BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	64,778	A→G	E201G (GAG→GGG)	unknown →	Peptidoglycan D,D‑transpeptidase MrdA (EC 3.4.16.4)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	64,778	0	A	G	100.0%	403.1 / NA	143	E201G (GAG→GGG)	unknown	Peptidoglycan D,D‑transpeptidase MrdA (EC 3.4.16.4)
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (70/73); total (70/73)

Only 100 of 143 total aligned reads displayed.

ACTGATGAAGATATTGCGAATTTTAACAAGGAACGGCGTCGCTCCTCCCGTTATATGCCGATTATGCTGAAACGAAATCTAACGGAAGAGCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGAGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGACCATTGACTTAAATTTACAGCTTTACGTTACCGATTTATTGGCGGGACACAAAGGCGCCGTGGTAG  >  1/64534‑65023

 cTGATGAAGATATTGCGAATTTTAACAAGGAACGGCGTCGCTCCTCCCGTTATATGCCGATTATGCTGAAACGAAATCTAACGGAAGAGCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAg                                                                                                                                                                                                                                                   <  1:5254/251‑1 (MQ=255)

   gatgaAGATATTGCGAATTTTAACAAGGAACGGCGTTGCTCCTCCCGTTATCTGCCGATTATGCTGAAACGAAATCTAACGGAAGAGCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGACGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTa                                                                                                                                                                                                                                                 >  1:446650/1‑251 (MQ=255)

   gatgaAGATATTGCGAATTTTAACAAGGAACGGCGTCGCTCCTCCCGTTATATGCCGATTATGCTGAAACGAAATCTAACGGAAGAGCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGACGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTa                                                                                                                                                                                                                                                 >  1:433359/1‑251 (MQ=255)

                              gAACGGCGTCGCTCCTCCCGTTATATGCCGATTATGCTGAAACGAAATCTAACGGAAGAGCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGa                                                                                                                                                                                                                         >  1:807984/1‑248 (MQ=255)

                              gAACGGCGTCGCTCCTCCCGTTATATGCCGATTATGCTGAAACGAAATCTAACGGAAGAGCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGa                                                                                                                                                                                                                         >  1:26509/1‑248 (MQ=255)

                                 cGGCGTCGCTCCTCCCGTTATATGCCGATTATGCTGAAACGAAATCTAACGGAAGAGCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCTGGCACGCACGACTgggg                                                                                                                                                                                                                   <  1:513334/251‑1 (MQ=255)

                                           cctccCGTTATATGCCGATTATGCTGAAACGAAATCTAACGGAAGAGCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCgg                                                                                                                                                                                                          >  1:240215/1‑250 (MQ=255)

                                            ctccCGTTATATGCCGATTATGCTGAAACGAAATCTAACGGAAGAGCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCa                                                                                                                                                                                                        >  1:106976/1‑251 (MQ=255)

                                             tccCGTTATATGCCGATTATGCTGAAACGAAATCTAACGGAAGAGCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCAt                                                                                                                                                                                                       <  1:192239/251‑1 (MQ=255)

                                                 gTTATATGCCGATTATGCTGAAACGAAATCTAACGGAAGAGCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAg                                                                                                                                                                                                   >  1:148093/1‑251 (MQ=255)

                                                  ttATATGCCGATTATGCTGAAACGAAATCTAACGGAAGAGCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAgc                                                                                                                                                                                                  <  1:310319/251‑1 (MQ=255)

                                                      atGCCGATTATGCTGAAACGAAATCTAACGGAAGAGCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCta                                                                                                                                                                                              >  1:557708/1‑251 (MQ=255)

                                                           gATTATGCTGAAACGAAATCTAACGGAAGAGCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATg                                                                                                                                                                                         >  1:57792/1‑251 (MQ=255)

                                                            aTTATGCTGAAACGAAATCTAACGGAAGAGCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATg                                                                                                                                                                                         <  1:666690/250‑1 (MQ=255)

                                                            aTTATGCTGAAACGAAATCTAACGGAAGAGCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGa                                                                                                                                                                                        <  1:661946/251‑1 (MQ=255)

                                                            aTTATGCTGAAACGAAATCTAACGGAAGAGCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGa                                                                                                                                                                                        <  1:405296/251‑1 (MQ=255)

                                                            aTTATGCTGAAACGAAATCTAACGGAAGAGCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGa                                                                                                                                                                                        <  1:630815/251‑1 (MQ=255)

                                                            aTTATGCTGAAACGAAATCTAACGGAAGAGCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGa                                                                                                                                                                                        <  1:490784/251‑1 (MQ=255)

                                                              tATGCTGAAACGAAATCTAACGGAAGAGCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAg                                                                                                                                                                                      <  1:831920/251‑1 (MQ=255)

                                                              tATGCTGAAACGAAATCTAACGGAAGAGCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAg                                                                                                                                                                                      <  1:673729/251‑1 (MQ=255)

                                                                       aCGAAATCTAACGGAAGAGCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTa                                                                                                                                                                              >  1:827754/1‑250 (MQ=255)

                                                                             tCTAACGGAAGAGCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGc‑a                                                                                                                                                                      >  1:785440/1‑251 (MQ=255)

                                                                                 aCGGAAGAGCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGTGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGc‑accac                                                                                                                                                                  <  1:265879/251‑1 (MQ=255)

                                                                                 aCGGAAGAGCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGc‑accac                                                                                                                                                                  <  1:279322/251‑1 (MQ=255)

                                                                                    gAAGAGCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTCCGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAg                                                                                                                                                                                      >  1:425180/1‑229 (MQ=255)

                                                                                     aaGAGCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCGTTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAgg                                                                                                                                                               <  1:651125/250‑1 (MQ=255)

                                                                                       gagCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTacac                                                                                                                                                                           >  1:274862/1‑237 (MQ=255)

                                                                                        agCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCtatta                                                                                                                                                                                           <  1:784281/220‑1 (MQ=255)

                                                                                        agCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGa                                                                                                                                                                                        <  1:520752/223‑1 (MQ=255)

                                                                                           aaaTTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTAC‑TTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTgaag                                                                                                                                                       <  1:610976/251‑1 (MQ=255)

                                                                                                    gTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGa                                                                                                                                                                                        >  1:508623/1‑211 (MQ=255)

                                                                                                    gTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAagaagtagaa                                                                                                                                               >  1:577661/1‑251 (MQ=255)

                                                                                                    gTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAagaagtagaa                                                                                                                                               >  1:135580/1‑251 (MQ=255)

                                                                                                            gTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAg                                                                                                                                       >  1:807212/1‑251 (MQ=255)

                                                                                                              gAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAgc                                                                                                                                                                                                  >  1:208758/1‑191 (MQ=255)

                                                                                                              gAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAg                                                                                                                                                                                      <  1:143784/203‑1 (MQ=255)

                                                                                                               aaTCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATg                                                                                                                                                                                         >  1:74060/1‑199 (MQ=255)

                                                                                                               aaTCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTcg                                                                                                                                    >  1:590410/1‑251 (MQ=255)

                                                                                                                       cAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGAGCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAg                                                                                                                           <  1:265287/251‑1 (MQ=255)

                                                                                                                        aaTTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGa                                                                                                                                                                                        >  1:278999/1‑191 (MQ=255)

                                                                                                                        aaTTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGt                                                                                                                          <  1:779429/251‑1 (MQ=255)

                                                                                                                           ttCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGa                                                                                                                                                                                        >  1:156353/1‑188 (MQ=255)

                                                                                                                                  gTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGgca                                                                                                                                                                                                                                >  1:272761/1‑141 (MQ=255)

                                                                                                                                  gTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTg                                                                                                                         <  1:71367/242‑1 (MQ=255)

                                                                                                                                        aTGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGcc                                                                                                                                                                                                                                             <  1:620676/122‑1 (MQ=255)

                                                                                                                                         tgtgAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTgcgcg                                                                                                        >  1:178118/1‑251 (MQ=255)

                                                                                                                                            gAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGca                                                                                                      >  1:695977/1‑250 (MQ=255)

                                                                                                                                               aCCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAg                                                                                                                                                                                      <  1:481941/170‑1 (MQ=255)

                                                                                                                                                  cTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCg                                                                                               >  1:737942/1‑251 (MQ=255)

                                                                                                                                                    aCTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTgaag                                                                                                                                                       >  1:458137/1‑195 (MQ=255)

                                                                                                                                                    aCTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGc                                                                                              >  1:98546/1‑250 (MQ=255)

                                                                                                                                                    aCTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGc                                                                                              >  1:170775/1‑250 (MQ=255)

                                                                                                                                                    aCTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGc                                                                                              >  1:382550/1‑250 (MQ=255)

                                                                                                                                                              ccATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCa                                                                                   >  1:557198/1‑251 (MQ=255)

                                                                                                                                                                attattTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGc                                                                                              <  1:17539/238‑1 (MQ=255)

                                                                                                                                                                 ttatttatACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTgaag                                                                                                                                                       >  1:98210/1‑182 (MQ=255)

                                                                                                                                                                 ttatttatACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCaaaa                                                                                >  1:404630/1‑251 (MQ=255)

                                                                                                                                                                  tatttatACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAg                                                                                                                                                                >  1:727378/1‑172 (MQ=255)

                                                                                                                                                                   atttatACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTa                                                                                                                              <  1:543270/204‑1 (MQ=255)

                                                                                                                                                                             cGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGA‑‑AAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGAcc                                                                  <  1:876951/251‑1 (MQ=255)

                                                                                                                                                                               aaCCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGAcc                                                                  <  1:11007/251‑1 (MQ=255)

                                                                                                                                                                               aaCCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGAcc                                                                  <  1:672579/251‑1 (MQ=255)

                                                                                                                                                                                 ccGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAgc                                                                                                                                                                                                  >  1:512095/1‑124 (MQ=255)

                                                                                                                                                                                 ccGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGACCAt                                                                <  1:318602/251‑1 (MQ=255)

                                                                                                                                                                                       aCCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGACCATTGACt                                                           <  1:757223/250‑1 (MQ=255)

                                                                                                                                                                                           aTGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGACCATTGACTTAAAt                                                      <  1:363437/251‑1 (MQ=255)

                                                                                                                                                                                             gTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGAc                                                                                                                                                                                                                        <  1:337699/90‑1 (MQ=255)

                                                                                                                                                                                             gTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTgggg                                                                                                                                                                                                                   >  1:737518/1‑95 (MQ=255)

                                                                                                                                                                                             gTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTg                                                                                                            <  1:80173/195‑1 (MQ=255)

                                                                                                                                                                                              ttttGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGACCATTGACTTAAAttt                                                    <  1:140232/250‑1 (MQ=255)

                                                                                                                                                                                                 tGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGACCATTGACTTAAATTTACAg                                                <  1:892029/251‑1 (MQ=255)

                                                                                                                                                                                                         gtgtCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAAc                                                                                                                                         <  1:178356/156‑1 (MQ=255)

                                                                                                                                                                                                          tgtCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGACCATTGACTTAAATTTACAGCTTTACGt                                        <  1:566892/250‑1 (MQ=255)

                                                                                                                                                                                                                aaaTTAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTg                                                                                                                                                                                                     <  1:626261/90‑1 (MQ=255)

                                                                                                                                                                                                                aaaTCAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGACCATTGACTTAAATTTACAGCTTTACGTTACCGAt                                 >  1:727507/1‑251 (MQ=255)

                                                                                                                                                                                                                   ttAACGATCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGACCATTGACTTAAATTTACAGCTTTACGTTACCGATTTa                              <  1:677163/251‑1 (MQ=255)

                                                                                                                                                                                                                         aTCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAagaagtagaag                                                                                                                                              <  1:118139/135‑1 (MQ=255)

                                                                                                                                                                                                                         aTCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGACCATTGACTTAAATTTACAGCtt                                             >  1:143038/1‑230 (MQ=255)

                                                                                                                                                                                                                         aTCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGACCATTGACTTAAATTTACAGCTTTACGTTACCGATTTATTGGc                         <  1:176497/250‑1 (MQ=255)

                                                                                                                                                                                                                         aTCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGACCATTGACTTAAATTTACAGCTTTACGTTACCGATTTATTGGc                         <  1:888702/250‑1 (MQ=255)

                                                                                                                                                                                                                          tCGTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGACCATTGACTTAAATTTACAGCTTTACGTTACCGATTTATTGGCgg                       >  1:681924/1‑251 (MQ=255)

                                                                                                                                                                                                                            gTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGACCATTGACTTAAATTTACAGCTTTACGTTACCGATTTATTGGCGGGa                     >  1:854365/1‑251 (MQ=255)

                                                                                                                                                                                                                            gTGATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTAACA‑GGCAACCAAAGCCTTTGAAGAAGAGGAAGGGAACAG‑CGCCGGAAAAG‑GAACCGCCAAATTCGGCCAACAACCCCCCATTCCCGGCAAAAGAATCAACTTGCCAATTGACTAAAATTAACACCTTAACGTAACCGAttaatggccggga                     >  1:638526/1‑240 (MQ=255)

                                                                                                                                                                                                                              gATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTcc                                                                                                                                                                                                                                    >  1:770652/1‑45 (MQ=255)

                                                                                                                                                                                                                              gATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGACCATTGACTTAAATTTACAGCTTTACGt                                        <  1:547704/230‑1 (MQ=255)

                                                                                                                                                                                                                              gATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGACCATTGACTTAAATTTACAGCTTTACGTTACCGATTTATTGGCGGGaca                   >  1:184465/1‑251 (MQ=255)

                                                                                                                                                                                                                              gATGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGACCATTGACTTAAATTTACAGCTTTACGTTACCGATTTATTGGCGGGaca                   >  1:130476/1‑251 (MQ=255)

                                                                                                                                                                                                                               aTGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGa                                                                                                                                                                                        >  1:225722/1‑88 (MQ=255)

                                                                                                                                                                                                                               aTGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGa                                                                                                                                                                                        >  1:700282/1‑88 (MQ=255)

                                                                                                                                                                                                                               aTGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGACCATTGACTTAAATTTACAGCTTTACGTTACCGATTTATTGGCGGGacac                  <  1:468697/251‑1 (MQ=255)

                                                                                                                                                                                                                               aTGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGACCATTGACTTAAATTTACAGCTTTACGTTACCGATTTATTGGCGGGaca                   >  1:796874/1‑250 (MQ=255)

                                                                                                                                                                                                                               aTGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGACCATTGACTTAAATTTACAGCTTTACGTTACCGATTTATTGGCGGGaca                   >  1:656225/1‑250 (MQ=255)

                                                                                                                                                                                                                               aTGTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGACCATTGACTTAAATTTACAGCTTTACGTTACCGATTTATTGGCGGGaca                   >  1:640774/1‑250 (MQ=255)

                                                                                                                                                                                                                                 gTAGAACGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGACCATTGACTTAAATTTACAGCTTTACGTTACCGATTTATTGGCGGGACACaa                >  1:879436/1‑251 (MQ=255)

                                                                                                                                                                                                                                     aaCGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGACCATTGACTTAAATTTACAGCTTTACGTTACCGATTTATTGGCGGGACACAAAgg             <  1:146086/250‑1 (MQ=255)

                                                                                                                                                                                                                                     aaCGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGACCATTGACTTAAATTTACAGCTTTACGTTACCGATTTATTGGCGGGACACAAAgg             <  1:758470/250‑1 (MQ=255)

                                                                                                                                                                                                                                     aaCGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGACCATTGACTTAAATTTACAGCTTTACGTTACCGATTTATTGGCGGGACACAAAGgc            <  1:789693/251‑1 (MQ=255)

                                                                                                                                                                                                                                     aaCGCTTGAAAAAAGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGACCATTGACTTAAATTTACAGCTTTACGTTACCGATTTATTGGCGGGACACAAAGgc            <  1:70618/251‑1 (MQ=255)

                                                                                                                                                                                                                                               aaaaGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGACCATTGACTTAAATTTACAGCTTTACGTTACCGATTTATTGGCGGGACACAAAGGCGCCGTGGTAg  <  1:70448/251‑1 (MQ=255)

                                                                                                                                                                                                                                               aaaaGGGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGACCATTGACTTAAATTTACAGCTTTACGTTACCGATTTATTGGCGGGACACAAAGGCGCCGTGGTAg  <  1:714819/251‑1 (MQ=255)

ACTGATGAAGATATTGCGAATTTTAACAAGGAACGGCGTCGCTCCTCCCGTTATATGCCGATTATGCTGAAACGAAATCTAACGGAAGAGCAAATTGCCCGTTTTGCGGTGAATCAATACAATTTCCAGAGTTTGGATGTGAAACCCTACTTTAAGCGCCATTATTTATACGGCGAACCGCTGACCCATGTTTTGGGCTATGTGTCAAAAATTAACGATCGTGATGTAGAACGCTTGAAAAAAGAGGAAAAGTACGCCAATTATTCCGGCACGCACGACTGGGGCAAACTCGGCATTGAGCGCTATTATGAAGATGCTTTACACGGC‑ACCACAGGCTTTGAAGAAGTAGAAGTGAACAGTCG‑CGGTAAAGTGATCCG‑CAAATTGCGCGCACAACCCGCCATTGCCGGCAAAAGCATTCACTTGACCATTGACTTAAATTTACAGCTTTACGTTACCGATTTATTGGCGGGACACAAAGGCGCCGTGGTAG  >  1/64534‑65023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`