Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	690,130	C→T	R139H (CGT→CAT)	unknown ←	tRNA threonylcarbamoyladenosine biosynthesis protein TsaE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	690,130	0	C	T	100.0%	94.5 / NA	36	R139H (CGT→CAT)	unknown	tRNA threonylcarbamoyladenosine biosynthesis protein TsaE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/19);  total (17/19)

TTTTTTTCATAAATTTTTAGGTTGCGCCCGATAGCGCCGGGGTCTTTCCCGCCATGACCGGGATCAACGGCAATTGTCCATGCGTTACCGGCGAGAACGGGCATTGCAAAGAAAAAAACGCTGTTGACAAGAAATAATTTAAAGATATTGCCGAGTTTCATTGTGGTACATCATCGTTATTTAATTGTTCAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGACGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCTTTCTCAGCCCACTCAATCAAGCAAAGCGTGTTTTCACTAAAATAATCACGAATGCCCATAAATTCCAGCTCTTCAGGATCGGCAAGACGATACAAATCAAAATGGTAAACGGTTTTGTTGCGGAATCGATATTCTTCCACTAACGTGTAAGTCGGGCTTTTTACTTTACCTTGATACCCCAAAG  >  1/689894‑690376                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                       tttttttCATAATTTTTTAGGTTGCGCCCGATAGCGCCGGGGTCTTTCCCGCCATGACCGGGATCAACGGCAATTGTCCATGCGTTACCGGCGAGAACGGGCATTGCAAAGAAAAAAACGCTGTTGACAAGAAATAATTTAAAGATATTGCCGAGTTTCATTGTGGTACATCATCGTTATTTAATTGTTCAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCa                                                                                                                                                                                                                                          <  1:739727/251‑1 (MQ=255)              tttttAGGTTGCGCCCGATAGCGCCGGGGTCTTTCCCGCCATGACCGGGATCAACGGCAATTGTCCATGCGTTACCGGCGAGAACGGGCATTGCAAAGAAAAAAACGCTGTTGACAAGAAATAATTTAAAGATATTGCCGAGTTTCATTGTGGTACATCATCGTTATTTAATTGTTCAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCATAGGCAATGt                                                                                                                                                                                                                                <  1:592195/248‑1 (MQ=255)                         cgcCCGATAGCGCCGGGGTCTTTCCCGCCATGACCGGGATCAACGGCAATTGTCCATGCGTTACCGGCGAGAACGGGCATTGCAAAGAAAAAAACGCTGTTGACAAGAAATAATTTAAAGATATTGCCGAGTTTCATTGTGGTACATCATCGTTATTTAATTGTTCAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATc                                                                                                                                                                                                                   <  1:555339/250‑1 (MQ=255)                           cccGATAGCGCCGGGGTCTTTCCCGCCATGACCGGGATCAACGGCAATTGTCCATGCGTTACCGGCGAGAACGGGCATTGCAAAGAAAAAAACGCTGTTGACAAGAAATAATTTAAAGATATTGCCGAGTTTCATTGTGGTACATCATCGTTATTTAATTGTTCAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTg                                                                                                                                                                                                                 >  1:678788/1‑250 (MQ=255)                                                   gCCATGACCGGGATCAACGGCAATTGTCCATGCGTTACCGGCGAGAACGGGCATTGCAAAGAAAAAAACGCTGTTGACAAGAAATAATTTAAAGATATTGCCGAGTTTCATTGTGGTACATCATCGTTATTTAATTGTTCAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCttt                                                                                                                                                                                        >  1:393368/1‑251 (MQ=255)                                                        gACCGGGATCAACGGCAATTGTCCATGCGTTACCGGCGAGAACGGGCATTGCAAAGAAAAAAACGCTGTTGACAAGAAATAATTTAAAGATATTGCCGAGTTTCATTGTGGTACATCATCGTTATTTAATTGTTCAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCTTTCTCAg                                                                                                                                                                                   >  1:633483/1‑251 (MQ=255)                                                        gACCGGGATCAACGGCAATTGTCCATGCGTTACCGGCGAGAACGGGCATTGCAAAGAAAAAAACGCTGTTGACAAGAAATAATTTAAAGATATTGCCGAGTTTCATTGTGGTACATCATCGTTATTTAATTGTTCAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCTTTCTCAg                                                                                                                                                                                   >  1:571893/1‑251 (MQ=255)                                                                    cGGCAATTGTCCATGCGTTACCGGCGAGAACGGGCATTGCAAAGAAAAAAACGCTGTTGACAAGAAATAATTTAAAGATATTGCCGAGTTTCATTGTGGTACATCATCGTTATTTAATTGTTCAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCa                                                                                                                                                                                                                                          >  1:722313/1‑184 (MQ=255)                                                                         aTTGTCCATGCGTTACCGGCGAGAACGGGCATTGCAAAGAAAAAAACGCTGTTGACAAGAAATAATTTAAAGATATTGCCGAGTTTCATTGTGGTACATCATCGTTATTTAATTGTTCAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCTTTCTCAGCCCACTCAATCAAGCaa                                                                                                                                                                  <  1:224288/251‑1 (MQ=255)                                                                         aTTGTCCATGCGTTACCGGCGAGAACGGGCATTGCAAAGAAAAAAACGCTGTTGACAAGAAATAATTTAAAGATATTGCCGAGTTTCATTGTGGTACATCATCGTTATTTAATTGTTCAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCTTTCTCAGCCCACTCAATCAAGCaa                                                                                                                                                                  <  1:384555/251‑1 (MQ=255)                                                                            gTCCATGCGTTACCGGCGAGAACGGGCATTGCAAAGAAAAAAACGCTGTTGACAAGAAATAATTTAAAGATATTGCCGAGTTTCATTGTGGTACATCATCGTTATTTAATTGTTCAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCATAGGCAATGTTGAcc                                                                                                                                                                                                                           >  1:225649/1‑191 (MQ=255)                                                                                  gCGTTACCGGCGAGAACGGGCATTGCAAAGAAAAAAACGCTGTTGACAAGAAATAATTTAAAGATATTGCCGAGTTTCATTGTGGTACATCATCGTTATTTAATTGTTCAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTc                                                                                                                                                                                            <  1:467083/216‑1 (MQ=255)                                                                                     ttACCGGCGAGAACGGGCATTGCAAAGAAAAAAACGCTGTTGACAAGAAATAATTTAAAGATATTGCCGAGTTTCATTGTGGTACATCATCGTTATTTAATTGTTCAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCTTTCTCAGCCCACtcaat                                                                                                                                                                         <  1:655243/232‑1 (MQ=255)                                                                                              agaACGGGCATTGCAAAGAAAAAAACGCTGTTGACAAGAAATAATTTAAAGATATTGCCGAGTTTCATTGTGGTACATCATCGTTATTTAATTGTTCAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCTTTCTCAGCCCACTCAATCAAGCaa                                                                                                                                                                  <  1:149693/230‑1 (MQ=255)                                                                                                aaCGGGCATTGCAAAGAAAAAAACGCTGTTGACAAGAAATAATTTAAAGATATTGCCGAGTTTCATTGTGGTACATCATCGTTATTTAATTGTTCAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCTTTCTCAGCCCACTCAATCAAGCAAAGCGTGTTTTCACTAAaataa                                                                                                                                             <  1:542601/249‑1 (MQ=255)                                                                                                               gAAAAAAACGCTGTTGACAAGAAATAATTTAAAGATATTGCCGAGTTTCATTGTGGTACATCATCGTTATTTAATTGTTCAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCTTTCTCAGCCCACTCAATCAAGc                                                                                                                                                                    <  1:101324/211‑1 (MQ=255)                                                                                                                aaaaaaaCGCTGTTGACAAGAAATAATTTAAAGATATTGCCGAGTTTCATTGTGGTACATCATCGTTATTTAATTGTTCAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCTTTCTCAGCCCACTCAATCAAGCAAAGCGTGTTTTCACTAAAATAATCACGAATGCCCATaaa                                                                                                                            <  1:739086/250‑1 (MQ=255)                                                                                                                         cTGTTGACAAGAAATAATTTAAAGATATTGCCGAGTTTCATTGTGGTACATCATCGTTATTTAATTGTTCAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCTTtctc                                                                                                                                                                                     >  1:849383/1‑184 (MQ=255)                                                                                                                                                           gTTTCATTGTGGTACATCATCGTTATTTAATTGTTCAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCTTTCTCAGCCCACTCAATCAAGCAAAGCGTGTTTTCACTAAAATAATCACGAATGCCCATAAATTCCAGCTCTTCAGGATCGGCAAGACGATACAAATCAAAATGGt                                                                                >  1:311983/1‑251 (MQ=255)                                                                                                                                                                   gtgGTACATCATCGTTATTTAATTGTTCAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCTTTCTCAGCCCACTCAATCAAGCAAAGCGTGTTTTCACTAAAATAATCACGAATGCCCATAAATTc                                                                                                                         >  1:372953/1‑202 (MQ=255)                                                                                                                                                                   gtgGTACATCATCGTTATTTAATTGTTCAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCTTTCTCAGCCCACTCAATCAAGCAAAGCGTGTTTTCACTAAAATAATCACGAATGCCCATAAATTCCAGCTCTTCAGGAt                                                                                                           <  1:646627/216‑1 (MQ=255)                                                                                                                                                                       tACATCATCGTTATTTAATTGTTCAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCTTTCTCAGCCCACTCAATCAAGCAAAGCGTGTTTTCACTAAAATAATCACGAATGCCCATAAATTCCAGCTCTTCAGGATCGGCAAGACGATACAAATCAAAATGGTAAACGGTTttgt                                                                    <  1:307163/251‑1 (MQ=255)                                                                                                                                                                             atcGTTATTTAATTGTTCAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCTTTCTCAGCCCACTCAATCAAGCAAAGCGTGTTTTCACTAAAATAATCACGAATGCCCATAAATTCCAGCTCTTCAGGATCGGCAAGACGATACAAATCAAAATGGTAAACGGTTTTGTTGCGGa                                                              >  1:893438/1‑251 (MQ=255)                                                                                                                                                                                 ttATTTAATTGTTCAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCTTTCTCAGCCCACTCAATCAAGCAAAGCGTGTTTTCACTAAAATAATCACGAATGCCCATAAATTCCAGCTCTTCAGGATCGGCAAGACGATACAAATCAAAATGGTAAACgg                                                                          >  1:308945/1‑235 (MQ=255)                                                                                                                                                                                      tAATTGTTCAATAATCGTTCTGCCGGTTGGCAATTGAGCAACCAATTCAATGTGATGGGGAGTTTCCGCATAGGCAATGTTGACCAACAAAACTGCCGCCATAAGCATGCCTGGTCCTTTTTCAGGCCACTTAATCAAGCAAAGCGTGTTTTTACTAAAATTATCACGAAATCCCATAAATTCCAGCTCTTCAGGATCGGCAAGACGATACAAATCAAAATGGTAAACGGTTTTGTTGCGGAATCGATAtt                                                     <  1:900895/251‑1 (MQ=255)                                                                                                                                                                                           gTTCAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCTTTCTCAGCCCACTCAATCAAGCAAAGCGTGTTTTCACTAAAATAATCACGAATGCCCATAAATTCCAGCTCTTCAGGATCGGCAAGACGATACAAATCAAAATGGTAAACGGTTTTGTTGCGGAATCGATAttcttc                                                 >  1:160369/1‑250 (MQ=255)                                                                                                                                                                                            ttCAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCTTTCTCAGCCCACTCAATCAAGCAAAGCGTGTTTTCACTAAAATAATCACGAATGCCCATAAATTCCAGCTCTTCAGGATCGGCAAGACGATACAAATCAAAATGGTAAACGGTTTTGTTGCGGAATCGATATTCTTcc                                                <  1:689330/250‑1 (MQ=255)                                                                                                                                                                                              cAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCTTTCTCAGCCCACTCAATCAAGc                                                                                                                                                                    >  1:570193/1‑132 (MQ=255)                                                                                                                                                                                              cAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCTTTCTCAGCCCACTCAATCAAGCaa                                                                                                                                                                  <  1:425498/134‑1 (MQ=255)                                                                                                                                                                                                ataatCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCTTTCTCAGCCCACTCAATCAAGCAAAGCGTGTTTTCACTAAAATAATCACGAATGCCCATAAATTCCAGCTCTTCAGGATCGGCAAGACGATACAAATCAAAATGGTAAACGGTTTTGTTGCGGAATCGATATTCTTCCACTaa                                           <  1:329804/251‑1 (MQ=255)                                                                                                                                                                                                       gtctgCCGATTGGCGATTGAGCAACCAATTCAATGTGATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCTTTCTCAGCCCACTCAATCAAGCAAAGCGTGTTTTCACTAAAATAATCACGAATGCCCATAAATTCCAGCTCTTCAGGATCGGCAAGACGATACAAATCAAAATGGTAAACGGTTTTGTTGCGGAATCGATATTCTTCCACTAACGTGTaa                                    >  1:586922/1‑251 (MQ=255)                                                                                                                                                                                                                         gAGCAACCAATTCAATGTGATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCTTTCTCAGCCCACTCAATCAAGCAAAGCGTGTTTTCACTAAAATAATCACGAATGCCCATAAATTCCAGCTCTTCAGGATCGGCAAGACGATACAAATCAAAATGGTAAACGGTTTTGTTGCGGAATCGATATTCTTCCACTAACGTGTAAGt                                  >  1:676121/1‑235 (MQ=255)                                                                                                                                                                                                                                 aaTTCAATGTGATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCTTTCTCAGCCCACTCAATCAAGCAAAGCGTGTTTTCACTAAAATAATCACGAATGCCCATAAATTCCAGCTCTTCAGGAt                                                                                                           >  1:591865/1‑154 (MQ=255)                                                                                                                                                                                                                                  attacaTGTGATGGGCAGTTACCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCTTTCTCAGCCCACTCAATCAAGCAAAGCGTGTTTTCACTAAAATAATCACGAATGCCCATAAATTCCAGCTCTTCAGGATCGGCAAGACGATACAAATCAAAATGGTAAACGGTTTTGTTGCGGAATCGATATTCTTCCACTACCGTGTAAGt                                  <  1:292160/221‑1 (MQ=255)                                                                                                                                                                                                                                         gtgATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCTTTCTCAGCCCACTCAATCAAGCAAAGCGTGTTTTCACTAAAATAATCACGAATGCCCATAAATTCCAGCTCTTCAGGAt                                                                                                           <  1:290513/146‑1 (MQ=255)                                                                                                                                                                                                                                         gtgATGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCTTTCTCAGCCCACTCAATCAAGCAAAGCGTGTTTTCACTAAAATAATCACGAATGCCCATAAATTCCAGCTCTTCAGGATCGGCAAGACGATACAAATCAAAATGGTAAACGGTTTTGTTGCGGAATCGATATTCTTCCACTAACGTGTAAGTCGGGCTTTTTACTTTACCTTGATACCCCAAAg  >  1:67017/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                       TTTTTTTCATAAATTTTTAGGTTGCGCCCGATAGCGCCGGGGTCTTTCCCGCCATGACCGGGATCAACGGCAATTGTCCATGCGTTACCGGCGAGAACGGGCATTGCAAAGAAAAAAACGCTGTTGACAAGAAATAATTTAAAGATATTGCCGAGTTTCATTGTGGTACATCATCGTTATTTAATTGTTCAATAATCTGTCTGCCGATTGGCGATTGAGCAACCAATTCAATGTGACGGGCAGTTTCCGCATAGGCAATGTTGACCAACAAATCTGCCGCCATAAGCATGCCTGTTCCTTTCTCAGCCCACTCAATCAAGCAAAGCGTGTTTTCACTAAAATAATCACGAATGCCCATAAATTCCAGCTCTTCAGGATCGGCAAGACGATACAAATCAAAATGGTAAACGGTTTTGTTGCGGAATCGATATTCTTCCACTAACGTGTAAGTCGGGCTTTTTACTTTACCTTGATACCCCAAAG  >  1/689894‑690376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑