Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	752,617	T→C	F248F (TTT→TTC)	unknown →	Multidrug efflux transporter MdtK/NorM (MATE family)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	752,617	0	T	C	100.0%	206.7 / NA	74	F248F (TTT→TTC)	unknown	Multidrug efflux transporter MdtK/NorM (MATE family)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/34);  total (40/34)

GATCACCTTTCTCGGCTTGCTGATCAATATTCCTCTTAATTATATTTTTATTTATGGCAAATTGGGTGTGTCGGCATTCGGCGCCGTGGGCTGTGGCATTGCCACGGCAATTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTTCCCATTGCCATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCGGTTCTTTTGTATTTATGTTGCCTATGTCTCTCGGCATGGCAACGACCATTTTGGTGGGGCAGCGTTTGGGTGAAAAATCCCCGGAAGGCGCAAAGCAGGTGACCTATTCTGCGCTGGTTATGG  >  1/752371‑752852                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            gATCACCTTTCTCGGCTTGCTGATCAATATTCCTCTTAATTATATTTTTATTTACGGCAAATTGGGTGTGCCGGCATTCGGCGCCGTGGGCTGTGGCATTGCCACGGCAATTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCa                                                                                                                                                                                                                                         >  1:291954/1‑251 (MQ=255)     aCCTTTCTCGGCTTGCTGATCAATATTCCTCTTAATTATATTTTTATTTACGGCAAATTGGGTGTGCCGGCATTCGGCGCCGTGGGCTGTGGCATTGCCACGGCAATTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGc                                                                                                                                                                                                                                     <  1:461352/251‑1 (MQ=255)      ccTTTCTCGGCTTGCTGATCAATATTCCTCTTAATTATATTTTTATTTACGGCAAATTGGGTGTGCCGGCATTCGGCGCCGTGGGCTGTGGCATTGCCACGGCAATTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTg                                                                                                                                                                                                                                      >  1:163974/1‑249 (MQ=255)               gCTTGCTGATCAATATTCCTCTTAATTATATTTTTATTTACGGCAAATTGGGTGTGCCGGCATTCGGCGCCGTGGGCTGTGGCATTGCCACGGCAATTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTtg                                                                                                                                                                                                                           >  1:371/1‑251 (MQ=255)                 ttGCTGATCAATATTCCTCTTAATTATATTTTTATTTACGGCAAATTGGGTGTGCCGGCATTCGGCGCCGTGGGCTGTGGCATTGCCACGGCAATTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGc                                                                                                                                                                                                                                     >  1:320600/1‑239 (MQ=255)                      gATCAATATTCCTCTTAATTATATTTTTATTTACGGCAAATTGGGTGTGCCGGCATTCGGCGCCGTGGGCTGTGGCATTGCCACGGCAATTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAGATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCtgtg                                                                                                                                                                                                                    >  1:423713/1‑251 (MQ=255)                        tCAATATTCCTCTTAATTATATTTTTATTTACGGCAAATTGGGTGTGCCGGCATTCGGCGCCGTGGGCTGTGGCATTGCCACGGCAATTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGaa                                                                                                                                                                                                                  >  1:74295/1‑251 (MQ=255)                                ccTCTTAATTATATTTTTATTTACGGCAAATTGGGTGTGCCGGCATTCGGCGCCGTGGGCTGTGGCATTGCCACGGCAATTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCg                                                                                                                                                                                                                              >  1:266794/1‑231 (MQ=255)                                ccTCTTAATTATATTTTTATTTACGGCAAATTGGGTGTGCCGGCATTCGGCGCCGTGGGCTGTGGCATTGCCACGGCAATTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGc                                                                                                                                                                                                                        <  1:200338/237‑1 (MQ=255)                                   ctTAATTATATTTTTATTTCCGGCAAATGTGGTTTGCCGGCTTTCGGCGCCGTGGGCTGTGGCATTGCCACGGCAATTGTAAACTGGGTGATGTTTCTGTTGATGTTGGCATCCTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAg                                                                                                                                                                                                                 <  1:238474/241‑1 (MQ=255)                                     tAATTATATTTTTATTTACGGCAAATTGGGTGTGCCGGCATTCGGCGCCGTGGGCTGTGGCATTGCCACGGCAATTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGct                                                                                                                                                                                                                                    <  1:355430/220‑2 (MQ=255)                                                   tttacGGCAAATTGGGTGTGCCGGCATTCGGCGCCGTGGGCTGTGGCATTGCCACGGCAATTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTa                                                                                                                                                                                       <  1:326364/251‑1 (MQ=255)                                                      acggCAAATTGGGTGTGCCGGCATTCGGCGCCGTGGGCTGTGGCATTGCCACGGCAATTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTc                                                                                                                                                                                    <  1:746887/249‑1 (MQ=255)                                                       cggCAAATTGGGTGTGCCGGCATTCGGCGCCGTGGGCTGTGGCATTGCCACGGCAATTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTAtt                                                                                                                                                                                     >  1:860582/2‑249 (MQ=255)                                                         gCAAATTGGGTGTGCCGGCATTCGGCGCCGTGGGCTGTGGCATTGCCACGGCAATTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTa                                                                                                                                                                                 >  1:893695/1‑251 (MQ=255)                                                         gCAAATTGGGTGTGCCGGCATTCGGCGCCGTGGGCTGTGGCATTGCCACGGCAATTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGT‑ATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTc                                                                                                                                                                                    <  1:409825/247‑1 (MQ=255)                                                           aaaTTGGGTGTGCCGGCATTCGGCGCCGTGGGCTGTGGCATTGCCACGGCAATTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCttt                                                                                                                                                                                        <  1:370043/242‑1 (MQ=255)                                                            aaTTGGGTGTGCCGGCATTCGGCGCCGTGGGCTGTGGCATTGCCACGGCAATTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCg                                                                                                                                                                              >  1:848353/1‑251 (MQ=255)                                                                gggTGTGCCGGCATTCGGCGCCGTGGGCTGTGGCATTGCCACGGCAATTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCtt                                                                                                                                                                          >  1:638441/1‑251 (MQ=255)                                                                    gtgccGGCATTCGGCGCCGTGGGCTGTGGCATTGCCACGGCAATTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATgg                                                                                                                                                                                                                                <  1:98041/189‑1 (MQ=255)                                                                    gtgccGGCATTCGGCGCCGTGGGCTGTGGCATTGCCACGGCAATTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTggg                                                                                                                                                                      >  1:378727/5‑251 (MQ=255)                                                                     tgccGGCATTCGGCGCCGTGGGCTGTGGCATTGCCACGGCAATTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGgc                                                                                                                                                                     <  1:149423/248‑1 (MQ=255)                                                                                    ccGTGGGCTGTGGCATTGCCACGGCAATTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGc                                                                                                                                                                                                             <  1:262973/196‑1 (MQ=255)                                                                                         ggCTGTGGCATTGCCACGGCAATTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATgg                                                                                                                                                                                                                                >  1:355346/1‑172 (MQ=255)                                                                                          gCTGTGGCATTGCCACGGCAATTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAg                                                                                                                                                                                                              >  1:527707/1‑189 (MQ=255)                                                                                               ggCATTGCCACGGCAATTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCATATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAAt                                                                                                                                           >  1:288691/1‑251 (MQ=255)                                                                                               ggCATTGCCACGGCAATTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTggg                                                                                                                                                                      >  1:309605/1‑224 (MQ=255)                                                                                                         cGGCAATTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAAt                                                                                                                                 <  1:140124/251‑1 (MQ=255)                                                                                                              aTTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGa                                                                                                                                                                                                                   <  1:596270/164‑1 (MQ=255)                                                                                                              aTTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCgg                                                                                                                            <  1:883458/251‑1 (MQ=255)                                                                                                                  tAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCGGTTc                                                                                                                         <  1:799672/250‑1 (MQ=255)                                                                                                                        gggTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGct                                                                                                                                                                                                                                    >  1:285075/1‑136 (MQ=255)                                                                                                                        gggTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCg                                                                                                                                                                                                                              >  1:92201/1‑143 (MQ=255)                                                                                                                          gTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCGGTTCTTTTGTAt                                                                                                                 <  1:97828/250‑1 (MQ=255)                                                                                                                            gATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCGGTTCTTTTGTATTTa                                                                                                              >  1:490572/1‑251 (MQ=255)                                                                                                                              tgtgTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCGGTTCTTTTGTATTTATg                                                                                                            <  1:636237/251‑1 (MQ=255)                                                                                                                                   cTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGTGTTGTGCTGTGAAGTAGCATTGTTCGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCGGTTCTTTTGTATTTATGTTGc                                                                                                        >  1:648681/1‑250 (MQ=255)                                                                                                                                   cTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCGGTTCTTTTGTATTTATGTTGc                                                                                                        >  1:489722/1‑250 (MQ=255)                                                                                                                                     gTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCGGTTCTTTTGTATTTATGTTGCCTa                                                                                                     >  1:669520/1‑251 (MQ=255)                                                                                                                                     gTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCGGTTCTTTTGTATTTATGTTGCCTa                                                                                                     >  1:718372/1‑251 (MQ=255)                                                                                                                                         aTGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCGGTTCTTTTGTATTTATGTTGCCTATGtc                                                                                                 <  1:3069/251‑1 (MQ=255)                                                                                                                                          tGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATAc                                                                                                                               >  1:712860/1‑220 (MQ=255)                                                                                                                                           gTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAAcc                                                                                                                                                                                             >  1:854741/1‑157 (MQ=255)                                                                                                                                           gTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCGGTTCTTTTGTATTTATGTTGCCTATGtct                                                                                                >  1:709561/1‑250 (MQ=255)                                                                                                                                           gTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCGGTTCTTTTGTATTTATGTTGCCTATGtct                                                                                                >  1:406082/1‑250 (MQ=255)                                                                                                                                              ggCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCt                                                                                                                                                                                                 >  1:639422/1‑150 (MQ=255)                                                                                                                                              ggCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCa                                                                                                                                                      >  1:585232/1‑193 (MQ=255)                                                                                                                                              ggCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCGGTTCTTTTGTATTTATg                                                                                                            <  1:11524/235‑1 (MQ=255)                                                                                                                                                cATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCGGTTCTTTTGTATTTATGTTGCCTATGTCTCTCGGc                                                                                          <  1:677921/251‑1 (MQ=255)                                                                                                                                                cATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATAGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCGGTTCTTTTGTATTTATGTTGCCTATGTCTCTCgg                                                                                           <  1:474731/250‑1 (MQ=255)                                                                                                                                                   aCTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGc                                                                                                                                                                                                                                     <  1:339860/109‑1 (MQ=255)                                                                                                                                                   aCTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCt                                                                                                                                                                           >  1:537091/1‑167 (MQ=255)                                                                                                                                                   aCTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCGGTTCTTTTGTAt                                                                                                                 <  1:352775/225‑1 (MQ=255)                                                                                                                                                   aCTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCGGTTCTTTTGTATTTATGTTGCCTATGtc                                                                                                 >  1:45276/1‑241 (MQ=255)                                                                                                                                                   aCTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCGGTTCTTTTGTATTTATGTTGCCTATGTCTCTCGGCAt                                                                                        <  1:749096/250‑1 (MQ=255)                                                                                                                                                    cTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTAt                                                                                                                                                                                      <  1:167458/155‑1 (MQ=255)                                                                                                                                                    cTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTc                                                                                                                                                                                    <  1:765596/157‑1 (MQ=255)                                                                                                                                                      gTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCAt                                                                                                                                                                                                           >  1:141198/1‑132 (MQ=255)                                                                                                                                                      gTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGc                                                                                                                                                       <  1:727937/184‑1 (MQ=255)                                                                                                                                                      gTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCGGTTCTTTTGTATTTATGTTGCCTATGTCTCTCGGCATGGCa                                                                                    >  1:101833/1‑251 (MQ=255)                                                                                                                                                      gTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCGGTTCTTTTGTATTTATGTTGCCTATGTCTCTCGGCATGGCa                                                                                    >  1:206304/1‑251 (MQ=255)                                                                                                                                                      gTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCGGTTCTTTTGTATTTATGTTGCCTATGTCTCTCGGCATGGCa                                                                                    >  1:347526/1‑251 (MQ=255)                                                                                                                                                                   aaaTCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGc                                                                                                                                                                                                                        <  1:753099/106‑1 (MQ=255)                                                                                                                                                                    aaTCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCGGTTCTTTTGTATTTATGTTGCCTATGTCTCTCGGCATGGCAACGACCATTTtggt                                                                      <  1:201923/251‑1 (MQ=255)                                                                                                                                                                              aTTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCgg                                                                                                                            <  1:1624/187‑1 (MQ=255)                                                                                                                                                                                      gTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTggg                                                                                                                                                                      <  1:111962/137‑1 (MQ=255)                                                                                                                                                                                                    cATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATAc                                                                                                                               <  1:290715/162‑1 (MQ=255)                                                                                                                                                                                                      ttGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCGGTTCTTTTGTATTTATGTTGCCTATGTCTCTCGGCATGGCAACGACCATTTTGGTGGGGCAGCGTTTGGGTGAAAAATCCCCGGAAgg                                     >  1:449352/1‑250 (MQ=255)                                                                                                                                                                                                                gAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCGGTTCTTTTGTATTTATGTTGCCTATGTCTCTCGGCATGGCAACGACCATTTTGGTGGGGCAGCGTTTGGGTGAAAAATCCCCGGAAGGCGCAAAGCAgg                          >  1:628005/1‑251 (MQ=255)                                                                                                                                                                                                                     gACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCGGTTCTTTTGTATTTATGTTGCCTATGTCTCTCGGCATGGCAACGACCATTTTGGTGGGGCAGCGTTTGGGTGaaaaa                                                >  1:515004/1‑224 (MQ=255)                                                                                                                                                                                                                      acgcacgcTGGGGAAATTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCGGTTCTTTTGTATTTATGTTGCCTATGTCTCTCGGCATGGCAACGACCATTTTGGTGGGGCAGCGTTTGGGTGAAAAATCCCCGGAAGGCGCAAAGCAGGTGAc                      <  1:178591/249‑1 (MQ=255)                                                                                                                                                                                                                                    aaTTGCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCGGTTCTTTTGTATTTATGTTGCCTATGTCTCTCGGCATGGCAACGACCATTTTGGTGGGGCAGCGTTTGGGTGAAAAATCCCCGGAAGGCGCAAAGCAGGTGACCTATTCTGCGCTGGtt      <  1:69242/251‑1 (MQ=255)                                                                                                                                                                                                                                        gCTGAAGCTGGGTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCGGTTCTTTTGTATTTATGTTGCCTATGTCTCTCGGCATGGCAACGACCATTTTGGTGGGGCAGCGTTTGGGTGAAAAATCCCCGGAAGGCGCAAAGCAGGTGACCTATTCTGCGCTGGTTATgg  >  1:124461/1‑251 (MQ=255)                                                                                                                                                                                                                                                 gggTTTCCCCATTGCTATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTAt                                                                                                                                                                                      >  1:416905/1‑62 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            GATCACCTTTCTCGGCTTGCTGATCAATATTCCTCTTAATTATATTTTTATTTATGGCAAATTGGGTGTGTCGGCATTCGGCGCCGTGGGCTGTGGCATTGCCACGGCAATTGTAAACTGGGTGATGTGTCTGTTGATGTTGGCATACTGTATGCGGGCGAAAAATCAACGTGATTTGAAGGTTTTCGCAAATATCATTGAACGCCCGAATAGACGCACGCTGGGGAAATTGCTGAAGCTGGGTTTTCCCATTGCCATGGCGTTGTGCTGTGAAGTAGCATTGTTTGCGCTAACCTCTTTATTCTTATCGCCTTTGGGCGCCGACGTGGTGGCAAGTCATCAAATTGCACTAAATACCGGTTCTTTTGTATTTATGTTGCCTATGTCTCTCGGCATGGCAACGACCATTTTGGTGGGGCAGCGTTTGGGTGAAAAATCCCCGGAAGGCGCAAAGCAGGTGACCTATTCTGCGCTGGTTATGG  >  1/752371‑752852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑