Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	687,656	T→C	P304P (CCA→CCG)	unknown ←	DNA mismatch repair protein MutL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	687,656	0	T	C	100.0%	160.9 / NA	58	P304P (CCA→CCG)	unknown	DNA mismatch repair protein MutL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/31);  total (27/31)

TCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTCCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTTGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATGGTTTTATCACGCACCATGCGCCCGTTGACATAGCAATAATTCAGGTCGTTTTGCGGACGGTTAAACTGCGGCAACGCCACCCAGCCGGATAAATGCAGGTCATCGTGTTTCCAGT  >  1/687412‑687885                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                      tCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGc                                                                                                                                                                                                                                 <  1:278317/251‑1 (MQ=255)       tttCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTc                                                                                                                                                                                                                           <  1:646442/251‑1 (MQ=255)           ggCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACAt                                                                                                                                                                                                                       >  1:574963/1‑251 (MQ=255)            gCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGcac                                                                                                                                                                                                                               >  1:138499/1‑242 (MQ=255)            gCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACAt                                                                                                                                                                                                                       <  1:129597/250‑1 (MQ=255)             cGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATcc                                                                                                                                                                                                                     <  1:687346/251‑1 (MQ=255)             cGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATcc                                                                                                                                                                                                                     <  1:88721/251‑1 (MQ=255)                      tGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTc                                                                                                                                                                                                            <  1:325928/251‑1 (MQ=255)                           gAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATcc                                                                                                                                                                                                                     >  1:442787/1‑237 (MQ=255)                                 ccGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGAt                                                                                                                                                                                                  >  1:811024/1‑250 (MQ=255)                                        tCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATaa                                                                                                                                                                                            <  1:168800/249‑1 (MQ=255)                                           gTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAaacaa                                                                                                                                                                                        >  1:799222/1‑250 (MQ=255)                                              cGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATaa                                                                                                                                                                                            <  1:597342/243‑1 (MQ=255)                                                aaTATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTTTTCTACGGCATTTTCCATTGATGACGTGGTCAAGTGTATTTGCTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACaaa                                                                                                                                                                                  >  1:607227/1‑251 (MQ=255)                                                aaTATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACaaa                                                                                                                                                                                  >  1:408079/1‑251 (MQ=255)                                                     atTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATcc                                                                                                                                                                                                                     >  1:679809/1‑211 (MQ=255)                                                           cccTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGatat                                                                                                                                                                        <  1:132206/250‑1 (MQ=255)                                                                ccgccgAGCGATTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCa                                                                                                                                                                  >  1:482566/1‑251 (MQ=255)                                                                          aTTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGatat                                                                                                                                                                        >  1:522129/1‑235 (MQ=255)                                                                          aTTCGGTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTaaa                                                                                                                                                         <  1:809214/250‑1 (MQ=255)                                                                               gTTTGGTGTAAGTCGGCGCTTTCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAaac                                                                                                                                                                                          <  1:315398/212‑1 (MQ=255)                                                                                                 ctttccACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATc                                                                                                                                 <  1:475269/247‑1 (MQ=255)                                                                                                   ttccACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATcg                                                                                                                                >  1:574901/3‑250 (MQ=255)                                                                                                    tccACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATcgcg                                                                                                                              >  1:810876/2‑251 (MQ=255)                                                                                                          cacCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTaaa                                                                                                                                                         <  1:604255/218‑1 (MQ=255)                                                                                                              cGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATgg                                                                                                                    <  1:377342/251‑1 (MQ=255)                                                                                                              cGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATgg                                                                                                                    <  1:34209/251‑1 (MQ=255)                                                                                                                           aCGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATGGTTTTATCACGCAc                                                                                                       <  1:168013/251‑1 (MQ=255)                                                                                                                           aCGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATGGTTTTATCACGCAc                                                                                                       <  1:871945/251‑1 (MQ=255)                                                                                                                              gCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATGGTTTTATCACGCACCAt                                                                                                    >  1:484305/1‑251 (MQ=255)                                                                                                                                           atgaCGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATGGTTTTATCACGCACCATGCGCCCGTTGAc                                                                                        <  1:494942/250‑1 (MQ=255)                                                                                                                                                  ggTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGt                                                                                                                                                                     <  1:490152/166‑1 (MQ=255)                                                                                                                                                  ggTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATGGTTTTATCACGCACCATGCGCCCGTTGACATAGCaat                                                                                >  1:269299/1‑251 (MQ=255)                                                                                                                                                       aGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATGGTTTTATCACGCACCATGCGCCCGTTGACATAGCAATAAtt                                                                            >  1:50484/1‑250 (MQ=255)                                                                                                                                                        gtgtATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATACACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATGGTTTTATCACGCACCATGCGCCCGTTGACATAGCAATAATTCa                                                                          >  1:900764/1‑251 (MQ=255)                                                                                                                                                        gtgtATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATGGTTTTATCACGCACCATGCGCCCGTTGACATAGCAATAATTCa                                                                          >  1:666910/1‑251 (MQ=255)                                                                                                                                                          gtATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGt                                                                                                                                                                     >  1:777621/1‑158 (MQ=255)                                                                                                                                                           tATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCAt                                                                                                                                               >  1:192022/1‑179 (MQ=255)                                                                                                                                                           tATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATGGTTTTATCACGCACCATGCGCCCGTTGACATAGCAATAATTCAGGt                                                                       <  1:175212/251‑1 (MQ=255)                                                                                                                                                                gTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATcc                                                                                                                                                                                                                     <  1:167896/104‑1 (MQ=255)                                                                                                                                                                  tCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATGGTTTTATCACGCACCATGCGCCCGTTGACATAGCAATAATTCAGGTCGTTTTg                                                                >  1:31035/1‑251 (MQ=255)                                                                                                                                                                               cgTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATGGTTTTATCACGCACCATGCGCCCGTTGACATAGCAATAATTCAGGt                                                                       >  1:658025/1‑231 (MQ=255)                                                                                                                                                                               cgTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATGGTTTTATCACGCACCATGCGCCCGTTGACATAGCAATAATTCAGGTCGTTTTGCGGACGGTTAAAc                                                   <  1:363951/251‑1 (MQ=255)                                                                                                                                                                                   aCTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATGGTTTTATCACGCACCATGCGCCCGTTGAc                                                                                        <  1:876409/210‑1 (MQ=255)                                                                                                                                                                                   aCTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATGGTTTTATCACGCACCATGCGCCCGTTGACATAGCAATAATTCAGGTCGTTTTGCGGACGGTTAAACTGCg                                               >  1:406494/1‑251 (MQ=255)                                                                                                                                                                                                        aTCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATGGTTTTATCACGCACCATGCGcc                                                                                               >  1:144858/1‑182 (MQ=255)                                                                                                                                                                                                        aTCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATGGTTTTATCACGCACCATGCGCCCGTTGACATAGCAATAATTCAGGTCGTTTTGCGGACGGTTAAACTGCGGCAACGCCAccc                                   >  1:33840/1‑242 (MQ=255)                                                                                                                                                                                                             gCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATGGTTTTATCACGCACCATGCGCCCGTTGAc                                                                                        >  1:433572/1‑184 (MQ=255)                                                                                                                                                                                                              cACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATGGTTTTATCACGCACCATGCGCCCGTTGACATAGCAATAATTCAGGTCGTTTTGCGGACGGTTAAACTGCGGCAACGCCACCCAGCCGGATAAATGc                     <  1:722069/250‑1 (MQ=255)                                                                                                                                                                                                              cACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATGGTTTTATCACGCACCATGCGCCCGTTGACATAGCAATAATTCAGGTCGTTTTGCGGACGGTTAAACTGCGGCAACGCCACCCAGCCGGATAAATGc                     <  1:83630/250‑1 (MQ=255)                                                                                                                                                                                                              cACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATGGTTTTATCACGCACCATGCGCCCGTTGACATAGCAATAATTCAGGTCGTTTTGCGGACGGTTAAACTGCGGCAACGCCACCCAGCCGGATAAATGc                     <  1:881758/250‑1 (MQ=255)                                                                                                                                                                                                                  aaTCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCgg                                                                                                                                    <  1:705120/135‑1 (MQ=255)                                                                                                                                                                                                                  aaTCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATGGTTTTATCACGCACCATGCGCCCGTTGACATAGCAATAATTCAGGTCGTTTTGCGGACGGTTAAACTGCGGCAACGCCACCCAGCCGGATAAATGCAGGt                 >  1:619925/1‑250 (MQ=255)                                                                                                                                                                                                                  aaTCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATGGTTTTATCACGCACCATGCGCCCGTTGACATAGCAATAATTCAGGTCGTTTTGCGGACGGTTAAACTGCGGCAACGCCACCCAGCCGGATAAATGCAGGTc                <  1:297447/251‑1 (MQ=255)                                                                                                                                                                                                                  aaTCGGGATTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATGGTTTTATCACGCACCATGCGCCCGTTGACATAGCAATAATTCAGGTCGTTTTGCGGACGGTTAAACTGCGGCAACGCCACCCAGCCGGATAAATGCAGGTc                <  1:703707/251‑1 (MQ=255)                                                                                                                                                                                                                         aTTGCTGGAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATGGTTTTATCACGCACCATGCGCCCGTTGACATAGCAATAATTCAGGTCGTTTTGCGGACGGTTAAACTGCGGCAACGCCACCCAGCCGGATAAATGCAGGTCATCgtg          <  1:499814/250‑1 (MQ=255)                                                                                                                                                                                                                               ggAAACGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATGGTTTTATCACGCACCATGCGCCCGTTGACATAGCAATAATTCAGGTCGTTTTGCGGACGGTTAAACTGCGGCAACGCCACCCAGCCGGATaa                         >  1:768552/1‑229 (MQ=255)                                                                                                                                                                                                                                 aaaCGCACTTCGTGTTTGGTCGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATGGTTTTATCACGCACCATGCGCCCGTTGACATAGCAATAATTCAGGTCGTTTTGCGGACGGTTAAACTGCGGCAACGCCACCCAGCCGGATAAATGCAGGTCATCGTGTTTCCAGt  <  1:277753/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                      TCTTTTTTTCGGCGTAGGCAGTGGCGGAATAACCGGTAGTCGGTGCGAATATATTTTGCCCTGCCGCCGAGCGATTCGGTTTGGTGTAAGTCGGCGCTTCCCACACACCCGCAGGTTCTTCTACGGCATTTTCCATTGATGACGGGGTCAAGTGTATTTGTTCTGAAGCCAGCGCGTTACTAATGCCTTGGGTGATGAAATCATGCACCAATCGGGATTGCTGGAAACGCACTTCGTGTTTGGTTGGGTGCACATTCACATCCACCTCATTCGGATTGAGATCAATAAACAACACAAAAGCGGGATATTGTTCAGTGGTTAAATAATCCGCATAGGCTTGGCGGATCGCGTGCGTAATGGTTTTATCACGCACCATGCGCCCGTTGACATAGCAATAATTCAGGTCGTTTTGCGGACGGTTAAACTGCGGCAACGCCACCCAGCCGGATAAATGCAGGTCATCGTGTTTCCAGT  >  1/687412‑687885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑