Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 721,616 G→T E324D (GAG→GAT)  unknown → Thiamine‑monophosphate kinase (EC 2.7.4.16)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1721,6160GT100.0% 171.9 / NA 64E324D (GAG→GAT) unknownThiamine‑monophosphate kinase (EC 2.7.4.16)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (35/29);  total (35/29)

GAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGAGCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCACTTGGGGCAGTTTGGTCGGCGTGATTTTAGGCGCGTTATTGCTGCCGGTACTCGGCGCAAAAACATTTTTTATTTTGACCGCACTTTGCTTCTTCATCGGCATTTGGCTATGCGAACG  >  1/721380‑721858
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                  
gAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGt                                                                                                                                                                                                                                      >  1:89542/1‑251 (MQ=255)
gAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGt                                                                                                                                                                                                                                      >  1:345655/1‑251 (MQ=255)
             aaGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGTGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACg                                                                                                                                                                                                                         <  1:835463/251‑1 (MQ=255)
             aaGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACg                                                                                                                                                                                                                         <  1:246833/251‑1 (MQ=255)
                      tGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTacaa                                                                                                                                                                                                                <  1:405256/251‑1 (MQ=255)
                        gCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTacaac                                                                                                                                                                                                               <  1:86550/250‑1 (MQ=255)
                        gCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTacaac                                                                                                                                                                                                               <  1:731605/250‑1 (MQ=255)
                            aaGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACg                                                                                                                                                                                                                         >  1:333875/1‑236 (MQ=255)
                                    cAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACg                                                                                                                                                                                                  >  1:206319/1‑251 (MQ=255)
                                               gTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTacaac                                                                                                                                                                                                               <  1:559096/227‑1 (MQ=255)
                                                            gACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATcc                                                                                                                                                                                <  1:739772/245‑1 (MQ=255)
                                                             aCTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTc                                                                                                                                                                           <  1:556538/249‑1 (MQ=255)
                                                             aCTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCAt                                                                                                                                                                         <  1:494236/251‑1 (MQ=255)
                                                             aCTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCAt                                                                                                                                                                         <  1:350670/251‑1 (MQ=255)
                                                                     cTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTcg                                                                                                                                                                  >  1:476720/1‑250 (MQ=255)
                                                                     cTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTcg                                                                                                                                                                  >  1:333770/1‑250 (MQ=255)
                                                                       ttGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTcgcg                                                                                                                                                                >  1:747452/1‑250 (MQ=255)
                                                                         gTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAAt                                                                                                                                                                                                                                          >  1:643122/1‑174 (MQ=255)
                                                                         gTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTac                                                                                                                                                                                                                  >  1:465638/1‑198 (MQ=255)
                                                                               ccGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTacaa                                                                                                                                                                                                                >  1:128355/1‑194 (MQ=255)
                                                                                         caacaaTATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGtt                                                                                                                                             >  1:664874/1‑251 (MQ=255)
                                                                                         caacaaTATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGCTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGTGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCCCCGGtt                                                                                                                                             >  1:223533/1‑251 (MQ=255)
                                                                                              atatCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTg                                                                                                                                        <  1:836877/251‑1 (MQ=255)
                                                                                                               aaaGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCAc                                                                                                                       <  1:586866/251‑1 (MQ=255)
                                                                                                               aaaGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCAc                                                                                                                       <  1:760815/251‑1 (MQ=255)
                                                                                                                  gCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGCACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCACTTg                                                                                                                    >  1:445238/1‑251 (MQ=255)
                                                                                                                    ccTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCACTTggg                                                                                                                  <  1:741851/251‑1 (MQ=255)
                                                                                                                          gcATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTgggg                                                                                                                                                          <  1:191225/205‑1 (MQ=255)
                                                                                                                            aTCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCACTTGGGGCAGTTTg                                                                                                          >  1:468778/1‑251 (MQ=255)
                                                                                                                                    gTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACt                                                                                                                                                                                                                                <  1:123893/125‑1 (MQ=255)
                                                                                                                                      gCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCACTTGGGGCAGTTTTGTCGGCGTGa                                                                                                >  1:660273/1‑251 (MQ=255)
                                                                                                                                       ccATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGGGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACg                                                                                                                                                                                                                         >  1:790787/1‑129 (MQ=255)
                                                                                                                                       ccATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGCGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGTACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCACTTGGGGCAGTTTGGTCGGCGTGAt                                                                                               >  1:259194/1‑251 (MQ=255)
                                                                                                                                       ccATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTg                                                                                                                                                                                              >  1:329595/1‑156 (MQ=255)
                                                                                                                                       ccATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGtt                                                                                                                                             <  1:55056/205‑1 (MQ=255)
                                                                                                                                       ccATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCACTTGGGGCAGTTTGGt                                                                                                        <  1:487633/242‑1 (MQ=255)
                                                                                                                                       ccATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCACTTGGGGCAGTTTGGTCGGCGTGAt                                                                                               >  1:25736/1‑251 (MQ=255)
                                                                                                                                       ccATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCACTTGGGGCAGTTTGGTCGGCGTGAt                                                                                               >  1:319052/1‑251 (MQ=255)
                                                                                                                                       ccATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCACTTGGGGCAGTTTGGTCGGCGTGAt                                                                                               >  1:375388/1‑251 (MQ=255)
                                                                                                                                       ccATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCACTTGGGGCAGTTTGGTCGGCGTGAt                                                                                               >  1:900055/1‑251 (MQ=255)
                                                                                                                                            taCCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAAc                                                                                                                                                                                                   >  1:311937/1‑146 (MQ=255)
                                                                                                                                            taCCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTgcgc                                                                                                                                                                                     <  1:226951/160‑1 (MQ=255)
                                                                                                                                            taCCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTc                                                                                                                                                                           >  1:166991/1‑170 (MQ=255)
                                                                                                                                                 gTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCACTTGGGGCAGTTTGGTCGGCGTGATTTTAGGcgcg                                                                                     >  1:211288/1‑251 (MQ=255)
                                                                                                                                                              tCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCACTTGGGGCAGTTTGGTCGGCGTGATTTTAGGCGCGTTATTGCTGCCgg                                                                        <  1:673551/251‑1 (MQ=255)
                                                                                                                                                                   aCGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCACTTGGGGCAGTTTGGTCGGCGTGATTTTAGGCGCGTTATTGCTGCCGGTACTc                                                                   <  1:461183/251‑1 (MQ=255)
                                                                                                                                                                   aCGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCACTTGGGGCAGTTTGGTCGGCGTGATTTTAGGCGCGTTATTGCTGCCGGTACTc                                                                   <  1:39191/251‑1 (MQ=255)
                                                                                                                                                                        ggCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCACTTGGGGCAGTTTGGTCGGCGTGATTTTAG‑‑GCGTTATTGCTGCCGGTACTCGGCGCaa                                                            >  1:346868/1‑251 (MQ=255)
                                                                                                                                                                                aCGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGCGAATTTGCGCAATCCTATTCAt                                                                                                                                                                         >  1:492905/1‑136 (MQ=255)
                                                                                                                                                                                    aTTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCACTTGGGGCAGTTTGGTCGGCGTGATTTTAGGCGCGTTATTGCTGCCGGTACTCGGCGCAAAAACAttttt                                                  >  1:475946/1‑251 (MQ=255)
                                                                                                                                                                                         gTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCACTTGGGGCAGTTTGGTCGGCGTGATTTTAGGCGCGTTATTGCTGCCGGTACTCGGCGCAAAAACATTTTTTAttt                                             >  1:19689/1‑251 (MQ=255)
                                                                                                                                                                                         gTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCACTTGGGGCAGTTTGGTCGGCGTGATTTTAGGCGCGTTATTGCTGCCGGTACTCGGCGCAAAAACATTTTTTAttt                                             >  1:560516/1‑251 (MQ=255)
                                                                                                                                                                                                       ggCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCg                                                                                                                                                                                                      <  1:303713/84‑1 (MQ=255)
                                                                                                                                                                                                       ggCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATcc                                                                                                                                                                                <  1:838626/106‑1 (MQ=255)
                                                                                                                                                                                                         cTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCACTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCACTTGGGGCAGTTTGGTCGGCGTGATTTTAGGCGCGTTATTGCTGCCGGTACTCGGCGCAAAAACATTTTTTATTTTGACCGCACTTTGct                              <  1:189169/250‑1 (MQ=255)
                                                                                                                                                                                                                   aGCTTGAGTTGCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCACTTGGGGCAGTTTGGTCGGCGTGATTTTAGGCGCGTTATTGCTGCCGGTACTCGGCGCAAAAACATTTTTTATTTTGACCGCACTTTGCTTCTTCATCgg                    >  1:840171/1‑250 (MQ=255)
                                                                                                                                                                                                                             gCCATACGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCACTTGGGGCAGTTTGGTCGGCGTGATTTTAGGCGCGTTATTGCTGCCGGTACTCGGCGCAAAAACATTTTTTATTTTGACCGCACTTTGCTTCTTCATCGGCATTTGGCTAt         >  1:604847/1‑251 (MQ=255)
                                                                                                                                                                                                                                   cGGTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGc                                                                                                                         <  1:172996/133‑1 (MQ=255)
                                                                                                                                                                                                                                     gTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCACTTGGGGCAGTTTGGTCGGCGTGATTTTAGGCGCGTTATTGCTGCCGGTACt                                                                    <  1:671847/184‑1 (MQ=255)
                                                                                                                                                                                                                                     gTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCACTTGGGGCAGTTTGGTCGGCGTGATTTTAGGCGCGTTATTGCTGCCGGTACTCGGCGCAAAAACATTTTTTATTTTGACCGCACTTTGCTTCTTCATCGGCATTTGGc            <  1:661066/240‑1 (MQ=255)
                                                                                                                                                                                                                                     gTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCACTTGGGGCAGTTTGGTCGGCGTGATTTTAGGCGCGTTATTGCTGCCGGTACTCGGCGCAAAAACATTTTTTATTTTGACCGCACTTTGCTTCTTCATCGGCATTTGGc            <  1:644747/240‑1 (MQ=255)
                                                                                                                                                                                                                                     gTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCACTTGGGGCAGTTTGGTCGGCGTGATTTTAGGCGCGTTATTGCTGCCGGTACTCGGCGCAAAAACATTTTTTATTTTGACCGCACTTTGCTTCTTCATCGGCATTTGGCTATGCGAACg  >  1:499751/1‑250 (MQ=255)
                                                                                                                                                                                                                                     gTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCACTTGGGGCAGTTTGGTCGGCGTGATTTTAGGCGCGTTATTGCTGCCGGTACTCGGCGCAAAAACATTTTTTATTTTGACCGCACTTTGCTTCTTCATCGGCATTTGGCTATGCGAACg  >  1:468030/1‑250 (MQ=255)
                                                                                                                                                                                                                                     gTTTTGATCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAA‑TTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCACTTGGGGCAGTTTGGTCGGCGTGATTTTAGGCGCGTTATTGCTGCCGGTACTCGGCGCAAAAACATTTTTTATTTTGACCGCACTTTGCTTCTTCATCGGCATTTGGCTATGCGa     >  1:696503/1‑246 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                  
GAGTTGGTCGCCGAAGTCGGTTTGGCGAAAGCGGAACAGTTTGCCTTGTCCGGCGGCGAAGACTACGAACTTTGTTTCACCGTACCTGACAACAATATCGTCAAATTAGAAAAAGCCCTGGCGCATCTCGGCGTGCCATATACCTGTATCGGGCAAATTCGTCACGCGGGCAAAAAACGTATTCAGTTTCAACGCAACGGGCTGGCGGTCGAGCTTGAGTTGCCATACGGTTTTGAGCATTTCAAATAGGTTTCACTTATGACGTCATTACAACATCCGCTCGAACGGGTGAATTTGCGCAATCCTATTCATTTTTTCGCGCTGGGGTTCGGCTCCGGTTTATTGCGTCCCGCGCCCGGCACTTGGGGCAGTTTGGTCGGCGTGATTTTAGGCGCGTTATTGCTGCCGGTACTCGGCGCAAAAACATTTTTTATTTTGACCGCACTTTGCTTCTTCATCGGCATTTGGCTATGCGAACG  >  1/721380‑721858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑