Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	703,521	T→C	H391R (CAC→CGC)	unknown ←	Oligopeptidase A (EC 3.4.24.70)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	703,521	0	T	C	100.0%	163.7 / NA	60	H391R (CAC→CGC)	unknown	Oligopeptidase A (EC 3.4.24.70)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/34);  total (26/34)

ATTCATGGAACAGGGTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGTGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAGGGCGCAATTCTTCATCGCTAATGGCATATAAATGTTGTTTTTGTTTCTCGCTGTAAAAGGACAAATCCCAAAGCTCAAGTGCGGTCAAATTATAGTGTGTTTTGCAGAAATCTTTTAATTCCTGTAATTC  >  1/703285‑703762                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                  aTTCATGGAACAGGGTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGc                                                                                                                                                                                                                                     <  1:147429/251‑1 (MQ=255)  ttCATGGAACAGGGTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGcc                                                                                                                                                                                                                                    <  1:826904/251‑1 (MQ=255)   tCATGGAACAGGGTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCa                                                                                                                                                                                                                                   >  1:321025/1‑251 (MQ=255)        gAACAGGGTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGttt                                                                                                                                                                                                                              >  1:623971/1‑251 (MQ=255)        gAACAGGGTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGttt                                                                                                                                                                                                                              >  1:589436/1‑251 (MQ=255)          aCAGGGTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTc                                                                                                                                                                                                                             >  1:143910/1‑250 (MQ=255)             gggTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGc                                                                                                                                                                                                                                     >  1:328607/1‑239 (MQ=255)             gggTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCcaca                                                                                                                                                                                                                         >  1:513107/1‑251 (MQ=255)                     aCTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGAc                                                                                                                                                                                                                  <  1:502433/250‑1 (MQ=255)                      cTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGt                                                                                                                                                                                                                >  1:673863/1‑251 (MQ=255)                         catcatGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCcac                                                                                                                                                                                                                          <  1:240589/238‑1 (MQ=255)                         catcatGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGtt                                                                                                                                                                                                               >  1:400215/1‑249 (MQ=255)                         catcatGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAACTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGtt                                                                                                                                                                                                               >  1:456373/1‑249 (MQ=255)                         catcatGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAACTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGtt                                                                                                                                                                                                               >  1:443635/1‑249 (MQ=255)                             atGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACgg                                                                                                                                                                                                         <  1:785238/251‑1 (MQ=255)                              tGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGc                                                                                                                                                                                                                                     <  1:248984/222‑1 (MQ=255)                                     ataatGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATg                                                                                                                                                                                                 <  1:589362/251‑1 (MQ=255)                                           ccGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCGTCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGaa                                                                                                                                                                                            <  1:655478/250‑1 (MQ=255)                                                       cGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATc                                                                                                                                                                               <  1:245160/251‑1 (MQ=255)                                                             gACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCcac                                                                                                                                                                                                                          >  1:598897/1‑202 (MQ=255)                                                                        ttACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGtt                                                                                                                                                              >  1:534925/1‑251 (MQ=255)                                                                             ggTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGa                                                                                                                                                         >  1:94792/1‑251 (MQ=255)                                                                             ggTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGa                                                                                                                                                         >  1:727509/1‑251 (MQ=255)                                                                              gTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGa                                                                                                                                                         >  1:362822/1‑250 (MQ=255)                                                                                              gTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATg                                                                                                                                                                                        <  1:40345/203‑1 (MQ=255)                                                                                              gTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCggg                                                                                                                                           >  1:719311/1‑248 (MQ=255)                                                                                                  ttGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATATAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAg                                                                                                                                    <  1:448039/251‑1 (MQ=255)                                                                                                  ttGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAg                                                                                                                                    >  1:582881/1‑251 (MQ=255)                                                                                                           aCCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAGGGCGCAAtt                                                                                                                           >  1:659145/1‑251 (MQ=255)                                                                                                               tCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAGGGCGCAAttcttc                                                                                                                       <  1:150491/251‑1 (MQ=255)                                                                                                                               gacCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAGGGCGCAATTCTTCATCGCTAATg                                                                                                             >  1:591656/1‑245 (MQ=255)                                                                                                                                  cGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAGGGCGCAATTCTTCATCGCTAATGGCATATaaa                                                                                                    <  1:841852/251‑1 (MQ=255)                                                                                                                                     tACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAGGGCGCAATTCTTCATCGCTAATGGCATATAAAtgt                                                                                                 <  1:60854/251‑1 (MQ=255)                                                                                                                                         aTCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAGGGCGCAATTCTTCATCGCTAATgg                                                                                                            >  1:52550/1‑236 (MQ=255)                                                                                                                                         aTCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAGGGCGCAATTCTTCATCGCTAATGGCATATAAATgttgtt                                                                                             >  1:848699/1‑251 (MQ=255)                                                                                                                                          tCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAGGGCGCAATTCTTCATCGCTAATGGCATATAAATGttgttt                                                                                            <  1:419075/251‑1 (MQ=255)                                                                                                                                            gTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAGGGCGCAATTCTTCATCGCTAATGGCATATAAATGttgttttt                                                                                          >  1:108808/1‑251 (MQ=255)                                                                                                                                              ccatccatGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAGGGCGCAATTCTTCATCGCTAATGGCATATAAATGttgtttttgt                                                                                        <  1:416290/251‑1 (MQ=255)                                                                                                                                                atccatGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGCTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAGGGCGCAATTCTTCATCGCTAATGGCATATAAATGttgtttttgttt                                                                                      <  1:368836/251‑1 (MQ=255)                                                                                                                                                                 tttATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAGGGCGCAATTCTTCATCGCTAATGGCATATAAATGTTGTTTTTGTTTCTCGCTGTAAAAGGAc                                                                      <  1:472376/250‑1 (MQ=255)                                                                                                                                                                    aTGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGAt                                                                                                                                                   <  1:242502/170‑1 (MQ=255)                                                                                                                                                                       ttCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAGGGCGCAATTCTTCATCGCTAATGGCATATAAATGTTGTTTTTGTTTCTCGCTGTAAAAGGACAAATccc                                                               <  1:746442/251‑1 (MQ=255)                                                                                                                                                                           cGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGcc                                                                                                                                                                                                                                    >  1:2232/1‑82 (MQ=255)                                                                                                                                                                             ggCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGCAATAAGGGCGCAATTCTTCATCGCTAATGGCATATAAATGTTGTTTTTGTTTCTCGCTGTAAAAGGAc                                                                      >  1:88152/1‑238 (MQ=255)                                                                                                                                                                              gCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAGGGCGCAATTCTTCATCGCTAATGGCATATAAATGTTGTTTTTGTTTCTCGCTGTAAAAGGACAAATCCCAAAGCt                                                         <  1:82425/250‑1 (MQ=255)                                                                                                                                                                                     aaTCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAGTGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAGGGCGCAATTCTTCATCGCTAATGGCATATAAAtg                                                                                                  >  1:146067/1‑202 (MQ=255)                                                                                                                                                                                     aaTCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATc                                                                                                                                                                               <  1:581727/125‑1 (MQ=255)                                                                                                                                                                                     aaTCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATa                                                                                                                                                                     >  1:694555/1‑135 (MQ=255)                                                                                                                                                                                     aaTCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCtt                                                                                                                                                <  1:784477/156‑1 (MQ=255)                                                                                                                                                                                     aaTCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAGGGCGCAAttcttc                                                                                                                       <  1:114130/181‑1 (MQ=255)                                                                                                                                                                                                   ctgctgCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAGGGCGCAATTCTTCATCGCTAATGGCATATAAATGTTGTTTTTGTTTCTCGCTGTAAAAGGACAAATCCCAAAGCTCAAGTGCGGTCAAATTATAg                                     <  1:493236/249‑1 (MQ=255)                                                                                                                                                                                                   cTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAGGGCGCAATTCTTCATCGCTAATGGCATATAAATGTTGTTTTTGTTTCTCGCTGTAAAAGGACAAATCCCAAAGCTCAAGTGCGGTCAAATTATAgtg                                   <  1:794646/251‑1 (MQ=255)                                                                                                                                                                                                   cTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAGGGCGCAATTCTTCATCGCTAATGGCATATAAATGTTGTTTTTGTTTCTCGCTGTAAAAGGACAAATCCCAAAGCTCAAGTGCGGTCAAATTATAgtg                                   <  1:805726/251‑1 (MQ=255)                                                                                                                                                                                                   cTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAGGGCGCAATTCTTCATCGCTAATGGCATATAAATGTTGTTTTTGTTTCTCGCTGTAAAAGGACAAATCCCAAAGCTCAAGTGCGGTCAAATTATAgtg                                   <  1:395977/251‑1 (MQ=255)                                                                                                                                                                                                   cTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAGGGCGCAATTCTTCATCGCTAATGGCATATAAATGTTGTTTTTGTTTCTCGCTGTAAAAGGACAAATCCCAAAGCTCAAGTGCGGTCAAATTATAgtg                                   <  1:220033/251‑1 (MQ=255)                                                                                                                                                                                                         cgAACTTCATCATTTTCATCAATTAAGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAGGGCGCAATTCTTCATCGCTAATGGCATATAAATGTTGTTTTTGTTTCTCGCTGTAAAAGGACAAATCCCAAAGCTCAAGTGCGGTCAAATTATAGTGTGttt                              <  1:250915/250‑1 (MQ=255)                                                                                                                                                                                                                                 aaGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAGGGCGCAAttcttc                                                                                                                       <  1:264766/137‑1 (MQ=255)                                                                                                                                                                                                                                  aGTCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAGGGCGCAATTCTTCATCGCTAATGGCATATAAATGTTGTTTTTGTTTCTCGCTGTAAAAg                                                                         <  1:28228/182‑1 (MQ=255)                                                                                                                                                                                                                                    tCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAGGGCGCAATTCTTCATCGCTAATGGCATATAAATGTTGTTTTTGTTTCTCGCTGTAAAAGGACAAATCCCAAAGCTCAAGTGCGGTCAAATTATAGTGTGTTTTGCAGAAATCTTTTAATTCCTGTAAtt   <  1:312525/250‑1 (MQ=255)                                                                                                                                                                                                                                    tCAAAGAAGCGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTGCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAGGGCGCAATTCTTCATCGCTAATGGCATATAAATGTTGTTTTTGTTTCTCGCTGTAAAAGGACAAATCCCAAAGCTCAAGTGCGGTCAAATTATAGTGTGTTTTGCAGAAATCTTTTAATTCCTGTAATTc  <  1:434373/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                  ATTCATGGAACAGGGTAGTGACTTCATCATGGGTGAATAATGCCGGTTGATCACCGAGCGGACGATTGAAGTTACAGGTTAAATAGGCGACCGGTTTTTGTAGACTACCGTCGATGGTTTTGCGACGACCGATACAATCGTCCATCCATGCGCCGCCGCGTTTATGTTCACGGGCATACAAATCTAAATAGAAGCTGCCGCGAACTTCATCATTTTCATCAATTAAGTCAAAGAAGTGCACATCTTTATGCCAGGTTTCCACATCATGACGTTCTACGGCACGAATGTTGAAAATGCGTTTAATCAGTTCAAATAAGCCGTTAAGGACACGATCTTCCGGGAAATAAGGGCGCAATTCTTCATCGCTAATGGCATATAAATGTTGTTTTTGTTTCTCGCTGTAAAAGGACAAATCCCAAAGCTCAAGTGCGGTCAAATTATAGTGTGTTTTGCAGAAATCTTTTAATTCCTGTAATTC  >  1/703285‑703762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑