Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 772,155 C→A intergenic (‑184/‑164) unknown ← / → unknown PTS system, ascorbate‑specific IIC component / PTS system, ascorbate‑specific IIB component (EC 2.7.1.194)/L‑ascorbate‑6‑phosphate lactonase UlaG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1772,1550CA100.0% 46.4 / NA 19intergenic (‑184/‑164)unknown/unknownPTS system, ascorbate‑specific IIC component / PTS system, ascorbate‑specific IIB component (EC 2.7.1.194)/L‑ascorbate‑6‑phosphate lactonase UlaG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (8/11);  total (8/11)

CAGTAAGGGCGCTTTAGATAGGATCTGTTCGTTGATCCACATGAAGATTTCCATGGTAATGCTCCTTGAAATGTAGAGTTAATATAAATATTCATGCGTAAATTTCGTTTGTTGGCGGCGGATAATAAATATTTATAATGATTTTTCAAAGATGTTTTTTGATAAAATGTGAGCAATGTCACGATTTATTTTATTGAAATACTTATGTGTGATTAGAAATTTCGAATCAAGAGTTGGCGTTGTTATCTAAGGTTAAAATGTTAGCTAAGTCTGGAATTTAATAAATTTTTAACATATTTTAATAGAAAAATTT‑AAAAAAAAATATTCCATTAATGAAAATGATTGTTTATGATTATAAATGATTAAATTATTCTACTAAACCTAAAAAAAGGAGTTATTGTATGGCTAAAGTTAATGAAATCACCCGTGAAAGTTGGATCCTTTCTACTTTTCCTGAATGGGGCACTTGGTTAAAT  >  1/771918‑772393
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                               
cAGTAAGGGCGCTTTAGATAGGATCTGTTCGTTGATCCACATGAAGATTTCCATGGTAATGCTCCTTGAAATGTAGAGTTAATATAAATATTCATGCGTAAATTTCGTTTGTTGGCGGCGGATAATAAATATTTATAATGATTTTTCAAAGATGTTTTTTGATAAAATGTGAGCAATGTCACGATTTATTTTATTGAAATACTTATGTGTGATTAGAAATTTCGAATCAAGAGTTGGAGTTGTTATCTaa                                                                                                                                                                                                                                     <  1:712279/250‑1 (MQ=255)
                     gATCTGTTCGTTGATCCACATGAAGATTTCCATGGTAATGCTCCTTGAAATGTAGAGTTAATATAAATATTCATGCGTAAATTTCGTTTGTTGGCGGCGGATAATAAATATTTATAATGATTTTTCAAAGATGTTTTTTGATAAAATGTGAGCAATGTCACGATTTATTTTATTGAAATACTTATGTGTGATTAGAAATTTCGAATCAAGAGTTGGAGTTGTTATCTAAGGTTAAAATGTTAGCTAAGTc                                                                                                                                                                                                                <  1:406087/250‑1 (MQ=255)
                       tCTGTTCGTTGATCCACATGAAGATTTCCATGGTAATGCTCCTTGAAATGTAGAGTTAATATAAATATTCATGCGTAAATTGCGTTTGTTGGCGGCGGATAATAAATATTTATAATGATTTTTCAAAGATGTTTTTTGATAAAATGTGAGCAATGTCACGATTTATTTTATTGAAATACTTATGTGTGATTAGAAATTTCGAATCAAGAGTTGGAGTTGTTATCTAAGGTTAAAATGTTAGCTAAGTCTgg                                                                                                                                                                                                             <  1:447939/251‑1 (MQ=255)
                           ttCGTTGATCCACATGAAGATTTCCATGGTAATGCTCCTTGAAATGTAGAGTTAATATAAATATTCATGCGTAAATTTCGTTTGTTGGCGGCGGATAATAAATATTTATAATGATTTTTCAAAGATGTTTTTTGATAAAATGTGAGCAATGTCACGATTTATTTTATTGAAATACTTATGTGTGATTAGAAATTTCGAATCAAGAGTTGGAGTTGTTATCTAAGGTTAAAATGTTAGCTAAGTCTGGAAt                                                                                                                                                                                                          <  1:326299/250‑1 (MQ=255)
                           ttCGTTGATCCACATGAAGATTTCCATGGTAATGCTCCTTGAAATGTAGAGTTAATATAAATATTCATGCGTAAATTTCGTTTGTTGGCGGCGGATAATAAATATTTATAATGATTTTTCAAAGATGTTTTTTGATAAAATGTGAGCAATGTCACGATTTATTTTATTGAAATACTTATGTGTGATTAGAAATTTCGAATCAAGAGTTGGAGTTGTTATCTAAGGTTAAAATGTTAGCTAAGTCTGGAAt                                                                                                                                                                                                          <  1:413690/250‑1 (MQ=255)
                                  aTCCACATGAAGATTTCCATGGTAATGCTCCTTGAAATGTAGAGTTAATATAAATATTCATGCGTAAATTTCGTTTGTTGGCGGCGGATAATAAATATTTATAATGATTTTTCAAAGATGTTTTTTGATAAAATGTGAGCAATGTCACGATTTATTTTATTGAAATACTTATGTGTGATTAGAAATTTCGAATCAAGAGTTGGAGTTGTTATCTAAGGTTaaaa                                                                                                                                                                                                                             <  1:292780/224‑1 (MQ=255)
                                               tttCCATGGTAATGCTCCTTGAAATGTAGAGTTAATATAAATATTCATGCGTAAATTTCGTTTGTTGGCGGCGGATAATAAATATTTATAATGATTTTTCAAAGATGTTTTTTGATAAAATGTGAGCAATGTCACGATTTATTTTATTGAAATACTTATGTGTGATTAGAAATTTCGAATCAAGAGTTGGAGTTGTTATCTAAGGTTAAAATGTTAGCTAAGTCTGGAATTTAATAAATTTTTAACATAtt                                                                                                                                                                                     >  1:852044/1‑251 (MQ=255)
                                                       gTAATGCTCCTTGAAATGTAGAGTTAATATAAATATTCATGCGTAAATTTCGTTTGTTGGCGGCGGATAATAAATATTTATAATGATTTTTCAAAGATGTTTTTTGATAAAATGTGAGCAATGTCACGATTTATTTTATTGAAATACTTATGTGTGATTAGAAATTTCGAATCAAGAGTTGGAGTTGTTATCTAAGGTTAAAATg                                                                                                                                                                                                                           <  1:67425/205‑1 (MQ=255)
                                                                   gAAATGTAGAGTTAATATAAATATTCATGCGTAAATTTCGTTTGTTGGCGGCGGATAATAAATATTTATAATGATTTTTCAAAGATGTTTTTTGATAAAATGTGAGCAATGTCACGATTTATTTTATTGAAATACTTATGTGTGATTAGAAATTTCGAATCAAGAGTTGGAGTTGTTATCTAAGGTTAAAAt                                                                                                                                                                                                                            >  1:809006/1‑192 (MQ=255)
                                                                        gTAGAGTTAATATAAATATTCATGCGTAAATTTCGTTTGTTGGCGGCGGATAATAAATATTTATAATGATTTTTCAAAGATGTTTTTTGATAAAATGTGAGCAATGTCACGATTTATTTTATTGAAATACTTATGTGTGATTAGAAATTTCGAATCAAGAGTTGGAGTTGTTATCTAAGGTTAAAATGTTAGCTAAGTCTGGAATTTAATAAATTTTTAACATATTTTAATAGAAAAATTT‑aaaaaaaaag                                                                                                                                                           >  1:181147/1‑250 (MQ=255)
                                                                           gagTTAATATAAATATTCATGCGTAAATTTCGTTTGTTGGCGGCGGATAATAAATATTTATAATGATTTTTCAAAGATGTTTTTTGATAAAATGTGAGCAATGTCACGATTTATTTTATTGAAATACTTATGTGTGATTAGAAATTTCGAATCAAGAGTTGGAGTTGTTATCTAAGGTTAAAATGTTAGCTAAGTCTGGAATTTAATAAATTTTTAACATATTTTAATAGAAAAATTT‑AAAAAAAAAGAtt                                                                                                                                                        >  1:297921/1‑251 (MQ=255)
                                                                               tAATATAAATATTCATGCGTAAATTTCGTTTGTTGGCGGCGGATAATAAATATTTATAATGATTTTTCAAAGATGTTTTTTGATAAAATGTGAGCAATGTCACGATTTATTTTATTGAAATACTTATGTGTGATTAGAAATTTCGAATCAAGAGTTGGAGTTGTTATCTAAGGTTAAAATg                                                                                                                                                                                                                           >  1:121415/1‑181 (MQ=255)
                                                                                       atatTCATGCGTAAATTTCGTTTGTTGGCGGCGGATAATAAATATTTATAATGATTTTTCAAAGATGTTTTTTGATAAAATGTGAGCAATGTCACGATTTATTTTATTGAAATACTTATGTGTGATTAGAAATTTCGAATCAAGAGTTGGAGTTGTTATCTAAGGTTAAAATGTTAGCTAAGTCTGGAATTTAATAAATTTTTAACATATTTTAATAGAAAAATTT‑AAAAAAAAAGATTCCATTAATGaaa                                                                                                                                            >  1:795975/1‑251 (MQ=255)
                                                                                                                                                       aTGTTTTTTGATAAAATGTGAGCAATGTCACGATTTATTTTATTGAAATACTTATGTGTGATTAGAAATTTCGAATCAAGAGTTGGAGTTGTTATCTAAGGTTAAAATGTTAGCTAAGTCTGGAATTTAATAAATTTTTAACATATTTTAATAGAAAAATTT‑AAAAAAAAAGATTCCATTAATGAAAATGATTGTTTATGATTATAAATGATTAAATTATTCTACTAAACCTAAAAAAAGGAGTTATTg                                                                              <  1:413019/249‑1 (MQ=255)
                                                                                                                                                                                                 ttGAAATACTTATGTGTGATTAGAAATTTCGAATCAAGAGTTGGAGTTGTTATCTAAGGTTAAAATGTTAGCTAAGTCTGGAATTTAATAAATTTTTAACATATTTTAATAGAAAAATTTAAAAAAAAAAGATTCCATTAATGAAAATGATTGTTTATGATTATAAATGATTAAATTATTCTACTAAACCTAAAAAAAGGAGTTATTGTa                                                                            >  1:480584/1‑210 (MQ=255)
                                                                                                                                                                                                    aaaTACTTATGTGTGATTAGAAATTTCGAATCAAGAGTTGGAGTTGTTATCTAAGGTTAAAATGTTAGCt                                                                                                                                                                                                                     <  1:801390/70‑1 (MQ=255)
                                                                                                                                                                                                     aaTACTTATGTGTGATTAGAAATTTCGAATCAAGAGTTGGAGTTGTTATCTAAGGTTAAAATGTTAGCTAAGTCTGGAATTTAATAAATTTTTAACATATTTTAATAGAAAAATTT‑AAAAAAAAAGATTCCATTAATGAAAATGATTGTTTATGATTATAAATGATTAAATTATTCTACTAAACCTAAAAAAAGGAGTTATTGTATGGCTAAAGTTAATGAAATCACCCGTGAAAGTTGGATCCTTTCTAc                              <  1:557669/251‑1 (MQ=255)
                                                                                                                                                                                                      aTACTTATGTGTGATTAGAAATTTCGAATCAAGAGTTGGAGTTGTTATCTAAGGTTAAAATGTTAGCTAAGTc                                                                                                                                                                                                                >  1:388145/1‑73 (MQ=255)
                                                                                                                                                                                                                                 aTCAAGAGTTGGAGTTGTTATCTAAGGTTAAAATGTTAGCTAAGTCTGGAATTTAATAAATTTTTAACATATTTTAATAGAAAAATTT‑AAAAAAAAAGATTCCATTAATGAAAATGATTGTTTATGATTATAAATGATTAAATTATTCTACTAAACCTAAAAAAAGGAGTTATTGTATGGCTAAAGTTAATGAAATCACCCGTGAAAGTTGGATCCTTTCTACTTTTCCTGAATGGGGCACTTGGTTAAAt  <  1:39274/251‑1 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                               
CAGTAAGGGCGCTTTAGATAGGATCTGTTCGTTGATCCACATGAAGATTTCCATGGTAATGCTCCTTGAAATGTAGAGTTAATATAAATATTCATGCGTAAATTTCGTTTGTTGGCGGCGGATAATAAATATTTATAATGATTTTTCAAAGATGTTTTTTGATAAAATGTGAGCAATGTCACGATTTATTTTATTGAAATACTTATGTGTGATTAGAAATTTCGAATCAAGAGTTGGCGTTGTTATCTAAGGTTAAAATGTTAGCTAAGTCTGGAATTTAATAAATTTTTAACATATTTTAATAGAAAAATTT‑AAAAAAAAATATTCCATTAATGAAAATGATTGTTTATGATTATAAATGATTAAATTATTCTACTAAACCTAAAAAAAGGAGTTATTGTATGGCTAAAGTTAATGAAATCACCCGTGAAAGTTGGATCCTTTCTACTTTTCCTGAATGGGGCACTTGGTTAAAT  >  1/771918‑772393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑