCCCCATATACGCTTTACGATACCGATTGCAAAGTCAAGCCCGGCAACTTTGTGGTGGCGGGGCTTACAATCAGCGCGATGGACGGGGCGTCCATCACCGTAAATCTGCCGGCAAACCTGCCGCAAGGCTGGTTTAATGCGGGTTTTATCGAGTGGCAGGATGACGGCGTGCGCGAGGTGCGCGCGGTCACCGTGCATCAAAATAACAAGCTGACATTAATGGGTGGCACGCAAAAGCTTTCAATCGGCACGGTCATTAAAGTCTATCCCGGTTGTG  >  1/853858‑854149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑