Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	694,410	G→A	L305L (CTG→TTG)	unknown ←	Spermidine synthase long (EC 2.5.1.16)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	694,410	0	G	A	100.0%	124.9 / NA	47	L305L (CTG→TTG)	unknown	Spermidine synthase long (EC 2.5.1.16)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/19);  total (28/19)

AAGTAAATTAGATGCATAATTTCTCCAATGGAATGTGGTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGTAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAGTTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGACTGTGATTCAAATTTGTATTTAGCATACCATCATAAGCATTACCGCCGAATACGAGTTTGTCATTGTTTTCGCTTAAATATACTTGAATAAAACCGTGTTTATTTTCAATCAGTTTCTCTAA  >  1/694201‑694650                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 aaGTAAATTAGATGCATAATTTCTCCAATGGAATGTGGTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTa                                                                                                                                                                                                          >  1:559951/1‑250 (MQ=255)            aTGCATAATTTCTCCAATGGAATGTGGTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAAcc                                                                                                                                                                                                  <  1:771525/247‑1 (MQ=255)                       ctcCAATGGAATGTGGTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGAt                                                                                                                                                                                                                                     >  1:13694/1‑201 (MQ=255)                       ctcCAATGGAATGTGGTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGtt                                                                                                                                                                                   >  1:39516/1‑251 (MQ=255)                              ggAATGTGGTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAAc                                                                                                                                                                                                   >  1:439598/1‑228 (MQ=255)                                   gtgGTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAagag                                                                                                                                                                                                                >  1:501380/1‑210 (MQ=255)                                   gtgGTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGt                                                                                                                                                                                    <  1:566947/238‑1 (MQ=255)                                     ggTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTAc                                                                                                                                                                                                         <  1:247419/215‑1 (MQ=255)                                        aTTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCaa                                                                                                                                                                  >  1:20262/1‑251 (MQ=255)                                         ttCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAAt                                                                                                                                                                 <  1:128922/251‑1 (MQ=255)                                           cATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATaa                                                                                                                                                               >  1:48246/1‑251 (MQ=255)                                                gAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGt                                                                                                                                                          >  1:872801/1‑251 (MQ=255)                                                gAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGt                                                                                                                                                          >  1:59922/1‑251 (MQ=255)                                                       gTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTaataaat                                                                                                                                                   >  1:504132/1‑251 (MQ=255)                                                        tCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTaataaat                                                                                                                                                   <  1:353445/250‑1 (MQ=255)                                                                       ggATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAAc                                                                                                                                                              >  1:218803/1‑224 (MQ=255)                                                                       ggATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATg                                                                                                                                     >  1:748927/1‑249 (MQ=255)                                                                       ggATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATg                                                                                                                                     >  1:669047/1‑249 (MQ=255)                                                                       ggATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGc                                                                                                                                    >  1:602090/1‑250 (MQ=255)                                                                        gATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCa                                                                                                                                  >  1:514974/1‑251 (MQ=255)                                                                          ttACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAAc                                                                                                                                                              >  1:534541/1‑221 (MQ=255)                                                                                tttAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGAc                                                                                                                           <  1:786955/250‑1 (MQ=255)                                                                                 ttAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGa                                                                                                                            >  1:252451/1‑248 (MQ=255)                                                                                   aaCCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAAcc                                                                                                                                                             <  1:482109/213‑1 (MQ=255)                                                                                   aaCCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGAc                                                                                                                           <  1:498748/247‑1 (MQ=255)                                                                                                      caGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGACTGTGATTCAAATTTGTATTTAg                                                                                                     >  1:481973/1‑250 (MQ=255)                                                                                                        gTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTc                                                                                                                                         >  1:209845/1‑212 (MQ=255)                                                                                                        gTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGACTGTGATTCAAATTTGTAttt                                                                                                       <  1:133504/246‑1 (MQ=255)                                                                                                         tgatgaGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATCCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGACTGTGATTCAAATTTGTATTTAGc                                                                                                    <  1:49380/248‑1 (MQ=255)                                                                                                              agaTTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTa                                                                                                                               >  1:99355/1‑216 (MQ=255)                                                                                                                  tGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGACTGTGATTCAAATTTGTATTTAGCATACCATCATaa                                                                                        <  1:527250/251‑1 (MQ=255)                                                                                                                                      aaTTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGACTGTGATTCAAATTTGTATTTAGCATACCATCATAAGCATTACCGCCGAATACGAg                                                                    <  1:367476/251‑1 (MQ=255)                                                                                                                                       aTTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGACTGTGATTCAAATTTGTA‑TTAGCATACCATCATAAGCATTACCGCCGAATACGAGtt                                                                  <  1:612233/251‑1 (MQ=255)                                                                                                                                                tttCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGACtg                                                                                                                         >  1:622997/1‑188 (MQ=255)                                                                                                                                                tttCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGACTGTGATTCAAATTTGTATTTAGCATACCATCATAAGCATTACCGCCGAATACGAGTTTGTCATTg                                                          >  1:588490/1‑251 (MQ=255)                                                                                                                                                tttCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGACTGTGATTCAAATTTGTATTTAGCATACCATCATAAGCATTACCGCCGAATACGAGTTTGTCATTg                                                          >  1:384118/1‑251 (MQ=255)                                                                                                                                                       gTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGACTGTGATTCAAATTTGTATTTAGCATACCATCATAAGCATTACCGCCGAATACGAGTTTGTCATTGTTTTCg                                                    >  1:615203/1‑250 (MQ=255)                                                                                                                                                               cGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGACTGTGATTCAAATTTGTATTTAGCATACCATCATAAGCATTACCGCCGAATACGAGTTTGTCATTGTTTTCGCTTAAatat                                           <  1:484032/251‑1 (MQ=255)                                                                                                                                                                  cTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGACTGTGATTCAAATTTGTATTTAGCATACCATCATAAGCATTACCGCCGAATACGAGTTTGTCATTGTTTTCGCTTAAATATACt                                        <  1:474471/251‑1 (MQ=255)                                                                                                                                                                          ggATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGACTGTGATTCAAATTTGTATTTAGCATACCATCATAAGCATTACCGCCGAATACGAGTTTGTCATTGTTTTCGCTTAAATATAc                                         >  1:302153/1‑242 (MQ=255)                                                                                                                                                                          ggATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGACTGTGATTCAAATTTGTATTTAGCATACCATCATAAGCATTACCGCCGAATACGAGTTTGTCATTGTTTTCGCTTAAATATACTTGAATaa                                 <  1:526512/250‑1 (MQ=255)                                                                                                                                                                                    gATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGACTGTGATTCAAATTTGTATTTAGCATACCATCATAAGCATTACCGCCGAATACGAGTTTGTCATTGTTTTCGCTTAAATATACTTGAATAAAACCGTGttt                       <  1:889318/250‑1 (MQ=255)                                                                                                                                                                                    gATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGACTGTGATTCAAATTTGTATTTAGCATACCATCATAAGCATTACCGCCGAATACGAGTTTGTCATTGTTTTCGCTTAAATATACTTGAATAAAACCGTGTTTa                      >  1:615966/1‑251 (MQ=255)                                                                                                                                                                                             cAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGACTGTGATTCAAATTTGTATTTAGCATACCATCATAAGc                                                                                      >  1:620552/1‑178 (MQ=255)                                                                                                                                                                                             cAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGACTGTGATTCAAATTTGTATTTAGCATACCATCATAAGc                                                                                      >  1:875750/1‑178 (MQ=255)                                                                                                                                                                                                       cATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTc                                                                                                                                         <  1:260470/117‑1 (MQ=255)                                                                                                                                                                                                        aTTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGACTGTGATTCAAATTTGTATTTAGCATACCATCATAAGCATTACCGCCGAATACGAGTTTGTCATTGTTTTCGCTTAAATATACTTGAATAAAACCGTGTTTATTTTCAATCAGTTTCTCTaa  <  1:109116/251‑1 (MQ=255)                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 AAGTAAATTAGATGCATAATTTCTCCAATGGAATGTGGTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGTAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAGTTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGACTGTGATTCAAATTTGTATTTAGCATACCATCATAAGCATTACCGCCGAATACGAGTTTGTCATTGTTTTCGCTTAAATATACTTGAATAAAACCGTGTTTATTTTCAATCAGTTTCTCTAA  >  1/694201‑694650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑