Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	694,330	T→C	L331L (CTA→CTG)	unknown ←	Spermidine synthase long (EC 2.5.1.16)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	694,330	0	T	C	100.0%	120.1 / NA	43	L331L (CTA→CTG)	unknown	Spermidine synthase long (EC 2.5.1.16)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/14);  total (29/14)

GTGTAGTATGCATCATATGATTCCGTTGTGTTGAAATAGATAAAACCATTTTCATTTAAATGCGATTTTGTTAATTCTAAAAACTCTTTACTAAGTAAATTAGATGCATAATTTCTCCAATGGAATGTGGTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGTAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAGTTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGACTGTGATTCAAATTTGTATTTAGCATACCATCATAA  >  1/694109‑694564                                                                                                                                                                                                                              |                                                                                                                                                                                                                                           gtgtAGTATGCATCATATGATTCCGTTGTGTTGAAATAGATAAAACCATTTTCATTTAAATGCGATTTTGTTAATTCTAAAAACTCTTTACTAAGTAAATTAGATGCATAATTTCTCCAATGGAATGTGGTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTc                                                                                                                                                                                                                >  1:549714/1‑250 (MQ=255)    tagtaTGCATCATATGATTCCGTTGTGTTGAAATAGATAAAACCATTTTCATTTAAATGCGATTTTGTTAATTCTAAAAACTCTTTACTAAGTAAATTAGATGCATAATTTCTCCAATGGAATGTGGTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGc                                                                                                                                                                                                            <  1:647801/251‑1 (MQ=255)     agtaTGCATCATATGATTCCGTTGTGTTGAAATAGATAAAACCATTTTCATTTAAATGCGATTTTGTTAATTCTAAAAACTCTTTACTAAGTAAATTAGATGCATAATTTCTCCAATGGAATGTGGTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCgg                                                                                                                                                                                                             >  1:172513/1‑249 (MQ=255)      gtaTGCATCATATGATTCCGTTGTGTTGAAATAGATAAAACCATTTTCATTTAAATGCGATTTTGTTAATTCTAAAAACTCTTTACTAAGTAAATTAGATGCATAATTTCTCCAATGGAATGTGGTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTc                                                                                                                                                                                                                           <  1:154206/234‑1 (MQ=255)               atatGATTCCGTTGTGTTGAAATAGATAAAACCATTTTCATTTAAATGCGATTTTGTTAATTCTAAAAACTCTTTACTAAGTAAATTAGATGCATAATTTCTCCAATGGAATGTGGTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGAt                                                                                                                                                                                                 >  1:364501/1‑251 (MQ=255)                                         tAAAACCATTTTCATTTAAATGCGATTTTGTTAATTCTAAAAACTCTTTACTAAGTAAATTAGATGCATAATTTCTCCAATGGAATGTGGTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTc                                                                                                                                                                       <  1:85988/251‑1 (MQ=255)                                           aaaCCATTTTCATTTAAATGCGATTTTGTTAATTCTAAAAACTCTTTACTAAGTAAATTAGATGCATAATTTCTCCAATGGAATGTGGTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCAt                                                                                                                                                                     >  1:395885/1‑251 (MQ=255)                                                                                             aaGTAAATTAGATGCATAATTTCTCCAATGGAATGTGGTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTa                                                                                                                    >  1:559951/1‑250 (MQ=255)                                                                                                        aTGCATAATTTCTCCAATGGAATGTGGTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAAcc                                                                                                            <  1:771525/247‑1 (MQ=255)                                                                                                         tGCATAATTTCTCCAATGGAATGTGGTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGccc                                                                                                                                                                                                                                >  1:341288/1‑130 (MQ=255)                                                                                                                   ctcCAATGGAATGTGGTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGAt                                                                                                                                               >  1:13694/1‑201 (MQ=255)                                                                                                                   ctcCAATGGAATGTGGTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGtt                                                                                             >  1:39516/1‑251 (MQ=255)                                                                                                                          ggAATGTGGTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAAc                                                                                                             >  1:439598/1‑228 (MQ=255)                                                                                                                               gtgGTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTc                                                                                                                                                                                                                >  1:252089/1‑124 (MQ=255)                                                                                                                               gtgGTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAAtt                                                                                                                                                                                  >  1:202199/1‑154 (MQ=255)                                                                                                                               gtgGTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAagag                                                                                                                          >  1:501380/1‑210 (MQ=255)                                                                                                                               gtgGTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGt                                                                                              <  1:566947/238‑1 (MQ=255)                                                                                                                                 ggTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTAc                                                                                                                   <  1:247419/215‑1 (MQ=255)                                                                                                                                    aTTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCaa                                                                            >  1:20262/1‑251 (MQ=255)                                                                                                                                     ttCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAAt                                                                           <  1:128922/251‑1 (MQ=255)                                                                                                                                       cATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATaa                                                                         >  1:48246/1‑251 (MQ=255)                                                                                                                                            gAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGt                                                                    >  1:872801/1‑251 (MQ=255)                                                                                                                                            gAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGt                                                                    >  1:59922/1‑251 (MQ=255)                                                                                                                                                   gTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTaataaat                                                             >  1:504132/1‑251 (MQ=255)                                                                                                                                                    tCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTaataaat                                                             <  1:353445/250‑1 (MQ=255)                                                                                                                                                                   ggATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAAc                                                                        >  1:218803/1‑224 (MQ=255)                                                                                                                                                                   ggATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATg                                               >  1:669047/1‑249 (MQ=255)                                                                                                                                                                   ggATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATg                                               >  1:748927/1‑249 (MQ=255)                                                                                                                                                                   ggATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGc                                              >  1:602090/1‑250 (MQ=255)                                                                                                                                                                    gATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCa                                            >  1:514974/1‑251 (MQ=255)                                                                                                                                                                      ttACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAAc                                                                        >  1:534541/1‑221 (MQ=255)                                                                                                                                                                           gTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAAtt                                                                                                                                                                                  >  1:269728/1‑110 (MQ=255)                                                                                                                                                                            tttAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGAc                                     <  1:786955/250‑1 (MQ=255)                                                                                                                                                                             ttAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGa                                      >  1:252451/1‑248 (MQ=255)                                                                                                                                                                               aaCCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAAcc                                                                       <  1:482109/213‑1 (MQ=255)                                                                                                                                                                               aaCCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGAc                                     <  1:498748/247‑1 (MQ=255)                                                                                                                                                                                                  caGTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGACTGTGATTCAAATTTGTATTTAg               >  1:481973/1‑250 (MQ=255)                                                                                                                                                                                                    gTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTc                                                                                                                                                                                 >  1:813702/1‑86 (MQ=255)                                                                                                                                                                                                    gTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTc                                                   >  1:209845/1‑212 (MQ=255)                                                                                                                                                                                                    gTGATGAGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGACTGTGATTCAAATTTGTAttt                 <  1:133504/246‑1 (MQ=255)                                                                                                                                                                                                     tgatgaGATTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATCCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGACTGTGATTCAAATTTGTATTTAGc              <  1:49380/248‑1 (MQ=255)                                                                                                                                                                                                          agaTTGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTa                                         >  1:99355/1‑216 (MQ=255)                                                                                                                                                                                                              tGACCCGCTTGTCCTGCAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAATTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGACTGTGATTCAAATTTGTATTTAGCATACCATCATaa  <  1:527250/251‑1 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                                           GTGTAGTATGCATCATATGATTCCGTTGTGTTGAAATAGATAAAACCATTTTCATTTAAATGCGATTTTGTTAATTCTAAAAACTCTTTACTAAGTAAATTAGATGCATAATTTCTCCAATGGAATGTGGTATTCATTAGAATAAAGTCAAATTTTTTCTCAGGATTACGGTTTAACCAACGGCGACCATCATCAGTGATGAGATTGACCCGCTTGTCCTGTAGAAATTTGCCCATTTCAGAGTACATTCCGGCTAGCTGAGGATAGCCCGGATTTAATTCAATAACAGTCATTGATTCCAGTTCGGGCATTGATGTTAAAACTCTTGTCCAAGAGGCTGTACTTAAACCGATAACAAGAATGTTTTTCGCATGAGGTGCAATAACCGGTAATAAATAAGCTCTTTCAATGCCATTATGACTGTGATTCAAATTTGTATTTAGCATACCATCATAA  >  1/694109‑694564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑