Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	749,183	T→C	T83A (ACG→GCG)	unknown ←	D‑alanyl‑D‑alanine carboxypeptidase (EC 3.4.16.4)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	749,183	0	T	C	100.0%	196.0 / NA	69	T83A (ACG→GCG)	unknown	D‑alanyl‑D‑alanine carboxypeptidase (EC 3.4.16.4)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/41);  total (28/41)

GATCATTCCAGATCCAGCCTAAACCGCGATCGTGACCGATAAACACCGACGTATCTAAAATCAGATTGCCGGTGATTTTATTAATGTTTTGCTTCTTTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGTCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAATTTGCCCCCTTGGGAAGATTGGCGGTGTAATTGGCAATATTGACTTCGGCGAAAGAGGCGGTGGCGATGCCGAGGCTGAGTAATAAGAATGTTTCTTGAAAAATATGACGTGGTGAAAATTTATAC  >  1/748940‑749427                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     gATCATTCCAGATCCAGCCTAAACCGCGATCGTGACCGATAAACACCGACGTATCTAAAATCAGATTGCCGGTGATTTTATTAATGTTTTGCTTCTTTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAAtttt                                                                                                                                                                                                                                               >  1:513674/1‑251 (MQ=255)      ttCCAGATCCAGCCTAAACCGCGATCGTGACCGACAAACACCGACGTATCTAAAATCAGATTGCCGGTGATTTTATTAATGTTTTGCTTCTTTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTgccg                                                                                                                                                                                                                                           >  1:117677/1‑250 (MQ=255)                 gCCTAAACCGCGATCGTGACCGATAAACACCGACGTATCTAAAATCAGATTGCCGGTGATTTTATTAATGTTTTGCTTCTTTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTaa                                                                                                                                                                                                                               >  1:852874/1‑251 (MQ=255)                     aaaCCGCGATCGTGACCGATAAACACCGACGTATCTAAAATCAGATTGCCGGTGATTTTATTAATGTTTTGCTTCTTTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATa                                                                                                                                                                                                                            >  1:901909/1‑250 (MQ=255)                       aCCGCGATCGTGACCGATAAACACCGACGTATCTAAAATCAGATTGCCGGTGATTTTATTAATGTTTTGCTTCTTTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACtt                                                                                                                                                                                                                         <  1:894041/251‑1 (MQ=255)                            gATCGTGACCGATAAACACCGACGTATCTAAAATCAGATTGCCGGTGATTTTATTAATGTTTTGCTTCTTTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTg                                                                                                                                                                                                                     >  1:842632/1‑250 (MQ=255)                                 tGACCGATAAACACCGACGTATCTAAAATCAGATTGCCGGTGATTTTATTAATGTTTTGCTTCTTTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTg                                                                                                                                                                                                                 >  1:252329/1‑249 (MQ=255)                                      gATAAACACCGACGTATCTAAAATCAGATTGCCGGTGATTTTATTAATGTTTTGCTTCTTTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCgg                                                                                                                                                                                                          >  1:637516/1‑251 (MQ=255)                                                 aCGTATCTAAAATCAGATTGCCGGTGATTTTATTAATGTTTTGCTTCTTTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGaa                                                                                                                                                                                                <  1:172440/250‑1 (MQ=255)                                                  cGTATCTAAAATCAGATTGCCGGTGATTTTATTAATGTTTTGCTTCTTTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACg                                                                                                                                                                                              <  1:761160/251‑1 (MQ=255)                                                  cGTATCTAAAATCAGATTGCCGGTGATTTTATTAATGTTTTGCTTCTTTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACg                                                                                                                                                                                              <  1:391064/251‑1 (MQ=255)                                                      tCTAAAATCAGATTGCCGGTGATTTTATTAATGTTTTGCTTCTTTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTg                                                                                                                                                                                          >  1:271952/1‑251 (MQ=255)                                                         aaaaTCAGATTGCCGGTGATTTTATTAATGTTTTGCTTCTTTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTcc                                                                                                                                                                                       <  1:305649/251‑1 (MQ=255)                                                                 aTTGCCGGTGATTTTATTAATGTTTTGCTTCTTTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAAt                                                                                                                                                                               <  1:111044/251‑1 (MQ=255)                                                                  ttGCCGGTGATTTTATTAATGTTTTGCTTCTTTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATc                                                                                                                                                                              <  1:727466/251‑1 (MQ=255)                                                                            ttttATTAATGTTTTGCTTCTTTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATg                                                                                                                                                                    <  1:748532/251‑1 (MQ=255)                                                                            ttttATTAATGTTTTGCTTCTTTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATg                                                                                                                                                                    <  1:77092/251‑1 (MQ=255)                                                                            ttttATTAATGTTTTGCTTCTTTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATg                                                                                                                                                                    <  1:530109/251‑1 (MQ=255)                                                                            ttttATTAATGTTTTGCTTCTTTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATg                                                                                                                                                                    <  1:42044/251‑1 (MQ=255)                                                                              ttattaATGTTTTGCTTCTTTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGt                                                                                                                                                                                             >  1:562719/1‑224 (MQ=255)                                                                              ttattaATGTTTTGCTTCTTTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGtt                                                                                                                                                                  >  1:405677/1‑251 (MQ=255)                                                                               tattaATGTTTTGCTTCTTTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTa                                                                                                                                                                 <  1:4084/251‑1 (MQ=255)                                                                                  taATGTTTTGCTTCTTTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCt                                                                                                                                                              <  1:759815/251‑1 (MQ=255)                                                                                  taATGTTTTGCTTCTTTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAAGTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCt                                                                                                                                                              <  1:440691/251‑1 (MQ=255)                                                                                    aTGTTTTGCTTCTTTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCttg                                                                                                                                                            <  1:685824/251‑1 (MQ=255)                                                                                               cttTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAAttt                                                                                                                                                                                                                                                <  1:135716/156‑1 (MQ=255)                                                                                               cttTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGaa                                                                                                                                                                                                <  1:287332/204‑1 (MQ=255)                                                                                               cttTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATTATGTTATCTTGATTTATGtttt                                                                                                                                                 >  1:821751/1‑251 (MQ=255)                                                                                               cttTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCt                                                                                                                                                              <  1:749482/238‑1 (MQ=255)                                                                                                  tAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTgg                                                                                                                                              <  1:623024/251‑1 (MQ=255)                                                                                                   aaTTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGc                                                                                                                                             <  1:345897/251‑1 (MQ=255)                                                                                                      tCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATc                                                                                                                                                               <  1:118173/230‑1 (MQ=255)                                                                                                          ataataAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACg                                                                                                                                                                                              <  1:683424/195‑1 (MQ=255)                                                                                                          ataataAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGAGGTAAATACTTTTTGCGTGCTGGCGGGGGGCATGAACGTTTGTCCGTTACAATCGGCGATAATg                                                                                                                                                                    >  1:846368/1‑221 (MQ=255)                                                                                                             ataaATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTTAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGcc                                                                                                                                   <  1:655680/251‑1 (MQ=255)                                                                                                               aaaTTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCaa                                                                                                                                 >  1:801671/1‑251 (MQ=255)                                                                                                                  ttGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAAtt                                                                                                                              >  1:680146/1‑251 (MQ=255)                                                                                                                               cGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAATTTGCCCCCTTGGGa                                                                                                                 <  1:445595/251‑1 (MQ=255)                                                                                                                               cGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAATTTGCCCCCTTGGGa                                                                                                                 <  1:362264/251‑1 (MQ=255)                                                                                                                                   ggTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATAc                                                                                                                                                                                                                           >  1:741495/1‑141 (MQ=255)                                                                                                                                   ggTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAATTTGCCCCCTTGGGAAg                                                                                                               >  1:485743/1‑249 (MQ=255)                                                                                                                                   ggTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAATTTGCCCCCTTGGGAAGAt                                                                                                             >  1:780259/1‑251 (MQ=255)                                                                                                                                   ggTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAATTTGCCCCCTTGGGAAGAt                                                                                                             >  1:484324/1‑251 (MQ=255)                                                                                                                                   ggTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGGTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAATTTGccccc                                                                                                                       >  1:536800/1‑241 (MQ=255)                                                                                                                                   ggTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACATTTGTCCGTTATAATCGGCGATAATGTTATCt                                                                                                                                                              >  1:456173/1‑202 (MQ=255)                                                                                                                                      tAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAATTTGCCCCCTTGGGAAGATTgg                                                                                                          <  1:854171/251‑1 (MQ=255)                                                                                                                                      tAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAATTTGCCCCCTTGGGAAGATTgg                                                                                                          <  1:307998/251‑1 (MQ=255)                                                                                                                                        aaTCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAATTTGCCCCCTTGGGAAg                                                                                                               >  1:284538/1‑244 (MQ=255)                                                                                                                                        aaTCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAATTTGCCCCCTTGGGAAGATTGGCg                                                                                                        <  1:670457/251‑1 (MQ=255)                                                                                                                                        aaTCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAATTTGCCCCCTTGGGAAGATTGGCg                                                                                                        >  1:594313/1‑251 (MQ=255)                                                                                                                                         aTCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGTGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAATTTGCCCCCTTGGGAAGATTGGCgg                                                                                                       <  1:712219/251‑1 (MQ=255)                                                                                                                                               gTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAATTTGCCCCCTTGGGAAGATTGGCGGTGTaa                                                                                                  <  1:287172/250‑1 (MQ=255)                                                                                                                                                      gTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATTGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGt                                                                                                                                                                                             <  1:78733/152‑1 (MQ=255)                                                                                                                                                              cacaATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAATTTGCCCCCTTGGGAAGATTGGCGGTGTAATTGGCAATATTGACt                                                                                   <  1:11130/250‑1 (MQ=255)                                                                                                                                                               acaATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAATTTGCCCCCTTGGGAAGATTGGCGGTGTAATTGGCAATATTGACTTc                                                                                 <  1:397412/251‑1 (MQ=255)                                                                                                                                                                caATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGttttt                                                                                                                                                >  1:213315/1‑187 (MQ=255)                                                                                                                                                                        tCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAATTTGCCCCCTTGGGAAGATTGGCGGTGTAATTGGCAATATTGACTTCGGCTAAaga                                                                        <  1:836173/251‑1 (MQ=255)                                                                                                                                                                                         gTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAATTTGCCCCCTTGGGAAGATTGGCGGTGTAATTGGCAATATTTACTTCGGCTAAag                                                                         >  1:417647/1‑233 (MQ=255)                                                                                                                                                                                         gTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAATTTGCCCCCTTGGGAAGATTGGCGGTGTAATTGGCAATATTGACTTCGGCTAAAGAGGCggggggggagtcgg                                                       >  1:373499/1‑239 (MQ=255)                                                                                                                                                                                              gTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAATTTGccccc                                                                                                                       >  1:7018/1‑182 (MQ=255)                                                                                                                                                                                                aCTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGaa                                                                                                                                                                                                >  1:283021/1‑107 (MQ=255)                                                                                                                                                                                                      ccGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAATTTGCCCCCTTGGGAAGATTGGCGGGGTAATTGGCAATATTGACTTCGGCTAAAGAGGCgggggggaaggcggggggggggaaaaa                                          >  1:703564/1‑226 (MQ=255)                                                                                                                                                                                                         ttGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAATTTGCCCCCTTGGGAAGATTGGCGGTGTAATTGGCAATATTGACTTCGGCTAAAGAGGCGGTGGCGATGCCGAGGCTGAGTAATAAGa                                        <  1:389941/250‑1 (MQ=255)                                                                                                                                                                                                           ggTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAATTTGCCCCCTTGGGAAGATTGGCgg                                                                                                       <  1:660172/185‑1 (MQ=255)                                                                                                                                                                                                           ggTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAATTTGCCCCCTTGGGAAGATTGGCGGTGTAATTGGCAATATTGACTTCGGCTAAAGAGGCGGTGGCGATGCCGAGGCTGAGTAATAAGaa                                       <  1:585893/249‑1 (MQ=255)                                                                                                                                                                                                                      cGGTTTGAAATTGAAATGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAATTTGCCCCCTTGGGAAGATTGGCGGTGTAATTGGCAATATTGACTTCGGCTAAAGAGGCGGTGGCGATGCCGAGGCTGAGTAATAAGAATGTTTc                                 <  1:554126/244‑1 (MQ=255)                                                                                                                                                                                                                             aaattgaaatGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAATTTGCCCCCTTGGGAAGATTGGCGGTGTAATTGGCAATATTGACTTCGGCTAAAGAGGCGGTGGCGATGCCGAGGc                                                    <  1:80569/218‑1 (MQ=255)                                                                                                                                                                                                                                  gaaatGAATCGCCGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAATTTGCCCCCTTGGGAAGATTGGCGGTGTAATTGGCAATATTGACTTCGGCTAAaga                                                                        <  1:333028/193‑1 (MQ=255)                                                                                                                                                                                                                                              cGAGCGCCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAATTTGCCCCCTTGGGAAGATTGGCGGTGTAATTGGCAATATTGACTTCGGCTAAAGAGGCGGTGGCGATGCCGAGGCTGAGTAATAAGAATGTTTCTTGAAAAATATGACGTGGTGAAAATTTATAc  <  1:109958/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     GATCATTCCAGATCCAGCCTAAACCGCGATCGTGACCGATAAACACCGACGTATCTAAAATCAGATTGCCGGTGATTTTATTAATGTTTTGCTTCTTTAATTCCGATAATAAATTGAATAATTGCCCGCTGGTTAAATCCGGGTCGCCGGTAAAACGCACAATTAAATCGCCGTTGAGTTGACCGTTTTGTACTTTGCCGTTGGTTAAAAGTGCGGTTTGAAATTGAAATGAATCGCCGAGCGTCAATTTTGCCGCCAAAGCGGTAAATACTTTTTGCGTGCTGGCGGGGAGCATGAACGTTTGTCCGTTATAATCGGCGATAATGTTATCTTGATTGATGTTTTTGGCGATGAATGCCAAATTTGCCCCCTTGGGAAGATTGGCGGTGTAATTGGCAATATTGACTTCGGCGAAAGAGGCGGTGGCGATGCCGAGGCTGAGTAATAAGAATGTTTCTTGAAAAATATGACGTGGTGAAAATTTATAC  >  1/748940‑749427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑