Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	685,896	A→G	G269G (GGT→GGC)	unknown ←	tRNA dimethylallyltransferase (EC 2.5.1.75)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	685,896	0	A	G	100.0%	177.9 / NA	64	G269G (GGT→GGC)	unknown	tRNA dimethylallyltransferase (EC 2.5.1.75)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/23);  total (41/23)

CAATACAGCCAAGCTGTACGGCCTCCGAGATTCAATATAACAACAATTGTTTAGAAAGTCACTAGGCGCAATAGGGTTTTACGCTTTATTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATACCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGC  >  1/685680‑686130                                                                                                                                                                                                                         |                                                                                                                                                                                                                                           cAATACAGCCAAGCTGTACGGCCTCCGAGATTCAATATAACAACAATTGTTTAGAAAGTCACTAGGCGCAATAGGGTTTTACGCTTTATTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGaa                                                                                                                                                                                                                                     >  1:160156/1‑224 (MQ=255) cAATACAGCCAAGCTGTACGGCCTCCGAGATTCAATATAACAACAATTGTTTAGAAAGTCACTAGGCGCAATAGGGTTTTACGCTTTATTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTtcat                                                                                                                                                                                                                         >  1:800487/1‑236 (MQ=255) cAATACAGCCAAGCTGTACGGCCTCCGAGATTCAATATAACAACAATTGTTTAGAAAGTCACTAGGCGCAATAGGGTTTTACGCTTTATTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTca                                                                                                                                                                                                           >  1:293763/1‑250 (MQ=255)      cAGCCAAGCTGTACGGCCTCCGAGATTCAATATAACAACAATTGTTTAGAAAGTCACTAGGCGCAATAGGGTTTTACGCTTTATTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTc                                                                                                                                                                                                            >  1:617298/1‑244 (MQ=255)      cAGCCAAGCTGTACGGCCTCCGAGATTCAATATAACAACAATTGTTTAGAAAGTCACTAGGCGCAATAGGGTTTTACGCTTTATTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACg                                                                                                                                                                                                      >  1:375226/1‑250 (MQ=255)           aaGCTGTACGGCCTCCGAGATTCAATATAACAACAATTGTTTAGAAAGTCACTAGGCGCAATAGGGTTTTACGCTTTATTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTtcatc                                                                                                                                                                                                                        >  1:743028/1‑227 (MQ=255)              cTGTACGGCCTCCGAGATTCAATATAACAACAATTGTTTAGAAAGTCACTAGGCGCAATAGGGTTTTACGCTTTATTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTc                                                                                                                                                                                             <  1:270265/251‑1 (MQ=255)              cTGTACGGCCTCCGAGATTCAATATAACAACAATTGTTTAGAAAGTCACTAGGCGCAATAGGGTTTTACGCTTTATTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGGGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAg                                                                                                                                                                                                              >  1:93287/1‑234 (MQ=255)                         ccGAGATTCAATATAACAACAATTGTTTAGAAAGTCACTAGGCGCAATAGGGTTTTACGCTTTATTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAatat                                                                                                                                                                                               <  1:106870/238‑1 (MQ=255)                         ccGAGATTCAATATAACAACAATTGTTTAGAAAGTCACTAGGCGCAATAGGGTTTTACGCTTTATTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGa                                                                                                                                                                                   >  1:534705/1‑250 (MQ=255)                                 cAATATAACAACAATTGTTTAGAAAGTCACTAGGCGCAATAGGGTTTTACGCTTTATTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACg                                                                                                                                                                                                      <  1:273108/223‑1 (MQ=255)                                   atatAACAACAATTGTTTAGAAAGTCACTAGGCGCAATAGGGTTTTACGCTTTATTTGAAAGCAAACTTAACGCTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGGCACCACGCAAATATTCCCACATTTGACGATACCCcac                                                                                                                                                                        <  1:656878/251‑1 (MQ=255)                                   atatAACAACAATTGTTTAGAAAGTCACTAGGCGCAATAGGGTTTTACGCTTTATTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAAcc                                                                                                                                                                                                                                <  1:868998/195‑1 (MQ=255)                                   atatAACAACAATTGTTTAGAAAGTCACTAGGCGCAATAGGGTTTTACGCTTTATTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTc                                                                                                                                                                                                            >  1:741818/1‑215 (MQ=255)                                   atatAACAACAATTGTTTAGAAAGTCACTAGGCGCAATAGGGTTTTACGCTTTATTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACg                                                                                                                                                                                 >  1:501306/1‑242 (MQ=255)                                     ataACAACAATTGTTTAGAAAGTCACTAGGCGCAATAGGGTTTTACGCTTTATTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCcacac                                                                                                                                                                      <  1:347872/251‑1 (MQ=255)                                      taACAACAATTGTTTAGAAAGTCACTAGGCGCAATAGGGTTTTACGCTTTATTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGGGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTc                                                                                                                                                                                                            >  1:884123/1‑212 (MQ=255)                                                 gTTTAGAAAGTCACTAGGCGCAATAGGGTTTTACGCTTTATTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAgg                                                                                                                                                          >  1:298248/1‑251 (MQ=255)                                                 gTTTAGAAAGTCACTAGGCGCAATAGGGTTTTACGCTTTATTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAgg                                                                                                                                                          >  1:415653/1‑251 (MQ=255)                                                      gAAAGTCACTAGGCGCAATAGGGTTTTACGCTTTATTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAg                                                                                                                                                                                                              >  1:76529/1‑194 (MQ=255)                                                      gAAAGTCACTAGGCGCAATAGGGTTTTACGCTTTATTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTT‑GCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAAt                                                                                                                                                    >  1:671608/1‑251 (MQ=255)                                                         aGTCACTAGGCGCAATAGGGTTTTACGCTTTATTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAAtt                                                                                                                                                   >  1:321763/1‑250 (MQ=255)                                                         aGTCACTAGGCGCAATAGGGTTTTACGCTTTATTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAAtt                                                                                                                                                   >  1:751242/1‑250 (MQ=255)                                                                     cAATAGGGTTTTACGCTTTATTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAatat                                                                                                                                                                                               >  1:531510/1‑194 (MQ=255)                                                                     cAATAGGGTTTTACGCTTTATTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCa                                                                                                                                      >  1:758101/1‑251 (MQ=255)                                                                                 aCGCTTTATTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTAc                                                                                                                          <  1:240718/251‑1 (MQ=255)                                                                                    cTTTATTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGt                                                                                                                                                                                                             <  1:524395/165‑1 (MQ=255)                                                                                      ttATTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGCGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACg                                                                                                                                                                                 >  1:4165/1‑191 (MQ=255)                                                                                        aTTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGttttt                                                                                                                   <  1:553806/251‑1 (MQ=255)                                                                                             aaaGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGAc                                                                                                               <  1:850537/250‑1 (MQ=255)                                                                                             aaaGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGAc                                                                                                               <  1:477111/250‑1 (MQ=255)                                                                                                   aaCTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAg                                                                                                                                                                                                              >  1:372117/1‑149 (MQ=255)                                                                                                   aaCTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGa                                                                                                                                                                 >  1:490456/1‑194 (MQ=255)                                                                                                    aCTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCcacac                                                                                                                                                                      >  1:719174/1‑188 (MQ=255)                                                                                                    aCTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCttg                                                                                                         <  1:209614/249‑1 (MQ=255)                                                                                                     cTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCttgttg                                                                                                      >  1:217818/1‑251 (MQ=255)                                                                                                       tAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGaa                                                                                                    <  1:177037/251‑1 (MQ=255)                                                                                                             tttCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCaa                                                                                              >  1:712749/1‑251 (MQ=255)                                                                                                                        cTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATAccc                                                                                                                                                                           >  1:360553/1‑163 (MQ=255)                                                                                                                                ggTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGaaa                                                                           >  1:499710/1‑251 (MQ=255)                                                                                                                                 gTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGaaaa                                                                          >  1:868458/1‑251 (MQ=255)                                                                                                                                 gTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAACTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATc                                                                                                                                       >  1:688810/1‑190 (MQ=255)                                                                                                                                 gTTGTAAACTGTCCAGCCATTGAATCGGCGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGCGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGaaaa                                                                          >  1:210769/1‑251 (MQ=255)                                                                                                                                         cTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTc                                                                  <  1:773870/251‑1 (MQ=255)                                                                                                                                                gCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATg                                                                              <  1:8758/232‑1 (MQ=255)                                                                                                                                                 ccATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACg                                                                                                                                                                                 >  1:430325/1‑132 (MQ=255)                                                                                                                                                      gAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATg                                                      >  1:477372/1‑250 (MQ=255)                                                                                                                                                            gTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTcac                                                                                                                                                                                                          >  1:313159/1‑96 (MQ=255)                                                                                                                                                            gTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCa                                                                                     >  1:619598/1‑213 (MQ=255)                                                                                                                                                                tttCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGAtctc                                           <  1:762494/251‑1 (MQ=255)                                                                                                                                                                   ccAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATctctct                                        <  1:330834/251‑1 (MQ=255)                                                                                                                                                                       ccGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGAt                                              >  1:492357/1‑241 (MQ=255)                                                                                                                                                                            tAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATAccc                                                                                                                                                                           <  1:620733/111‑1 (MQ=255)                                                                                                                                                                                cAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACg                                                                                                                                                                                 >  1:759112/1‑101 (MQ=255)                                                                                                                                                                                      aTCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTtcat                                                                                                                                                                                                                         >  1:654279/1‑55 (MQ=255)                                                                                                                                                                                      aTCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTc                                                                  <  1:755618/206‑1 (MQ=255)                                                                                                                                                                                      aTCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGaa                     >  1:608535/1‑251 (MQ=255)                                                                                                                                                                                            cGCTTGGCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAg               >  1:737094/1‑251 (MQ=255)                                                                                                                                                                                                  gCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCaa                            <  1:876939/232‑1 (MQ=255)                                                                                                                                                                                                  gCTAATTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAAtct          >  1:902089/1‑250 (MQ=255)                                                                                                                                                                                                     aaTTGGCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATa      >  1:169400/1‑251 (MQ=255)                                                                                                                                                                                                          gCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGc  <  1:711361/250‑1 (MQ=255)                                                                                                                                                                                                          gCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGc  <  1:697904/250‑1 (MQ=255)                                                                                                                                                                                                          gCGGGTGGCGCAGATGCCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGc  <  1:856113/250‑1 (MQ=255)                                                                                                                                                                                                                         |                                                                                                                                                                                                                                           CAATACAGCCAAGCTGTACGGCCTCCGAGATTCAATATAACAACAATTGTTTAGAAAGTCACTAGGCGCAATAGGGTTTTACGCTTTATTTGAAAGCAAACTTAACACTTTCTCAAGCGCTTGAGCCGGTTGTAAACTGTCCAGCCATTGAATCGGTGATTTCCAACCGCGTAACCAAGTAATCTGACGCTTGGCTAATTGGCGGGTGGCGCAGATACCACGAAAAACCATTTCATCATGGCTATAGTCACCACGCAAATATTCCCACATTTGACGATACCCCACACAGCGAATGGAGGGCAAATTCTCGTGCAAATCAGCGCGTCGGTACAGTTTTTCGACCTCTTGTTGAAAGCCAAGTTCAATCATTTTATGAAAACGTTGCTCAATGCGTTGATGCAACAGATCTCTCTGCTCCGGTGCAATGGCGAATTGCAGAATCTCATAGGGC  >  1/685680‑686130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑