Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	786,250	A→G	T738A (ACA→GCA)	unknown →	Valyl‑tRNA synthetase (EC 6.1.1.9)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	786,250	0	A	G	100.0%	206.9 / NA	75	T738A (ACA→GCA)	unknown	Valyl‑tRNA synthetase (EC 6.1.1.9)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/35);  total (40/35)

GCTTCGTGCTTACCAATGAAAAGCTGGATTTAAGCCAAGGCGAAATTGAATTTTCCGTTGCCGATCGTTGGATCCAATCGGAATTCAACCGCACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGACAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGTTGCAGCCGTTCCCGCAAGTGGACATGACGGCGTTTGATGTGGACGCGGAAGCCGAAATCGCATGGTTGAAAGAGGTCATCGTCGCCGTACGTAATATTCGCGCCGAATGCAACAT  >  1/786005‑786494                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     gCTTCGTGCTTACCAATGAAAAGCTGGATTTAAGCCAAGGCGAAATTGAATTTTCCGTTGCCGATCGTTGGATCCAATCGGAATTCAACCGCACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAg                                                                                                                                                                                                                                                  >  1:503341/1‑250 (MQ=255)    tCGTGCTTACCAATGAAAAGCTGGATTTAAGCCAAGGCGAAATTGAATTTTCCGTTGCCGATCGTTGGATCCAATCGGAATTCAACCGCACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAg                                                                                                                                                                                                                                              <  1:120090/251‑1 (MQ=255)      gTGCTTACCAATGAAAAGCTGGATTTAAGCCAAGGCGAAATTGAATTTTCCGTTGCCGATCGTTGGATCCAATCGGAATTCAACCGCACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGa                                                                                                                                                                                                                                             >  1:827842/1‑250 (MQ=255)       tGCTTACCAATGAAAAGCTGGATTTAAGCCAAGGCGAAATTGAATTTTCCGTTGCCGATCGTTGGATCCAATCGGAATTCAACCGCACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGAc                                                                                                                                                                                                                                            <  1:325997/250‑1 (MQ=255)           tACCAATGAAAAGCTGGATTTAAGCCAAGGCGAAATTGAATTTTCCGTTGCCGATCGTTGGATCCAATCGGAATTCAACCGCACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTgg                                                                                                                                                                                                                                       >  1:715367/1‑251 (MQ=255)             ccAATGAAAAGCTGGATTTAAGCCAAGGCGAAATTGAATTTTCCGTTGCCGATCGTTGGATCCAATCGGAATTCAACCGCACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTg                                                                                                                                                                                                                                     <  1:379139/251‑1 (MQ=255)              cAATGAAAAGCTGGATTTAAGCCAAGGCGAAATTGAATTTTCCGTTGCCGATCGTTGGATCCAATCGGAATTCAACCGCACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGc                                                                                                                                                                                                                                    <  1:586220/251‑1 (MQ=255)                     aaGCTGGATTTAAGCCAAGGCGAAATTGAATTTTCCGTTGCCGATCGTTGGATCCAATCGGAATTCAACCGCACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGtt                                                                                                                                                                                                                             >  1:211571/1‑251 (MQ=255)                         tGGATTTAAGCCAAGGCGAAATTGAATTTTCCGTTGCCGATCGTTGGATCCAATCGGAATTCAACCGCACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTagag                                                                                                                                                                                                                         <  1:832241/251‑1 (MQ=255)                           gATTTAAGCCAAGGCGAAATTGAATTTTCCGTTGCCGATCGTTGGATCCAATCGGAATTCAACCGCACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGAcc                                                                                                                                                                                                                                           >  1:662297/1‑231 (MQ=255)                              ttAAGCCAAGGCGAAATTGAATTTTCCGTTGCCGATCGTTGGATCCAATCGGAATTCAACCGCACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAAtt                                                                                                                                                                                                                    <  1:715623/251‑1 (MQ=255)                                 aGCCAAGGCGAAATTGAATTTTCCGTTGCCGATCGTTGGATCCAATCGGAATTCAACCGCACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTa                                                                                                                                                                                                                   >  1:846118/1‑249 (MQ=255)                                   ccAAGGCGAAATTGAATTTTCCGTTGCCGATCGTTGGATCCAATCGGAATTCAACCGCACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTg                                                                                                                                                                                                                <  1:871757/250‑1 (MQ=255)                                      aGGCGAAATTGAATTTTCCGTTGCCGATCGTTGGATCCAATCGGAATTCAACCGCACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGtt                                                                                                                                                                                                            >  1:321088/1‑251 (MQ=255)                                        gCGAAATTGAATTTTCCGTTGCCGATCGTTGGATCCAATCGGAATTCAACCGCACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTCGGTGCATGTGTTAGAGAAATTACTGCGTTTa                                                                                                                                                                                                          >  1:647106/1‑251 (MQ=255)                                            aaTTGAATTTTCCGTTGCCGATCGTTGGATCCAATCGGAATTCAACCGCACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAgcgc                                                                                                                                                                                                      <  1:455365/251‑1 (MQ=255)                                                gAATTTTCCGTTGCCGATCGTTGGATCCAATCGGAATTCAACCGCACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCAcc                                                                                                                                                                                                   >  1:827889/1‑250 (MQ=255)                                                     ttCCGTTGCCGATCGTTGGATCCAATCGGAATTCAACCGCACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTa                                                                                                                                                                                             >  1:550275/1‑251 (MQ=255)                                                        cGTTGCCGATCGTTGGATCCAATCGGAATTCAACCGCACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTc                                                                                                                                                                                          <  1:490429/251‑1 (MQ=255)                                                        cGTTGCCGATCGTTGGATCCAATCGGAATTCAACCGCACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTc                                                                                                                                                                                          <  1:729485/251‑1 (MQ=255)                                                        cGTTGCCGATCGTTGGATCCAATCGGAATTCAACCGCACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTc                                                                                                                                                                                          <  1:394837/251‑1 (MQ=255)                                                            gCCGATCGTTGGATCCAATCGGAATTCAACCGCACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCcatt                                                                                                                                                                                      >  1:330803/1‑251 (MQ=255)                                                                 tCGTTGGATCCAATCGGAATTCAACCGCACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACt                                                                                                                                                                                                                 <  1:88812/219‑1 (MQ=255)                                                                  cGTTGGATCCAATCGGAATTCAACCGCACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTa                                                                                                                                                                                 >  1:316380/1‑250 (MQ=255)                                                                            aaTCGGAATTCAACCGCACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTTACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATc                                                                                                                                                                      <  1:582002/251‑1 (MQ=255)                                                                            aaTCGGAATTCAACCGCACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCAGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAAt                                                                                                                                                                       >  1:432908/1‑250 (MQ=255)                                                                                           gCACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTAGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGaaa                                                                                                                                                       >  1:433681/1‑251 (MQ=255)                                                                                            cACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAg                                                                                                                                                      >  1:323421/1‑251 (MQ=255)                                                                                              cTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGt                                                                                                                                                    >  1:631998/1‑251 (MQ=255)                                                                                                gtgGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGAc                                                                                                                                                                                                                                            >  1:364650/1‑161 (MQ=255)                                                                                                gtgGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGtt                                                                                                                                                                                              <  1:77157/207‑1 (MQ=255)                                                                                                gtgGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGttt                                                                                                                                                  >  1:477255/1‑251 (MQ=255)                                                                                                gtgGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGttt                                                                                                                                                  >  1:43912/1‑251 (MQ=255)                                                                                                                  gCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCAtgtg                                                                                                                                                                                                                               <  1:318506/156‑1 (MQ=255)                                                                                                                  gCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGa                                                                                                                                >  1:698617/1‑251 (MQ=255)                                                                                                                   cATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCAt                                                                                                                                         <  1:262526/241‑1 (MQ=255)                                                                                                                   cATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGa                                                                                                                                >  1:276336/1‑250 (MQ=255)                                                                                                                   cATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTACGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGa                                                                                                                                >  1:445963/1‑250 (MQ=255)                                                                                                                     ttAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAg                                                                                                                             <  1:399780/251‑1 (MQ=255)                                                                                                                        aGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAgg                                                                                                                                                     <  1:171526/224‑1 (MQ=255)                                                                                                                        aGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCCGGCAGAAAGTGAAAgg                                                                                                                                                     <  1:488106/224‑1 (MQ=255)                                                                                                                             aTTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGt                                                                                                                     <  1:519839/251‑1 (MQ=255)                                                                                                                             aTTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGt                                                                                                                     <  1:179169/251‑1 (MQ=255)                                                                                                                                  gTTTTGACTTATGTGCCCCCGCGCTTTATGAATTCACTTGTAACCAATTCTGTGACTGGTATTTGGACCTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGc                                                                                                                                                                                                                                    <  1:675738/135‑1 (MQ=255)                                                                                                                                        aCTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGTTGCAGCCGtt                                                                                                           >  1:671304/1‑250 (MQ=255)                                                                                                                                         cTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGttt                                                                                                                                                  <  1:716073/210‑1 (MQ=255)                                                                                                                                            aTGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAgg                                                                                                                                                     <  1:483869/204‑1 (MQ=255)                                                                                                                                            aTGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGTTGCAg                                                                                                                >  1:514795/1‑241 (MQ=255)                                                                                                                                                    ccGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGTTGCAGCCGTTCCCGCAAGTGGAc                                                                                              <  1:193391/251‑1 (MQ=255)                                                                                                                                                         cTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGTTGCAGCCGTTCCCGCAAGTGGACATGa                                                                                          >  1:504231/1‑250 (MQ=255)                                                                                                                                                               gAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGTTGCAGCCGTTCCCGCAAGTGGACATGACGGCGtt                                                                                   >  1:858917/1‑251 (MQ=255)                                                                                                                                                                    cACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGTTGCAGCCGTTCCCGCAAGTGGACATGACGGCGTTTGAtg                                                                              >  1:532930/1‑251 (MQ=255)                                                                                                                                                                     aCTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGTTGCAg                                                                                                                >  1:230420/1‑216 (MQ=255)                                                                                                                                                                      cTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAg                                                                                                                                                                <  1:326717/167‑1 (MQ=255)                                                                                                                                                                          gAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGTTGCAGCCGTTCCCGCAAGTGGACATGACGGCGTTTGATGTGGAcg                                                                        >  1:740462/1‑251 (MQ=255)                                                                                                                                                                          gAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGTTGCAGCCGTTCCCGCAAGTGGACATGACGGCGTTTGATGTGGAc                                                                         <  1:377339/250‑1 (MQ=255)                                                                                                                                                                           aaCCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGTTGCAGCCGTTCCCGCAAGTGGACATg                                                                                           >  1:466477/1‑231 (MQ=255)                                                                                                                                                                                aTTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGTTGCAGCCGTTCCCGCAAGTGGACATGACGGCGTTTGATGTGGACGCGGAAg                                                                  >  1:756024/1‑251 (MQ=255)                                                                                                                                                                                aTTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGTTGCAGCCGTTCCCGCAAGTGGACATGACGGCGTTTGATGTGGACGCGGAAg                                                                  >  1:430751/1‑251 (MQ=255)                                                                                                                                                                                        aCTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGTTGCAGCCGTTCCCGCAAGTGGACATGACGGCGTTTGATGTGGACGCGGAAGCCGAAATc                                                          <  1:586949/251‑1 (MQ=255)                                                                                                                                                                                           ggTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGTTGCAGCCGTTCCCGCAAGTGGACATGACGGCGTTTGATGTGGACGCGGAAGCCGTAATCGCa                                                       >  1:24389/1‑251 (MQ=255)                                                                                                                                                                                            gTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGTTGCAGCCGTTCCCGCAAg                                                                                                   <  1:652606/206‑1 (MQ=255)                                                                                                                                                                                              aTTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGTTGCAGCCGTTCCCGCAAGTGGACATGACGGCGTTTGATGTGGACGCGGAAGCCGAAATCGCATgg                                                    >  1:276673/1‑251 (MQ=255)                                                                                                                                                                                              aTTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGTTGCAGCCGTTCCCGCAAGTGGACATGACGGCGTTTGATGTGGACGCGGAAGCCGAAATCGCATgg                                                    >  1:361602/1‑251 (MQ=255)                                                                                                                                                                                                      cTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGTTGCAGCCGTTCCCGCAAGTGGACATGACGGCGTTTGATGTGGACGCGGAAGCCGAAATCGCATGGTTGAAaga                                            <  1:542207/251‑1 (MQ=255)                                                                                                                                                                                                       tCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGTTGCAGCCGTTCCCGCAAGTGGACATGACGGCGTTTGATGTGGACGCGGAAGCCGAAATCGCATGGTTGAAagac                                           <  1:141700/251‑2 (MQ=255)                                                                                                                                                                                                       ccACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGTTGCAGCCGTTCCCGCAAGTGGACATGACGGCGTTTGATGTGGACGCGGAAGCCGAAATCGCATGGTTGAAagag                                           <  1:116118/250‑1 (MQ=255)                                                                                                                                                                                                        cACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCAtg                                                                                                                                                                                                                                 >  1:851203/1‑68 (MQ=255)                                                                                                                                                                                                        cACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGTTGCAGCCGTTCCCGCAAGTGGACATGACGGCGTTTGAtg                                                                              >  1:271529/1‑215 (MQ=255)                                                                                                                                                                                                        cACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGTTGCAGCCGTTCCCGCAAGTGGACATGACGGCGTTTGATGTGGACGCGGAAGCCGAAATCGCATGGTTGAAAGAgg                                          >  1:580367/1‑251 (MQ=255)                                                                                                                                                                                                                            aCGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGTTGCAGCCGTTCCCGCAAGTGGACATGACGGCGTTTGATGTGGACGCGGAAGCCGAAATCGCATGGTTGAAAGAGGTCATCGTCGCCGTACGTAat                      <  1:750722/251‑1 (MQ=255)                                                                                                                                                                                                                            aCGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGTTGCAGCCGTTCCCGCAAGTGGACATGACGGCGTTTGATGTGGACGCGGAAGCCGAAATCGCATGGTTGAAAGAGGTCATCGTCGCCGTACGTAat                      <  1:216305/251‑1 (MQ=255)                                                                                                                                                                                                                             cGGCAACCAAGCGCAAATTCGCGCGGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGTTGCAGCCGTTCCCGCAAGTGGACATGACGGCGTTTGATGTGGACGCGGAAGCCGAAATCGCATGGTTGAAAGAGGTCATCGTCGCCGTACGTAata                     >  1:87643/1‑251 (MQ=255)                                                                                                                                                                                                                                       gcgcAAATTCGCGCGGCAAGCCAGACCCTGGTGCATGTGTTAGAGAAATTACTGCGCTTAGCGCTCCCGGTTATTCCATTCATTACCGAAGAAATCTGGCCGAAAGTGAAAGGTTACGTCGGCATTGACGCCGACAGCATTATGTTGCAGTAGTTCCCGCAAGTGGACATGACGGCGTTTGATGTGGACGCGGAAGCCGAAATCGCATGGCTGAAag                                             >  1:73730/1‑217 (MQ=255)                                                                                                                                                                                                                                                cgcgcgGCAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGTTGCAGCCGTTCCCGCAAGTGGACATGACGGCGTTTGATGTGGACGCGGAAGCCGAAATCGCATGGTTGAAAGAGGTCATCGTCGCCGTACGTAATATTCGCGCCGAATGCAACAt  <  1:257372/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     GCTTCGTGCTTACCAATGAAAAGCTGGATTTAAGCCAAGGCGAAATTGAATTTTCCGTTGCCGATCGTTGGATCCAATCGGAATTCAACCGCACTGTGGAAACCTTCCGCAACGCATTAAGCCAATTCCGTTTTGACTTATGTGCCACCGCGCTTTATGAATTCACTTGGAACCAATTCTGTGACTGGTATTTGGAACTCACCAAGCCGGTATTCGCAAACGGCAACCAAGCGCAAATTCGCGCGACAAGCCAGACCTTGGTGCATGTGTTAGAGAAATTACTGCGTTTAGCGCACCCGGTTATTCCATTCATTACCGAAGAAATCTGGCAGAAAGTGAAAGGTTTCGTCGGCATTGACGCCGACAGCATTATGTTGCAGCCGTTCCCGCAAGTGGACATGACGGCGTTTGATGTGGACGCGGAAGCCGAAATCGCATGGTTGAAAGAGGTCATCGTCGCCGTACGTAATATTCGCGCCGAATGCAACAT  >  1/786005‑786494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑