Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	750,570	G→A	intergenic (‑130/‑38)	unknown ← / → unknown	RNA‑binding protein YhbY/Phage protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	750,570	0	G	A	100.0%	205.9 / NA	77	intergenic (‑130/‑38)	unknown/unknown	RNA‑binding protein YhbY/Phage protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/34);  total (43/34)

TTTCTGCCAGCACGCCTTCCGTTAAACCATTGCCACCGAGCATCACCACCGGGCTTAAATGATGGGCCAGTCCTTTTAGATACTGTTTTTGTTTGGTTGATAATGTAATTGACATAATAATCTCTTTGAAAAAATTGATGGCTGATTCTAATGCAATATTTATTTCGATACAACATATCGGGTGAAAGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGGCTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGATTGATAAAGGTCAGGGGTATCGGGTTTATCTGAAAAATCAAAATGG  >  1/750326‑750813                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    tttCTGCCAGCACGCCTTCCGTTAAACCATTGCCACCGAGCATCACCACCGGGCTTAAATGATGGGCCAGTCCTTTTAGATACTGTTTTTGTTTGGTTGATAATGTAATTGACATAATAATCTCTTTGAAAAAATTGATGGCTGATTCTAATGCAATATTTATTTCGATACAACATATCGGGTGAAAGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATAc                                                                                                                                                                                                                                               <  1:124638/251‑1 (MQ=255)    cTGCCAGCACGCCTTCCGTTAAACCATTGCCACCGAGCATCACCACCGGGCTTAAATGATGGGCCAGTCCTTTTAGATACTGTTTTTGTTTGGTTGATAATGTAATTGACATAATAATCTCTTTGAAAAAATTGATGGCTGATTCTAATGCAATATTTATTTCGATACAACATATCGGGTGAAAGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTa                                                                                                                                                                                                                                            <  1:875394/251‑1 (MQ=255)    cTGCCAGCACGCCTTCCGTTAAACCATTGCCACCGAGCATCACCACCGGGCTTAAATGATGGGCCAGTCCTTTTAGATACTGTTTTTGTTTGGTTGATAATGTAATTGACATAATAATCTCTTTGAAAAAATTGATGGCTGATTCTAATGCAATATTTATTTCGATACAACATATCGGGTGAAAGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTa                                                                                                                                                                                                                                            <  1:766150/251‑1 (MQ=255)    cTGCCAGCACGCCTTCCGTTAAACCATTGCCACCGAGCATCACAACCGGGCTTAAATGATGGGCCAGTACTTTTAGATACTGTTTTTGTTTGGTTGATAATGTAATTGACATAATAAGCTCTTTGAAAAAATTGATGGGTGATTCTAATGAAATATTTATTTCGATACAACATATCGGGTGAAAGAACCTTAGGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTa                                                                                                                                                                                                                                            <  1:781821/251‑1 (MQ=255)                      ttAAACCATTGCCACCGAGCATCACCACCGGGCTTAAATGATGGGCCAGTCCTTTTAGATACTGTTTTTGTTTGGTTGATAATGTAATTGACATAATAATCTCTTTGAAAAAATTGATGGCTGATTCTAATGCAATATTTATTTCGATACAACATATCGGGTGAAAGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGAc                                                                                                                                                                                                                          <  1:32910/251‑1 (MQ=255)                                       aGCATCACCACCGGGCTTAAATGATGGGCCAGTCCTTTTAGATACTGTTTTTGTTTGGTTGATAATGTAATTGACATAATAATCTCTTTGAAAAAATTGATGGCTGATTCTAATGCAATATTTATTTCGATACAACATATCGGGTGAAAGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAatga                                                                                                                                                                                                            >  1:179696/1‑248 (MQ=255)                                                    ggCTTAAATGATGGGCCAGTCCTTTTAGATACTGTTTTTGTTTGGTTGATAATGTAATTGACATAATAATCTCTTTGAAAAAATTGATGGCTGATTCTAATGCAATATTTATTTCGATACAACATATCGGGTGAAAGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACt                                                                                                                                                                                                                                              <  1:547019/201‑1 (MQ=255)                                                     gCTTAAATGATGGGCCAGTCCTTTTAGATACTGTTTTTGTTTGGTTGATAATGTAATTGACATAATAATCTCTTTGAAAAAATTGATGGCTGATTCTAATGCAATATTTATTTCGATACAACATATCGGGTGAAAGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAgacg                                                                                                                                                                                           >  1:450342/1‑251 (MQ=255)                                                      cTTAAATGATGGGCCAGTCCTTTTAGATACTGTTTTTGTTTGGTTGATAATGTAATTGACATAATAATCTCTTTGAAAAAATTGATGGCTGATTCTAATGCAATATTTATTTCGATACAACATATCGGGTGAAAGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAgacga                                                                                                                                                                                          >  1:575449/1‑251 (MQ=255)                                                                         cTTTTAGATACTGTTTTTGTTTGGTTGATAATGTAATTGACATAATAATCTCTTTGAAAAAATTGATGGCTGATTCTAATGCAATATTTATTTCGATACAACATATCGGGTGAAAGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATAc                                                                                                                                                                                                                                               >  1:780595/1‑179 (MQ=255)                                                                                     gTTTTTGTTTGGTTGATAATGTAATTGACATAATAATCTCTTTGAAAAAATTGATGGCTGATTCTAATGCAATATTTATTTCGATACAACATATCGGGTGAAAGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTgaagaa                                                                                                                                                              >  1:339307/1‑248 (MQ=255)                                                                                                gTTGATAATGTAATTGACATAATAATCTCTTTGAAAAAATTGATGGCTGATTCTAATGCAATATTTATTTCGATACAACATATCGGGTGAAAGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACtt                                                                                                                                                                                                                                             <  1:353603/158‑1 (MQ=255)                                                                                                       aTGTAATTGACATAATAATCTCTTTGAAAAAATTGATGGCTGATTCTAATGCAATATTTATTTCGATACAACATATCGGGTGAAAGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCg                                                                                                                                                                                                                                        <  1:436830/156‑1 (MQ=255)                                                                                                         gTAATTGACATAATAATCTCTTTGAAAAAATTGATGGCTGATTCTAATGCAATATTTATTTCGATACAACATATCGGGTGAAAGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAg                                                                                                                                       >  1:458904/1‑251 (MQ=255)                                                                                                                aCATAATAATCTCTTTGAAAAAATTGATGGCTGATTCTAATGCAATATTTATTTCGATACAACATATCGGGTGAAAGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAAt                                                                                                                                >  1:488139/1‑251 (MQ=255)                                                                                                                 cATAATAATCTCTTTGAAAAAATTGATGGCTGATTCTAATGCAATATTTATTTCGATACAACATATCGGGTGAAAGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGActtttctttt                                                                                                                                                                                >  1:333642/1‑202 (MQ=255)                                                                                                                 cATAATAATCTCTTTGAAAAAATTGATGGCTGATTCTAATGCAATATTTATTTCGATACAACATATCGGGTGAAAGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAAt                                                                                                                                >  1:56905/1‑250 (MQ=255)                                                                                                                                                  ttCTAATGCAATATTTATTTCGATACAACATATCGGGTGAAAGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACaa                                                                                                                                                                                                    >  1:613844/1‑149 (MQ=255)                                                                                                                                                  ttCTAATGCAATATTTATTTCGATACAACATATCGGGTGAAAGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAAt                                                                                              >  1:833048/1‑251 (MQ=255)                                                                                                                                                             tatttattTCGATACAACATATCGGGTGAAAGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGAttt                                                                                   >  1:738106/1‑251 (MQ=255)                                                                                                                                                                 tatttCGATACAACATATCGGGTGAAAGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTaaaa                                                                               <  1:491366/251‑1 (MQ=255)                                                                                                                                                                      cGATACAACATATCGGGTGAAAGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGt                                                                          >  1:748170/1‑251 (MQ=255)                                                                                                                                                                           caacaTATCGGGTGAAAGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATg                                                                     >  1:171570/1‑251 (MQ=255)                                                                                                                                                                            aacaTATCGGGTGAAAGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGa                                                                    >  1:394093/1‑251 (MQ=255)                                                                                                                                                                              caTATCGGGTGAAAGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACg                                                                  >  1:409393/1‑251 (MQ=255)                                                                                                                                                                                       tGAAAGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTg                                                         <  1:432122/251‑1 (MQ=255)                                                                                                                                                                                        gAAAGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATAc                                                                                                                                                                                                                                               <  1:536890/68‑1 (MQ=255)                                                                                                                                                                                         aaaGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGtt                                                                                                     <  1:818104/205‑1 (MQ=255)                                                                                                                                                                                          aaGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGaaa                                                      <  1:365429/251‑1 (MQ=255)                                                                                                                                                                                             aaCCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATg                                                    >  1:724173/1‑250 (MQ=255)                                                                                                                                                                                              aCCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTaa                                                                                               >  1:30377/1‑206 (MQ=255)                                                                                                                                                                                              aCCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGaaa                                                      <  1:228/247‑1 (MQ=255)                                                                                                                                                                                              aCCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCg                                                  >  1:635098/1‑251 (MQ=255)                                                                                                                                                                                              aCCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCg                                                  >  1:481325/1‑251 (MQ=255)                                                                                                                                                                                                   aTGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTc                                                                                                                                                                                                                 <  1:500517/87‑1 (MQ=255)                                                                                                                                                                                                        gATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAAcc                                                                                                                                                   >  1:362200/1‑144 (MQ=255)                                                                                                                                                                                                        gATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGATTGATaaa                                        >  1:506051/1‑251 (MQ=255)                                                                                                                                                                                                           aaaGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAA‑ACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGATTGATAAAGGTc                                    <  1:659592/251‑1 (MQ=255)                                                                                                                                                                                                             aGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTtgat                                                                                                                                                                            <  1:324005/114‑1 (MQ=255)                                                                                                                                                                                                              ggTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTc                                                                                           <  1:125315/194‑1 (MQ=255)                                                                                                                                                                                                              ggTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGaaa                                                      <  1:775612/231‑1 (MQ=255)                                                                                                                                                                                                              ggTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGATTGATaa                                         <  1:208618/244‑1 (MQ=255)                                                                                                                                                                                                              ggTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGATTGATAAAGGTCAg                                  >  1:723033/1‑251 (MQ=255)                                                                                                                                                                                                                 aaaGTGTTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGATTGATAAAGGTCAgggg                               <  1:580487/251‑1 (MQ=255)                                                                                                                                                                                                                    gtgtTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGATTGATAAAGGt                                     >  1:275192/1‑242 (MQ=255)                                                                                                                                                                                                                    gtgtTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGATTGATAAAGGt                                     <  1:59671/242‑1 (MQ=255)                                                                                                                                                                                                                      gtTAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGc                                                                                                                          >  1:480218/1‑155 (MQ=255)                                                                                                                                                                                                                        tAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGc                                                                                                                                            <  1:401618/135‑1 (MQ=255)                                                                                                                                                                                                                        tAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGc                                                                                                                                            >  1:182500/1‑135 (MQ=255)                                                                                                                                                                                                                        tAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGATTGATAAAGGTCAGGGGTATCggg                        <  1:755407/251‑1 (MQ=255)                                                                                                                                                                                                                        tAACTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGATTGATAAAGGTCAGGGGTATCggg                        >  1:325950/1‑251 (MQ=255)                                                                                                                                                                                                                         aaCTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTaaa                                                                                                                >  1:793360/1‑162 (MQ=255)                                                                                                                                                                                                                          aCTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTaa                                                                                               >  1:799176/1‑178 (MQ=255)                                                                                                                                                                                                                           cTATACTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGATTGATAAAGGTCAGGGGTATCGGGttt                     >  1:678314/1‑251 (MQ=255)                                                                                                                                                                                                                              taCTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTg                                                         <  1:770951/212‑1 (MQ=255)                                                                                                                                                                                                                               aCTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGATTGATAAAGGTCAGGGGTATCGGGTTTAt                   >  1:646450/1‑249 (MQ=255)                                                                                                                                                                                                                               aCTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGATTGATAAAGGTCAGGGGTATCGGGTTTAt                   >  1:537274/1‑249 (MQ=255)                                                                                                                                                                                                                               aCTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGATTGATAAAGGTCAGGGGTATCGGGTTTAt                   >  1:308245/1‑249 (MQ=255)                                                                                                                                                                                                                               aCTTGACACTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGATTGATAAAGGTCAGGGGTATCGGGTTTATCt                 <  1:530138/251‑1 (MQ=255)                                                                                                                                                                                                                                    acacTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGATTGATAAAGGTCAGGGGTATCGGGTTTATCTGaaa             <  1:706514/250‑1 (MQ=255)                                                                                                                                                                                                                                       cTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCATGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGATTGATaa                                         <  1:107439/219‑1 (MQ=255)                                                                                                                                                                                                                                       cTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAAttt                                                                                            >  1:444574/1‑168 (MQ=255)                                                                                                                                                                                                                                       cTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTaaaa                                                                               >  1:103116/1‑181 (MQ=255)                                                                                                                                                                                                                                       cTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGATTGATaa                                         <  1:160799/219‑1 (MQ=255)                                                                                                                                                                                                                                       cTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGATTGATAAAGGTCAgggg                               <  1:810091/229‑1 (MQ=255)                                                                                                                                                                                                                                       cTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGATTGATAAAGGTCAGGGGTATCGGGttt                     <  1:571546/239‑1 (MQ=255)                                                                                                                                                                                                                                       cTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGATTGATAAAGGTCAGGGGTATCGGGTTTATCTGAAAAAt          >  1:664107/1‑250 (MQ=255)                                                                                                                                                                                                                                       cTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGATTGATAAAGGTCAGGGGTATCGGGTTTATCTGAAAAAt          >  1:320977/1‑250 (MQ=255)                                                                                                                                                                                                                                       cTTTTAAGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGATTGATAAAGGTCAGGGGTATCGGGTTTATCTGAAAAAt          >  1:310862/1‑250 (MQ=255)                                                                                                                                                                                                                                            aaGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGATTGATAAAGGTCAgggg                               >  1:315411/1‑224 (MQ=255)                                                                                                                                                                                                                                            aaGCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGATTGATAAAGGTCAGGGGTATCGGGTTTATCTGAAAAATCAAAAt    >  1:689979/1‑251 (MQ=255)                                                                                                                                                                                                                                              gCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTc                                                                                                                                                                                                                 >  1:499994/1‑44 (MQ=255)                                                                                                                                                                                                                                              gCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAg                                                                             <  1:368220/176‑1 (MQ=255)                                                                                                                                                                                                                                              gCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGATTGATAAAGGTCAGGGGTATCGGGTTTATCTGAAAAATCAAAATgg  >  1:870104/1‑251 (MQ=255)                                                                                                                                                                                                                                              gCTAAAGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGATTGATAAAGGTCAGGGGTATCGGGTTTATCTGAAAAATCAAAATgg  >  1:92538/1‑251 (MQ=255)                                                                                                                                                                                                                                                 aaaGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAg                                                                                                       <  1:371943/147‑1 (MQ=255)                                                                                                                                                                                                                                                 aaaGACTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGATTGATAAAGGTCAgggg                               <  1:734516/219‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    TTTCTGCCAGCACGCCTTCCGTTAAACCATTGCCACCGAGCATCACCACCGGGCTTAAATGATGGGCCAGTCCTTTTAGATACTGTTTTTGTTTGGTTGATAATGTAATTGACATAATAATCTCTTTGAAAAAATTGATGGCTGATTCTAATGCAATATTTATTTCGATACAACATATCGGGTGAAAGAACCTTATGTTGATAAAGGTAAAGTGTTAACTATACTTGACACTTTTAAGCTAAAGGCTATACTTAGGCGAAACAAATAACGACAATTAATTCAATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAATCAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGATTGATAAAGGTCAGGGGTATCGGGTTTATCTGAAAAATCAAAATGG  >  1/750326‑750813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑