Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 690,971 A→G H508H (CAT→CAC)  unknown ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1690,9710AG100.0% 64.7 / NA 24H508H (CAT→CAC) unknownhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/13);  total (11/13)

AGATAATCAGGTATATCTTTCTTATCTATTAATTTTTGAGCCATGATTTCTTTCCCCTTATATTTATCGGCTAAAACATTGGCAAAATTTTTCTCAATCAGAATATAATCCACATTATTTCTAATCAAGTATTTTTCTGCATCATCTGTATTAACTGACGTATAAAACCGCATTGTATCAGAATTTCCTCTTATATTTCTATGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGTAATATAATTTTATTTTCTAAATTGTTTTTATTAAGTAATTTTTTCGTCGCATAGTAGCTATTATTATATTTTTCGTCATAAATCTCTTTGGATTCATTTTCATCGTTGAAATTTATGATTATTTTAGTTGAAATTAATAGAAATGTAGGTATTGATAAAAATAAAATGAT  >  1/690771‑691210
                                                                                                                                                                                                        |                                                                                                                                                                                                                                               
aGATAATCAGGTATATCTTTCTTATCTATTAATTTTTGAGCCATGATTTCTTTCCCCTTATATTTATCGGCTAAAACATTGGCAAAATTTTTCTCAATCAGAATATAATCCACATTATTTTTAATCAAGTATTTTTCTGCATCATCTGTATTAACTGACGTATAAAACCGCATTGTATCAGAATTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGgcag                                                                                                                                                                                                >  1:99260/1‑250 (MQ=255)
         ggTATATCTTTCTTATCTATTAATTTTTGAGCCATGATTTCTTTCCCCTTATATTTATCGGCTAAAACATTGGCAAAATTTTTCTCAATCAGAATATAATCCACATTATTTTTAATCAAGTATTTTTCTGCATCATCTGTATTAACTGACGTATAAAACCGCATTGTATCAGAATTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGgc                                                                                                                                                                                                  >  1:546466/1‑239 (MQ=255)
             tatCTTTCTTATCTATTAATTTTTGAGCCATGATTTCTTTCCCCTTATATTTATCGGCTAAAACATTGGCAAAATTTTTCTCAATCAGAATATAATCCACATTATTTTTAATCAAGTATTTTTCTGCATCATCTGTATTAACTGACGTATAAAACCGCATTGTATCAGAATTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGTATTGGATGTTGTAGCAATAACGGCAGCACCTAAATCAATa                                                                                                                                                                                  <  1:273666/251‑1 (MQ=255)
                                                  tttcCCCTTATATTTATCGGCTAAAACATTGGCAAAATTTTTCTCAATCAGAATATAATCCACATTATTTTTAATCAAGTAGTTTTCTGCATCATCTGTATTAACTGACGTATAAAACCGCATTGTATCAGAATTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGTAATATAATTTTATTTTCTAAATTGTTTttat                                                                                                                                             <  1:439739/251‑1 (MQ=255)
                                                      cccTTATATTTATCGGCTAAAACATTGGCAAAATTTTTCTCAATCAGAATATAATCCACATTATTTTTAATCAAGTATTTTTCTGCATCATCTGTATTAACTGACGTATAAAACCGCATTGTATCAGAATTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGt                                                                                                                                                                            >  1:556961/1‑216 (MQ=255)
                                                      cccTTATATTTATCGGCTAAAACATTGGCAAAATTTTTCTCAATCAGAATATAATCCACATTATTTTTAATCAAGTATTTTTCTGCATCATCTGTATTAACTGACGTATAAAACCGCATTGTATCAGAATTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGTAatat                                                                                                                                                                       >  1:139466/1‑221 (MQ=255)
                                                                                                 tCAGAATATAATCCACATTATTTTTAATCAAGTATTTTTCTGCATCATCTGTATTAACTGACGTATAAAACCGCATTGTATCAGAATTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAAc                                                                                                                                                                                                     <  1:139765/148‑1 (MQ=255)
                                                                                                        ataATCCACATTATTTTTAATCAAGTATTTTTCTGCATCATCTGTATTAACTGACGTATAAAACCGCATTGTATCAGAATTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGTAATATAATTTTATTTTCTAAATTGTTTTTATTAAGTAATTTTTCCGTCGCATAGTAGCTATTATTATATTTTTCGTCATAAAtct                                                                                       <  1:527916/251‑1 (MQ=255)
                                                                                                                    attTTTAATCAAGTATTTTTCTGCATCATCTGTATTAACTGACGTATAAAACCGCATTGTATCAGAATTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGc                                                                                                                                                                                                                          <  1:721180/108‑1 (MQ=255)
                                                                                                                               aGTATTTTTCTGCATCATCTGTATTAACTGACGTATAAAACCGCATTGTATCAGAATTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGAtgt                                                                                                                                                                                                                 <  1:197866/106‑1 (MQ=255)
                                                                                                                                               atcTGTATTAACTGACGTATAAAACCGCATTGTATCAGAATTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGAt                                                                                                                                                                                                                   <  1:799875/88‑1 (MQ=255)
                                                                                                                                                   gTATTAACTGACGTATAAAACCGCATTGTATCAGAATTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGAtgttgt                                                                                                                                                                                                              <  1:217194/89‑1 (MQ=255)
                                                                                                                                                   gTATTAACTGACGTATAAAACCGCATTGTATCAGAATTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATa                                                                                                                                                                                                       <  1:790473/96‑1 (MQ=255)
                                                                                                                                                   gTATTAACTGACGTATAAAACCGCATTGTATCAGAATTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGTAATATAATTTTATTTTCTAAATTGTTTTTATTAAGTAATTTTTCCGTCGCATAGTAGCTATTATTATATTTTTCGTCATAAATCTCTTTGGATTCATTTTCATCGTTGAAATTTATGATTATTTTAGt                                            >  1:197881/1‑251 (MQ=255)
                                                                                                                                                   gTATTAACTGACGTATAAAACCGCATT‑TATCAGAATTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGTAATATAATTTTATTTTCTAAATTGTTTTTATTAAGTAATTTTTCCGTCGCATAGTAGCTATTATTATATTTTTCGTCATAAATCTCTTTGGATTCATTTTCATCGTTGAAATTTATg                                                       <  1:336391/239‑1 (MQ=255)
                                                                                                                                                             aCGTATAAAACCGCATTGTATCAGAATTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGTAATATAATTTTATTTTCTAAATTGTTTTTATTAAGTAATTTTTCCGTCGCATAGTAGCTATTATTATATTTTTCGTCATAAATCTCTTTGGATTCATTTTCATCGTTGAAATTTATGATTATTTTAGTTGAAATTAAt                                  <  1:336669/251‑1 (MQ=255)
                                                                                                                                                               gTATAAAACCGCATTGTATCAGATTTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGGCAGCACCTAAAt                                                                                                                                                                                       >  1:230539/1‑100 (MQ=255)
                                                                                                                                                               gTATAAAACCGCATTGTATCAGAATTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGGCAGCACCTAAATCAATagg                                                                                                                                                                                >  1:305872/1‑107 (MQ=255)
                                                                                                                                                                 ataAAACCGCATTGTATCAGAATTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGTAATATAattt                                                                                                                                                                  <  1:660317/119‑1 (MQ=255)
                                                                                                                                                                              gTATCAGAATTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGTAATATAat                                                                                                                                                                    >  1:751958/1‑104 (MQ=255)
                                                                                                                                                                              gTATCAGAATTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGTAATATAATTTTATTTTCTAAATTGTTTTTATTAAGTAATTTTTCCGTCGCATAGTAGCTATTATTATATTTTTCGTCATAAATCTCTTTGGATTCATTTTCATCGTTGAAATTTATGATTATTTTAGTTGAAATTAATAGAAATGTAGGTATTGa                 >  1:438127/1‑251 (MQ=255)
                                                                                                                                                                                    gAATTTCCTCTTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGTAATATAATTTTATTTTCTAAATTGTTTTTATTAAGTAATTTTTCCGTCGCATAGTAGc                                                                                                                  >  1:772459/1‑148 (MQ=255)
                                                                                                                                                                                           ctctTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGTAATATAATTTTATTTTCTAAATTGTTTTTATTAAGTAATTTTTCCGTCGCATAGTAGCTATTATTATATTTTTCGTCATAAATCTCTTTGGATTCATTTTCATCGTTGAAATTTAt                                                        <  1:446569/199‑1 (MQ=255)
                                                                                                                                                                                             ctTATATTTCTGTGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGTAATATAATTTTATTTTCTAAATTGTTTTTATTAAGTAATTTTTCCGTCGCATAGTAGCTATTATTATATTTTTCGTCATAAATCTCTTTGGATTCATTTTCATCGTTGAAATTTATGATTATTTTAGTTGAAATTAATAGAAATGTAGGTATTGATAAAAATAAAATGAt  >  1:16505/1‑251 (MQ=255)
                                                                                                                                                                                                        |                                                                                                                                                                                                                                               
AGATAATCAGGTATATCTTTCTTATCTATTAATTTTTGAGCCATGATTTCTTTCCCCTTATATTTATCGGCTAAAACATTGGCAAAATTTTTCTCAATCAGAATATAATCCACATTATTTCTAATCAAGTATTTTTCTGCATCATCTGTATTAACTGACGTATAAAACCGCATTGTATCAGAATTTCCTCTTATATTTCTATGGTAAGGCGCAGAAATAGAAGCATTGGATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGTAATATAATTTTATTTTCTAAATTGTTTTTATTAAGTAATTTTTTCGTCGCATAGTAGCTATTATTATATTTTTCGTCATAAATCTCTTTGGATTCATTTTCATCGTTGAAATTTATGATTATTTTAGTTGAAATTAATAGAAATGTAGGTATTGATAAAAATAAAATGAT  >  1/690771‑691210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑