Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 692,467 C→T D10N (GAC→AAC)  unknown ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1692,4670CT100.0% 20.6 / NA 10D10N (GAC→AAC) unknownhypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/6);  total (4/6)

ACCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCACTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACTAATTTTTTTATGTCATTATTTATAAATAATTTATTATACATATTAACCTTTTTAGTGTTTAATTATTACTTATTATTTCGAAAAACAATATATCTGGAATATAAAAAACCTAGGATTAGGCTTATACCGGAAGAGGTTATTAGCGTAATTGCCGGGAGCGCTTCTATTTTATCGGCTATCAATCCTATGAGATAACTC  >  1/692251‑692651
                                                                                                                                                                                                                        |                                                                                                                                                                                        
aCCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACTAATTTTTTTATGTTATTATTTATAAATAATTTATTATACATATTAAc                                                                                                                                                         <  1:81424/250‑1 (MQ=255)
    ttGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACTAATTTTTTTATGTTATTATTTATAAATAATTTATTATACat                                                                                                                                                               >  1:538508/2‑240 (MQ=255)
    gTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACTAATTTTTTTATGTTATTATTTATAAATAATTTATTATACATATTAACCtttt                                                                                                                                                    >  1:252364/1‑251 (MQ=255)
                           ggTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACTAATTTTTTTATGTTATTATTTATAAATAATTTATTATACATATTAACCTTTTTAGTGTTTAATTATTACttatt                                                                                                                              <  1:291422/250‑1 (MQ=255)
                                       gATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACTAATTTTTTTATGTTATTATTTATAAATAATTTATTATACATATTAACCTTTTTAGTGTTTAATTATTACTTATTATTTCGAAAAAc                                                                                                                  <  1:480259/250‑1 (MQ=255)
                                                                 ttttttAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACTAATTTTTTTATGTTATTATTTATAAATAATTTATTATACATATTAACCTTTTTAGTGTTTAATTATTACTTATTATTTCGAAAAACAATATATCTGGAATATaa                                                                                                <  1:560144/242‑1 (MQ=255)
                                                                                    atTGATGATAGCGCATAAGATCATCAGTATCGCTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACTAATTTTTTTATGTTATTATTTATAAATAATTTATTATACATATTAACCTTTTTAGTGTTTAATTATTACTTATTATTTCGAAAAACAATATATCTGGAATATAAAAAACCTAGGATTAGGCTTATACCGGaa                                                                    >  1:842868/1‑251 (MQ=255)
                                                                                                                                                  ttCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACTAATTTTTTTATGTTATTATTTATAAATAATTTATTATACATATTAACCTTTTTAGTGTTTAATTATTACTTATTATTTCGAAAAACAATATATCTGGAATATAAAAAACCTAGGATTAGGCTTATACCGGAAGAGGTTATTAGCGTAATTGCCGGGAGCGCTTCTATTTTATCGGCTATCAATCCTATGAGAt       <  1:763812/250‑1 (MQ=255)
                                                                                                                                                           aTTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACTAATTTTTTTATGTTATTATTTATAAATAATTTATTATACATATTAACCTTTTTAGTGTTTAATTATTAc                                                                                                                                   <  1:58311/117‑1 (MQ=255)
                                                                                                                                                                   aGTGGCACATAATATCCAAATAATACAAGCGATATAAACTAATTTTTTTATGTTATTATTTATAAATAATTTATTATACATATTAACCTTTTTAGTGTTTAATTATTACTTATTATTTCGAAAAACAATATATCTGGAATATAAAAAACCTAGGATTAGGCTTATACCGGAAGAGGTTATTAGCGTAATTGCCGGGAGCGCTTCTATTTTATCGGCTATCAATCCTATGAGATAACTc  >  1:5869/1‑238 (MQ=255)
                                                                                                                                                                                                                        |                                                                                                                                                                                        
ACCAGTGCATGATAACGCCGTCTTGTGGGTTAAATTGAGGATCCGGCAGTAAATACCAATTCCCATTTTTTAACCACTCACTATATTGATGATAGCGCATAAGATCATCAGTATCACTCAATGCGAAATTTGCGACATCACTCCAGTTCCGTATAATTAAAAAAGTGGCACATAATATCCAAATAATACAAGCGATATAAACTAATTTTTTTATGTCATTATTTATAAATAATTTATTATACATATTAACCTTTTTAGTGTTTAATTATTACTTATTATTTCGAAAAACAATATATCTGGAATATAAAAAACCTAGGATTAGGCTTATACCGGAAGAGGTTATTAGCGTAATTGCCGGGAGCGCTTCTATTTTATCGGCTATCAATCCTATGAGATAACTC  >  1/692251‑692651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑