Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	681,507	A→C	R33R (AGA→CGA)	unknown →	Conserved protein YcjX with nucleoside triphosphate hydrolase domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	681,507	0	A	C	100.0%	159.4 / NA	58	R33R (AGA→CGA)	unknown	Conserved protein YcjX with nucleoside triphosphate hydrolase domain
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (28/30);  total (28/30)

CTTAGTATTCACCGTAGAAAATAACCGCACTTTTTCGGGAGAAACGTTGTTAATTTAAGCCTCTCTTCATTTTTTCAGTATAATATTCGTCATGATTATTGCACTATTGCACAAATACTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTAGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGCGGTAAAACGTATTCCTCAACCGGATTTATCCGTTCCCCGTTTTGATTATGATGGCAATCTCAATGCCTTGTCGCAGCAACCGCCTGTTTGGCCGCAATCTACCAGAAGTGTAAGCGAAACCCGACTGG  >  1/681287‑681750                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    cTTAGTATTCACCGTAGAAAATAACCGCACTTTTTCGGGAGAAACGTTGTTAATTTAAGCCTCTCTTCATTTTTTCAGTATAATATTCGTCATGATTATTGCACTATTGCACAAATACTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGcc                                                                                                                                                                                                                       <  1:899352/251‑1 (MQ=255) cTTAGTATTCACCGTAGAAAATAACCGCACTTTTTCGGGAGAAACGTTGTTAATTTAAGCCTCTCTTCATTTTTTCAGTATAATATTCATCATGATTATTGCACTATTGCACAAATACTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGcc                                                                                                                                                                                                                       <  1:517692/251‑1 (MQ=255)  ttAGTATTCACCGTAGAAAATAACCGCACTTTTTCGGGAGAAACGTTGTTAATTTAAGCCTCTCTTCATTTTTTCAGTATAATATTCGTCATGATTATTGCACTATTGCACAAATACTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGc                                                                                                                                                                                                                        >  1:235432/1‑249 (MQ=255)               tAGAAAATAACCGCACTTTTTCGGGAGAAACGTTGTTAATTTAAGCCTCTCTTCATTTTTTCAGTATAATATTCGTCATGATTATTGCACTATTGCACAAATACTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTg                                                                                                                                                                                                         <  1:827010/251‑1 (MQ=255)                 gAAAATAACCGCACTTTTTCGGGAGAAACGTTGTTAATTTAAGCCTCTCTTCATTTTTTCAGTATAATATTCGTCATGATTATTGCACTATTGCACAAATACTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGtt                                                                                                                                                                                                       >  1:412808/1‑251 (MQ=255)                      tAACCGCACTTTTTCGGGAGAAACGTTGTTAATTTAAGCCTCTCTTCATTTTTTCAGTATAATATTCGTCATGATTATTGCACTATTGCACAAATACTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACata                                                                                                                                                                                                  >  1:792250/1‑251 (MQ=255)                       aaCCGCACTTTTTCGGGAGAAACGTTGTTAATTTAAGCCTCTCTTCATTTTTTCAGTATAATATTCGTCATGATTATTGCACTATTGCACAAATACTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACatat                                                                                                                                                                                                 <  1:866314/251‑1 (MQ=255)                            cACTTTTTCGGGAGAAACGTTGTTAATTTAAGCCTCTCTTCATTTTTTCAGTATAATATTCGTCATGATTATTGCACTATTGCACAAATACTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAAt                                                                                                                                                                                             <  1:175014/250‑1 (MQ=255)                            cACTTTTTCGGGAGAAACGTTGTTAATTTAAGCCTCTCTTCATTTTTTCAGTATAATATTCGTCATGATTATTGCACTATTGCACAAATACTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAAt                                                                                                                                                                                             >  1:508358/1‑250 (MQ=255)                             aCTTTTTCGGGAGAAACGTTGTTAATTTAAGCCTCTCTTCATTTTTTCAGTATAATATTCGTCATGATTATTGCACTATTGCACAAATACTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATcg                                                                                                                                                                                           <  1:370145/251‑1 (MQ=255)                                          aaaCGTTGTTAATTTAAGCCTCTCTTCATTTTTTCAGTATAATATTCGTCATGATTATTGCACTATTGCACAAATACTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGc                                                                                                                                                                              <  1:200821/251‑1 (MQ=255)                                                 gttAATTTAAGCCTCTCTTCATTTTTTCAGTATAATATTCGTCATGATTATTGCACTATTGCACAAATACTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTg                                                                                                                                                                        >  1:530074/1‑250 (MQ=255)                                                                         tttCAGTATAATATTCGTCATGATTATTGCACTATTGCACAAATACTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCTCTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGc                                                                                                                                                                       <  1:255864/227‑1 (MQ=255)                                                                                   atatTCGTCATGATTATTGCACTATTGCACAAATGCTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGattat                                                                                                                                     >  1:543821/1‑251 (MQ=255)                                                                                   atatTCGTCATGATTATTGCACTATTGCACAAATACTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGAt                                                                                                                                                            <  1:781386/228‑1 (MQ=255)                                                                                   atatTCGTCATGATTATTGCACTATTGCACAAATACTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGattat                                                                                                                                     >  1:574639/1‑251 (MQ=255)                                                                                     atTCGTCATGATTATTGCACTATTGCACAAATACTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTg                                                                                                                                   <  1:397174/251‑1 (MQ=255)                                                                                         gTCATGATTATTGCACTATTGCACAAATACTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGc                                                                                                                                                                              <  1:38465/204‑1 (MQ=255)                                                                                                ttattGCACTATTGCACAAATACTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTa                                                                                                                                                                                                      >  1:887475/1‑173 (MQ=255)                                                                                                ttattGCACTATTGCACAAATACTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAg                                                                                                                                                                               <  1:483899/196‑1 (MQ=255)                                                                                                ttattGCACTATTGCACAAATACTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTgggg                                                                                                                                <  1:478968/243‑4 (MQ=255)                                                                                                     gcactattgcacAAATACTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGGGGTAAAACGTATTc                                                                                                                    >  1:847214/1‑250 (MQ=255)                                                                                                          attgcacAAATACTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGattatt                                                                                                                                    <  1:458757/229‑1 (MQ=255)                                                                                                          attgcacAAATACTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGat                                                                                                                                        <  1:587870/225‑1 (MQ=255)                                                                                                             gcacAAATACTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGc                                                                                                                                                                    <  1:227187/194‑1 (MQ=255)                                                                                                             gcacAAATACTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGGGGTAAAACGTATTCCTCAACCgg                                                                                                           >  1:761262/1‑251 (MQ=255)                                                                                                                caAATACTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGGGGTAAAACGTATTCCTCAACCGGAtt                                                                                                        <  1:817119/251‑1 (MQ=255)                                                                                                                  aaTACTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTg                                                                                                                                   <  1:696690/222‑1 (MQ=255)                                                                                                                     aCTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGGGGTAAAACGTATTCCTCAACCGGATTTATcc                                                                                                   <  1:721501/251‑1 (MQ=255)                                                                                                                        gaagaaTGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACa                                                                                                                                                                                                    <  1:527467/151‑1 (MQ=255)                                                                                                                            aaTGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGGGGTAAAACGTATTCCTCAACCGGATTTATCCGTTccc                                                                                             >  1:766553/1‑250 (MQ=255)                                                                                                                            aaTGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGGGGTAAAACGTATTCCTCAACCGGATTTATCCGTTccc                                                                                             >  1:750100/1‑250 (MQ=255)                                                                                                                                 aaaCCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGGGGTAAAACGTATTCCTCAACCGGATTTATCCGTTc                                                                                               >  1:598467/1‑243 (MQ=255)                                                                                                                                        gCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGGGGTAAAACGTATTCCTCAACCGGATTTATCCGTTCCCCGTTTTGATTatg                                                                                >  1:810903/1‑251 (MQ=255)                                                                                                                                                   tGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGGGGTAAAACGTATTCCTCAACCGGATTTATCCGTTCCCCGTTTTGATTATGATGGCAAtct                                                                      >  1:745787/1‑250 (MQ=255)                                                                                                                                                        aaaGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGGGGTAAAACGTATTCCTCAACCGGATTTATCCGTTCCCCGTTTTGATTATGATGGCAATCTCAATGc                                                                <  1:31252/251‑1 (MQ=255)                                                                                                                                                                aaaCGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGGGGTAAAACGTATTCCTCAACCGGATTTATCCGTTCCCCGTTTTGATTATGATGGCAATCTCAATGCCTTGTCgc                                                        <  1:193988/251‑1 (MQ=255)                                                                                                                                                                               tCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGGGGTAAAACGTATTCCTCAACCGGATTTATCCGTTCCCCGTTTTGATTATGATGGCAATCTCAATGCCTTGTCGCAGCAACCGCCTGttt                                         >  1:705659/1‑251 (MQ=255)                                                                                                                                                                                  cgGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGGGGTAAAACGTATTCCTCAACCGGATTTATCCGTTCCCCGTTTTGATTATGATGGCAATCTCAATGCCTTGTCGCAGCAACCGCCTGTTTGGc                                      <  1:748999/251‑1 (MQ=255)                                                                                                                                                                                   ggATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGGGGTAAAACGTATTCCTCAACCGGATTTATCCGTTCCCCGTTTTGATTATGATGGCAATCTCAATGCCTTGTCGCAGCAACCGCCTGTTTGGc                                      >  1:44742/1‑250 (MQ=255)                                                                                                                                                                                        aGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTgggg                                                                                                                                >  1:475188/1‑152 (MQ=255)                                                                                                                                                                                        aGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGGGGTAAAACGTATTCCTCAACCGGATTTATCCGTTCCCCGTTTTGATTATGATGGCAATCTCAATGCCTTGTCGCAGCAACCGCCTGTTTGGCCGCAAt                                >  1:208809/1‑251 (MQ=255)                                                                                                                                                                                        aGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGGGGTAAAACGTATTCCTCAACCGGATTTATCCGTTCCCCGTTTTGATTATGATGGCAATCTCAATGCCTTGTCGCAGCAACCGCCTGTTTGGCCGCAAt                                >  1:13763/1‑251 (MQ=255)                                                                                                                                                                                         gACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGGGGTAAAACGTATTCCTCAACCGGATTTATCCGTTCCCCGTTTTGATTATGATGGCAATCTCAATGCCTTGTCGCAGCAACCGCCTGTTTGGCCGCAAt                                >  1:362357/1‑250 (MQ=255)                                                                                                                                                                                              cACTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGGGGTAAAACGTATTCCTCAACCGGATTTATCCGTTCCCCGTTTTGATTATGATGGCAATCTCAATGCCTTGTCGCAGCAACCGCCTGTTTGGCCGCAATCTAcc                           <  1:2111/250‑1 (MQ=255)                                                                                                                                                                                                cTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGGGGTAAAACGTATTcc                                                                                                                   >  1:368962/1‑160 (MQ=255)                                                                                                                                                                                                cTTTACGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGGGGTAAAACGTATTCCTCAACCGGATTTATCCGTTCCCCGTTTTGATTATGATGGc                                                                           >  1:779874/1‑200 (MQ=255)                                                                                                                                                                                                    aCGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCCCCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGGGGTAAAACGTATTCCTCAACCGGATTTATCCGTTCCCCGTTTTGATTATGATGGCAATCTCAATGCCTTGTCGCAGCAACCGCCTGTTTGGCCGCAATCTACCAGAAgtg                    <  1:299655/251‑1 (MQ=255)                                                                                                                                                                                                    aCGCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGGGGTAAAACGTATTCCTCAACCGGATTTATCCGTTCCCCGTTTTGATTATGATGGCAATCTCAATGCCTTGTCGCAGCAACCGCCTGTTTGGCCGCAATCTACCAGAAgtg                    >  1:581315/1‑251 (MQ=255)                                                                                                                                                                                                      gCATTGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGGGGTAAAACGTATTCCTCAACCGGATTTATCCGTTCCCCGTTTTGATTa                                                                                  <  1:706827/187‑1 (MQ=255)                                                                                                                                                                                                         ttGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGGGGTAAAACGTATTCCTCAACCGGATTTATCCGTTCCCCGTTTTGATTATGATGGCAATCTCAATGCCTTGTCGCAGCAACCGCCTGTTTGGCCGCAATCTACCAGAAGTGTAAg                >  1:736783/1‑250 (MQ=255)                                                                                                                                                                                                          tGCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACatat                                                                                                                                                                                                 <  1:413790/72‑1 (MQ=255)                                                                                                                                                                                                           gCTGTGACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTg                                                                                                                                   >  1:732493/1‑133 (MQ=255)                                                                                                                                                                                                                gACAGGGTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGGGGTAAAACGTATTCCTCAACCGGATTTATCCGTTCCCCGTTTTGATTATGATGGc                                                                           <  1:663876/184‑1 (MQ=255)                                                                                                                                                                                                                    gggTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGGGGTAAAACGTATTCCTCAACCGGATTTATCCGTTCCCCGTTTTGATTATGATGGCAATCTCAATGCCTTGTCGCAGCAACCGCCTGTTTGGCCGCAATCTACCAGAAGTGTAAGCGAAACCCGa      >  1:628848/1‑249 (MQ=255)                                                                                                                                                                                                                    gggTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGGGGTAAAACGTATTCCTCAACCGGATTTATCCGTTCCCCGTTTTGATTATGATGGCAATCTCAATGCCTTGTCGCAGCAACCGCCTGTTTGGCCGCAATCTACCAGAAGTGTAAGCGAAACCCGa      >  1:794824/1‑249 (MQ=255)                                                                                                                                                                                                                     ggTTGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGGGGTAAAACGTATTCCTCAACCGGATTTATCCGTTCCCCGTTTTGATTatgat                                                                              <  1:394724/176‑1 (MQ=255)                                                                                                                                                                                                                       ttGAGTCGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGGGGTAAAACGTATTCCTCAACCGGATTTATCCGTTCCCCGTTTTGATTATGATGGCAATCTCAATGCCTTGTCGCAGCAACCGCCTGTTTGGCCGCAATCTACCAGAAGTGTAAGCGAAACCCGACTgg  <  1:60422/250‑1 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    CTTAGTATTCACCGTAGAAAATAACCGCACTTTTTCGGGAGAAACGTTGTTAATTTAAGCCTCTCTTCATTTTTTCAGTATAATATTCGTCATGATTATTGCACTATTGCACAAATACTGAAGAATGAAAACCATGCTAAATCATTTGCAAAAAGAATTAAACGATCTGGTTAATCGCGGATTAGACCGCACTTTACGCATTGCTGTGACAGGGTTGAGTAGAAGCGGGAAAACTGCTTTTATCACCAGCCTTATTAACCAATTGTTACATATCAATCGCGTGGATAATAGCCATCTGCCGCTCTTTGATGCGGCGCGGCGGCGCGGGATTATTGCGGTAAAACGTATTCCTCAACCGGATTTATCCGTTCCCCGTTTTGATTATGATGGCAATCTCAATGCCTTGTCGCAGCAACCGCCTGTTTGGCCGCAATCTACCAGAAGTGTAAGCGAAACCCGACTGG  >  1/681287‑681750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑