Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	679,523	T→C	G561G (GGA→GGG)	unknown ←	Peptide ABC transporter, substrate‑binding protein SapA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	679,523	0	T	C	100.0%	227.4 / NA	81	G561G (GGA→GGG)	unknown	Peptide ABC transporter, substrate‑binding protein SapA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (46/35);  total (46/35)

TGGGGGGTAAAACAGTAAAAATCGTGGATTTTAACAGCTGCCCGCCATTGTAACTGATACCTAAATCACCACGGGTAAGGTTGCCTAAATAATTAAGGTAGCCATTGTAAAAATACGGTGTCGCCAGTTCGGCATTTAATGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGTCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAAGCAGCGGAGCGTACGCGCTGATTGGAAGTATCCAATGCCTCATCCATAATTTGATCAAATTTTTCGCTGCACCAGTTGGATAAGTTGGTAA  >  1/679283‑679767                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     tGGGGGGTAAAACAGTAAAAATCGTGGATTTTAACAGCTGCCCGCCATTGTAACTGATACCTAAATCACCACGGGTAAGGTTGCCTAAATAATTAAGGTAGCCATTGTAAAAATACGGTGTCGCCAGTTCGGCATTTAATGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGc                                                                                                                                                                                                                                             <  1:114890/250‑1 (MQ=255)    ggggTAAAACAGTAAAAATCGTGGATTTTAACAGCTGCCCGCCATTGTAACTGATACCTAAATCACCACGGGTAAGGTTGCCTAAATAATTAAGGTAGCCATTGTAAAAATACGGTGTCGCCAGTTCGGCATTTAATGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAggg                                                                                                                                                                                                                                         >  1:393136/1‑251 (MQ=255)                aaaaaaTCGTGGATTTTAACAGCTGCCCGCCATTGTAACTGATACCTAAATCACCACGGGTAAGGTTGCCTAAATAATTAAGGTAGCCATTGTAAAAATACGGTGTCGCCAGTTCGGCATTTAATGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGaaa                                                                                                                                                                                                                             <  1:559132/250‑1 (MQ=255)                        gTGGATTTTAACAGCTGCCCGCCATTGTAACTGATACCTAAATCACCACGGGTAAGGTTGCCTAAATAATTAAGGTAGCCATTGTAAAAATACGGTGTCGCCAGTTCGGCATTTAATGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAgg                                                                                                                                                                                                                     >  1:517841/1‑251 (MQ=255)                                      cTGCCCGCCATTGTAACTGATACCTAAATCACCACGGGTAAGGTTGCCTAAATAATTAAGGTAGCCATTGTAAAAATACGGTGTCGCCAGTTCGGCATTTAATGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACgg                                                                                                                                                                                                          <  1:260105/248‑1 (MQ=255)                                             ccATTGTAACTGATACCTAAATCACCACGGGTAAGGTTGCCTAAATAATTAAGGTAGCCATTGTAAAAATACGGTGTCGCCAGTTCGGCATTTAATGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCg                                                                                                                                                                                                >  1:413387/1‑251 (MQ=255)                                             ccATTGTAACTGATACCTAAATCACCACGGGTAAGGTTGCCTAAATAATTAAGGTAGCCATTGTAAAAATACGGTGTCGCCAGTTCGGCATTTAATGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCAAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCg                                                                                                                                                                                                >  1:448231/1‑251 (MQ=255)                                                ttGTAACTGATACCTAAATCACCACGGGTAAGGTTGCCTAAATAATTAAGGTAGCCATTGTAAAAATACGGTGTCGCCAGTTCGGCATTTAATGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGt                                                                                                                                                                                                  >  1:495794/1‑246 (MQ=255)                                                   tAACTGATACCTAAATCACCACGGGTAAGGTTGCCTAAATAATTAAGGTAGCCATTGTAAAAATACGGTGTCGCCAGTTCGGCATTTAATGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATAttt                                                                                                                                                                                          <  1:371731/251‑1 (MQ=255)                                                            ccTAAATCACCACGGGTAAGGTTGCCTAAATAATTAAGGTAGCCATTGTAAAAATACGGTGTCGCCAGTTCGGCATTTAATGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGaaa                                                                                                                                                                                                                             <  1:879656/207‑1 (MQ=255)                                                                aaTCACCACGGGTAAGGTTGCCTAAATAATTAAGGTAGCCATTGTAAAAATACGGTGTCGCCAGTTCGGCATTTAATGGATTCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAAc                                                                                                                                                                             <  1:423286/251‑1 (MQ=255)                                                                         gggTAAGGTTGCCTAAATAATTAAGGTAGCCATTGTAAAAATACGGTGTCGCCAGTTCGGCATTTAATGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGc                                                                                                                                                                                                                                             <  1:313372/178‑1 (MQ=255)                                                                         gggTAAGGTTGCCTAAATAATTAAGGTAGCCATTGTAAAAATACGGTGTCGCCAGTTCGGCATTTAATGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGt                                                                                                                                                                                                  >  1:511074/1‑221 (MQ=255)                                                                         gggTAAGGTTGCCTAAATAATTAAGGTAGCCATTGTAAAAATACGGTGTCGCCAGTTCGGCATTTAATGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGcgacga                                                                                                                                                                    >  1:659608/1‑251 (MQ=255)                                                                         gggTAAGGTTGCCTAAATAATTAAGGTAGCCATTGTAAAAATACGGTGTCGCCAGTTCGGCATTTAATGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGcgacga                                                                                                                                                                    >  1:146247/1‑251 (MQ=255)                                                                                          taatTAAGGTAGCCATTGTAAAAATACGGTGTCGCCAGTTCGGCATTTAATGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGtt                                                                                                                                                    >  1:678307/1‑250 (MQ=255)                                                                                           aatTAAGGTAGCCATTGTAAAAATACGGTGTCGCCAGTTCGGCATTTAATGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTcg                                                                                                                                                  >  1:222816/1‑251 (MQ=255)                                                                                           aatTAAGGTAGCCATTGTAAAAATACGGTGTCGCCAGTTCGGCATTTAATGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTcg                                                                                                                                                  >  1:645259/1‑251 (MQ=255)                                                                                               aaGGTAGCCATTGTAAAAATACGGTGTCGCCAGTTCGGCATTTAATGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTAAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACAcgcg                                                                                                                                                                                <  1:289363/217‑1 (MQ=255)                                                                                                          tGTAAAAATACGGTGTCGCCAGTTCGGCATTTAATGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTg                                                                                                                                   <  1:680299/251‑1 (MQ=255)                                                                                                           gTAAAAATACGGTGTCGCCAGTTCGGCATTTAATGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGt                                                                                                                                                                                                  <  1:212521/187‑1 (MQ=255)                                                                                                           gTAAAAATACGGTGTCGCCAGTTCGGCATTTAATGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTgg                                                                                                                                  >  1:176056/1‑251 (MQ=255)                                                                                                           gTAAAAATACGGTGTCGCCAGTTCGGCATTTAATGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTgg                                                                                                                                  >  1:376333/1‑251 (MQ=255)                                                                                                           gTAAAAATACGGTGTCGCCAGTTCGGCATTTAATGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGG‑‑GAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTa                                                                                                                                >  1:828246/1‑251 (MQ=255)                                                                                                               aaaTACGGTGTCGCCAGTTCGGCATTTAATGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAAc                                                                                                                              <  1:34018/251‑1 (MQ=255)                                                                                                                    cGGTGTCGCCAGTTCGGCATTTAATGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTg                                                                                                                                   <  1:597104/241‑1 (MQ=255)                                                                                                                                    gCATTTAATGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGt                                                                                                             >  1:500279/1‑247 (MQ=255)                                                                                                                                      aTTTAATGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGc                                                                                                       <  1:422999/251‑1 (MQ=255)                                                                                                                                           aTGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGaaa                                                                                                                                                                                                                             >  1:101002/1‑128 (MQ=255)                                                                                                                                               aTCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAAcc                                                                                                                                                               >  1:36882/1‑186 (MQ=255)                                                                                                                                               aTCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATtata                                                                                              >  1:537614/1‑251 (MQ=255)                                                                                                                                                  cTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAagc                                                                                           <  1:619235/251‑1 (MQ=255)                                                                                                                                                  cTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAagc                                                                                           <  1:840619/251‑1 (MQ=255)                                                                                                                                                  cTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAagc                                                                                           <  1:529384/251‑1 (MQ=255)                                                                                                                                                  cTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAagc                                                                                           <  1:467289/251‑1 (MQ=255)                                                                                                                                                  cTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAagc                                                                                           <  1:142979/251‑1 (MQ=255)                                                                                                                                                    gCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAAc                                                                                                                                                       <  1:263947/189‑1 (MQ=255)                                                                                                                                                     cATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAagcag                                                                                         >  1:549905/1‑250 (MQ=255)                                                                                                                                                     cATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAagcag                                                                                         >  1:271487/1‑250 (MQ=255)                                                                                                                                                             tAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACgag                                                                                                                                                                   >  1:125858/1‑168 (MQ=255)                                                                                                                                                              aaCGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAagc                                                                                           >  1:821750/1‑239 (MQ=255)                                                                                                                                                                 gTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACg                                                                                                              <  1:39793/217‑1 (MQ=255)                                                                                                                                                                  tAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAAGCAGCGGAGCGTAcgcg                                                                            >  1:692080/1‑250 (MQ=255)                                                                                                                                                                  tAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAAGCAGCGGAGCGTAcgcg                                                                            >  1:862204/1‑250 (MQ=255)                                                                                                                                                                      ttATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTACCGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAAGCAGCGGAGCGTACGCGCTGAt                                                                       >  1:434476/1‑251 (MQ=255)                                                                                                                                                                        aTCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAAGCAGCGGAGCGTACGCGCTGATTg                                                                     >  1:808246/1‑251 (MQ=255)                                                                                                                                                                        aTCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAAGCAGCGGAGCGTACGCGCTGATTg                                                                     <  1:370959/251‑1 (MQ=255)                                                                                                                                                                            aTGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACg                                                                                                                                                      >  1:87207/1‑166 (MQ=255)                                                                                                                                                                            aTGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAAc                                                                                                                              <  1:71162/190‑1 (MQ=255)                                                                                                                                                                             tgtgAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACg                                                                                                                                                      >  1:804202/1‑165 (MQ=255)                                                                                                                                                                                gAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGt                                                                                                                                                                                                  >  1:683708/1‑118 (MQ=255)                                                                                                                                                                                  ggATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAAGCAGCGGAGCGCACGCGCTGCTTGGAAGTATCCa                                                           >  1:463987/1‑251 (MQ=255)                                                                                                                                                                                   gATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAAGCAGCGGAGCGTACGCGCTGATTGGAAGTATcc                                                            <  1:696260/249‑1 (MQ=255)                                                                                                                                                                                   gATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAAGCAGCGGAGCGTACGCGCTGATTGGAAGTATcc                                                            <  1:285894/249‑1 (MQ=255)                                                                                                                                                                                   gATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAAGCAGCGGAGCGTACGCGCTGATTGGAAGTATCCaa                                                          >  1:227050/1‑251 (MQ=255)                                                                                                                                                                                     tAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTaa                                                                                                                               >  1:741671/1‑180 (MQ=255)                                                                                                                                                                                     tAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAAGCAGCGGAGCGTACGCGCTGATTGGAAGTATCCAATg                                                        >  1:418455/1‑251 (MQ=255)                                                                                                                                                                                      aaTTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAAGCAGCGGAGCGTACGCGCTGATTGGAAGTATCCAATGc                                                       <  1:513328/251‑1 (MQ=255)                                                                                                                                                                                            cGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAAGCAGCGGAGCGTACGCGCTGATTGGAAGTATCCAATGCCTCATc                                                 <  1:372633/251‑1 (MQ=255)                                                                                                                                                                                             gTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATtat                                                                                               >  1:710551/1‑204 (MQ=255)                                                                                                                                                                                                   aCGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAAGCAGCGGAGCGTACGCGCTGATTGGAAGTATCCAATGCCTCATCCATAAtt                                          <  1:138344/251‑1 (MQ=255)                                                                                                                                                                                                     gagaAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGGTGAAAAGTTCAGGCTACCGAAc                                                                                                                                                                                                            >  1:362284/1‑87 (MQ=255)                                                                                                                                                                                                                 ttGCCGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAAGCAGCGGAGCGTACGCGCTGATTGGAAGTATCCAATGCCTCATCCATAAtt                                          >  1:412363/1‑237 (MQ=255)                                                                                                                                                                                                                    ccGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAAGCAGCGGAGCGTACGCGCTGATTGGAAGTATCCAATGCCTCATCCATAATTTGATCAAATTTTTCGc                          <  1:690162/250‑1 (MQ=255)                                                                                                                                                                                                                    ccGAATCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTATGCCTGATTATAAGCAGCGGAGCGTACGCGCTGATTGGAAGTATCCAATGCCTCATCCATAAtt                                          >  1:443038/1‑234 (MQ=255)                                                                                                                                                                                                                       aaTCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAAGCAGCGGAGCGTACGCGCTGATTGGAAg                                                                 >  1:845223/1‑208 (MQ=255)                                                                                                                                                                                                                       aaTCAGTGAATTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAAGCAGCGGAGCGTACGCGCTGATTGGAAGTATCCAATGCCTCATCCATAATTTGATCAAATTTTTCGCTGCa                      >  1:596716/1‑251 (MQ=255)                                                                                                                                                                                                                                aTTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATGAGCAGCGGAGCGTACGCGCTGATTGGAAGTATCCAATGCCTCATcc                                                >  1:424425/1‑216 (MQ=255)                                                                                                                                                                                                                                aTTAAACATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAagc                                                                                           <  1:510367/173‑1 (MQ=255)                                                                                                                                                                                                                                   aaaCATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAAGCAGCGGAGCGTACGCGCTGATTGGAAGTATCCAATGCCTCATCCATAATTTGATCAAATTTTTCGCTGCACCAGt                 >  1:149498/1‑244 (MQ=255)                                                                                                                                                                                                                                   aaaCATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAAGCAGCGGAGCGTACGCGCTGATTGGAAGTATCCAATGCCTCATCCATAATTTGATCAAATTTTTCGCTGCACCAGTTGGATaa          >  1:238363/1‑251 (MQ=255)                                                                                                                                                                                                                                   aaaCATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAAGCAGCGGAGCGTACGCGCTGATTGGAAGTATCCAATGCCTCATCCATAATTTGATCAAATTTTTCGCTGCACCAGTTGGATaa          >  1:813157/1‑251 (MQ=255)                                                                                                                                                                                                                                    aaCATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAAGCAGCGGAGCGTACGCGCTGATTGGAAGTATCCAATGCCTCATCCATAATTTGATCAAATTTTTCGCTGCACCAGTTGGATAAg         <  1:154720/251‑1 (MQ=255)                                                                                                                                                                                                                                     aCATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAagc                                                                                           >  1:816357/1‑168 (MQ=255)                                                                                                                                                                                                                                     aCATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCT‑‑ACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAagc                                                                                           <  1:448995/166‑1 (MQ=255)                                                                                                                                                                                                                                      cATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAagc                                                                                           <  1:661872/167‑1 (MQ=255)                                                                                                                                                                                                                                      cATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAAGCAGCGGAGCGTACGCGCTGATTGGAAGTATCCAATGCCTCATCCATAATTTGATCAAATTTTTCGCTGCACCAGtt                <  1:129836/242‑1 (MQ=255)                                                                                                                                                                                                                                      cATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAAGCAGCGGAGCGTACGCGCTGATTGGAAGTATCCAATGCCTCATCCATAATTTGATCAAATTTTTCGCTGCACCAGTTGGATa           <  1:597416/247‑1 (MQ=255)                                                                                                                                                                                                                                      cATTATTTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAAGCAGCGGAGCGTACGCGCTGATTGGAAGTATCCAATGCCTCATCCATAATTTGATCAAATTTTTCGCTGCACCAGTTGGATAAGtt       <  1:661719/251‑1 (MQ=255)                                                                                                                                                                                                                                          attTGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGA‑TGGTAACTc                                                                                                                            >  1:540270/1‑129 (MQ=255)                                                                                                                                                                                                                                           tttGTGCCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAAGCAGCGGAGCGTACGCGCTGATTGGAAGTATCCAATGCCTCATCCATAATTTGATCAAATTTTTCGCTGCACCAGTTGGATAAGTTGGTaa  >  1:207960/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     TGGGGGGTAAAACAGTAAAAATCGTGGATTTTAACAGCTGCCCGCCATTGTAACTGATACCTAAATCACCACGGGTAAGGTTGCCTAAATAATTAAGGTAGCCATTGTAAAAATACGGTGTCGCCAGTTCGGCATTTAATGGATCCTGCATTAAAATAACGTAACTTATCAATGTGAGGATAATTGACGTTAAAACGAGAAAAACACATTGCCGAATCAGTGAATTAAACATTATTTGTGTCCTTTTCGCAGGGAGAGGGTTGAAAAGTTCAGGCTACCGAACGGACTCATGTCGATATTTTGAACACGCGAACTGGCGACGAGAACCCGCTTAACGTTCGCGATCGGAATGATTGGTAACTCCGATAAAATTAACGTCTGTGCCTGATTATAAGCAGCGGAGCGTACGCGCTGATTGGAAGTATCCAATGCCTCATCCATAATTTGATCAAATTTTTCGCTGCACCAGTTGGATAAGTTGGTAA  >  1/679283‑679767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑