Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	777,338	A→C	E29D (GAA→GAC)	unknown →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	777,338	0	A	C	100.0%	180.7 / NA	64	E29D (GAA→GAC)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (31/33);  total (31/33)

TCATGGTTCCTGCCACTAACAGCGTACTTCCTACGGTTTTGTTCATAGATTTTCCTTATTGTGTATTTAAAAGTTGACGGAGATTGTATTTTAAATTTTACATTCTGTAAAACAAAGATCGTGATATAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGAAACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAGCCAAATCAGCCATTGCACCTCTGAATTTGATGTAACGAATTATTTACAGTTAGACGAGAATATCCTCGATATTATCGTGCTGAAATGGTGTGACGGCAGTTATTTGGAGGAACAGGAT  >  1/777093‑777582                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     tCATGGTTCCTGCCACTAACAGCGTACTTCCTACGGTTTTGTTCATAGATTTTCCTTATTGTGTATTTAAAAGTTGACGGAGATTGTATTTTAAATTTTACATTCTGTAAAACAAAGATCGTGATATAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACAtt                                                                                                                                                                                                                                                 >  1:316482/1‑251 (MQ=255)       ttCCTGCCACTAACAGCGTACTTCCTACGGTTTTGTTCATAGATTTTCCTTATTGTGTATTTAAAAGTTGACGGAGATTGTATTTTAAATTTTACATTCTGTAAAACAAAGATCGTGATATAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTAcaca                                                                                                                                                                                                                                            <  1:66136/250‑1 (MQ=255)       ttCCTGCCACTAACAGCGTACTTCCTACGGTTTTGTTCATAGATTTTCCTTATTGTGTATTTAAAAGTTGACGGAGATTGTATTTTAAATTTTACATTCTGTAAAACAAAGATCGTGATATAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTAcaca                                                                                                                                                                                                                                            <  1:663297/250‑1 (MQ=255)           ttccACTAACAGGGTACTTCCTACGGTTTTGTTAATATATTTTCCTTATTGTGTATTTAAAAGTTGCCGGAGATTGTATTTTAAATTTTACATTCTGTAAAACAAAGATCGTGATATAAGCCCCAAAATCTTAATTTTAGGTTAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGTTTCCGCCATGACATTACTCAATTCCTTTCAGGATTTAGACACATTACAAATCAac                                                                                                                                                                                                                                       <  1:304189/249‑1 (MQ=255)            gCCACTAACAGCGTACTTCCTACGGTTTTGTTCATAGATTTTCCTTATTGTGTATTTAAAAGTTGACGGAGATTGTATTTTAAATTTTACATTCTGTAAAACAAAGATCGTGATATAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAaca                                                                                                                                                                                                                                      >  1:847463/1‑251 (MQ=255)              cACTAACAGCGTACTTCCTACGGTTTTGTTCATAGATTTTCCTTATTGTGTATTTAAAAGTTGACGGAGATTGTATTTTAAATTTTACATTCTGTAAAACAAAGATCGTGATATAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAacac                                                                                                                                                                                                                                     >  1:270519/1‑250 (MQ=255)                 tAACAGCGTACTTCCTACGGTTTTGTTCATAGATTTTCCTTATTGTGTATTTAAAAGTTGACGGAGATTGTATTTTAAATTTTACATTCTGTAAAACAAAGATCGTGATATAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAac                                                                                                                                                                                                                                       >  1:150907/1‑245 (MQ=255)                       cGTACTTCCTACGGTTTTGTTCATAGATTTTCCTTATTGTGTATTTAAAAGTTGACGGAGATTGTATTTTAAATTTTACATTCTGTAAAACAAAGATCGTGATATAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAacac                                                                                                                                                                                                                             <  1:625446/249‑1 (MQ=255)                              ccTACGGTTTTGTTCATAGATTTTCCTTATTGTGTATTTAAAAGTTGACGGAGATTGTATTTTAAATTTTACATTCTGTAAAACAAAGATCGTGATATAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTAtttt                                                                                                                                                                                                                    >  1:97013/1‑251 (MQ=255)                                                     tCCTTATTGTGTATTTAAAAGTTGACGGAGATTGTATTTTAAATTTTACATTCTGTAAAACAAAGATCGTGATATAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACAt                                                                                                                                                                                                                                           >  1:388532/1‑205 (MQ=255)                                                     tCCTTATTGTGTATTTAAAAGTTGACGGAGATTGTATTTTAAATTTTACATTCTGTAAAACAAAGATCGTGATATAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAg                                                                                                                                                                                             >  1:554422/1‑251 (MQ=255)                                                       cTTATTGTGTATTTAAAAGTTGACGGAGATTGTATTTTAAATTTTACATTCTGTAAAACAAAGATCGTGATATAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTg                                                                                                                                                                                           >  1:900802/1‑251 (MQ=255)                                                        ttATTGTGTATTTAAAAGTTGACGGAGATTGTATTTTAAATTTTACATTCTGTAAAACAAAGATCGTGATATAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAac                                                                                                                                                                                                                                       >  1:331925/1‑206 (MQ=255)                                                                      aaaGTTGACGGAGATTGTATTTTAAATTTTACATTCTGTAAAACAAAGATCGTGATATAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCaa                                                                                                                                                                            >  1:894625/1‑251 (MQ=255)                                                                                  gaTTGTATTTTAAATTTTACATTCTGTAAAACAAAGATCGTGATATAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTc                                                                                                                                                                                                                <  1:65055/203‑1 (MQ=255)                                                                                               aTTTTACATTCTGTAAAACAAAGATCGTGATATAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAagag                                                                                                                                                                         >  1:820828/1‑229 (MQ=255)                                                                                               aTTTTACATTCTGTAAAACAAAGATCGTGATATAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATgg                                                                                                                                                    <  1:463805/250‑1 (MQ=255)                                                                                                      aTTCTGTAAAACAAAGATCGTGATATAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAAc                                                                                                                                            <  1:531517/251‑1 (MQ=255)                                                                                                         cTGTAAAACAAAGATCGTGATATAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTAcaca                                                                                                                                                                                                                                            <  1:889449/152‑1 (MQ=255)                                                                                                         cTGTAAAACAAAGATCGTGATATAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAac                                                                                                                                                                                                                                       <  1:181322/157‑1 (MQ=255)                                                                                                         cTGTAAAACAAAGATCGTGATATAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGa                                                                                                                                                               <  1:503062/229‑1 (MQ=255)                                                                                                         cTGTAAAACAAAGATCGTGATATAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAAc                                                                                                                                            >  1:110351/1‑248 (MQ=255)                                                                                                           gTAAAACAAAGATCGTGATATAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTc                                                                                                                                                                                                                <  1:886375/178‑1 (MQ=255)                                                                                                           gTAAAACAAAGATCGTGATATAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCa                                                                                                                                       >  1:666288/1‑251 (MQ=255)                                                                                                               aaCAAAGATCGTGATATAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTc                                                                                                                                                                                                                >  1:147267/1‑174 (MQ=255)                                                                                                                       tCGTGATATAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATgg                                                                                                                                                    <  1:570691/226‑1 (MQ=255)                                                                                                                           gATATAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAg                                                                                                                        >  1:49941/1‑250 (MQ=255)                                                                                                                           gATATAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGCATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATgg                                                                                                                                                    <  1:455781/222‑1 (MQ=255)                                                                                                                            atatAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAGc                                                                                                                       <  1:361497/250‑1 (MQ=255)                                                                                                                            atatAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAGc                                                                                                                       <  1:670180/250‑1 (MQ=255)                                                                                                                               taAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAGCCaaa                                                                                                                   <  1:703198/251‑1 (MQ=255)                                                                                                                                          aTCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAac                                                                                                                                                                                                                                       <  1:31871/124‑1 (MQ=255)                                                                                                                                          aTCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAGCCAAATCAGCCATTg                                                                                                         >  1:118841/1‑250 (MQ=255)                                                                                                                                            cTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAGCCAAATCAGCCATTGCAc                                                                                                      <  1:641947/251‑1 (MQ=255)                                                                                                                                                           aaTTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAGCCAAATCAGCCATTGCACCTCTGAATTTGATGt                                                                                       >  1:408724/1‑251 (MQ=255)                                                                                                                                                           aaTTTATGGGGCGGATTGAGTACAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAGCCAAATCAGCCATTGCACCTCTGAATTTGATGt                                                                                       >  1:673602/1‑251 (MQ=255)                                                                                                                                                                         aTTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGc                                                                                                                                                                                                                                  >  1:607051/1‑98 (MQ=255)                                                                                                                                                                         aTTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATgg                                                                                                                                                    <  1:568265/176‑1 (MQ=255)                                                                                                                                                                         aTTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAGCCAAATCAGCCATTGCACCTCTGAATTTGATGTAACGAATTATTTa                                                                          >  1:362108/1‑250 (MQ=255)                                                                                                                                                                            gAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTc                                                                                                                                                                                                                >  1:43344/1‑113 (MQ=255)                                                                                                                                                                             aGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAGCCAAATCAGCCATTGCACCTCTGAATTTGATGTAACGAATTATTTACAGt                                                                      <  1:197464/250‑1 (MQ=255)                                                                                                                                                                                                ttCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAGCCAAATCAGCCATTGCACCTCTGAATTTGGTGTAACGAATTATTTACAGTTAGACGAGAATATCCTCGat                                                  <  1:217564/251‑1 (MQ=255)                                                                                                                                                                                                ttCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAGCCAAATCAGCCATTGCACCTCTGAATTTGATGTAACGAATTATTTACAGTTAGACGAGAATATCCTCGat                                                  <  1:170095/251‑1 (MQ=255)                                                                                                                                                                                                  ccGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAGCCAAATCAGCCATTGCACCTCTGAATTTGATGTAACGAATTATTTACAGTTAGACGAGAATATCCTCGatat                                                <  1:788106/251‑1 (MQ=255)                                                                                                                                                                                                       gTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAGCCAAATCAGCCATTGCACCTCTGAATTTGATGTAACGAATTATTTACAGTTAGACGAGAATATCCTCGATATTATCg                                           >  1:447674/1‑251 (MQ=255)                                                                                                                                                                                                        tAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAGCCAAATCAGCCATTGCACCTCTGAATTTGATGTAACGAATTATTTACAGTTAGACGAGAATATCCTCGATATTATCg                                           <  1:217009/250‑1 (MQ=255)                                                                                                                                                                                                               ttCCGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAGCCAAATCAGCCATTGCACCTCTGAATTTGATGTAACGAATTATTTACAGTTAGACGAGAATATCCTCGATATTATCGTGCTGaaa                                   <  1:207383/251‑1 (MQ=255)                                                                                                                                                                                                                 ccGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAGCCAAATCAGCCATTGCACCTCTGAATTTGATGTAACGAATTATTTACAGTTAGACGAGAATATCCTCGATATTATCGTGCTGAAATg                                 >  1:335385/1‑251 (MQ=255)                                                                                                                                                                                                                  cGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATGGCCAAATCAGCCATTGCACCTCTGAATTTGATGTAACGAATTATTTACAGTTAGACGAGAATATCCTCGATATTATCGTGCTGAAATgg                                <  1:391456/251‑1 (MQ=255)                                                                                                                                                                                                                  cGCCATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAGCCAAATCAGCCATTGCACCTCTGAATTTGATGTAACGAATTATTTACAGTTAGACGAGAATATcc                                                       >  1:653276/1‑228 (MQ=255)                                                                                                                                                                                                                    ccATGACATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAGCCAAATCAGCCATTGCACCTCTGAATTTGATGTAACGAATTATTTACAGTTAGACGAGAATATCCTCGATATTATCGTGCTGAAATGgtg                              <  1:525135/251‑1 (MQ=255)                                                                                                                                                                                                                         aCATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAac                                                                                                                                                                                                                               >  1:218292/1‑53 (MQ=255)                                                                                                                                                                                                                         aCATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAGCCAAATCAGCCATTGCACCTCTGAATTTGATGTAACGAATTATTTAc                                                                         <  1:756316/203‑1 (MQ=255)                                                                                                                                                                                                                         aCATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAGCCAAATCAGCCATTGCACCTCTGAATTTGATGTAACGAATTATTTACAGTTAGACGAGAATATCCTCGATATTATCGTGCTGAAATGGTGTGACg                         >  1:282327/1‑251 (MQ=255)                                                                                                                                                                                                                         aCATTACTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAGCCAAATCAGCCATTGCACCTCTGAATTTGATGTAACGAATTATTTACAGTTAGACGAGAATATCCTCGATATTATCGTGCTGAAATGGTGTGACg                         >  1:107017/1‑251 (MQ=255)                                                                                                                                                                                                                               cTCAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAAc                                                                                                                                            <  1:71337/130‑1 (MQ=255)                                                                                                                                                                                                                                 cAATTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAAc                                                                                                                                            >  1:769735/1‑128 (MQ=255)                                                                                                                                                                                                                                   aTTACTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAGCCAAATCAGCCATTGCACCTCTGAATTTGATGTAACGAATTATTTACAGTTAGACGAGAATATCCTCGAtattat                                             <  1:607265/221‑1 (MQ=255)                                                                                                                                                                                                                                      aCTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAGCCAAATCAGCCATTGCACCTCTGAATTTGATGTAACGAATTATTTACAGTTAGACGAGAATATCCTCGATATTATCGTGCTGAAATGGTGTGACGGCAGTTATTTgga            >  1:793711/1‑251 (MQ=255)                                                                                                                                                                                                                                       cTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGAc                                                                                                                                                                                                  >  1:345183/1‑68 (MQ=255)                                                                                                                                                                                                                                       cTTTCAGGATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAGCCAAATCAGCCATTGCACCTCTGAATTTGATGTAACGAATTATTTACAGTTAGACGAGAATATCCTCGAtattat                                             <  1:65424/217‑1 (MQ=255)                                                                                                                                                                                                                                              gATTTAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAGCCAAATCAGCCATTGCACCTCTGAATTTGATGTAACGAATTATTTACAGTTAGACGAGAATATCCTCGATATTATCGTGCTGAAATGGTGTGACGGCAGTTATTTgg             <  1:109971/242‑1 (MQ=255)                                                                                                                                                                                                                                                tttAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAGCCAAATCAGCCATTGCACCTCTGAATTTGATGTAACGAATTATTTACAGTTAGACGAGAATATCCTCGATATTATCGTGCTGAAATGgtg                              <  1:779517/223‑1 (MQ=255)                                                                                                                                                                                                                                                tttAGACACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAGCCAAATCAGCCATTGCACCTCTGAATTTGATGTAACGAATTATTTACAGTTAGACGAGAATATCCTCGATATTATCGTGCTGAAATGGTGTGACGGCAGTTATTTGGAGGAACAGGAt  >  1:276663/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     TCATGGTTCCTGCCACTAACAGCGTACTTCCTACGGTTTTGTTCATAGATTTTCCTTATTGTGTATTTAAAAGTTGACGGAGATTGTATTTTAAATTTTACATTCTGTAAAACAAAGATCGTGATATAAGCCGCAAAATCTTAATTTTAGGTGAAATTTATGGGGCGGATTGAGTATAGTAAAAATTTTCATTCCGCTGTAAGGGGTTCCGCCATGACATTACTCAATTACTTTCAGGATTTAGAAACATTACACATCAACACCGCAACACGCCTATTTTATTCCCCATGCCAATGACCAAAGTGCGGTGGAAAATCCAAGAGAATTTTACGATTATTTTATGGTGTTGAACGGGCAATTCGTGGGTTATAGCCAAATCAGCCATTGCACCTCTGAATTTGATGTAACGAATTATTTACAGTTAGACGAGAATATCCTCGATATTATCGTGCTGAAATGGTGTGACGGCAGTTATTTGGAGGAACAGGAT  >  1/777093‑777582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑