Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	790,711	A→G	S294S (AGT→AGC)	unknown ←	Multidrug efflux system EmrAB‑OMF, membrane fusion component EmrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	790,711	0	A	G	100.0%	163.3 / NA	59	S294S (AGT→AGC)	unknown	Multidrug efflux system EmrAB‑OMF, membrane fusion component EmrA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/40);  total (19/40)

TTCCAGCGCACTTTGGTCGTAATCTAGGGTGTTGGTGGAGTAGAGCGGTGTCGTGCGTTTCTTCTGCGATAAGATTTCACCGCTCGTGTCGGCAATATTCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCACTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGTTGGGTTTCTTTGAAATTGGCATCCACCCACATTTTGTCGTTGGAAATAACAGCCATTAATGTACTGCCAACAGCGACTTTTTGCCCGATTTGTGTGCTGCGACGTGCCACATAACCGTCCACCGGGCTGACAA  >  1/790471‑790952                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  ttCCAGCGCACTTTGGTCGTAATCTAGGGTGTTGGTGGAGTAGAGCGGTGTCGTGCGTTTCTTCTGCGATAAGATTTCACCGCTCGTGTCGGCAATATTCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTg                                                                                                                                                                                                                                          >  1:24032/1‑250 (MQ=255)             ttGGTCGTAATCTAGGGTGTTGGTGGAGTAGAGCGGTGTCGTGCGTTTCTTCTGCGATAAGATTTCACCGCTCGTGTCGGCAATATTCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGc                                                                                                                                                                                                                             <  1:436857/251‑1 (MQ=255)                    tAATCTAGGGTGTTGGTGGAGTAGAGCGGTGTCGTGCGTTTCTTCTGCGATAAGATTTCACCGCTCGTGTCGGCAATATTCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTAc                                                                                                                                                                                                                      <  1:870630/251‑1 (MQ=255)                     aaTCTAGGGTGTTGGTGGAGTAGAGCGGTGTCGTGCGTTTCTTCTGCGATAAGATTTCACCGCTCGTGTCGGCAATATTCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTa                                                                                                                                                                                                                       >  1:459074/1‑249 (MQ=255)                      aTCTAGGGTGTTGGTGGAGTAGAGCGGTGTCGTGCGTTTCTTCTGCGATAAGATTTCACCGCTCGTGTCGGCAATATTCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTAc                                                                                                                                                                                                                      <  1:166136/249‑1 (MQ=255)                      aTCTAGGGTGTTGGTGGAGTAGAGCGGTGTCGTGCGTTTCTTCTGCGATAAGATTTCACCGCTCGTGTCGGCAATATTCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACtt                                                                                                                                                                                                                    <  1:599084/251‑1 (MQ=255)                            ggTGTTGGTGGAGTAGAGCGGTGTCGTGCGTTTCTTCTGCGATAAGATTTCACCGCTCGTGTCGGCAATATTCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCa                                                                                                                                                                                                               <  1:541185/250‑1 (MQ=255)                                ttGGTGGAGTAGAGCGGTGTCGTGCGTTTCTTCTGCGATAAGATTTCACCGCTCGTGTCGGCAATATTCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGaa                                                                                                                                                                                                          >  1:479859/1‑251 (MQ=255)                                ttGGTGGAGTAGAGCGGTGTCGTGCGTTTCTTCTGCGATAAGATTTCACCGCTCGTGTCGGCAATATTCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGaa                                                                                                                                                                                                          <  1:341612/251‑1 (MQ=255)                                ttGGTGGAGTAGAGCGGTGTCGTGCGTTTCTTCTGCGATAAGATTTCACCGCTCGTGTCGGCAATATTCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGaa                                                                                                                                                                                                          >  1:176572/1‑251 (MQ=255)                                     ggAGTAGAGCGGTGTCGTGCGTTTCTTCTGCGATAAGATTTCACCGCTCGTGTCGGCAATATTCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTca                                                                                                                                                                                                     >  1:675955/1‑251 (MQ=255)                                     ggAGTAGAGCGGTGTCGTGCGTTTCTTCTGCGATAAGATTTCACCGCTCGTGTCGGCAATATTCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTca                                                                                                                                                                                                     >  1:576107/1‑251 (MQ=255)                                      gAGTAGAGCGGTGTCGTGCGTTTCTTCTGCGATAAGATTTCACCGCTCGTGTCGGCAATATTCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTca                                                                                                                                                                                                     >  1:634119/1‑250 (MQ=255)                                                gtgtCGTGCGTTTCTTCTGCGATAAGATTTCACCGCTCGTGTCGGCAATATTCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTcac                                                                                                                                                                                                    <  1:765099/241‑1 (MQ=255)                                                gtgtCGTGCGTTTCTTCTGCGATAAGATTTCACCGCTCGTGTCGGCAATATTCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCa                                                                                                                                                                                          >  1:641914/1‑251 (MQ=255)                                                gtgtCGTGCGTTTCTTCTGCGATAAGATTTCACCGCTCGTGTCGGCAATATTCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCa                                                                                                                                                                                          >  1:316491/1‑251 (MQ=255)                                                   tCGTGCGTTTCTTCTGCGATAAGATTTCACCGCTCGTGTCGGCAATATTCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCatat                                                                                                                                                                                       <  1:791846/251‑1 (MQ=255)                                                        cGTTTCTTCTGCGATAAGATTTCACCGCTCGTGTCGGCAATATTCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAAt                                                                                                                                                                                   <  1:187021/250‑1 (MQ=255)                                                        cGTTTCTTCTGCGATAAGATTTCACCGCTCGTGTCGGCAATATTCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAAt                                                                                                                                                                                   <  1:782897/250‑1 (MQ=255)                                                                          aTTTCCCCGCTCGTGTCGGCAATATTCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCg                                                                                                                                                                 <  1:320822/250‑1 (MQ=255)                                                                          aTTTCACCGCTCGTGTCGGCAATATTCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCgg                                                                                                                                                                <  1:731212/251‑1 (MQ=255)                                                                          aTTTCACCGCTCGTGTCGGCAATATTCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCgg                                                                                                                                                                <  1:225570/251‑1 (MQ=255)                                                                                     cGTGTCGGCAATATTCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGAtt                                                                                                                                                      >  1:505244/1‑250 (MQ=255)                                                                                            gCAATATTCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCatat                                                                                                                                               >  1:162342/1‑250 (MQ=255)                                                                                               atatTCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCttt                                                                                                                                           >  1:652758/1‑251 (MQ=255)                                                                                                tatTCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTc                                                                                                                                          <  1:407849/251‑1 (MQ=255)                                                                                                   tCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGt                                                                                                                                       <  1:360323/251‑1 (MQ=255)                                                                                                   tCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGt                                                                                                                                       <  1:87522/251‑1 (MQ=255)                                                                                                              aaGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCAcat                                                                                                                                                                                                  >  1:263152/1‑181 (MQ=255)                                                                                                               aGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTAc                                                                                                                                                                                                                      >  1:299471/1‑160 (MQ=255)                                                                                                                ggTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGTTGGGTTTCTTTGa                                                                                                                          >  1:190798/1‑251 (MQ=255)                                                                                                                  tAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGTTGGGTTTCTTTGaaa                                                                                                                        <  1:616349/251‑1 (MQ=255)                                                                                                                          ccGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGTTGGGTTTCTTTGAAATTGGCa                                                                                                                  <  1:116395/249‑1 (MQ=255)                                                                                                                                      gggTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGTTGGGTTTCTTTGAAATTGGCATCCACCCACAtttt                                                                                                    >  1:80554/1‑251 (MQ=255)                                                                                                                                      gggTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGTTGGGTTTCTTTGAAATTGGCATCCACCCACAtt                                                                                                      <  1:590487/249‑1 (MQ=255)                                                                                                                                       ggTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGTTGGGTTTCTTTGAAATTGGCATCCACCCACATTTTg                                                                                                   >  1:508618/1‑251 (MQ=255)                                                                                                                                          aaCGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGTTGGGTTTCTTTGAAATTGGCATCCACCCACATTTTGTCg                                                                                                <  1:329679/251‑1 (MQ=255)                                                                                                                                                          cgcgTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGTTGGGTTTCTTTGAAATTGGCATCCACCCACATTTTGTCGTTGGAAATAACAGcc                                                                                 <  1:496053/250‑1 (MQ=255)                                                                                                                                                               cTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGTTGGGTTTCTTTGAAATTGGCATCCACCCACATTTTGTCGTTGGAAATAACAGCCATTAAt                                                                           <  1:260653/251‑1 (MQ=255)                                                                                                                                                               cTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGTTGGGTTTCTTTGAAATTGGCATCCACCCACATTTTGTCGTTGGAAATAACAGCCATTAAt                                                                           <  1:342702/251‑1 (MQ=255)                                                                                                                                                                tAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGTTGGGTTTCTTTGAAATTGGCATCCACCCACATTTTGTCGTTGGAAATAACAGCCATTAATg                                                                          <  1:826438/251‑1 (MQ=255)                                                                                                                                                                 aaGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGTTGGGTTTCTTTGAAATTGGCATCCACCCACATTTTGTCGTTGGAAATAACAGCCATTAATGt                                                                         <  1:538140/251‑1 (MQ=255)                                                                                                                                                                 aaGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGTTGGGTTTCTTTGAAATTGGCATCCACCCACATTTTGTCGTTGGAAATAACAGCCATTAATGt                                                                         >  1:619507/1‑251 (MQ=255)                                                                                                                                                                    gaaatgggcACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGTTGGGTTTCTTTGAAATTGGCATCCACCCACATTTTGTCGTTGGAAATAACAGCCATTAATGt                                                                         <  1:2043/241‑1 (MQ=255)                                                                                                                                                                    cTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGTTGGGTTTCTTTGAAATTGGCATCCACCCACATTTTGTCGTTGGAAATAACAGCCATTAATGTAc                                                                       <  1:5356/250‑1 (MQ=255)                                                                                                                                                                    cTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGTTGGGTTTCTTTGAAATTGGCATCCACCCACATTTTGTCGTTGGAAATAACAGCCATTAATGTAc                                                                       <  1:192877/250‑1 (MQ=255)                                                                                                                                                                      aaCGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGTTGGGTTTCTTTGAAATTGGCATCCACCCACATTTTGTCGTTGGAAATAACAGCCATTAATGTACTGc                                                                    <  1:759427/251‑1 (MQ=255)                                                                                                                                                                                    cccGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTAc                                                                                                                                                                   >  1:368559/1‑142 (MQ=255)                                                                                                                                                                                        ttGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGTTGGGTTTCTTTGAAATTGGCATCCACCCACATTTTGTCGTTGGAAATAACAGCCATTAATGTACTGCCAACAGCGACTTTTTGcc                                                  <  1:135379/251‑1 (MQ=255)                                                                                                                                                                                        ttGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGTTGGGTTTCTTTGAAATTGGCATCCACCCACATTTTGTCGTTGGAAATAACAGCCATTAATGTACTGCCAACAGCGACTTTTTGcc                                                  <  1:364265/251‑1 (MQ=255)                                                                                                                                                                                                cTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCGTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGTCAGTTTTACCGGTTGTCCGATTc                                                                                                                                                     <  1:347051/144‑1 (MQ=255)                                                                                                                                                                                                  ttGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGTTGGGTTTCTTTGAAATTGGCATCCACCCACATTTTGTCGTTGGAAATAACAGCCATTAATGTACTGCCAACAGCGACTTTTTGCCCGATTtgtgt                                        <  1:851109/251‑1 (MQ=255)                                                                                                                                                                                                           ttGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGTTGGGTTTCTTTGAAATTGGCATCCACCCACATTTTGTCGTTGGAAATAACAGCCATTAATGTACTGCCAACAGCGACTTTTTGCCCGATTTGTGTGCTGCGACg                               <  1:26767/251‑1 (MQ=255)                                                                                                                                                                                                              ccGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGTTGGGTTTCTTTGAAATTGGCATCCACCCACATTTTGTCGTTGGAAATAACAGCCATTAATGTACTGCCAACAGCGACTTTTTGCCCGATTTGTGTGCTGCGACGTGc                            <  1:549869/251‑1 (MQ=255)                                                                                                                                                                                                                     cATTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGTTGGGTTTCTTTGAAATTGGCATCCACCCACATTTTGTCGTTGGAAATAACAGCCATTAATGTACTGcc                                                                   <  1:233924/205‑1 (MQ=255)                                                                                                                                                                                                                      aTTTTGTGCAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGTTGGGTTTCTTTGAAATTGGCATCCACCCACATTTTGTCGTTGGAAATAACAGCCATTAATGTACTGCCAACAGCGACTTTTTGCCCGATTTGTGTGCTGCGACGTGCCACATAAc                    <  1:31397/251‑1 (MQ=255)                                                                                                                                                                                                                              cAGGCAATAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGTTGGGTTTCTTTGAAATTGGCATCCACCCACATTTTGTCGTTGGAAATAACAGCCATTAATGTACTGCCAACAGCGACTTTTTGCCCGATTTGTGTGCTGCGACGTGCCACATAACCGTCCAc             <  1:610753/250‑1 (MQ=255)                                                                                                                                                                                                                                    aTAAAGAAAATGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGTTGGGTTTCTTTGAAATTGGCATCCACCCACATTTTGTCGTTGGAAATAACAGCCATTAAt                                                                           <  1:97960/182‑1 (MQ=255)                                                                                                                                                                                                                                          aaaaTGCGCTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGTTGGGTTTCTTTGAAATTGGCATCCACCCACATTTTGTCGTTGGAAATAACAGCCATTAATGTACTGCCAACAGCGACTTTTTGCCCGATTTGTGTGCTGCGACGTGCCACATAACCGTCCACCGGGCTGACaa  <  1:225132/249‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  TTCCAGCGCACTTTGGTCGTAATCTAGGGTGTTGGTGGAGTAGAGCGGTGTCGTGCGTTTCTTCTGCGATAAGATTTCACCGCTCGTGTCGGCAATATTCACTTCAACCAAGGTAGATAAGCCGATTCGCAAGGGGTAACGGGCAAGTTGTTGCGCGTCTAAGCTAACGCGCACCGGCACCCGTTGTATCACTTTGATCCAGTTGCCGGTGGCATTTTGTGCAGGCAATAAAGAAAATGCACTGCCGGTGCCCATTTCAATGCCTTCTACTTTGCCATCGAATTTCACATCATGACCATATAAATCAAAAGACAGTTTTACCGGTTGTCCGATTCGCATATCTTTCAGTTGGGTTTCTTTGAAATTGGCATCCACCCACATTTTGTCGTTGGAAATAACAGCCATTAATGTACTGCCAACAGCGACTTTTTGCCCGATTTGTGTGCTGCGACGTGCCACATAACCGTCCACCGGGCTGACAA  >  1/790471‑790952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑