Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	757,702	C→T	R11R (CGC→CGT)	unknown →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	757,702	0	C	T	100.0%	93.5 / NA	36	R11R (CGC→CGT)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/21);  total (15/21)

CGGGGTGAATAAAAACAACATAGCAGATTATGCCAAGTTAGGGATAAATTTATTTATCATCTCCGCGCCTTACTATGCACCGCCGGAAGATATTAAAGTGGTGATTGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCAC‑TTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGCACTTTTTATTCCCCTCCACAATTCAACAAAATAGAATTTATACCCTTAAATAAATTGCGGATAAACCGCTCTTTTTCTTTGGAAAATATCGGATTTATTGCCATTTTCAGGTAACTTTCCTTTTCGGTTTTGGCGGAAAATACGTTATAATCCCGACCAATTTTTTATCGATAAAATGACCGAGATTTCTATGTTAAAAAAAGTGACCGAAAGTTTATTGGTGCAGAGC  >  1/757466‑757931                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                      cggGGTGAATAAAAACAACATAGCAGATTATGCCAAGTTAGGGATAAATTTATTTATCATCTCCGCGCCTTACTATGCACCGCCGGAAGATATTAAAGTGGTGATTGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCACTTTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTcc                                                                                                                                                                                                                           <  1:174574/250‑1 (MQ=255) cggGGTGAATAAAAACAACATAGCAGATTATGCCAAGTTAGGGATAAATTTATTTATCATCTCCGCGCCTTACTATGCACCGCCGGAAGATATTAAAGTGGTGATTGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCACTTTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTcc                                                                                                                                                                                                                           <  1:501845/250‑1 (MQ=255)          tAAAAACAACATAGCAGATTATGCCAAGTTAGGGATAAATTTATTTATCATCTCCGCGCCTTACTATGCACCGCCGGAAGATATTAAAGTGGTGATTGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCACTTTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTCCCCTCCACAAt                                                                                                                                                                                                                 <  1:844050/251‑1 (MQ=255)               acaacaTAGCAGATTATGCCAAGTTAGGGATAAATTTATTTATCATCTCCGCGCCTTACTATGCACCGCCGGAAGATATTAAAGTGGTGATTGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCACTTTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTCCCCTCCACAATTcaac                                                                                                                                                                                                            <  1:828359/251‑1 (MQ=255)                  acaTAGCAGATTATGCCAAGTTAGGGATAAATTTATTTATCATCTCCGCGCCTTACTATGCACCGCCGGAAGATATTAAAGTGGTGATTGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCACTTTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTc                                                                                                                                                                                                                            <  1:174160/232‑1 (MQ=255)                       gCAGATTATGCCAAGTTAGGGATAAATTTATTTATCATCTCCGCGCCTTACTATGCACCGCCGGAAGATATTAAAGTGGTGATTGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCACTTTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTcccc                                                                                                                                                                                                                         <  1:422546/230‑1 (MQ=255)                       gCAGATTATGCCAAGTTAGGGATAAATTTATTTATCATCTCCGCGCCTTACTATGCACCGCCGGAAGATATTAAAGTGGTGATTGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCACTTTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTCCCCTCCACAATTCAACAAAATAGa                                                                                                                                                                                                    >  1:278401/1‑251 (MQ=255)                           aTTATGCCAAGTTAGGGATAAATTTATTTATCATCTCCGCGCCTTACTATGCACCGCCGGAAGATATTAAAGTGGTGATTGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCACTTTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTCCCCTCCACAATTCAACAAAATAGAAttt                                                                                                                                                                                                <  1:214345/251‑1 (MQ=255)                                 ccAAGTTAGGGATAAATTTATTTATCATCTCCGCGCCTTACAATGCACCGCCGGAAGATATTAAAGTGGTGATTGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCACTTTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTCCCCTCCACAATTCAACAAAATAGAATTTATAcc                                                                                                                                                                                           <  1:644628/250‑1 (MQ=255)                                 ccAAGTTAGGGATAAATTTATTTATCATCTCCCGGCCTAACAAGGCCCCGCCGAAGGATATTAAATTGTTGA‑TGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCACTTTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTCCCCTCCACAATTCAACAAAATAGAATTTATAccc                                                                                                                                                                                          <  1:699015/250‑1 (MQ=255)                                       tAGGGATAAATTTATTTATCATCTCCGCGCCTTACTATGCACCGCCGGAAGATATTAAAGTGGTGATTGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCACTTTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTCCCCTCCACAATTCAACAAAATAGAATTTATACCCTtaaat                                                                                                                                                                                    <  1:57777/251‑1 (MQ=255)                                                       tatCATCTCCGCGCCTTACTATGCACCGCCGGAAGATATTAAAGTGGTGATTGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCACTTTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTCCCCTCCACaa                                                                                                                                                                                                                  <  1:885191/205‑1 (MQ=255)                                                                 gcgcCTTACTATGCACCGCCGGAAGATATTAAAGTGGTGATTGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCACTTTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTCCCCTCCACAATTCAACAAAATAGAATTTATAc                                                                                                                                                                                            <  1:488041/217‑1 (MQ=255)                                                                 gcgcCTTACTATGCACCGCCGGAAGATATTAAAGTGGTGATTGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCACTTTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTCCCCTCCACAATTCAACAAAATAGAATTTATACCCTTAAATAAATTGCGGATAAACCGCTCttttt                                                                                                                                                           >  1:29537/1‑250 (MQ=255)                                                                  cgcCTTACTATGCACCGCCGGAAGATATTAAAGTGGTGATTGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCACTTTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTCCCCTCCACAATTCAACAAAATAGAATTTATACCCTTAAATAAATTGCGGATAAACCGCTCTTTTTc                                                                                                                                                          <  1:723930/250‑1 (MQ=255)                                                                    ccTTACTATGCACCGCCGGAAGATATTAAAGTGGTGATTGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCACTTTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTc                                                                                                                                                                                                                            >  1:510752/1‑182 (MQ=255)                                                                    ccTTACTATGCACCGCCGGAAGATATTAAAGTGGTGATTGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCACTTTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTCCCCTCcac                                                                                                                                                                                                                    <  1:203827/190‑1 (MQ=255)                                                                    ccTTACTATGCACCGCCGGAAGATATTAAAGTGGTGATTGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCACTTTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTCCCCTCCACAATTCAACAAAATAGAATTTATACCCTTAAATAAATTGCGGATAAACCGCTCTTTTTCttt                                                                                                                                                       >  1:169977/1‑251 (MQ=255)                                                                      ttACTATGCACCGCCGGAAGATATTAAAGTGGTGATTGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCACTTTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTCCCCTCCACAATTcaacaa                                                                                                                                                                                                          >  1:9838/1‑198 (MQ=255)                                                                       tACTATGCACCGCCGGAAGATATTAAAGTGGTGATTGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCACTTTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACtt                                                                                                                                                                                                                                   <  1:277136/172‑1 (MQ=255)                                                                             gCACCGCCGGAAGATATTAAAGTGGTGATTGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCACTTTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTCCCCTCCACAATTCAACAAAATAGAATTTATACCCTTAAATAAATTGCGGATAAACCGCTCTTTTTCTTTGGAAAatat                                                                                                                                              >  1:678244/1‑251 (MQ=255)                                                                                ccgccgGAAGATATTAAAGTGGTGATTGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCACTTTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTCCCCTCCACAATTCAACAAAATAGAATTTATACCCTTAAATAAATTGCGGATAAAc                                                                                                                                                                     >  1:342164/1‑225 (MQ=255)                                                                                   ccgGAAGATATTAAAGTGGTGATTGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCACTTTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTCCCCTCCACAATTCAACAAAATAGAATTTATACCCTTAAATAAATTGCGGATAAACCGCTCTTTTTCTTTGGAAAATATCGGAtt                                                                                                                                        >  1:825050/1‑251 (MQ=255)                                                                                          atatTAAAGTGGTGATTGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCACTTTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTa                                                                                                                                                                                                                               <  1:800536/157‑1 (MQ=255)                                                                                          atatTAAAGTGGTGATTGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCACTTTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTcc                                                                                                                                                                                                                           >  1:392120/1‑161 (MQ=255)                                                                                          atatTAAAGTGGTGATTGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCACTTTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTCCCCTc                                                                                                                                                                                                                       >  1:750574/1‑165 (MQ=255)                                                                                          atatTAAAGTGGTGATTGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCACTTTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTCCCCTCCACAATTCAACAAAATAGAATTTATAccc                                                                                                                                                                                          >  1:479488/1‑194 (MQ=255)                                                                                          atatTAAAGTGGTGATTGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCACTTTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTCCCCTCCACAATTCAACAAAATAGAATTTATACCCTtaaat                                                                                                                                                                                    <  1:702181/200‑1 (MQ=255)                                                                                                                                             aCCGCACTTTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTCCCCTCCACAATTCAACAAAATAGAATTTATACCCTTAAATAAATTGCGGATAAACCGCTCTTTTTCTTTGGAAAATATCGGATTTATTGCCATTTTCAGGTAACTTTCCTTTTCGGTTTTGGCGGAAAATACGTTATAATcc                                                                              <  1:552089/251‑1 (MQ=255)                                                                                                                                                       tttttAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTCCCCTCCACAATTCAACAAAATAGAATTTATACCCTTAAATAAATTGCGGATAAACCGCTCTTTTTCTTTGGAAAATATCGGATTTATTGCCATTTTCAGGt                                                                                                                       >  1:771693/1‑200 (MQ=255)                                                                                                                                                                                gCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTCCCCTCCACAATTCAACAAAATAGAATTTATACCCTTAAATAAATTGCGGATAAACCGCTCTTTTTCTTTGGAAAATATCGGATTTATTGCCATTTTCAGGTAACTTTCCTTTTCGGTTTTGGCGGAAAATACGTTATAATCCCGACCAATTTTTTATCGATAAAATGACCGAGAttt                                           >  1:861815/1‑251 (MQ=255)                                                                                                                                                                                      aTTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTCCCCTCCACAATTCAACAAAATAGAATTTATACCCTTAAATAAATTGCGGATAAACCGCTCTTTTTCTTTGGAAAATATCGGATTTATTGCCATTTTCAGGTAACTTTCCTTTTCGGTTTTGGCGGAAAATACGTTATAATCCCGACCAATTTTTTATCGATAAAATGACCGAGATTTCTATGt                                     <  1:323583/251‑1 (MQ=255)                                                                                                                                                                                                       attaAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTCCCCTCCACAATTCAACAAAATAGAAttt                                                                                                                                                                                                >  1:362780/1‑79 (MQ=255)                                                                                                                                                                                                         taAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTCCCCTCCACAATTCAACAAAATAGAATTTATACCCTTAAATAAATTGCGGATAAACCGCTCTTTTTCTTTGGAAAATATCGGATTTATTGCCATTTTCAGGTAACTTTCCTTTTCGGTTTTGGCGGAAAATACGTTATAATCCCGACCAATTTTTTATCGATAAAATGACCGAGATTTCTATGTTAAAAAAAGTGACCGAAAg                  <  1:361900/251‑1 (MQ=255)                                                                                                                                                                                                                   gTTAAAAAACAAATTAAAAATTCACCGTACTTTTTATTCCCCTCCACAATTCAACAAAATAGAATTTATACCCTTAAATAAATTGCGGATAAACCGCTCTTTTTCTTTGGAAAATATCGGATTTATTGCCATTTTCAGGTAACTTTCCTTTTCGGTTTTGGCGGAAAATACGTTATAATCCCGACCAATTTTTTATCGATAAAATGACCGAGATTTCTATGTTAAAAAAAGTGACCGAAAGTTTATTGGTg        >  1:879099/1‑251 (MQ=255)                                                                                                                                                                                                                         aaaCAAATTAAAAATTCACCGTACTTTTTATTCCCCTCCACAATTCAACAAAATAGAATTTATACCCTTAAATAAATTGCGGATAAACCGCTCTTTTTCTTTGGAAAATATCGGATTTATTGCCATTTTCAGGTAACTTTCCTTTTCGGTTTTGGCGGAAAATACGTTATAATCCCGACCAATTTTTTATCGATAAAATGACCGAGATTTCTATGTTAAAAAAAGTGACCGAAAGTTTATTGGTGCAGAGc  <  1:336870/251‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                      CGGGGTGAATAAAAACAACATAGCAGATTATGCCAAGTTAGGGATAAATTTATTTATCATCTCCGCGCCTTACTATGCACCGCCGGAAGATATTAAAGTGGTGATTGAGCGGATGCAGAACAAAAACACTTAAAAAAATGACCGCAC‑TTTTTTTAAATAGTTAAAAAACACAACGCAGAAATTCTTAAAAAAAAGTTATTAAAAGTGCGGTTAAAAAACAAATTAAAAATTCACCGCACTTTTTATTCCCCTCCACAATTCAACAAAATAGAATTTATACCCTTAAATAAATTGCGGATAAACCGCTCTTTTTCTTTGGAAAATATCGGATTTATTGCCATTTTCAGGTAACTTTCCTTTTCGGTTTTGGCGGAAAATACGTTATAATCCCGACCAATTTTTTATCGATAAAATGACCGAGATTTCTATGTTAAAAAAAGTGACCGAAAGTTTATTGGTGCAGAGC  >  1/757466‑757931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑