Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	684,230	G→A	E76E (GAG→GAA)	unknown →	Transcriptional repressor protein TyrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	684,230	0	G	A	100.0%	196.0 / NA	69	E76E (GAG→GAA)	unknown	Transcriptional repressor protein TyrR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/29);  total (40/29)

TTATTGAGGCACTTATGGCAACTTCTTCAACCACCGATCCTTTTTCACACATCGTCAGCCGAAATCCGCGAATGCGGGATGTTATTGCGAAAGCCAGAAAATTTGCGTTATTGGATGCGCCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAGGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCTTCTGCGTTTTTTAAGCGACGGTACTTTCCGCCGTGTCGGAGAGGAAGAGGAGCATTATGCCAATGTACGTGTGATTTGCACGGCGCAGCAGCCGTTACAGCATTATGT  >  1/683989‑684469                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                ttattGAGGCACTTATGGCAACTTCTTCAACCACCGATCCTTTTTCACACATCGTCAGCCGAAATCCGCGAATGCGGGATGTTATTGCGAAAGCCAGAAAATTTGCGTTATTGGATGCGCCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGcggaa                                                                                                                                                                                                                                        >  1:392587/1‑251 (MQ=255)           aCTTATGGCAACTTCTTCAACCACCGATCCTTTTTCACACATCGTCAGCCGAAATCCGCGAATGCGGGATGTTATTGCGAAAGCCAGAAAATTTGCGTTATTGGATGCGCCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGTAAACGGAAATGt                                                                                                                                                                                                                              >  1:269569/1‑251 (MQ=255)           aCTTATGGCAACTTCTTCAACCACCGATCCTTTTTCACACATCGTCAGCCGAAATCCGCGAATGCGGGATGTTATTGCGAAAGCCAGAAAATTTGCGTTATTGGATGCGCCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGt                                                                                                                                                                                                                              >  1:583935/1‑251 (MQ=255)           aCTTATGGCAACTTCTTCAACCACCGATCCTTTTTCACACATCGTCAGCCGAAATCCGCGAATGCGGGATGTTATTGCGAAAGCCAGAAAATTTGCGTTATTGGATGCGCCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGt                                                                                                                                                                                                                              >  1:615687/1‑251 (MQ=255)            cTTATGGCAACTTCTTCAACCACCGATCCTTTTTCACACATCGTCAGCCGAAATCCGCGAATGCGGGATGTTATTGCGAAAGCCAGAAAATTTGCGTTATTGGATGCGCCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATg                                                                                                                                                                                                                               >  1:342066/1‑249 (MQ=255)            cTTATGGCAACTTCTTCAACCACCGATCCTTTTTCACACATCGTCAGCCGAAATCCGCGAATGCGGGATGTTATTGCGAAAGCCAGAAAATTTGCGTTATTGGATGCGCCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGtt                                                                                                                                                                                                                             >  1:896606/1‑251 (MQ=255)                        tcttcAACCACCGATCCTTTTTCACACATCGTCAGCCGAAATCCGCGAATGCGGGATGTTATTGCGAAAGCCAGAAAATTTGCGTTATTGGATGCGCCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGGCGCGGAAACGGAAATGTTCGGGCGGCGtaa                                                                                                                                                                                                                 >  1:124702/1‑251 (MQ=255)                        tcttcAACCACCGATCCTTTTTCACACATCGTCAGCCGAAATCCGCGAATGCGGGATGTTATTGCGAAAGCCAGAAAATTTGCGTTATTGGATGCGCCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGtaa                                                                                                                                                                                                                 >  1:813547/1‑251 (MQ=255)                           tcAACCACCGATCCTTTTTCACACATCGTCAGCCGAAATCCGCGAATGCGGGATGTTATTGCGAAAGCCAGAAAATTTGCGTTATTGGATGCGCCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGtaataa                                                                                                                                                                                                              <  1:819305/251‑1 (MQ=255)                             aaCCACCGATCCTTTTTCACACATCGTCAGCCGAAATCCGCGAATGCGGGATGTTATTGCGAAAGCCAGAAAATTTGCGTTATTGGATGCGCCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAg                                                                                                                                                                                                            <  1:88704/251‑1 (MQ=255)                             aaCCACCGATCCTTTTTCACACATCGTCAGCCGAAATCCGCGAATGCGGGATGTTATTGCGAAAGCCAGAAAATTTGCGTTATTGGATGCGCCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAg                                                                                                                                                                                                            <  1:816309/251‑1 (MQ=255)                              accaccGATCCTTTTTCACACATCGTCAGCCGAAATCCGCGAATGCGGGATGTTATTGCGAAAGCCAGAAAATTTGCGTTATTGGATGCGCCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGa                                                                                                                                                                                                           <  1:252680/251‑1 (MQ=255)                              accaccGATCCTTTTTCACACATCGTCAGCCGAAATCCGCGAATGCGGGATGTTATTGCGAAAGCCAGAAAATTTGCGTTATTGGATGCGCCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGa                                                                                                                                                                                                           <  1:464153/251‑1 (MQ=255)                                       ccTTTTTCACACATCGTCAGCCGAAATCCGCGAATGCGGGATGTTATTGCGAAAGCCAGAAAATTTGCGTTATTGGATGCGCCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAAtt                                                                                                                                                                                                  >  1:308374/1‑251 (MQ=255)                                            ttCACACATCGTCAGCCGAAATCCGCGAATGCGGGATGTTATTGCGAAAGCCAGAAAATTTGCGTTATTGGATGCGCCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCg                                                                                                                                                                                             >  1:357744/1‑251 (MQ=255)                                                          gCCGAAATCCGCGAATGCGGGATGTTATTGCGAAAGCCAGAAAATTTGCGTTATTGGATGCGCCGTTGGCGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGtaataa                                                                                                                                                                                                              >  1:4449/1‑220 (MQ=255)                                                            cGAAATCCGCGAATGCGGGATGTTATTGCGAAAGCCAGAAAATTTGCGTTATTGGATGCGCCGTTGGTGATTCAAGGAGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATg                                                                                                                                                                              >  1:43367/1‑250 (MQ=255)                                                                       aaTGCGGGATGTTATTGCGAAAGCCAGAAAATTTGCGTTATTGGATGCGCCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATggcggc                                                                                                                                                                   <  1:308643/250‑1 (MQ=255)                                                                               aTGTTATTGCGAAAGCCAGAAAATTTGCGTTATTGGATGCGCCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGtgttg                                                                                                                                                          >  1:230715/1‑251 (MQ=255)                                                                                 gTTATTGCGAAAGCCAGAAAATTTGCGTTATTGGATGCGCCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTgttgtt                                                                                                                                                        >  1:702109/1‑251 (MQ=255)                                                                                  ttATTGCGAAAGCCAGAAAATTTGCGTTATTGGATGCGCCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTgttgtt                                                                                                                                                        <  1:812201/250‑1 (MQ=255)                                                                                   tATTGCGAAAGCCAGAAAATTTGCGTTATTGGATGCGCCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAg                                                                                                                                                      <  1:508663/251‑1 (MQ=255)                                                                                    aTTGCGAAAGCCAGAAAATTTGCGTTATTGGATGCGCCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGa                                                                                                                                                     <  1:831619/251‑1 (MQ=255)                                                                                                  aaaaTTTGCGTTATTGGATGCGCCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTgagg                                                                                                                                          <  1:759537/248‑1 (MQ=255)                                                                                                  aaaaTTTGCGTTATTGGATGCGCCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTgagg                                                                                                                                          <  1:751281/248‑1 (MQ=255)                                                                                                  aaaaTTTGCGTTATTGGATGCGCCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGACGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTgagg                                                                                                                                          <  1:483111/248‑1 (MQ=255)                                                                                                           gTTATTGGATGCGCCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGCGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGa                                                                                                                              >  1:314756/1‑251 (MQ=255)                                                                                                            ttATTGGATGCGCCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGAc                                                                                                                             <  1:714812/251‑1 (MQ=255)                                                                                                                tGGATGCGCCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTg                                                                                                                         >  1:606767/1‑251 (MQ=255)                                                                                                                      cgcCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCg                                                                                                                   >  1:524759/1‑251 (MQ=255)                                                                                                                        ccGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAg                                                                                                                                                      <  1:863589/214‑1 (MQ=255)                                                                                                                            tGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCtt                                                                                                             >  1:789145/1‑251 (MQ=255)                                                                                                                             ggTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAg                                                                                                                                                                                                            >  1:497107/1‑155 (MQ=255)                                                                                                                             ggTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCtt                                                                                                             >  1:387428/1‑250 (MQ=255)                                                                                                                                gATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCt                                                                                                              >  1:133470/1‑246 (MQ=255)                                                                                                                                gATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCt                                                                                                              >  1:83815/1‑246 (MQ=255)                                                                                                                                                     gggAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCAc                                                                                                                                                                 >  1:505379/1‑174 (MQ=255)                                                                                                                                                      ggAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTgagg                                                                                                                                          <  1:121670/196‑1 (MQ=255)                                                                                                                                                      ggAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCTTCTGCGTTTTTTAAGCGACGGTACttt                                                                                   >  1:351474/1‑251 (MQ=255)                                                                                                                                                           aGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCTTCTGCGTTTTTTAAGCGACGGTACTTTccgc                                                                               >  1:42230/1‑250 (MQ=255)                                                                                                                                                                    tGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCTTCTGCGTTTTTTAAGCGACGGTACTTTCCGCCGTGTCGgag                                                                     <  1:237125/251‑1 (MQ=255)                                                                                                                                                                     gCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTa                                                                                                                                                       <  1:847166/168‑1 (MQ=255)                                                                                                                                                                     gCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCTTCTGCGTTTTTTAAGCGACGGTACTTTcc                                                                                 >  1:529709/1‑238 (MQ=255)                                                                                                                                                                     gCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCTTCTGCGTTTTTTAAGCGACGGTACTTTCCGCCGTGTCGgaga                                                                    >  1:211821/1‑251 (MQ=255)                                                                                                                                                                      ccAACGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCTTCTGCGTTTTTTAAGCGACGGTACTTTCCGCCGTGTCGGagag                                                                   <  1:46989/251‑1 (MQ=255)                                                                                                                                                                           gCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCTTCTGCGTTTTTTAAGCGACGGTACTTTCCGCCGTGTCGGagaggaaga                                                              >  1:446653/1‑251 (MQ=255)                                                                                                                                                                             cTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCTTCTGCGTTTTTTAAGCGACGGTACTTTCCGCCGTGTCGGagaggaagagg                                                            <  1:234806/251‑1 (MQ=255)                                                                                                                                                                                    gATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTgttgtt                                                                                                                                                        >  1:814615/1‑152 (MQ=255)                                                                                                                                                                                     aTTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTgaggag                                                                                                                                        <  1:347344/167‑1 (MQ=255)                                                                                                                                                                                     aTTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCTTCTGCGTTTTTTAAGCGACGGTACTTTCCGCCGTGTCGGAGAGGAAGAGGAGCATTAt                                                    >  1:196104/1‑251 (MQ=255)                                                                                                                                                                                     aTTTTAGTGCACGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCTTCTGCGTTTTTTAAGCGACGGTACTTTCCGCCGTGTCGGAGAGGAAGAGGAGCATTAt                                                    >  1:640540/1‑251 (MQ=255)                                                                                                                                                                                      ttttAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCTTCTGCGTTTTTTAAGCGACGGTACTTTCCGCCGTGTCGGagaggaagagg                                                            <  1:734795/242‑1 (MQ=255)                                                                                                                                                                                              cgcgcCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGGATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCTTCTGCGTTTTTTAAGCGACGGTACTTTCCGCCGTGTCGGAGAGGAAGAGGAGCATTATGCCAATGTa                                           <  1:744221/251‑1 (MQ=255)                                                                                                                                                                                              cgcgcCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCTTCTGCGTTTTTTAAGCGACGGTACTTTCCGCCGTGTCGGAGAGGAAGAGGAGCATTATGCCAATGTa                                           <  1:181382/251‑1 (MQ=255)                                                                                                                                                                                                     aaaTAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATTGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCTTCTGCGTTTTTTAAGCGACGGTACTTTCCGCCGTGTCGGAGAGGAAGAGGAGCATTATGc                                                  >  1:826895/1‑237 (MQ=255)                                                                                                                                                                                                                     gCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCTTCTGCGTTTTTTAAGCGACGGTACTTTCCGCCGTGTCGGAGAGGAAGAGGAGCATTATGCCAATg                                             <  1:29505/226‑1 (MQ=255)                                                                                                                                                                                                                     gCCGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCTTCTGCGTTTTTTAAGCGACGGTACTTTCCGCCGTGTCGGAGAGGAAGAGGAGCATTATGCCAATGTACGTGTGATTTGCACGGCgcagca                    >  1:589633/1‑251 (MQ=255)                                                                                                                                                                                                                      ccGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGa                                                                                                                                                     >  1:772027/1‑121 (MQ=255)                                                                                                                                                                                                                      ccGTAAACTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCTTCTGCGTTTTTTAAGCGACGGTACTTTCCGCCGTGTCGGAGAGGAAGAGGAGCATTATGCCAATGTACGTGTGATTTGCACGGCGcagcag                   <  1:26106/251‑1 (MQ=255)                                                                                                                                                                                                                          aaaCTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCTTCTGCGTTTTTTAAGCGACGGTACTTTCCGCCGTGTCGGAGAGGAAGAGGAGCAt                                                       >  1:838197/1‑211 (MQ=255)                                                                                                                                                                                                                          aaaCTGTGCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCTTCTGCGTTTTTTAAGCGACGGTACTTTCCGCCGTGTCGGAGAGGAAGAGGAGCATTATGCCAATGTACGTGTGATTTGCACGGCGCAGCAGCCg                >  1:50560/1‑250 (MQ=255)                                                                                                                                                                                                                               gtgCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCTTCTGCGTTTTTTAAGCGACGGTACTTTCCGCCGTGTCGGAGAGGAAGAGGAGCATTATGCCAATGTACGTGTGa                                    >  1:273343/1‑225 (MQ=255)                                                                                                                                                                                                                               gtgCCGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCTTCTGCGTTTTTTAAGCGACGGTACTTTCCGCCGTGTCGGAGAGGAAGAGGAGCATTATGCCAATGTACGTGTGATTTGCACGGCGCAGCAGCCGTTACAg          >  1:323699/1‑251 (MQ=255)                                                                                                                                                                                                                                  ccGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCTTCTGCGTTTTTTAAGCGACGGTACTTTCCGCCGTGTCGGAGAGGATGAGGAGCATTATGCCAATGTACGTGTGATTTGCACGGCGCAGCAGCCGTTACAGCAt       >  1:378434/1‑251 (MQ=255)                                                                                                                                                                                                                                   cGGCATTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCTTCTGCGTTTTTTAAGCGACGGTACTTTCCGCCGTGTCGGAGAGGAAGAGGAGCATTATGCCAATGTACGTGTGATTTGCACGGCGCAGCAGCCGTTACAGCAtt      >  1:188031/1‑251 (MQ=255)                                                                                                                                                                                                                                       aTTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCTTCTGCGTTTTTTAAGCGACGGTACTTTCCGCCGTGTCGGAGAGGAAGAGGAGCATTATGCCAATGTACGTGTGATTTGCACGGCGCAGCAGCCGTTACAGCATTATGt  <  1:462010/251‑1 (MQ=255)                                                                                                                                                                                                                                       aTTCCCGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCTTCTGCGTTTTTTAAGCGACGGTACTTTCCGCCGTGTCGGAGAGGAAGAGGAGCATTATGCCAATGTACGTGTGATTTGCACGGCGCAGCAGCCGTTACAGCATTATGt  <  1:496765/251‑1 (MQ=255)                                                                                                                                                                                                                                          cccGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCgg                                                                                                                                                                                            <  1:108358/62‑1 (MQ=255)                                                                                                                                                                                                                                          cccGGCGAAGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCTTCTGCGTTTTTTAAGCGACGGTACTTTCCGCCGTGTCGGAGAGGAAGAGGAGCATTATGCCAATGTACGTGTGATTTGCACGGCGCAGCAGCCGTTACAg          <  1:884947/240‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                TTATTGAGGCACTTATGGCAACTTCTTCAACCACCGATCCTTTTTCACACATCGTCAGCCGAAATCCGCGAATGCGGGATGTTATTGCGAAAGCCAGAAAATTTGCGTTATTGGATGCGCCGTTGGTGATTCAAGGTGAAACCGGCACGGGAAAAGACGTGATTGCCAAAGCCTGCCATGATTTTAGTGCGCGCCGAAATAAAGCATTTCTCGCCGTAAACTGTGCCGGCATTCCCGGCGAGGACGCGGAAACGGAAATGTTCGGGCGGCGTAATAAAGATGGTGAATTTATCGGTTTTTTTGAATATGCCGATGGCGGCACGGTGTTGTTAGATGGTGTTGAGGAGTTGCCTCTGACTTTGCAGGCGAAGCTTCTGCGTTTTTTAAGCGACGGTACTTTCCGCCGTGTCGGAGAGGAAGAGGAGCATTATGCCAATGTACGTGTGATTTGCACGGCGCAGCAGCCGTTACAGCATTATGT  >  1/683989‑684469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑