Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	653,823	G→T	A305E (GCG→GAG)	unknown ←	Fe2+ ABC transporter, permease protein 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	653,823	0	G	T	100.0%	211.5 / NA	76	A305E (GCG→GAG)	unknown	Fe2+ ABC transporter, permease protein 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (41/35);  total (41/35)

GTTTTAGCCATGTTATTCCCCTTTTAACACATTGGCGGTTTGAATGTCTAAGGCGCGGCGGGTCGGTACGATAACTGCGATAAACGCCACTAATAAGGATAATCCGATGGTGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCGCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATCTTATCCAGAATTTGCCCTTCGGAGGCGGAGACTTTGCGGATCGGATGAATATCCAAATCGGGATATTGTTGCTGAAGTTGC  >  1/653585‑654065                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   gTTTTAGCCATGTTATTCCCCTTTTAACACATTGGCGGTTTGAATGTCTAAGGCGCGGCGGGTCGGTACGATAACTGCGATAAAAGCCATTAATAAGGATAATCCGATGGGGATCGGCAACAACGGCAGGGGCATGTCGATGTAGGCTTTTAAGAC‑GGTTAAGCCGAGTACTTGCGCCCGGAAATAACCGATAATTAAACCGCTGATGAACGCGCATACGGCGATAATTAAGGTTTCCTCGCCGAtttgtt                                                                                                                                                                                                                                        >  1:159033/1‑251 (MQ=255) gTTTTAGCCATGTTATTCCCCTTTTAACACATTGGCGGTTTGAATGTCTAAGGCGCGGCGGGTCGGGACGATAACTGCGATAAAAGCCCTTAATAAGGATAAACCGATGGGGGTCGGCAACACCCGCAGGCGCATGTCGGTGTAGGGTTTGGAGACGGGTTAA‑CCCAGGACCTGCGCCCGTAAATAAACGATAATTAAACCGCTGGTGATCGCGCATACGGCGGTAAATTAGGGTTCCTCGCCGAtttgtt                                                                                                                                                                                                                                        >  1:11922/1‑251 (MQ=255)   tttAGCCATGTTATTCCCCTTTTAACACATTGGCGGTTTGAATGTCTAAGGCGCGGCGGGTCGGTACGATAACTGCGATAAACGCCATTAATAAGGATAATCCGATGGTGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGAtttgtttg                                                                                                                                                                                                                                      <  1:588043/251‑1 (MQ=255)        ccATGTTATTCCCCTTTTAACACATTGGCGGTTTGAATGTCTAAGGCGCGGCGGGTCGGTACGATAACTGCGATAAACGCCATTAATAAGGATAATCCGATGGTGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTtgatga                                                                                                                                                                                                                                  <  1:482525/250‑1 (MQ=255)        ccATGTTATTCCCCTTTTAACACATTGGCGGTTTGAATGTCTAAGGCGCGGCGGGTCGGTACGATAACTGCGATAAACGCCATTAATAAGGATAATCCGATGGTGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTtgatga                                                                                                                                                                                                                                  <  1:472284/250‑1 (MQ=255)          aTGTTATTCCCCTTTTAACACATTGGCGGTTTGAATGTCTAAGGCGCGGCGGGGCGGTACGATAAATGCGATAAAAGCCATTAATAAGGATAATCCGGTGGGGGTCGGCAACACCGGCAGGCGCCTGTCGAAGTAGGCTTTTAAGAC‑GGTTTAACCGAGTACTTGCGCCCGGAAATAACCGATAATTAAGCCGCTGATGATCGCGCCTACGGCGATAATTAAGGGTTCCTCGCCGATTTGTTTGATGATGt                                                                                                                                                                                                                               >  1:689238/1‑251 (MQ=255)                            cacaTTGGCGGTTTGAATGTCTAAGGCGCGGCGGGTCGGTACGATAACTGCGATAAAAGCCCTTAATAAGGATAATCCGATGGGGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGGAAATAAACGATAATTAAACCGCTGATGATCGCGCCTACGGCGATAATTAAGGGTTCCTCGCCGATTTGTTTGATGATGTCCCGGGTTTTTGCGCCgg                                                                                                                                                                                                             >  1:596508/1‑250 (MQ=255)                                      gTTTGAATGTCTAAGGCGCGGCGGGTCGGTACGATAACTGCGATAAACGCCATTAATAAGGATAATCCGATGGTGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGGACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTg                                                                                                                                                                                                   >  1:456566/1‑251 (MQ=255)                                                      cgcgGCGGGTCGGTACGATAACTGCGATAAACGCCATTAATAAGGATAATCCGATGGTGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCtttt                                                                                                                                                                                   <  1:25242/251‑1 (MQ=255)                                                          gcggGTCGGTACGATAACTGCGATAAACGCCATTAATAAGGATAATCCGATGGTGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGcc                                                                                                                                                                                <  1:296809/250‑1 (MQ=255)                                                           cggGTCGGTACGATAACTGCGATAAACGCCATTAATAAGGATAATCCGATGGTGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCg                                                                                                                                                                              <  1:392131/251‑1 (MQ=255)                                                             ggTCGGTACGATAACTGCGATAAACGCCATTAATAAGGATAATCCGATGGTGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGtt                                                                                                                                                                            >  1:577338/1‑251 (MQ=255)                                                                  gTACGATAACTGCGATAAACGCCATTAATAAGGATAATCCGATGGTGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGcc                                                                                                                                                                        >  1:524989/1‑250 (MQ=255)                                                                              cGATAAACGCCATTAATAAGGATAATCCGATGGTGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATg                                                                                                                                                           <  1:899857/251‑1 (MQ=255)                                                                                  aaaCGCCATTAATAAGGATAATCCGATGGTGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGagag                                                                                                                                                       <  1:815354/251‑1 (MQ=255)                                                                                   aaCGCCATTAATAAGGATAATCCGATGGTGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAgt                                                                                                                                                      <  1:391954/251‑1 (MQ=255)                                                                                   aaCGCCATTAATAAGGATAATCCGATGGTGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAgt                                                                                                                                                      <  1:664460/251‑1 (MQ=255)                                                                                        cattaataaGGATAATCCGATGGTGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGgtgt                                                                                                                                                 <  1:828466/248‑1 (MQ=255)                                                                                            aataaGGATAATCCGATGGTGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAgtgg                                                                                                                                                    >  1:70652/1‑244 (MQ=255)                                                                                            aataaGGATAATCCGATGGTGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCa                                                                                                                                              >  1:323729/1‑250 (MQ=255)                                                                                            aataaGGATAATCCGATGGTGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGCACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTtgatga                                                                                                                                                                                                                                  >  1:11935/1‑166 (MQ=255)                                                                                              taaGGATAATCCGATGGTGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGc                                                                                                                                           <  1:256048/251‑1 (MQ=255)                                                                                                 ggATAATCCGATGGTGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTtgatga                                                                                                                                                                                                                                  >  1:360586/1‑161 (MQ=255)                                                                                                 ggATAATCCGATGGTGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGc                                                                                                                                                                                                                         >  1:699652/1‑170 (MQ=255)                                                                                                  gATAATCCGATGGTGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCag                                                                                                                                         <  1:34552/249‑1 (MQ=255)                                                                                                    tAATCCGATGGTGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAggg                                                                                                                                     <  1:399837/251‑1 (MQ=255)                                                                                                    tAATCCGATGGTGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAggg                                                                                                                                     <  1:744638/251‑1 (MQ=255)                                                                                                      aTCCGATGGTGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTg                                                                                                                                   >  1:141199/1‑251 (MQ=255)                                                                                                           aTGGTGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTa                                                                                                                               <  1:731738/250‑1 (MQ=255)                                                                                                              gTGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGc                                                                                                                                           >  1:494791/1‑235 (MQ=255)                                                                                                              gTGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAAGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAggg                                                                                                                                     >  1:427474/1‑241 (MQ=255)                                                                                                                gATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGa                                                                                                                                                             >  1:315991/1‑215 (MQ=255)                                                                                                                 aTCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATATGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCGGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAAttag                                                                                                                        >  1:517909/1‑248 (MQ=255)                                                                                                                             ggCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGc                                                                                                                                 <  1:796771/230‑1 (MQ=255)                                                                                                                                     cATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGtt                                                                                                                                                <  1:419546/207‑1 (MQ=255)                                                                                                                                      aTGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGcc                                                                                                   <  1:101228/251‑1 (MQ=255)                                                                                                                                       tGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGa                                                                                                            >  1:21096/1‑241 (MQ=255)                                                                                                                                           gATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATgag                                                                                                                                                         >  1:561751/1‑192 (MQ=255)                                                                                                                                            aTGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAggg                                                                                                                                     >  1:850458/1‑211 (MQ=255)                                                                                                                                               tAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGcc                                                                                          <  1:641151/251‑1 (MQ=255)                                                                                                                                                  gCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCttt                                                                                       >  1:800947/1‑251 (MQ=255)                                                                                                                                                   cTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTa                                                                                                                               >  1:552615/1‑210 (MQ=255)                                                                                                                                                     ttGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAAt                                                                                    <  1:311191/251‑1 (MQ=255)                                                                                                                                                      tGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATc                                                                                   <  1:329581/251‑1 (MQ=255)                                                                                                                                                            c‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATCTTATcc                                                                             <  1:82870/251‑1 (MQ=255)                                                                                                                                                            c‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTCTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATCTTATcc                                                                             <  1:16967/251‑1 (MQ=255)                                                                                                                                                                         gTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGGTGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATCTTATCCAGAATTTGcc                                                                   >  1:514015/1‑249 (MQ=255)                                                                                                                                                                           aCTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATCTTATCCAGAATTTGCCCtt                                                                >  1:154673/1‑250 (MQ=255)                                                                                                                                                                                     gTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATc                                                                                   >  1:751479/1‑221 (MQ=255)                                                                                                                                                                                     gTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATCTTATCCAGAATTTGCCCTTCGGAGGCGgag                                                     >  1:656902/1‑251 (MQ=255)                                                                                                                                                                                     gTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATCTTATCCAGAATTTGCCCTTCGGAGGCGgag                                                     >  1:355822/1‑251 (MQ=255)                                                                                                                                                                                       aaaTAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATCTTATCCAGAATTTGCCCTTCGGAGGCGgaga                                                    >  1:110398/1‑250 (MQ=255)                                                                                                                                                                                            aCCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATCTTATcc                                                                             <  1:385570/220‑1 (MQ=255)                                                                                                                                                                                            aCCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATCTTATCCAGAATTTGCCCTTCGGAGGCGGAGACTTTg                                               >  1:814636/1‑250 (MQ=255)                                                                                                                                                                                              cGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATc                                                                                   <  1:270322/212‑1 (MQ=255)                                                                                                                                                                                                      aaGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATCTTATCCAGAATTTGCCCTTCGGAGGCGGAGACTTTGCGGATCGGATg                                    <  1:608358/251‑1 (MQ=255)                                                                                                                                                                                                         ccGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATCTTATCCAGAATTTGCCCTTCGGAGGCGGAGACTTTGCGGATCGGATGAat                                 >  1:84185/1‑251 (MQ=255)                                                                                                                                                                                                           gCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTa                                                                                                                               >  1:865653/1‑155 (MQ=255)                                                                                                                                                                                                           gCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATCTTATCCAGAATTTGCCCTTCG‑‑GGCGGAGACTTTGCGGATCGGATGAATATc                              >  1:212420/1‑250 (MQ=255)                                                                                                                                                                                                                    cgcgCATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAgtggtg                                                                                                                                                  >  1:315773/1‑127 (MQ=255)                                                                                                                                                                                                                      cgcATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATc                                                                                   <  1:599162/188‑1 (MQ=255)                                                                                                                                                                                                                      cgcATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATCTTATCCAGAATTTGCCCTTCGGAGGCGGAGACTTTGCGGATCGGATGAATATCCAAATCGGGa                    >  1:101337/1‑251 (MQ=255)                                                                                                                                                                                                                        cATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATCt                                                                                  <  1:755528/187‑1 (MQ=255)                                                                                                                                                                                                                        cATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATCTTATCCAGAATTTGCCCTTCGGAGGCGGAGACTTTGCGGATCGGATGAATATCCAAATCGGGata                  >  1:273287/1‑251 (MQ=255)                                                                                                                                                                                                                        cATACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATCTTATCCAGAATTTGCCCTTCGGAGGCGGAGACTTTGCGGATCGGATGAATATCCAAATCGGGat                   <  1:10847/250‑1 (MQ=255)                                                                                                                                                                                                                          tACGGCGATAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATgag                                                                                                                                                         <  1:583236/114‑1 (MQ=255)                                                                                                                                                                                                                                 aTAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATCTTATCCAGAATTTGCCCTTCGGAGGCGGAGACTTTGCGGATCGGATGAATATCCAAATCGGGATATTGTTGCTg         <  1:38235/251‑1 (MQ=255)                                                                                                                                                                                                                                 aTAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATCTTATCCAGAATTTGCCCTTCGGAGGCGGAGACTTTGCGGATCGGATGAATATCCAAATCGGGATATTGTTGCTg         <  1:407997/251‑1 (MQ=255)                                                                                                                                                                                                                                  tAATTACGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATCTTATCCAGAATTTGCCCTTCGGAGGTGGAGACTTTGCGGATCGGATGAATATCCAAATCGGGATATTGTTGCTGa        >  1:306263/1‑251 (MQ=255)                                                                                                                                                                                                                                  tAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATCTTATCCAGAATTTGCCCTTCGGAGGCGGAGAc                                                   >  1:30370/1‑208 (MQ=255)                                                                                                                                                                                                                                  tAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATCTTATCCAGAATTTGCCCTTCGGAGGCGGAGACTTTGCGGATCGGATGAATATCCAAATCGGGATATTGTTGCTGa        >  1:221708/1‑251 (MQ=255)                                                                                                                                                                                                                                  tAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATCTTATCCAGAATTTGCCCTTCGGAGGCGGAGACTTTGCGGATCGGATGAATATCCAAATCGGGATATTGTTGCTGa        >  1:261592/1‑251 (MQ=255)                                                                                                                                                                                                                                  tAATTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATCTTATCCAGAATTTGCCCTTCGGAGGCGGAGACTTTGCGGATCGGATGAATATCCAAATCGGGATATTGTTGCTGa        >  1:293969/1‑251 (MQ=255)                                                                                                                                                                                                                                   aaTTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATCTTATCCAGAATTTGCCCTTCGGAGGCGGAGACTTTGCGGATCGGATGAATATCCAAATCGGGATAttg               <  1:189280/243‑1 (MQ=255)                                                                                                                                                                                                                                   aaTTAAGGTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATCTTATCCAGAATTTGCCCTTCGGAGGCGGAGACTTTGCGGATCGGATGAATATCCAAATCGGGATATTGTTGCTGaa       >  1:476428/1‑251 (MQ=255)                                                                                                                                                                                                                                          gTTTCCTCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATCTTATCCAGAATTTGCCCTTCGGAGGCGGAGACTTTGCGGATCGGATGAATATCCAAATCGGGATATTGTTGCTGAAGTTGc  <  1:811653/249‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   GTTTTAGCCATGTTATTCCCCTTTTAACACATTGGCGGTTTGAATGTCTAAGGCGCGGCGGGTCGGTACGATAACTGCGATAAACGCCACTAATAAGGATAATCCGATGGTGATCGGCAACACCGGCAGGCGCATGTCGATGTAGGCTTTGAAGAC‑GGTTAAGCCGAGTACTTGCGCCAGTAAATAACCGATAATTAAGCCGCTGATGATCGCGCATACGGCGATAATTAAGGTTTCCGCGCCGATTTGTTTGATGATGTCCCGGCTTTTCGCGCCGAGGGCTTTTTGCAACGCGAATTCTTTTGCCCGTTCGCCCACGATGGCGATGAGAGTGGTGTTCACGCAGAGGGTGGCTAAAATCAGAATCACCACGGAAATCAAGCCCATCAAGCCTTTAATCTTATCCAGAATTTGCCCTTCGGAGGCGGAGACTTTGCGGATCGGATGAATATCCAAATCGGGATATTGTTGCTGAAGTTGC  >  1/653585‑654065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑