Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	697,419	C→T	K54K (AAG→AAA)	unknown ←	Dca

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	697,419	0	C	T	100.0%	10.7 / NA	6	K54K (AAG→AAA)	unknown	Dca
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/3);  total (3/3)

GGTTTCCGGTATTGCTTGTTTTCTTAGTCTATATAAAATATATCCGGATAAAATAAATAAGAATGGCAGTAAAAATTGGCTAAATTTTAATTTTCCTAAAAATTCAAAAGATTCTTCCGGGTTGGTTTCAAAAAAAGCAGCAACAACTCCGGAGTTTAAGTTTCCATAAGATAAATATATAGGCTGATAAATAGATAACGTTATGATAACGAATGTAATAAGGCTCCAGAATAACTTCTGATTTAGTGAGAATATTAAATAATAGATAATGTATATTACAAACATGCCATAATAAATAGTATTCTCAGGTGATATAATAAGCGTAATAAATGAGAAAAAGAGTAGCCAAATCCAAAAGATTCGGCTATTTAGATAATTAAGAAATTTTTTAAACATAAATTGGTTCTTTAGGTTTAAGACTGAAGATAACAGAAAAGACAAAGTAAGAGTGCTAATTTTATACCTA  >  1/697185‑697650                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                        ggTTTCCGGTATTGCTTGTTTTCTTAGTCTATATAAAATATATCCGGATAAAATAAATAAGAATGGCAGTAAAAATTGGCTAAATTTTAATTTTCCTAAAAATTCAAAAGATTCTTCCGGGTTGGTTTCAAAAAAAGCAGCAACAACTCCGGAGTTTAAGTTTCCATAAGATAAATATATAGGCTGATAAATAGATAACGTTATGATAACGAATGTAATAAGGCTCCAGAATAATTTCTGATTTAGTgaga                                                                                                                                                                                                                         >  1:503500/1‑251 (MQ=255)                     ttCTTAGTCTATATAAAATATATCCGGATAAAATAAATAAGAATGGCAGTAAAAATTGGCTAAATTTTAATTTTCCTAAAAATTCAAAAGATTCTTCCGGGTTGGTTTCAAAAAAAGCAGCAACAACTCCGGAGTTTAAGTTTCCATAAGATAAATATATAGGCTGATAAATAGATAACGTTATGATAACGAATGTAATAAGGCTCCAGAATAATTTCTGATTTAGTg                                                                                                                                                                                                                            <  1:562809/228‑1 (MQ=255)                                                                                                                                                                          gATAAATATATAGGCTGATAAATAGATAACGTTATGATAACGAATGTAATAAGGCTCCAGAATAATTTCTGATTTAGTGAGAATATTAAATAATAGATAATGTATATTACAAACATGCCATAATAAATAGTATTCTCAGGTGATATAATAAGCGTAATaaa                                                                                                                                          <  1:218636/161‑1 (MQ=255)                                                                                                                                                                                          gATAAATAGATAACGTTATGATAACGAATGTAATAAGGCTCCAGAATAATTTCTGATTTAGTGAGAATATTAAATAATAGATAATGTATATTACAAACATGCCATAATAAATAGTATTCTCAGGTGATATAATAAGCGTAATAAATGAGAAAAAGAGTAGCCAAATCCAAAAGATTCGGCTATTTAGATAATTAAGAAATTTTTTAAACATAAATTGGTTCTTTAGGTTTAAGACTGAAGATAACAGaaaa                                >  1:694532/1‑251 (MQ=255)                                                                                                                                                                                            tAAATAGATAACGTTATGATAACGAATGTAATAAGGCTCCAGAATAATTTCTGATTTAGTGAGAATATTAAATAATAGAt                                                                                                                                                                                                         <  1:429791/80‑1 (MQ=255)                                                                                                                                                                                                                        taataaGGCTCCAGAATAATTTCTGATTTAGTGAGAATATTAAATAATAGATAATGTATATTACAAACATGCCATAATAAATAGTATTCTCAGGTGATATAATAAGCGTAATAAATGAGAAAAAGAGTAGCCAAATCCAAAAGATTCGGCTATTTAGATAATTAAGAAATTTTTTAAACATAAATTGGTTCTTTAGGTTTAAGACTGAAGATAACAGAAAAGACAAAGTAAGAGTGCTAATTTTATACCta  >  1:540484/1‑251 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                        GGTTTCCGGTATTGCTTGTTTTCTTAGTCTATATAAAATATATCCGGATAAAATAAATAAGAATGGCAGTAAAAATTGGCTAAATTTTAATTTTCCTAAAAATTCAAAAGATTCTTCCGGGTTGGTTTCAAAAAAAGCAGCAACAACTCCGGAGTTTAAGTTTCCATAAGATAAATATATAGGCTGATAAATAGATAACGTTATGATAACGAATGTAATAAGGCTCCAGAATAACTTCTGATTTAGTGAGAATATTAAATAATAGATAATGTATATTACAAACATGCCATAATAAATAGTATTCTCAGGTGATATAATAAGCGTAATAAATGAGAAAAAGAGTAGCCAAATCCAAAAGATTCGGCTATTTAGATAATTAAGAAATTTTTTAAACATAAATTGGTTCTTTAGGTTTAAGACTGAAGATAACAGAAAAGACAAAGTAAGAGTGCTAATTTTATACCTA  >  1/697185‑697650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑