Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 714,493 T→C T105T (ACT→ACC)  unknown → 2‑keto‑3‑deoxy‑D‑arabino‑heptulosonate‑7‑ phosphate synthase I alpha (EC 2.5.1.54)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1714,4930TC100.0% 225.9 / NA 81T105T (ACT→ACC) unknown2‑keto‑3‑deoxy‑D‑arabino‑heptulosonate‑7‑ phosphate synthase I alpha (EC 2.5.1.54)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/40);  total (41/40)

CCCATCGCGTTATTAGAAAAATTTCCTGCCAGCGAGGTTGCGGTAAAAACCGTAGAACAAGCCCGCGAAGAAGCACACGACATTATTCATGGCAAAGATGATCGCCTGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACTACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTATTTAGCGGATTTCATGAGCTGGGGTGCCATCGGTGCAAGAACCACCGAATCCCAAGTACACCGTGAACTGGCTTCCGGTTTATCCTGCGCG  >  1/714248‑714739
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      
cccATCGCGTTATTAGAAAAATTTCCTGCCAGCGAGGTTGCGGTAAAAACCGTAGAACAAGCCCGCGAAGAAGCACACGACATTATTCATGGCAAAGATGATCGCCTGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGt                                                                                                                                                                                                                                                   >  1:623636/1‑251 (MQ=255)
    tCGCGTTATTAGAAAAATTTCCTGCCAGCGAGGTTGCGGTAAAAACCGTAGAACAAGCCCGCGAAGAAGCACACGACATTATTCATGGCAAAGATGATCGCCTGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGt                                                                                                                                                                                                                                               <  1:120632/251‑1 (MQ=255)
    tCGCGTTATTAGAAAAATTTCCTGCCAGCGAGGTTGCGGTAAAAACCGTAGAACAAGCCCGCGAAGAAGCACACGACATTATTCATGGCAAAGATGATCGCCTGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGt                                                                                                                                                                                                                                               <  1:24072/251‑1 (MQ=255)
          tattaGAAAAATTTCCTGCCAGCGAGGTTGCGGTAAAAACCGTAGAACAAGCCCGCGAAGAAGCACACGACATTATTCATGGCAAAGATGATCGCCTGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGaaa                                                                                                                                                                                                                                         <  1:27365/251‑1 (MQ=255)
                  aaaTTTCCTGCCAGCGAGGTTGCGGTAAAAACCGTAGAACAAGCCCGCGAAGAAGCACACGACATTATTCATGGCAAAGATGATCGCCTGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCttaat                                                                                                                                                                                                                                 <  1:568240/251‑1 (MQ=255)
                   aaTTTCCTGCCAGCGAGGTTGCGGTAAAAACCGTAGAACAAGCCCGCGAAGAAGCACACGACATTATTCATGGCAAAGATGATCGCCTGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCttaatt                                                                                                                                                                                                                                <  1:248190/251‑1 (MQ=255)
                     tttCCTGCCAGCGAGGTTGCGGTAAAAACCGTAGAACAAGCCCGCGAAGAAGCACACGACATTATTCATGGCAAAGATGATCGCCTGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCttaattaa                                                                                                                                                                                                                              >  1:541963/1‑251 (MQ=255)
                          tGCCAGCGAGGTTGCGGTAAAAACCGTAGAACAAGCCCGCGAAGAAGCACACGACATTATTCATGGCAAAGATGATCGCCTGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGAcc                                                                                                                                                                                                                         <  1:901942/251‑1 (MQ=255)
                          tGCCAGCGAGGTTGCGGTAAAAACCGTAGAACAAGCCCGCGAAGAAGCACACGACATTATTCATGGCAAAGATGATCGCCTGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGAcc                                                                                                                                                                                                                         <  1:323955/251‑1 (MQ=255)
                                                 ccGTAGAACAAGCCCGCGAAGAAGCACACGACATTATTCATGGCAAAGATGATCGCCTGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAaat                                                                                                                                                                                                              >  1:642363/1‑239 (MQ=255)
                                                 ccGTAGAACAAGCCCGCGAAGAAGCACACGACATTATTCATGGCAAAGATGATCGCCTGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGcc                                                                                                                                                                                                  <  1:790269/251‑1 (MQ=255)
                                                  cGTAGAACAAGCCCGCGAAGAAGCACACGACATTATTCATGGCAAAGATGATCGCCTGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCt                                                                                                                                                                                                 <  1:624384/251‑1 (MQ=255)
                                                      gAACAAGCCCGCGAAGAAGCACACGACATTATTCATGGCAAAGATGATCGCCTGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGc                                                                                                                                                                                                   <  1:89905/245‑1 (MQ=255)
                                                      gAACAAGCCCGCGAAGAAGCACACGACATTATTCATGGCAAAGATGATCGCCTGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGaa                                                                                                                                                                                             >  1:491004/1‑251 (MQ=255)
                                                                gcgAAGAAGCACACGACATTATTCATGGCAAAGATGATCGCCTGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCttaatta                                                                                                                                                                                                                               <  1:549872/207‑1 (MQ=255)
                                                                     gaagCACACGACATTATTCATGGCAAAGATGATCGCCTGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTAt                                                                                                                                                                              >  1:747054/1‑251 (MQ=255)
                                                                        gCACACGACATTATTCATGGCAAAGATGATCGCCTGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAGGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATgg                                                                                                                                                                            >  1:399384/1‑250 (MQ=255)
                                                                               aCATTATTCATGGCAAAGATGATCGCCTGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTg                                                                                                                                                                                                                                             <  1:235605/178‑1 (MQ=255)
                                                                               aCATTATTCATGGCAAAGATGATCGCCTGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTaaa                                                                                                                                                                    <  1:833695/251‑1 (MQ=255)
                                                                               aCATTATTCATGGCAAAGATGATCGCCTGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTaaa                                                                                                                                                                    >  1:489710/1‑251 (MQ=255)
                                                                               aCATTATTCATGGCAAAGATGATCGCCTGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTaaa                                                                                                                                                                    >  1:545217/1‑251 (MQ=255)
                                                                               aCATTATTCATGGCAAAGATGATCGCCTGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCGTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTaaa                                                                                                                                                                    >  1:608231/1‑251 (MQ=255)
                                                                                cATTATTCATGGCAAAGATGATCGCCTGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAat                                                                                                                                                                   >  1:719708/1‑251 (MQ=255)
                                                                                    attCATGGCAAAGATGATCGCCTGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAattatt                                                                                                                                                               >  1:109730/1‑251 (MQ=255)
                                                                                        aTGGCAAAGATGATCGCCTGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATcc                                                                                                                                                           <  1:807727/251‑1 (MQ=255)
                                                                                           gCAAAGATGATCGCCTGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGa                                                                                                                                                                                                                           >  1:898944/1‑184 (MQ=255)
                                                                                                  tgatCGCCTGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACg                                                                                                                                                 <  1:834025/251‑1 (MQ=255)
                                                                                                          tGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAAc                                                                                                                                          >  1:896274/1‑250 (MQ=255)
                                                                                                            ttGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGt                                                                                                                                                                                <  1:723804/210‑1 (MQ=255)
                                                                                                              ggtggtGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATAc                                                                                                                                                                                                         >  1:225211/1‑183 (MQ=255)
                                                                                                                                               gAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGat                                                                                                              >  1:519686/1‑241 (MQ=255)
                                                                                                                                                        aTTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGAc                                                                                                                                                                                                                          >  1:64336/1‑124 (MQ=255)
                                                                                                                                                           ggAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACt                                                                                                                                                                                                        >  1:492006/1‑139 (MQ=255)
                                                                                                                                                           ggAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCacac                                                                                                    >  1:259251/1‑239 (MQ=255)
                                                                                                                                                            gAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATAc                                                                                                                                                                                                         <  1:784835/137‑1 (MQ=255)
                                                                                                                                                            gAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTATTTAg                                                                                        >  1:36735/1‑250 (MQ=255)
                                                                                                                                                            gAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTATTTAg                                                                                        >  1:488953/1‑250 (MQ=255)
                                                                                                                                                             aaTATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTATTTAGCg                                                                                      >  1:708755/1‑251 (MQ=255)
                                                                                                                                                                atGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTATTTAGCGGAt                                                                                   >  1:254023/1‑251 (MQ=255)
                                                                                                                                                                      aaCGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCATCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTATTTAGCGGATTTCATg                                                                             <  1:441877/251‑1 (MQ=255)
                                                                                                                                                                         gCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGAcc                                                                                                                                                                                                                         <  1:28980/108‑1 (MQ=255)
                                                                                                                                                                         gCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAac                                                                                                                                                                                            <  1:680986/137‑1 (MQ=255)
                                                                                                                                                                         gCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGCTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTATTTAGCGGATTTCATGAg                                                                           >  1:454491/1‑250 (MQ=255)
                                                                                                                                                                          cATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACg                                                                                                                                                 <  1:709758/179‑1 (MQ=255)
                                                                                                                                                                          cATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTATTTAGCGGATTTCATGAGc                                                                          <  1:887737/250‑1 (MQ=255)
                                                                                                                                                                           attaaattaaTGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGt                                                                                                                                                                                >  1:22817/1‑147 (MQ=255)
                                                                                                                                                                              aaattaaTGCGTGAAAAATTCAAAGATACCTTACAAATCGTCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTATTTAGCGGATTTCATGAGCTgggg                                                                     >  1:45450/1‑251 (MQ=255)
                                                                                                                                                                              aaattaaTGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATcac                                                                                                      >  1:899300/1‑218 (MQ=255)
                                                                                                                                                                              aaattaaTGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTATTTAGCGGATTTCATGAGCTgggg                                                                     >  1:69522/1‑251 (MQ=255)
                                                                                                                                                                                attaaTGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTATTTAGCGGATTTCATGAGCTGGGGTg                                                                   >  1:358284/1‑251 (MQ=255)
                                                                                                                                                                                 ttaaTGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTATTTAGCGGATTTCATGAGCTGGGGTGc                                                                  <  1:533685/251‑1 (MQ=255)
                                                                                                                                                                                   aaTGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTATTTAGCGGATTTCATGAGCTGGGGTGCCa                                                                <  1:844865/251‑1 (MQ=255)
                                                                                                                                                                                         tGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTATTTAGCGGATTTCATGAGCTGGGGTGCCATCGGt                                                           <  1:492272/250‑1 (MQ=255)
                                                                                                                                                                                               aTTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAaat                                                                                                                                                                                                              >  1:169750/1‑97 (MQ=255)
                                                                                                                                                                                               aTTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACTCCGCAGTATTTAGCGGATTTCATGAGCTGGGGTGCCATCGGTGCAAGaa                                                    <  1:186730/251‑1 (MQ=255)
                                                                                                                                                                                               aTTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTATTTAGCGGATTTCATGAGCTGGGGTGCCATCGGTGCAAGaa                                                    <  1:125158/251‑1 (MQ=255)
                                                                                                                                                                                                   aaaGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGAt                                                                                                         <  1:546020/194‑1 (MQ=255)
                                                                                                                                                                                                       aTACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTATTTAGc                                                                                       <  1:224733/208‑1 (MQ=255)
                                                                                                                                                                                                         aCCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTATTTAGCGGATTTCATGAGCTGGGGTGCCATCGGTGCAAGAACCACCGAAt                                           >  1:866898/1‑250 (MQ=255)
                                                                                                                                                                                                          ccTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAaataa                                                                                                                                                                                                            <  1:269120/88‑1 (MQ=255)
                                                                                                                                                                                                          ccTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGcc                                                                                                                                                                                                  <  1:214930/98‑1 (MQ=255)
                                                                                                                                                                                                          ccTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGTTTTGCGTATCGCGCGTATATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGTATATTCTCACACCGCAGTATTTCGCGGATTTCATGATCTGGGGTGCCATCTGTGCAAGCACCACCGTATc                                          >  1:377796/1‑250 (MQ=255)
                                                                                                                                                                                                          ccTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCacac                                                                                                    >  1:425998/1‑192 (MQ=255)
                                                                                                                                                                                                            ttACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTATTTAGCGGATTTCATGAGCTGGGGTGCCATCGGTGCAAGAACCACCGAATCCCa                                       >  1:275489/1‑251 (MQ=255)
                                                                                                                                                                                                                aaaTCATCATGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTAt                                                                                            >  1:393048/1‑194 (MQ=255)
                                                                                                                                                                                                                   tcatcaTGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTATTTAGCGGATTTCATGAGCTGGGGTGCCATCGGTGCAAGAACCACCGAATCCCAAGTacac                                <  1:410574/251‑1 (MQ=255)
                                                                                                                                                                                                                    catcatGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTATTTAGCGGATTTCATGAGCTg                                                                        <  1:884805/210‑1 (MQ=255)
                                                                                                                                                                                                                     atcatGCGCGTGTATTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTATTTAGCGGATTTCATGAGCTGGGGTGCCATCGGTGCAAGAACCACCGAATCCCAAGTACACCg                              <  1:868826/251‑1 (MQ=255)
                                                                                                                                                                                                                                  aTTTTGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATCAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCg                                                                                                                                        <  1:646609/132‑1 (MQ=255)
                                                                                                                                                                                                                                     ttGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATcc                                                                                                                                                           >  1:753323/1‑110 (MQ=255)
                                                                                                                                                                                                                                      tGAAAAGCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTATTTAGCGGATTTCATGAGCTGGGGTGCCATCGGTGCAAGAACCACCGAATCCCAAGTACACCGTGAACTGGCTTCCGGtt             <  1:117908/251‑1 (MQ=255)
                                                                                                                                                                                                                                            gCCGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTAt                                                                                            <  1:648451/166‑1 (MQ=255)
                                                                                                                                                                                                                                             ccGCGTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTATTTAGCGGATTTCATGAGCTGGGGTGCCATCGGTGCAAGAACCACCGAATCCCAAGTACACCGTGAACTGGCTTCCGGTTTATcc        <  1:353075/249‑1 (MQ=255)
                                                                                                                                                                                                                                               gcgTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTAttt                                                                                          >  1:75923/1‑165 (MQ=255)
                                                                                                                                                                                                                                               gcgTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTAt                                                                                            <  1:6447/163‑1 (MQ=255)
                                                                                                                                                                                                                                               gcgTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTATTTAGCGGATTTCATGAGCTGGGGTGCCa                                                                <  1:771057/191‑1 (MQ=255)
                                                                                                                                                                                                                                               gcgTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTATTTAGCGGATTTCATGAGCTGGGGTGCCATCGGTGCAAGAACCACCGAATCCCAAGTACACCGTGAACTGGCTTCCGGTTTATCCTgcg    >  1:272914/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 gTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTAttt                                                                                          >  1:6353/1‑163 (MQ=255)
                                                                                                                                                                                                                                                 gTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTATTTAGCGGATTTCATGAGCTGGGGTGCCATCGGTGCAAGAac                                                   >  1:172133/1‑202 (MQ=255)
                                                                                                                                                                                                                                                 gTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTATTTAGCGGATTTCATGAGCTGGGGTGCCATCGGTGCAAGAACCACCGAATCCCAAGTACACCGTGAACTGGCTTCCGGTTTATCCTGcgcg  >  1:298053/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 gTACCACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTATTTAGCGGATTTCATGAGCTGGGGTGCCATCGGTGCAAGAACCACCGAATCCCAAGTACACCGTGAACTGGCTTCCGGTTTATCCTGcgcg  >  1:653668/1‑251 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      
CCCATCGCGTTATTAGAAAAATTTCCTGCCAGCGAGGTTGCGGTAAAAACCGTAGAACAAGCCCGCGAAGAAGCACACGACATTATTCATGGCAAAGATGATCGCCTGTTGGTGGTGATCGGGCCTTGCTCCATTCACGATCCGAAAGCGGCATTGGAATATGCGCAACGCATTAAATTAATGCGTGAAAAATTCAAAGATACCTTACAAATCATCATGCGCGTGTATTTTGAAAAGCCGCGTACTACCGTGGGTTGGAAAGGCTTAATTAATGACCCGCACTTAAATAATACTTATGCCTTGAACGACGGTTTGCGTATGGCGCGTAAATTATTATCCGACATTAACGATTTAACCGTGCCTTCCGCCGGTGAGTTTTTGGATATGATCACACCGCAGTATTTAGCGGATTTCATGAGCTGGGGTGCCATCGGTGCAAGAACCACCGAATCCCAAGTACACCGTGAACTGGCTTCCGGTTTATCCTGCGCG  >  1/714248‑714739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑