Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	723,071	A→G	I97V (ATT→GTT)	unknown →	4‑hydroxy‑tetrahydrodipicolinate reductase (EC 1.17.1.8)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	723,071	0	A	G	100.0%	213.4 / NA	77	I97V (ATT→GTT)	unknown	4‑hydroxy‑tetrahydrodipicolinate reductase (EC 1.17.1.8)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/36);  total (41/36)

GGCGGTACATAACGCGGAAGGCGTGGAATTAGGTGCGGCGTTTGAGCGCAAAGGTTCCTCATTGGTGGGCGCCGATGCCGGCGAATTGGCGGGAATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCATTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCAGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACC  >  1/722842‑723316                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      ggCGGTACATAACGCGGAAGGCGTGGAATTAGGTGCGGCGTTTGAGCGCAAAGGTTCCTCATTGGTGGGCGCCGATGCCGGCGAATTGGCGGGGATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTtg                                                                                                                                                                                                                                  >  1:691796/1‑251 (MQ=255)  gCGGTACATAACGCGGAAGGCGTGGAATTAGGTGCGGCGTTTGAGCGCAAAGGTTCCTCATTGGTGGGCGCCGATGCCGGCGAATTGGCGGGGATCGGCAACTTAGGCATTAAGGGCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTtga                                                                                                                                                                                                                                 >  1:496309/1‑251 (MQ=255)     gTACATAACGCGGAAGGCGTGGAATTAGGTGCGGCGTTTGAGCGCAAAGGTTCCTCATTGGTGGGCGCCGATGCCGGCGAATTGGCGGGGATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTtgatga                                                                                                                                                                                                                              >  1:178867/1‑251 (MQ=255)     gTACATAACGCGGAAGGCGTGGAATTAGGTGCGGCGTTTGAGCGCAAAGGTTCCTCATTGGTGGGCGCCGATGCCGGCGAATTGGCGGGGATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTtgatga                                                                                                                                                                                                                              >  1:177514/1‑251 (MQ=255)     gTACATAACGCGGAAGGCGTGGAATTAGGTGCGGCGTTTGAGCGCAAAGGTTCCTCATTGGTGGGCGCCGATGCCGGCGAATTGGCGGGGATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCACCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTtgatga                                                                                                                                                                                                                              >  1:432415/1‑251 (MQ=255)           aaCGCGGAAGGCGTGGAATTAGGTGCGGCGTTTGAGCGCAAAGGTTCCTCATTGGTGGGCGCCGATGCCGGCGAATTGGCGGGGATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCgg                                                                                                                                                                                                                        >  1:591739/1‑251 (MQ=255)             cgcgGAAGGCGTGGAATTAGGTGCGGCGTTTGAGCGCAAAGGTTCCTCATTGGTGGGCGCCGATGCCGGCGAATTGGCGGGGATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCa                                                                                                                                                                                                                      >  1:92564/1‑251 (MQ=255)                    ggCGTGGAATTAGGTGCGGCGTTTGAGCGCAAAGGTTCCTCATTGGTGGGCGCCGATGCCGGCGAATTGGCGGGGATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCgg                                                                                                                                                                                                                >  1:896599/1‑250 (MQ=255)                        tGGAATTAGGTGCGGCGTTTGAGCGCAAAGGTTCCTCATTGGTGGGCGCCGATGCCGGCGAATTGGCGGGGATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAAtt                                                                                                                                                                                                           >  1:872901/1‑251 (MQ=255)                          gAATTAGGTGCGGCGTTTGAGCGCAAAGGTTCCTCATTGGTGGGCGCCGATGCCGGCGAATTGGCGGGGATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTaa                                                                                                                                                                                                         >  1:118957/1‑251 (MQ=255)                          gAATTAGGTGCGGCGTTTGAGCGCAAAGGTTCCTCATTGGTGGGCGCCGATGCCGGCGAATTGGCGGGGATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTaa                                                                                                                                                                                                         >  1:49808/1‑251 (MQ=255)                            aTTAGGTGCGGCGTTTGAGCGCAAAGGTTCCTCATTGGTGGGCGCCGATGCCGGCGAATTGGCGGGGATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAAt                                                                                                                                                                                                       <  1:716172/251‑1 (MQ=255)                             ttAGGTGCGGCGTTTGAGCGCAAAGGTTCCTCATTGGTGGGCGCCGATGCCGGCGAATTGGCGGGGATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATc                                                                                                                                                                                                      <  1:16679/251‑1 (MQ=255)                                      gcgTTTGAGCGCAAAGGTTCCTCATTGGTGGGCGCCGATGCCGGCGAATTGGCGGGGATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACggg                                                                                                                                                                                                                                       <  1:378035/209‑1 (MQ=255)                                          ttGAGCGCAAAGGTTCCTCATTGGTGGGCGCCGATGCCGGCGAATTGGCGGGGATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAg                                                                                                                                                                                         <  1:596478/251‑1 (MQ=255)                                              gcgcAAAGGTTCCTCATTGGTGGGCGCCGATGCCGGCGAATTGGCGGGGATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGAGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCCTTTTGTGTTGCCAACCATAAAAAAATCGTTATCCGCACCACGGGTTTTGATGACGCCCGCAAAGCGGCAATTAAATctgcctcttatacacatt                                                                                                                                                                                     >  1:860297/1‑234 (MQ=255)                                                 cAAAGGTTCCTCATTGGTGGGCGCCGATGCCGGCGAATTGGCGGGGATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGAGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTa                                                                                                                                                                                  >  1:261109/1‑251 (MQ=255)                                                     ggTTCCTCATTGGTGGGCGCCGATGCCGGCGAATTGGCGGGGATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCgg                                                                                                                                                                              >  1:837788/1‑250 (MQ=255)                                                     ggTTCCTCATTGGTGGGCGCCGATGCCGGCGAATTGGCGGGGATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAAAGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCgg                                                                                                                                                                              >  1:519382/1‑250 (MQ=255)                                                       ttCCTCATTGGTGGGCGCCGATGCCGGCGAATTGGCAGGGATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGCGAAAGATCGTTTCGATTTGCTGATTGATTTCACCCGCCCGGGAAGGCACATTGGAACACTTGGCATTTTGTGTCACCAATCGTAAAAAAATCGTTATCGGCACCACGGGTTTTGATGATGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCgtg                                                                                                                                                                             <  1:66080/251‑1 (MQ=255)                                                         ccTCATTGGTGGGCGCCGATGCCGGCGAATTGGCGGGGATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCaa                                                                                                                                                                                                                     <  1:597447/208‑1 (MQ=255)                                                         ccTCATTGGTGGGCGCCGATGCCGGCGAATTGGCGGGGATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCgtgt                                                                                                                                                                            >  1:212110/1‑249 (MQ=255)                                                         ccTCATTGGTGGGCGCCGATGCCGGCGAATTGGCGGGGATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCgtg                                                                                                                                                                             >  1:582097/1‑248 (MQ=255)                                                         ccTCATTGGTGGGCGCCGATGCCGGCGAATTGGCGGGGATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGttt                                                                                                                                                                          <  1:568871/251‑1 (MQ=255)                                                         ccTCATTGGTGGGCGCCGATGCCGGCGAATTGGCGGGGATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGttt                                                                                                                                                                          <  1:689798/251‑1 (MQ=255)                                                           tCATTGGTGGGCGCCGATGCCGGCGAATTGGCGGGGATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATCTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTg                                                                                                                                                                         >  1:610461/1‑250 (MQ=255)                                                            cATTGGTGGGCGCCGATGCCGGCGAATTGGCGGGGATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCt                                                                                                                                                                       <  1:152581/251‑1 (MQ=255)                                                                                        ggCGGGGATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTgg                                                                                                                                           >  1:383781/1‑251 (MQ=255)                                                                                            gggaTCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGACAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCtt                                                                                                                                       >  1:443927/4‑251 (MQ=255)                                                                                              gaTCGGCAACTTAGGCCTTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGCGTCGGCGTGAATTTGGTCTTTa                                                                                                                                     >  1:282920/2‑251 (MQ=255)                                                                                                 cGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAat                                                                                                                                  >  1:577589/1‑251 (MQ=255)                                                                                                            gCATTAAGGTCGGCGATGACTTAAACGCGGTGAAGGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAg                                                                                                                                                                                         <  1:631766/185‑1 (MQ=255)                                                                                                            gCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGaaaaa                                                                                                                       >  1:105239/1‑251 (MQ=255)                                                                                                                      cGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAgg                                                                                                             <  1:36933/251‑1 (MQ=255)                                                                                                                      cGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAgg                                                                                                             <  1:366235/251‑1 (MQ=255)                                                                                                                              aCTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATggg                                                                                                      >  1:285323/1‑250 (MQ=255)                                                                                                                              aCTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGc                                                                                                     <  1:768174/251‑1 (MQ=255)                                                                                                                               cTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCg                                                                                                    >  1:179860/1‑251 (MQ=255)                                                                                                                                 tAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGat                                                                                                  <  1:778852/251‑1 (MQ=255)                                                                                                                                         gTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTtga                                                                                                                                                                                                                                 >  1:477486/1‑116 (MQ=255)                                                                                                                                         gTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCa                                                                                                                <  1:147158/229‑1 (MQ=255)                                                                                                                                         gTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGatta                                                                                                >  1:399135/1‑245 (MQ=255)                                                                                                                                         gTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGa                                                                                          >  1:104952/1‑251 (MQ=255)                                                                                                                                         gTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGa                                                                                          >  1:158407/1‑251 (MQ=255)                                                                                                                                         gTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGa                                                                                          >  1:602439/1‑251 (MQ=255)                                                                                                                                         gTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGa                                                                                          >  1:600100/1‑251 (MQ=255)                                                                                                                                          tGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGAc                                                                                         <  1:539429/251‑1 (MQ=255)                                                                                                                                           gAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACa                                                                                        >  1:479651/1‑251 (MQ=255)                                                                                                                                                   gTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAat                                                                                 >  1:727523/1‑250 (MQ=255)                                                                                                                                                        gATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAatt                                                                                                                                 >  1:22859/1‑197 (MQ=255)                                                                                                                                                                gattgattTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGa                                                                                                          <  1:11784/212‑1 (MQ=255)                                                                                                                                                                  ttgattTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGTAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGcaccac                                                                 <  1:83183/251‑1 (MQ=255)                                                                                                                                                                    gattTCACCCGCCC‑GGAAGGCACATTGGAACATTTGTCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAAGTGAATCGGCAGCCGAAAAGACCGCTGTCGTGTTTGCTTCAAATTGCAGTGTCGGCGTGAATTTGGTCTGTAAATTAGTGGAAAAAGCCGGCAAgg                                                                                                             <  1:872972/205‑1 (MQ=255)                                                                                                                                                                    gattTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGaaaa                                                                                                                        >  1:401606/1‑194 (MQ=255)                                                                                                                                                                    gattTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAACTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCg                                                               >  1:564329/1‑251 (MQ=255)                                                                                                                                                                     attTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGc                                                              <  1:564040/251‑1 (MQ=255)                                                                                                                                                                          aCCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATaa                                                         <  1:806613/251‑1 (MQ=255)                                                                                                                                                                            ccgccc‑gGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAg                                                       <  1:212593/251‑1 (MQ=255)                                                                                                                                                                             cgccc‑gGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGt                                                      <  1:248903/251‑1 (MQ=255)                                                                                                                                                                                           caTTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTc                                          >  1:209740/1‑250 (MQ=255)                                                                                                                                                                                             ttGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCgtg                                                                                                                                                                             <  1:622377/117‑1 (MQ=255)                                                                                                                                                                                             ttGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGaa                                                                                                                          <  1:389628/168‑1 (MQ=255)                                                                                                                                                                                                 aaCATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTAc                                    <  1:640287/250‑1 (MQ=255)                                                                                                                                                                                                 aaCATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTAc                                    <  1:449103/250‑1 (MQ=255)                                                                                                                                                                                                     tttGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCgcgc                               <  1:117717/251‑1 (MQ=255)                                                                                                                                                                                                         gCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGcaccac                                                                 <  1:283933/213‑1 (MQ=255)                                                                                                                                                                                                          cATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATgcgc                                              >  1:132080/1‑231 (MQ=255)                                                                                                                                                                                                               tgtgTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATggg                     <  1:470221/251‑1 (MQ=255)                                                                                                                                                                                                               tgtgTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATggg                     <  1:499402/251‑1 (MQ=255)                                                                                                                                                                                                               tgtgTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATggg                     <  1:316873/251‑1 (MQ=255)                                                                                                                                                                                                                gtgtTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATggg                     >  1:202219/1‑250 (MQ=255)                                                                                                                                                                                                                        aaCCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCaa                                                                                                                                                                                                                     <  1:884101/50‑1 (MQ=255)                                                                                                                                                                                                                          ccATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGt                                                      <  1:250437/207‑1 (MQ=255)                                                                                                                                                                                                                          ccATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTAc                                    <  1:886353/225‑1 (MQ=255)                                                                                                                                                                                                                          ccATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTg          >  1:482563/1‑251 (MQ=255)                                                                                                                                                                                                                             tAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTTTAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCa       >  1:317497/1‑251 (MQ=255)                                                                                                                                                                                                                                  aaaTCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAAcc  <  1:86861/251‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      GGCGGTACATAACGCGGAAGGCGTGGAATTAGGTGCGGCGTTTGAGCGCAAAGGTTCCTCATTGGTGGGCGCCGATGCCGGCGAATTGGCGGGAATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCC‑GGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCATTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCAGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACC  >  1/722842‑723316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑