Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	798,439	C→T	P211P (CCG→CCA)	unknown ←	Lysyl‑tRNA synthetase (class II) (EC 6.1.1.6)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	798,439	0	C	T	100.0%	188.8 / NA	70	P211P (CCG→CCA)	unknown	Lysyl‑tRNA synthetase (class II) (EC 6.1.1.6)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/30);  total (40/30)

GTTGTCCATTAAATCATGGTAATCGGCGTAGGCTTGGTAGTATTCCAGCATGGTGAATTCGGGATTGTGACGCACGGAAACACCTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCCGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGAAATTAAATCC‑AAATAACGTTGACGATAACGCACTTCTTGGTCGGTTAAACCGTGGAATTTGTCCGGCAGCGGGCGAAGGGCTTTGGTTAATAACTGAACTTTGGTGGCTTTCACGGTGAGT  >  1/798205‑798679                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                  gttgtCCATTAAATCATGGTAATCGGCGTAGGCTTGGTAGTATTCCAGCATGGTGAATTCGGGATTGTGACGCACGGAAACACCTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACa                                                                                                                                                                                                                                   >  1:874506/1‑251 (MQ=255)      ccATTAAATCATGGTAATCGGCGTAGGCTTGGTAGTATTCCAGCATGGTGAATTCGGGATTGTGACGCACGGAAACACCTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTggg                                                                                                                                                                                                                              >  1:719413/1‑251 (MQ=255)       cATTAAATCATGGTAATCGGCGTAGGCTTGGTAGTATTCCAGCATGGTGAATTCGGGATTGTGACGCACGGAAACACCTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGAAAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTgggg                                                                                                                                                                                                                             <  1:190646/251‑1 (MQ=255)       cATTAAATCATGGTAATCGGCGAAGGCTTGGTAGTATTCCAGCATGGTGAATTCGGGATTGGGACGCACGGAAACACCTTCATTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAATCGTTTTAAGTAAAGTTCCGGTGGGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGGGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTgggg                                                                                                                                                                                                                             <  1:894824/251‑1 (MQ=255)              tCATGGTAATCGGCGTAGGCTTGGTAGTATTCCAGCATGGTGAATTCGGGATTGTGACGCACGGAAACCCCTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCAc                                                                                                                                                                                                                      <  1:655588/251‑1 (MQ=255)               cATGGTAATCGGCGTAGGCTTGGTAGTATTCCAGCATGGTGAATTCGGGATTGTGACGCACGGAAACACCTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACt                                                                                                                                                                                                                     <  1:662653/251‑1 (MQ=255)                aTGGTAATCGGCGTAGGCTTGGTAGTATTCCAGCATGGTGAATTCGGGATTGTGACGCACGGAAACACCTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACtt                                                                                                                                                                                                                    >  1:521834/1‑251 (MQ=255)                 tGGTAATCGGCGTAGGCTTGGTAGTATTCCAGCATGGTGAATTCGGGATTGTGACGCCCGGAAACACCTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTc                                                                                                                                                                                                                   <  1:855160/251‑1 (MQ=255)                          gCGTAGGCTTGGTAGTATTCCAGCATGGTGAATTCGGGATTGTGACGCACGGAAACACCTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGa                                                                                                                                                                                                              >  1:475293/1‑247 (MQ=255)                          gCGTAGGCTTGGTAGTATTCCAGCATGGTGAATTCGGGATTGTGACGCACGGAAACACCTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGc                                                                                                                                                                                                           >  1:759251/1‑250 (MQ=255)                          gCGTAGGCTTGGTAGTATTCCAGCATGGTGAATTCGGGATTGTGACGCACGGAAACACCTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGc                                                                                                                                                                                                           >  1:454416/1‑250 (MQ=255)                          gCGTAGGCTTGGTAGTATTCCAGCATGGTGAATTCGGGATTGTGACGCACGGAAACACCTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGc                                                                                                                                                                                                           >  1:531377/1‑250 (MQ=255)                          gCGTAGGCTTGGTAGTATTCCAGCATGGTGAATTCGGGATTGTGACGCACGGAAACACCTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGc                                                                                                                                                                                                           >  1:893488/1‑250 (MQ=255)                                 cTTGGTAGTATTCCAGCATGGTGAATTCGGGATTGTGACGCACGGAAACACCTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTggaa                                                                                                                                                                                                   <  1:652710/251‑1 (MQ=255)                                   tGGTAGTATTCCAGCATGGTGAATTCGGGATTGTGACGCACGGAAACACCTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTggaaag                                                                                                                                                                                                 >  1:656022/1‑251 (MQ=255)                                     gtagtaTTCCAGCATGGTGAATTCGGGATTGTGACGCACGGAAACACCTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTgggg                                                                                                                                                                                                                             <  1:523341/221‑1 (MQ=255)                                        gtaTTCCAGCATGGTGAATTCGGGATTGTGACGCACGGAAACACCTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAAc                                                                                                                                                                                                                                    <  1:176215/211‑1 (MQ=255)                                         taTTCCAGCATGGTGAATTCGGGATTGTGACGCACGGAAACACCTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAata                                                                                                                                                                                           >  1:367261/1‑251 (MQ=255)                                            tCCAGCATGGTGAATTCGGGATTGTGACGCACGGAAACACCTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTc                                                                                                                                                                                        <  1:52684/251‑1 (MQ=255)                                             ccAGCATGGTGAATTCGGGATTGTGACGCACGGAAACACCTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCa                                                                                                                                                                                       >  1:80460/1‑251 (MQ=255)                                                         aTTCGGGATTGTGACGCACGGAAACACCTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGcc                                                                                                                                                                                                          >  1:646397/1‑220 (MQ=255)                                                                 ttGTGACGCACGGAAACACCTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACttt                                                                                                                                                                   >  1:896091/1‑251 (MQ=255)                                                                      aCGCAGGGAAACCCCTTCGTGGCGGAAATTGCGGTACAATTCGAATACGCTTGCAAAGCCGCCCACCGCTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTTAAAGGAATTGTGGTGTGTGACAAACGGTCGAGGCGCTGCACCACCTGGAATCGCTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGaa                                                                                                                                                               <  1:622137/250‑1 (MQ=255)                                                                      aCGCACGGAAACACCTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAAc                                                                                                                                                              <  1:100410/251‑1 (MQ=255)                                                                         cACGGAAACACCTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGaa                                                                                                                                                           >  1:796858/1‑251 (MQ=255)                                                                           cGGAAACCCCTTCGTTGAGGAAATTGCGGTTCAATTCAAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATAGCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAAt                                                                                                                                                                                                                                              <  1:742498/166‑1 (MQ=255)                                                                             gAAACACCTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATc                                                                                                                                                                                                                                             >  1:493305/1‑165 (MQ=255)                                                                               aaCACCTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAg                                                                                                                                                                                                            >  1:590086/1‑196 (MQ=255)                                                                                accccTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCCCTAAACGTTTTAAGTAAATTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGaa                                                                                                                                                    <  1:253544/248‑1 (MQ=255)                                                                                 ccccTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAg                                                                                                                                                                                                            <  1:663634/192‑1 (MQ=255)                                                                                   ccTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGt                                                                                                                                                 >  1:564949/1‑251 (MQ=255)                                                                                          tGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTGAAGTAAGGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGTTGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGcggcgg                                                                                                                                          <  1:522203/251‑1 (MQ=255)                                                                                            cGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCggg                                                                                                                                         <  1:523088/250‑1 (MQ=255)                                                                                                  ttGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGAttcctt                                                                                                                                  >  1:475768/1‑248 (MQ=255)                                                                                                          cAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGaaa                                                                                                                          >  1:834799/1‑251 (MQ=255)                                                                                                            aTTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAg                                                                                                                                                                 <  1:468372/210‑1 (MQ=255)                                                                                                                        gTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGAAATTAAATCC‑AAATaa                                                                                                           >  1:57731/1‑251 (MQ=255)                                                                                                                           cAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGAAATTAAATCC‑AAATAACGt                                                                                                        >  1:359728/1‑251 (MQ=255)                                                                                                                                     caccacTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTcc                                                                                                                                                                                                                        >  1:726009/1‑130 (MQ=255)                                                                                                                                                 ttttAAGTAAAGTTCCGGTGCGATCCGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCCGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGAAATTAAATCC‑AAATAACGTTGACGATAACGCACTTCTTGGt                                                                                  <  1:427021/251‑1 (MQ=255)                                                                                                                                                 ttttAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGAAATTAAATCC‑AAATAACGTTGACGATAACGCACTTCTTGGt                                                                                  <  1:893277/251‑1 (MQ=255)                                                                                                                                                          aaGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGAAATTAAATCC‑AAATAACGTTGACGATAACGCACTTCTTGGTCGGTTAAAc                                                                         <  1:381337/251‑1 (MQ=255)                                                                                                                                                          aaGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGAAATTAAATCC‑AAATAACGTTGACGATAACGCACTTCTTGGTCGGTTAAAc                                                                         <  1:135147/251‑1 (MQ=255)                                                                                                                                                            gTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGAAATTAAATCC‑AAATAACGTTGACGATAACGCACTTCTTGGTCGGTTAAACCg                                                                       >  1:516485/1‑251 (MQ=255)                                                                                                                                                                  gTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTggaa                                                                                                                                                                                                   <  1:97705/122‑1 (MQ=255)                                                                                                                                                                       aTACGCAAATCCATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGAAATTAAATCC‑AAATAACGTTGACGATAACGCACTTCTTGGTCGGTTAAACCGTGGAATTTGTc                                                            <  1:518164/251‑1 (MQ=255)                                                                                                                                                                         aCGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGCAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGAAATTAAATCC‑AAATAAAGTTTACGATAACGCACTTCTTGGTCGGTTTAACCGAGGGATTTTTCCg                                                          >  1:392500/1‑251 (MQ=255)                                                                                                                                                                          cGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGAAATTAAATCCAAAATAACGTTGACGATAAAGCACTTCTTGGt                                                                                  >  1:702175/1‑227 (MQ=255)                                                                                                                                                                          cGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGAAATTAAATCC‑AAATAACGTTGACGATAACGCACTTCTTGGTCGGTTAAACCGTGGAATTTGTCCgg                                                         >  1:698890/1‑251 (MQ=255)                                                                                                                                                                          cGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGAAATTAAATCC‑AAATAACGTTGACGATAACGCACTTCTTGGTCGGTTAAACCGTGGAATTTGTCCgg                                                         >  1:699574/1‑251 (MQ=255)                                                                                                                                                                          cGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGAAATTAAATCC‑AAATAACGTTGACGATAACGCACTTCTTGGTCGGTTAAACCGTGGAATTTGTCCgg                                                         >  1:471806/1‑251 (MQ=255)                                                                                                                                                                                tACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTgggg                                                                                                                                                                                                                             <  1:393376/82‑1 (MQ=255)                                                                                                                                                                                tACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGAAATTAAATCC‑AAATAACGTTGACGATAACGCACTTCTTGGTCGGTTAAACCGTGGAATTTGTCCGGCAGCgg                                                   >  1:264736/1‑251 (MQ=255)                                                                                                                                                                                tACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGAAATTAAATCC‑AAATAACGTTGACGATAAAGCACTTCTTGGTCGGTTAAGCCGTGGAATTTGTCCGGCAGCgg                                                   >  1:238521/1‑251 (MQ=255)                                                                                                                                                                                      tCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTACAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGAAATTAAATCC‑AAATAACGTTGACGATAACGCACTTCTTGGTCGGTTAAACCGTGGAATTTGTCCGGCAGCGGGCGAAg                                             >  1:867403/1‑251 (MQ=255)                                                                                                                                                                                          cGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGAAATTAAATCC‑AAATAACGTTGACGATAACGCACTTCTTGGt                                                                                  <  1:553267/210‑1 (MQ=255)                                                                                                                                                                                          cGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGAAATTAAATCC‑AAATAACGTTGACGATAACGCACTTCTTGGTCGGTTAAACCGTGGAATTTGTCCGGCAGCGGGCGAAggg                                           >  1:259407/1‑249 (MQ=255)                                                                                                                                                                                          cGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGAAATTAAATCC‑AAATAACGTTGACGATAACGCACTTCTTGGTCGGTTAAACCGTGGAATTTGTCCGGCAGCGGGCGAAggg                                           >  1:264445/1‑249 (MQ=255)                                                                                                                                                                                          cGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGAAATTAAATCC‑AAATAACGTTGACGATAACGCACTTCTTGGTCGGGTAAAc                                                                         >  1:657513/1‑219 (MQ=255)                                                                                                                                                                                           gTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTgcgg                                                                                                                                             <  1:414874/151‑1 (MQ=255)                                                                                                                                                                                           gTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGAAATTAAATCC‑AAATAACg                                                                                                         >  1:575249/1‑186 (MQ=255)                                                                                                                                                                                           gTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGAAATTAAATCC‑AAATAACGTTGACCATAACGCACTTCTTGGTCGGTTAAACCGTGGAATTTGTCCGGCAGCGGGCGAAGGGCtt                                        >  1:522462/1‑251 (MQ=255)                                                                                                                                                                                            tCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGAAATTAAATCC‑AAATAACGTTGACGAt                                                                                                 <  1:183411/193‑1 (MQ=255)                                                                                                                                                                                                  gCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAAc                                                                                                                                                              <  1:776759/127‑1 (MQ=255)                                                                                                                                                                                                    aTTGTGATGTGTGACACACGGACGAGCCGCTGCACCACCTGGAATCCCTTGCAACATTGGGGTTTCCACTTCCATGACGCCTTTGGCAAGGAAATCTTCACGAATGCCGGCGACGACTTTAGAACGAATGATGCAAGTGCGGCGGGATTCTTCGTTAGAAATTTAATCC‑AAATAACGTTGACGGTAACGCCCTTCTTTGTCCGTTAAACCGCGGAATTAGTCCGGCGGCGGGCTAAGGGCTTGGGTtaata                               >  1:879361/1‑251 (MQ=255)                                                                                                                                                                                                      tgtgATGTGTGACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGAAATTAAATCC‑AAATAACGTTGACGATAACGCACTTCTTGGTCGGTTAAACCGTGGAATTTGTCCGGCAGCGGGCGAAGGGCTTTGGTTAATAAc                             <  1:84765/251‑1 (MQ=255)                                                                                                                                                                                                              gtgACAAACGGACGAGCCGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTAGTTTGAAATTAAATCC‑AAATAACGTTGACGATAAAGCACTTCTTGGTCGGTTAAACCGTGGAATTTGTCCGGCAGCGGGCGAAGGGCTTTGGTTAATAACTGAAcctt                     >  1:95056/1‑248 (MQ=255)                                                                                                                                                                                                                              ccGCTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGAAATTAAATCC‑AAATAACGTTGACGATAACGCACTTCTTGGTCGGTTAAACCGTGGAATTTGTCCGGCAGCGGGCGAAGGGCTTTGGTTAATAACTGAACTTTGGTGGCTTTCACGGTg     <  1:101650/251‑1 (MQ=255)                                                                                                                                                                                                                                 cTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGAAATTAAATCC‑AAATAACGTTGACGATAACGCACTTCTTGGTCGGTTAAACCGTGGAATTTGTCCGGCAGCGGGCGAAGGGCTTTgg                                     >  1:488393/1‑216 (MQ=255)                                                                                                                                                                                                                                 cTGCACCACCTGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGAAATTAAATCC‑AAATAACGTTGACGATAACGCACTTCTTGGTCGGTTAAACCGTGGAATTTGTCCGGCAGCGGGCGAAGGGCTTTGGTTAATAACTGAACTTTGGTGGCTTTCACGGTGAGt  >  1:661571/1‑251 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                  GTTGTCCATTAAATCATGGTAATCGGCGTAGGCTTGGTAGTATTCCAGCATGGTGAATTCGGGATTGTGACGCACGGAAACACCTTCGTTGCGGAAATTGCGGTTCAATTCGAATACGCGTTCAAAGCCGCCCACCACTAAACGTTTTAAGTAAAGTTCCGGTGCGATACGCAAATACATATCCACGTCTAAGGCATTGTGATGTGTGACAAACGGACGAGCCGCTGCACCACCCGGAATCACTTGCAACATTGGGGTTTCCACTTCCATGAAGCCTTTGGAAAGGAAATATTCACGAATGCCGGCGACGACTTTAGAACGAATGATGAAAGTGCGGCGGGATTCTTCGTTAGAAATTAAATCC‑AAATAACGTTGACGATAACGCACTTCTTGGTCGGTTAAACCGTGGAATTTGTCCGGCAGCGGGCGAAGGGCTTTGGTTAATAACTGAACTTTGGTGGCTTTCACGGTGAGT  >  1/798205‑798679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑