Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	800,237	T→G	K25T (AAA→ACA)	unknown ←	Peptide chain release factor 2 unshifted fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	800,237	0	T	G	100.0%	213.0 / NA	75	K25T (AAA→ACA)	unknown	Peptide chain release factor 2 unshifted fragment
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (37/38);  total (37/38)

GTTCCAGATTTTTAATGGTGTTGACCACCAGTTCTAAAGAAACGCGTTCTTTGCCGAGCGCCTGCGCTTTCTCTGCATCGTTCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATTTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCACTTATTTAGGAACAACAGAAATGAAAAAAATTATTACTACGCTTTCTCTTTTGGCTATATCTATGAGTGTAACGGCAAATGATGTTGCACTGAAAAGTAAATTGGAA  >  1/800003‑800480                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                    gTTCCAGATTTTTAATGGTGTTGACCACCAGTTCTAAAGAAACGCGTTCTTTGCCGAGCGCCTGCGCTTTCTCTGCATCGTTCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAAt                                                                                                                                                                                                                                     >  1:227017/1‑251 (MQ=255) gTTCCAGATTTTTAATGGTGTTGACCACCAGTTCTAAAGAAACGCGTTCTTTGCCGAGCGCCTGCGCTTTCTCTGCATCGTTCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAAt                                                                                                                                                                                                                                     >  1:834813/1‑251 (MQ=255) gTTCCAGATTTTTAATGGTGTTGACCACCAGTTCTAAAGAAACGCGTTCTTTGCCGAGCGCCTGCGCTTTCTCTGCATCGTTCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAAt                                                                                                                                                                                                                                     >  1:220570/1‑251 (MQ=255)  ttCCAGATTTTTAATGGTGTTGACCACCAGTTCTAAAGAAACGCGTTCTTTGCCGAGCGCCTGCGCTTTCTGTGCATCGTTCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAAt                                                                                                                                                                                                                                     <  1:169910/250‑1 (MQ=255)       gATTTTTAATGGTGTTGACCACCAGTTCTAAAGAAACGCGTTCTTTGCCGAGCGCCTGCGCTTTCTCTGCATCGTTCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTg                                                                                                                                                                                                                               >  1:152905/1‑251 (MQ=255)        aTTTTTAATGGTGTTGACCACCAGTTCTAAAGAAACGCGTTCTTTGCCGAGCGCCTGCGCTTTCTCTGCATCGTTCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTg                                                                                                                                                                                                                               <  1:899358/250‑1 (MQ=255)          ttttAATGGTGTTGACCACCAGTTCTAAAGAAACGCGTTCTTTGCCGAGCGCCTGCGCTTTCTCTGCATCGTTCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCgg                                                                                                                                                                                                                            >  1:760371/1‑251 (MQ=255)                  gtgtTGACCACCAGTTCTAAAGAAACGCGTTCTTTGCCGAGCGCCTGCGCTTTCTCTGCATCGTTCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATaa                                                                                                                                                                                                                    >  1:73924/1‑251 (MQ=255)                  gtgtTGACCACCAGTTCTAAAGAAACGCGTTCTTTGCCGAGCGCCTGCGCTTTCTCTGCATCGTTCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATaa                                                                                                                                                                                                                    >  1:612525/1‑251 (MQ=255)                   tgtTGACCACCAGTTCTAAAGAAACGCGTTCTTTGCCGAGCGCCTGCGCTTTCTCTGCATCGTTCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATaa                                                                                                                                                                                                                    <  1:751942/250‑1 (MQ=255)                     ttGACCACCAGTTCTAAAGAAACGCGTTCTTTGCCGAGCGCCTGCGCTTTCTCTGCATCGTTCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAggg                                                                                                                                                                                                                 <  1:282956/251‑1 (MQ=255)                       gACCACCAGTTCTAAAGAAACGCGTTCTTTGCCGAGCGCCTGCGCTTTCTCTGCATCGTTCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGAt                                                                                                                                                                                                               >  1:769515/1‑251 (MQ=255)                               gTTCTAAAGAAACGCGTTCTTTGCCGAGCGCCTGCGCTTTCTCTGCATCGTTCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAg                                                                                                                                                                                                                   >  1:541485/1‑239 (MQ=255)                               gTTCTAAAGAAACGCGTTCTTTGCCGAGCGCCTGCGCTTTCTCTGCATCGTTCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCggg                                                                                                                                                                                                       >  1:744019/1‑251 (MQ=255)                               gTTCTAAAGAAACGCGTTCTTTGCCGAGCGCCTGCGCTTTCTCTGCATCGTTCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCggg                                                                                                                                                                                                       >  1:861438/1‑251 (MQ=255)                               gTTCTAAAGAAACGCGTTCTTTGCCGAGCGCCTGCGCTTTCTCTGCATCGTTCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCggg                                                                                                                                                                                                       >  1:605583/1‑251 (MQ=255)                               gTTCTAAAGAAACGCGTTCTTTGCCGAGCGCCTGCGCTTTCTCTGCATCGTTCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCggg                                                                                                                                                                                                       >  1:293481/1‑251 (MQ=255)                                        aaaCGCGTTCTTTGCCGAGCGCCTGCGCTTTCTCTGCATCGTTCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGt                                                                                                                                                                                               <  1:602089/250‑1 (MQ=255)                                         aaCGCGTTCTTTGCCGAGCGCCTGCGCTTTCTCTGCATCGTTCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTag                                                                                                                                                                                             <  1:269416/251‑1 (MQ=255)                                           cgcgTTCTTTGCCGAGCGCCTGCGCTTTCTCTGCATCGTTCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTagag                                                                                                                                                                                           <  1:418094/251‑1 (MQ=255)                                                  tttGCCGAGCGCCTGCGCTTTCTCTGCATCGTTCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGcgaacga                                                                                                                                                                                    <  1:412753/251‑1 (MQ=255)                                                     gCCGAGCGCCTGCGCTTTCTCTGCATCGTTCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGc                                                                                                                                                                                          <  1:88963/242‑1 (MQ=255)                                                             ccTGCGCTTTCTCTGCATCGTTCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGc                                                                                                                                                                                                          >  1:426177/1‑218 (MQ=255)                                                                gcgcTTTCTCTGCATCGTTCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGa                                                                                                                                                                      >  1:457178/1‑251 (MQ=255)                                                                   cTTTCTCTGCATCGTTCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAAt                                                                                                                                                                   <  1:77070/251‑1 (MQ=255)                                                                      tctcTGCATCGTTCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATaa                                                                                                                                                                                                                    <  1:535011/199‑1 (MQ=255)                                                                         ctGCATCGTTCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCtgtg                                                                                                                                                             >  1:347396/1‑251 (MQ=255)                                                                                 ttCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGAt                                                                                                                                                                                                                         <  1:91995/183‑1 (MQ=255)                                                                                   ccATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGt                                                                                                                                                                                               <  1:109685/207‑1 (MQ=255)                                                                                   ccATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCAt                                                                                                                                                   >  1:109166/1‑251 (MQ=255)                                                                                       aCGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAAcg                                                                                                                                               >  1:644562/1‑251 (MQ=255)                                                                                        cGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGt                                                                                                                                                                                               >  1:208230/1‑202 (MQ=255)                                                                                             ggCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAAc                                                                                                                                                                           <  1:774686/217‑1 (MQ=255)                                                                                                   tCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACtt                                                                                                                                   <  1:572113/251‑1 (MQ=255)                                                                                                   tCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACtt                                                                                                                                   <  1:19636/251‑1 (MQ=255)                                                                                                    ccAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACttt                                                                                                                                  <  1:314293/251‑1 (MQ=255)                                                                                                         tCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATaa                                                                                                                             <  1:50985/251‑1 (MQ=255)                                                                                                                 gACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACg                                                                                                                       >  1:134491/1‑249 (MQ=255)                                                                                                                   cttcttCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGc                                                                                                                                                                                                                              <  1:262130/144‑1 (MQ=255)                                                                                                                      cttctAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGAt                                                                                                                                                                                                                         >  1:147219/1‑146 (MQ=255)                                                                                                                      cttctAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACtg                                                                                                                                                                         <  1:284216/194‑1 (MQ=255)                                                                                                                      cttctAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAAtt                                                                                                                >  1:154491/1‑251 (MQ=255)                                                                                                                      cttctAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAAtt                                                                                                                >  1:150287/1‑251 (MQ=255)                                                                                                                       ttctAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAAttt                                                                                                               <  1:718413/251‑1 (MQ=255)                                                                                                                          tAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCAc                                                                                                            <  1:692810/251‑1 (MQ=255)                                                                                                                          tAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCAc                                                                                                            <  1:899555/251‑1 (MQ=255)                                                                                                                          tAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCAc                                                                                                            <  1:244230/251‑1 (MQ=255)                                                                                                                           aaaCGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCACt                                                                                                           <  1:85955/251‑1 (MQ=255)                                                                                                                                cTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGc                                                                                                                      >  1:564302/1‑235 (MQ=255)                                                                                                                                cTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCACTTAttt                                                                                                      >  1:566866/1‑251 (MQ=255)                                                                                                                                           cGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCACTTATTTAGGAACAACAg                                                                                           <  1:813205/251‑1 (MQ=255)                                                                                                                                                    cAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCACTTATTTAGGAACAACAGAAATGaaaa                                                                                  >  1:463234/1‑251 (MQ=255)                                                                                                                                                                cGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCACTTATTTAGGAACAACAGAAATGAAAAAAATTATtacta                                                                      <  1:797317/251‑1 (MQ=255)                                                                                                                                                                         gAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTagag                                                                                                                                                                                           >  1:867295/1‑125 (MQ=255)                                                                                                                                                                         gAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCACTTATTTAGGAACAACAGAAATGAAAAAAATTATTACTACGCTTtct                                                              >  1:419117/1‑250 (MQ=255)                                                                                                                                                                         gAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCACTTATTTAGGAACAACAGAAATGAAAAAAATTATTACTACGCTTtct                                                              >  1:712111/1‑250 (MQ=255)                                                                                                                                                                          aaGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCACTTATTTAGGAACAACAGAAATGAAAAAAATTATTACTACGCTTTctct                                                            <  1:151890/251‑1 (MQ=255)                                                                                                                                                                          aaGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCACTTATTTAGGAACAACAGAAATGAAAAAAATTATTACTACGCTTTctct                                                            <  1:813542/251‑1 (MQ=255)                                                                                                                                                                                      ttAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCACTTATTTAGGAACAACAGAAATGAAAAAAATTATTACTACGCTTTCTCTTTTGGCTATATc                                                <  1:708199/251‑1 (MQ=255)                                                                                                                                                                                          aTCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCACTTATTTAGGAACAACAGAAATGAAAAAAATTATTACTACGCTTTCTCTTTTGGCTATATCTATg                                            <  1:23101/251‑1 (MQ=255)                                                                                                                                                                                            cGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCACTTATTTAGGAACAACAGAAATGAAAAAAATTATTACTACGCTTTCTCTTTTGGCTATATCTATGAg                                          <  1:589542/251‑1 (MQ=255)                                                                                                                                                                                            cGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCACTTATTTAGGAACAACAGAAATGAAAAAAATTATTACTACGCTTTCTCTTTTGGCTATATCTATGAg                                          <  1:381404/251‑1 (MQ=255)                                                                                                                                                                                               cGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCACTTATTTAGGAACAACAGAAATGAAAAAAATTATTACTACGCTTTCTCTTTTGGCTATATCTATGAgtg                                        <  1:109493/250‑1 (MQ=255)                                                                                                                                                                                                 attttattttTGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCACTTATTTAGGAACAACAGAAATGAAAAAAATTATTACTACGCTTTCTCTTTTGGCTATATCTATGAGTGTaa                                     >  1:595749/1‑251 (MQ=255)                                                                                                                                                                                                          ttGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCACTTATTTAGGAACAACAGAAATGAAAAAAATTATTACTACGCTTTCTCTTTTGGCTATATCTATGAGTGTAACGGCAAatg                            >  1:658573/1‑251 (MQ=255)                                                                                                                                                                                                          ttGATAGGATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCACTTATTTAGGAACAACAGAAATGAAAAAAATTATTACTACGCTTTCTCTTTTGGCTATATCTATGAGTGTAACGGCAAatg                            >  1:584787/1‑251 (MQ=255)                                                                                                                                                                                                                 gATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCACTTATTTAGGAACAACAGAAATGAAAAAAATTATTACTACGCTTTCTCTTTTGGCTATATCTATGAg                                          <  1:605246/230‑1 (MQ=255)                                                                                                                                                                                                                 gATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCACTTATTTAGGAACAACAGAAATGAAAAAAATTATTACTACGCTTTCTCTTTTGGCTATATCTATGAGTGTAACGGCAAATGATGTTg                      >  1:678199/1‑250 (MQ=255)                                                                                                                                                                                                                 gATTAATTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCACTTATTTAGGAACAACAGAAATGAAAAAAATTATTACTACGCTTTCTCTTTTGGCTATATCTATGAGTGTAACGGCAAATGATGTTg                      >  1:392198/1‑250 (MQ=255)                                                                                                                                                                                                                      aTTTCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAAc                                                                                                                                                                           <  1:559368/96‑1 (MQ=255)                                                                                                                                                                                                                       tttCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAAt                                                                                                                                                                   >  1:537799/1‑103 (MQ=255)                                                                                                                                                                                                                       tttCAAACATGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCACTTATTTAGGAACAACAGAAATGAAAAAAATTATtac                                                                        <  1:395558/194‑1 (MQ=255)                                                                                                                                                                                                                                tGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGa                                                                                                                                                            <  1:605713/101‑1 (MQ=255)                                                                                                                                                                                                                                tGATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCACTTATTTAGGAACAACAGAAATGAAAAAAATTATtac                                                                        >  1:346387/1‑185 (MQ=255)                                                                                                                                                                                                                                 gATAATGAATGTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCACTTATTTAGGAACAACAGAAATGaaaaaaa                                                                               >  1:512043/1‑177 (MQ=255)                                                                                                                                                                                                                                    aatgaatgTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCACTTATTTAGGAACAACAGAAATGAAAAAAATTATTACTACGCTTTCTCTTTTGGCTATATCTATGAGTGTAACGGCAAATGATGTTGCACTGAAAAGTAAATTGGaa  >  1:539447/1‑251 (MQ=255)                                                                                                                                                                                                                                    aatgaatgTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCACTTATTTAGGAACAACAGAAATGAAAAAAATTATTACTACGCTTTCTCTTTTGGCTATATCTATGAGTGTAACGGCAAATGATGTTGCACTGAAAAGTAAATTGGaa  >  1:652688/1‑251 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                    GTTCCAGATTTTTAATGGTGTTGACCACCAGTTCTAAAGAAACGCGTTCTTTGCCGAGCGCCTGCGCTTTCTCTGCATCGTTCCATACGTCAGGCTGTTCCAGTTCGGCGTTGACTTCTTCTAAACGCTCCAGTTTGGCGTCGAAGTCAAAGATACCCCCGAAGCACGGAAGTGCGGTCGGTTAAATCGGCGATTTTATTTTTGATAGGATTAATTTCAAACATGATAATGAATTTCGTAAAAAGGTAAATTAAGTGCGGGATTATAAGGGATTTTGCGGGAATTGGGTAGAGCGAACGAAACTCCAACTGTGAAATTCTGTGAATTTTGCATAACGCGTCAATTACTTTTATAATTCACGCTCAATTTCACTTATTTAGGAACAACAGAAATGAAAAAAATTATTACTACGCTTTCTCTTTTGGCTATATCTATGAGTGTAACGGCAAATGATGTTGCACTGAAAAGTAAATTGGAA  >  1/800003‑800480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑