Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	746,077	C→T	G342D (GGT→GAT)	unknown ←	Molybdopterin molybdenumtransferase (EC 2.10.1.1)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	746,077	0	C	T	100.0%	193.8 / NA	72	G342D (GGT→GAT)	unknown	Molybdopterin molybdenumtransferase (EC 2.10.1.1)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/36);  total (36/36)

GCGTAATTCTTCCTGATGGGTTAATTCCATAATAGACTCAATGAATTAGTGTAATAAATCGGTAAAAGGCTCAATGGTGACCGTTTCGCCCGCTGCAACATTACCGCGTTCCTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAACCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAGAACCAGGCGTTTTTTAATTTGCCGAAAGCAAAAGGTTTACCCGGTTTAATGGCGATTTTCCAGAAATTGATTTTGCCCACTTT  >  1/745842‑746318                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                  gCGTAATTCTTCCTGATGGGTTAATTCCATAATAGACTCAATGAATTAGTGTAATAAATCGGTAAAAGGCTCAATGGTGACCGTTTCGCCCGCTGCAACATTACCGCGTTCCTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGgcg                                                                                                                                                                                                                                    >  1:614060/1‑251 (MQ=255)                         ttCCATAATAGACTCAATGAATTAGTGTAATAAATCGGTAAAAGGCTCAATGGTGACCGTTTCGCCCGCTGCAACATTACCGCGTTCCTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGa                                                                                                                                                                                                            >  1:533445/1‑251 (MQ=255)                             ataataGACTCAATGAATTAGTGTAATAAATCGGTAAAAGGCTCAATGGTGACCGTTTCGCCCGCTGCAACATTACCGCGTTCCTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACgg                                                                                                                                                                                                         >  1:106572/1‑250 (MQ=255)                                    aCTCAATGAATTAGTGTAATAAATCGGTAAAAGGCTCAATGGTGACCGTTTCGCCCGCTGCAACATTACCGCGTTCCTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGa                                                                                                                                                                                                   <  1:896744/249‑1 (MQ=255)                                       cAATGAATTAGTGTAATAAATCGGTAAAAGGCTCAATGGTGACCGTTTCGCCCGCTGCAACATTACCGCGTTCCTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGa                                                                                                                                                                                                            >  1:586358/1‑237 (MQ=255)                                           gAATTAGTGTAATAAATCGGTAAAAGGCTCAATGGTGACCGTTTCGCCCGCTGCAACATTACCGCGTTCCTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCAcggcgg                                                                                                                                                                                                               <  1:646962/230‑1 (MQ=255)                                                 gtgtAATAAATCGGTAAAAGGCTCAATGGTGACCGTTTCGCCCGCTGCAACATTACCGCGTTCCTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAg                                                                                                                                                                                                 <  1:892846/238‑1 (MQ=255)                                                          aTCGGTAAAAGGCTCAATGGTGACCGTTTCGCCCGCTGCAACATTACCGCGTTCCTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGc                                                                                                                                                                           <  1:12677/251‑1 (MQ=255)                                                          aTCGGTAAAAGGCTCAATGGTGACCGTTTCGCCCGCTGCAACATTACCGCGTTCCTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGc                                                                                                                                                                           <  1:378156/251‑1 (MQ=255)                                                           tCGGTAAAAGGCTCAATGGTGACCGTTTCGCCCGCTGCAACATTACCGCGTTCCTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTcc                                                                                                                                                                                 <  1:448695/244‑1 (MQ=255)                                                              gTAAAAGGCTCAATGGTGACCGTTTCGCCCGCTGCAACATTACCGCGTTCCTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTa                                                                                                                                                                       >  1:600941/1‑251 (MQ=255)                                                                     gCTCAATGGTGACCGTTTCGCCCGCTGCAACATTACCGCGTTCCTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCg                                                                                                                                                                   >  1:755732/1‑248 (MQ=255)                                                                        cAATGGTGACCGTTTCGCCCGCTGCAACATTACCGCGTTCCTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGt                                                                                                                                                             <  1:783546/251‑1 (MQ=255)                                                                          aTGGTGACCGTTTCGCCCGCTGCAACATTACCGCGTTCCTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGttt                                                                                                                                                           >  1:719052/1‑251 (MQ=255)                                                                          aTGGTGACCGTTTCGCCCGCTGCAACATTACCGCGTTCCTGTTCCAGCACGATAAAACAATTACTCTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGa                                                                                                                                                                                              <  1:507971/216‑1 (MQ=255)                                                                                aCCGTTTCGCCCGCTGCAACATTACCGCGTTCCTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGa                                                                                                                                                      <  1:790345/250‑1 (MQ=255)                                                                                   gTTTCGCCCGCTGCAACATTACCGCGTTCCTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGcc                                                                                                                                                                    >  1:563367/1‑233 (MQ=255)                                                                                       cGCCCGCTGCAACATTACCGCGTTCCTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGt                                                                                                                                              <  1:888031/251‑1 (MQ=255)                                                                                             cTGCAACATTACCGCGTTCCTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCa                                                                                                                                          >  1:498175/1‑249 (MQ=255)                                                                                             cTGCAACATTACCGCGTTCCTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCAc                                                                                                                                         <  1:792735/250‑1 (MQ=255)                                                                                             cTGCAACATTACCGCGTTCCTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCAc                                                                                                                                         <  1:759090/250‑1 (MQ=255)                                                                                             cTGCAACATTACCGCGTTCCTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGGTGCa                                                                                                                                          >  1:501028/1‑249 (MQ=255)                                                                                                cAACATTACCGCGTTCCTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGa                                                                                                                                                                                                   <  1:395791/189‑1 (MQ=255)                                                                                                 aaCATTACCGCGTTCCTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAAtt                                                                                                                                    <  1:382727/251‑1 (MQ=255)                                                                                                 aaCATTACCGCGTTCCTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAAtt                                                                                                                                    <  1:251139/251‑1 (MQ=255)                                                                                                      tACCGCGTTCCTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATaa                                                                                                                               <  1:540627/251‑1 (MQ=255)                                                                                                             ttCCTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGtta                                                                                                                        >  1:276087/1‑249 (MQ=255)                                                                                                               ccTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCAcc                                                                                                                      <  1:489317/251‑1 (MQ=255)                                                                                                               ccTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCAcc                                                                                                                      <  1:800876/251‑1 (MQ=255)                                                                                                                cTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCa                                                                                                                     <  1:260987/251‑1 (MQ=255)                                                                                                                   ttCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAAt                                                                                                                                                                                            <  1:572670/177‑1 (MQ=255)                                                                                                                   ttCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGc                                                                                                                                                                     >  1:389822/1‑200 (MQ=255)                                                                                                                           cGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATaa                                                                                                                               <  1:797724/230‑1 (MQ=255)                                                                                                                            gATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCg                                                                                                         >  1:577395/1‑251 (MQ=255)                                                                                                                             aTAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGaa                                                                                                                                                                                             <  1:234646/166‑1 (MQ=255)                                                                                                                               aaaaCAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGt                                                                                                      <  1:389810/251‑1 (MQ=255)                                                                                                                                        aCTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAAtt                                                                                                                                    <  1:419092/212‑1 (MQ=255)                                                                                                                                                caAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAg                                                                                     >  1:146000/1‑251 (MQ=255)                                                                                                                                                     gAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGt                                                                                                      >  1:232548/1‑229 (MQ=255)                                                                                                                                                     gAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAGAAc                                                                                  <  1:674886/249‑1 (MQ=255)                                                                                                                                                     gAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAGAAc                                                                                  <  1:796698/249‑1 (MQ=255)                                                                                                                                                     gAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCCTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAGAAc                                                                                  <  1:662882/249‑1 (MQ=255)                                                                                                                                                        cTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAGAACCAGGc                                                                             <  1:223348/251‑1 (MQ=255)                                                                                                                                                          gAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATTGCGGGTTGCTCTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGccc                                                                                                 >  1:331653/1‑229 (MQ=255)                                                                                                                                                          gAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCg                                                                                                                                                 >  1:181033/1‑181 (MQ=255)                                                                                                                                                          gAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAGAACCAGGCGt                                                                           >  1:821705/1‑251 (MQ=255)                                                                                                                                                          gAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAGAACCAGGCGt                                                                           >  1:632878/1‑251 (MQ=255)                                                                                                                                                           aaTAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAGAACCAGGCGt                                                                           <  1:83329/250‑1 (MQ=255)                                                                                                                                                            aTAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAGAACCAGGCGttt                                                                         >  1:571805/1‑251 (MQ=255)                                                                                                                                                               aaTGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGGTGCCCGGCAAACCGCAGAACCAGGCGtttttt                                                                      >  1:537115/1‑251 (MQ=255)                                                                                                                                                                         ttGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAGAACCAGGCGTTTTTTAATTTGCCGa                                                            >  1:821237/1‑251 (MQ=255)                                                                                                                                                                                   cccGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAGAACCAGGCGTTTTTTAATTTGCCGAAAGCAAAAgg                                                  <  1:247585/250‑1 (MQ=255)                                                                                                                                                                                        ttGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAGAACCAGGCGTTTTTTAAt                                                                   >  1:752831/1‑229 (MQ=255)                                                                                                                                                                                           cACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCAACACCGGGTTGCCCGGCAAACCGCAGAACCAGGCGTTTTTTAATTTGCCGAAAGCAAAAGGTTTAccc                                           <  1:482320/250‑1 (MQ=255)                                                                                                                                                                                             ctttaaTTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCAcc                                                                                                                      <  1:423738/169‑1 (MQ=255)                                                                                                                                                                                             cTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAGAACCAGGCGTTTTTTAATTTGCCGAAAGCAAAAGGTTTACCCGGt                                        >  1:826355/1‑251 (MQ=255)                                                                                                                                                                                             cTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAGAACCAGGCGTTTTTTAATTTGCCGAAAGCAAAAGGTTTACCCGGt                                        >  1:394601/1‑251 (MQ=255)                                                                                                                                                                                               tCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAGAACCAGGCGTTTTTTAATTTGCCGAAAGCAAAAGGTTTACCCGGttt                                      <  1:57314/251‑1 (MQ=255)                                                                                                                                                                                                cAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAGAACCAGGCGTTTTTTAATTTGCCGAAAGCAAAAGGTTTACCCGGTTTa                                     >  1:751347/1‑251 (MQ=255)                                                                                                                                                                                                cAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAGAACCAGGCGTTTTTTAATTTGCCGAAAGCAAAAGGTTTACCCGGTTTa                                     >  1:319792/1‑251 (MQ=255)                                                                                                                                                                                                      gCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAGAACCAGGCGTTTTTTAATTTGCCGAAAGCAAAAGGTTTACCCGGTTTAATGGCg                               >  1:856690/1‑251 (MQ=255)                                                                                                                                                                                                         cTTGAGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAGAACCAGGCGTTTTTTAATTTGCCGAAAGCAAAAGGTTTACCCGGTTTAATGGCGAtt                            >  1:331081/1‑251 (MQ=255)                                                                                                                                                                                                             aGCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAGAACCAGGCGTTTTTTAATTTGCCGAAAGCAAAAGGTTTACCCGGTTTAATGGCGATTTTc                         <  1:94722/250‑1 (MQ=255)                                                                                                                                                                                                              gCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAGAAc                                                                                  >  1:308132/1‑192 (MQ=255)                                                                                                                                                                                                              gCGTTGACGTGATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAGAACCAGGCGTTTTTTAATTTGCCGAAAGCAAAAGGTTTACCCGGTTTAAt                                   <  1:457218/239‑1 (MQ=255)                                                                                                                                                                                                                        gATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAGAACCAGGCGTTTTTTAATTTGCCGAAAGCAAAAGGTTTACCCGGttt                                      >  1:516791/1‑226 (MQ=255)                                                                                                                                                                                                                        gATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAGAACCAGGCGTTTTTTAATTTGCCGAAAGCAAAAGGTTTACCCGGTTTAATGGCGATTTTCCAGAAATTGAtt             >  1:811522/1‑251 (MQ=255)                                                                                                                                                                                                                        gATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAGAACCAGGCGTTTTTTAATTTGCCGAAAGCAAAAGGTTTACCCGGTTTAATGGCGATTTTCCAGAAATTGAtt             >  1:194515/1‑251 (MQ=255)                                                                                                                                                                                                                        gATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAGAACCAGGCGTTTTTTAATTTGCCGAAAGCAAAAGGTTTACCCGGTTTAATGGCGATTTTCCAGAAATTGAtt             >  1:558801/1‑251 (MQ=255)                                                                                                                                                                                                                        gATAATACCCGCGTTGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAGAACCAGGCGTTTTTTAATTTGCCGAAAGCAAAAGGTTTACCCGGTTTAATGGCGATTTTCCAGAAATTGAtt             >  1:348219/1‑251 (MQ=255)                                                                                                                                                                                                                                     ttGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTcc                                                                                                                                                                                 <  1:154054/74‑1 (MQ=255)                                                                                                                                                                                                                                     ttGGAAATCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAGAACCAGGCGTTTTTTAATTTGCCGAAAGCAAAAGGTTTACCCGGTTTAATGGCGATTTTCCAGAAATTGATTTTGCCCACttt  >  1:612867/1‑249 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                  GCGTAATTCTTCCTGATGGGTTAATTCCATAATAGACTCAATGAATTAGTGTAATAAATCGGTAAAAGGCTCAATGGTGACCGTTTCGCCCGCTGCAACATTACCGCGTTCCTGTTCCAGCACGATAAAACAATTACTTTTCACAAAGGAGCTGAATAAATGGGAGCCTTGGAAACCCACCGGTTGCACTTCAATTTGCCCTTGAGCGTTGACGTGATAATACCCGCGTTGGAAACCTAATCGTCCCGGCGCTTTTTTCAAATTCACGGCGGCGACGGCAGGGAAGCGAATCGGCGCTTTCCATTGGCTGTAGCCGCTGAGTTTGGCGATGGCGGGTTGCACTAATTGATAAAATGTCACCAACGCCGACACCGGGTTGCCCGGCAAACCGCAGAACCAGGCGTTTTTTAATTTGCCGAAAGCAAAAGGTTTACCCGGTTTAATGGCGATTTTCCAGAAATTGATTTTGCCCACTTT  >  1/745842‑746318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑