Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 651,725 C→T A25T (GCG→ACG)  unknown ← Phosphoglycolate phosphatase (EC 3.1.3.18)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1651,7250CT100.0% 191.0 / NA 71A25T (GCG→ACG) unknownPhosphoglycolate phosphatase (EC 3.1.3.18)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/42);  total (29/42)

TTGCTTGGCGAAATCGATGATTTGCTGAATGTCGGCTCGGAAAATTGAACGGTAATCCACGGTGGCAAAAAGCTGTTTGAAGAAATCAAAATAGCGCTGCCTGATTTCTTCCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGCCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAATATCAATAAGTTAAAAGCACGCGCTATGAAGCGTGCGCTATCAAAGGTGTTTTTTTGTGTTTTCGCGTAGGGGCGGGTCCTGTGCCCG  >  1/651483‑651961
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            
ttGCTTGGCGAAATCGATGATTTGCTGAATGTCGGCTCGGAAAATTGAACGGTAATCCACGGTGGCAAAAAGCTGTTTGAAGAAATCAAAATAGCGCTGCCTGATTTCTTCCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGt                                                                                                                                                                                                                                      <  1:205939/251‑1 (MQ=255)
 tGCTTGGCGAAATCGATGATTTGCTGAATGTCGGCTCGGAAAATTGAACGGTAATCCACGGTGGCAAAAAGCTGTTTGAAGAAATCAAAATAGCGCTGCCTGATTTCTTCCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGt                                                                                                                                                                                                                                      >  1:5741/1‑250 (MQ=255)
      ggCGAAATCGATGATTTGCTGAATGTCGGCTCGGAAAATTGAACGGTAATCCACGGTGGCAAAAAGCTGTTTGAAGAAATCAAAATAGCGCTGCCTGATTTCTTCCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTAtt                                                                                                                                                                                                                                >  1:858902/1‑251 (MQ=255)
                    tttGCTGAATGTCGGCTCGGAAAATTGAACGGTAATCCACGGTGGCAAAAAGCTGTTTGAAGAAATCAAAATAGCGCTGCCTGATTTCTTCCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTa                                                                                                                                                                                                                  >  1:495415/1‑251 (MQ=255)
                     ttGCTGAATGTCGGCTCGGAAAATTGAACGGTAATCCACGGTGGCAAAAAGCTGTTTGAAGAAATCAAAATAGCGCTGCCTGATTTCTTCCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTAt                                                                                                                                                                                                                 <  1:491663/251‑1 (MQ=255)
                           aaTGTCGGCTCGGAAAATTGAACGGTAATCCACGGTGGCAAAAAGCTGTTTGAAGAAATCAAAATAGCGCTGCCTGATTTCTTCCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACa                                                                                                                                                                                                            <  1:247/250‑1 (MQ=255)
                               tCGGCTCGGAAAATTGAACGGTAATCCACGGTGGCAAAAAGCTGTTTGAAGAAATCAAAATAGCGCTGCCTGATTTCTTCCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGa                                                                                                                                                                                                        <  1:688732/250‑1 (MQ=255)
                                     cGGAAAATTGAACGGTAATCCACGGTGGCAAAAAGCTGTTTGAAGAAATCAAAATAGCGCTGCCTGATTTCTTCCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTc                                                                                                                                                                                                 <  1:108046/251‑1 (MQ=255)
                                           aTTGAACGGTAATCCACGGTGGCAAAAAGCTGTTTGAAGAAATCAAAATAGCGCTGCCTGATTTCTTCCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATc                                                                                                                                                                                           <  1:665589/251‑1 (MQ=255)
                                           aTTGAACGGTAATCCACGGTGGCAAAAAGCTGTTTGAAGAAATCAAAATAGCGCTGCCTGATTTCTTCCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATc                                                                                                                                                                                           <  1:368693/251‑1 (MQ=255)
                                           aTTGAACGGTAATCCACGGTGGCAAAAAGCTGTTTGAAGAAATCAAAATAGCGCTGCCTGATTTCTTCCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATc                                                                                                                                                                                           <  1:503057/251‑1 (MQ=255)
                                                   gTAATCCACGGTGGCAAAAAGCTGTTTGAAGAAATCAAAATAGCGCTGCCTGATTTCTTCCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAgatga                                                                                                                                                                                   >  1:177177/1‑251 (MQ=255)
                                                   gTAATCCACGGTGGCAAAAAGCTGTTTGAAGAAATCAAAATAGCGCTGCCTGATTTCTTCCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAgatga                                                                                                                                                                                   >  1:493070/1‑251 (MQ=255)
                                                              tggaaaaaaGCTGTTTGAAGAAATCAAAATAGCGCAGCCTGATTTCATACAAAGAGAGCGAGGAAACGCTTAAGTTTTTCACGATTTCGGGGATTTCCGCCAGTCCATTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTACCTTAATGGATTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAg                                                                                                                                                                         <  1:869581/246‑1 (MQ=255)
                                                               ggCAAAAAGCTGTTTGAAGAAATCAAAATAGCGCTGCCTGATTTCTTCCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCa                                                                                                                                                                       >  1:769514/1‑251 (MQ=255)
                                                                    aaaGCTGTTTGAAGAAATCAAAATAGCGCTGCCTGATTTCTTCCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTTGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATagag                                                                                                                                                                  <  1:428873/251‑1 (MQ=255)
                                                                     aaGCTGTTTGAAGAAATCAAAATAGCGCTGCCTGATTTCTTCCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGt                                                                                                                                                                 <  1:707480/251‑1 (MQ=255)
                                                                            ttGAAGAAATCAAAATAGCGCTGCCTGATTTCTTCCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTa                                                                                                                                                          <  1:102330/251‑1 (MQ=255)
                                                                            ttGAAGAAATCAAAATAGCGCTGCCTGATTTCTTCCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTa                                                                                                                                                          <  1:851986/251‑1 (MQ=255)
                                                                            ttGAAGAAATCAAAATAGCGCTGCCTGATTTCTTCCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTa                                                                                                                                                          <  1:785884/251‑1 (MQ=255)
                                                                                        aaattgcccTGCCTGATTTCTTCCAAAGAGGGCGAGGAACCGCTTAATTTTTTCCCCATTTCGGGGAGTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATAGCTTGTAGCATAGAGTTTCCt                                                                                                                                                            <  1:434741/229‑1 (MQ=255)
                                                                                        aaaTAGCGCTGCCTGATTTCTTCCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATc                                                                                                                                                                                           >  1:780163/1‑206 (MQ=255)
                                                                                          aTAGCGCTGCCTGATTTCTTCCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAAttt                                                                                                                                            <  1:51297/251‑1 (MQ=255)
                                                                                                  gCCTGATTTCTTCCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGAc                                                                                                                                                      >  1:227887/1‑233 (MQ=255)
                                                                                                  gCCTGATTTCTTCCAAAGAGAGCAAGAAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAAAGACGGCGTCCATATCAAAGATGATCGCTTGTAGCATagag                                                                                                                                                                  <  1:706697/221‑1 (MQ=255)
                                                                                                         ttcttcCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACAt                                                                                                                             <  1:35490/251‑1 (MQ=255)
                                                                                                           cttcCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGc                                                                                                                                                                        >  1:689772/1‑206 (MQ=255)
                                                                                                           cttcCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACAttt                                                                                                                           >  1:720578/1‑251 (MQ=255)
                                                                                                           cttcCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACAttt                                                                                                                           >  1:695027/1‑251 (MQ=255)
                                                                                                             tcCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGAc                                                                                                                                                      <  1:111367/222‑1 (MQ=255)
                                                                                                             tcCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTg                                                                                                                         >  1:625112/1‑251 (MQ=255)
                                                                                                             tcCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTg                                                                                                                         >  1:836357/1‑251 (MQ=255)
                                                                                                                       gCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAAttt                                                                                                                                            <  1:749523/222‑1 (MQ=255)
                                                                                                                                  cTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAAcg                                                                                                     <  1:828752/250‑1 (MQ=255)
                                                                                                                                  cTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAAcg                                                                                                     <  1:695258/250‑1 (MQ=255)
                                                                                                                                                 aTTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAATATc                                                                                      <  1:380481/250‑1 (MQ=255)
                                                                                                                                                       gggATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAATATCAATAAg                                                                                <  1:283765/250‑1 (MQ=255)
                                                                                                                                                         gATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGAc                                                                                                                                                      <  1:186637/178‑1 (MQ=255)
                                                                                                                                                             tCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAATATCAATAAGTTAAAAg                                                                         <  1:898868/251‑1 (MQ=255)
                                                                                                                                                              ccGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATagag                                                                                                                                                                  <  1:689891/161‑1 (MQ=255)
                                                                                                                                                               cGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAATATCAATAAGTTAAAAGCa                                                                       >  1:708103/1‑251 (MQ=255)
                                                                                                                                                                    gTCCTTTGCCTATCACTTCCGAACGTTCTAGCGGGGTTAACGAGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAATATCAATAAGTTAAAAGCAcgcg                                                                   >  1:181659/1‑250 (MQ=255)
                                                                                                                                                                         ttGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAata                                                                                        <  1:657025/224‑1 (MQ=255)
                                                                                                                                                                           gCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAATATCAATAAGTTAAAAGc                                                                        >  1:649322/1‑238 (MQ=255)
                                                                                                                                                                            ccTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAATATCAATAAGTTAAAAGCACGCGCTATGAAg                                                           <  1:290672/250‑1 (MQ=255)
                                                                                                                                                                             cTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAATATCAATAAGTTAAAAGCACGCGCTATGAAGCg                                                         <  1:429337/251‑1 (MQ=255)
                                                                                                                                                                              tATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTc                                                                                                           >  1:586450/1‑200 (MQ=255)
                                                                                                                                                                                  aCTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAATATCAATAAGTTAAAAGCACGCGCTATGAAGCGTgcgc                                                    <  1:223842/251‑1 (MQ=255)
                                                                                                                                                                                           cGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAATATCAATAAGTTAAAAGCACGCGCTATGAAg                                                           >  1:425471/1‑235 (MQ=255)
                                                                                                                                                                                                   cGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAATATCAATAAGTTAAAAGCACGCGCTATGAAGCGTGCGCTATCAAAGGTGtttttt                                   <  1:377260/251‑1 (MQ=255)
                                                                                                                                                                                                     gggTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGt                                                                                                                                                                                                                                      >  1:840254/1‑54 (MQ=255)
                                                                                                                                                                                                     gggTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCg                                                                                                                                                                                                                                    <  1:407691/56‑1 (MQ=255)
                                                                                                                                                                                                     gggTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATag                                                                                                                                                                    >  1:714923/1‑120 (MQ=255)
                                                                                                                                                                                                     gggTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAATATCAATAAGTTAAAAGCACGCGCTATGAAGCGTgcgc                                                    <  1:796924/232‑1 (MQ=255)
                                                                                                                                                                                                     gggTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATCGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAATATCAATAAGTTAAAAg                                                                         <  1:435734/211‑1 (MQ=255)
                                                                                                                                                                                                      ggTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAATATCAATAAGTTAAAAGCACGCGCTATGAAGCGTGCGCTATCAAAGGTGTTTTTTtg                                 <  1:144159/250‑1 (MQ=255)
                                                                                                                                                                                                       gTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGAc                                                                                                                                                      <  1:335159/132‑1 (MQ=255)
                                                                                                                                                                                                       gTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAATATCAATAAGTTAAAAGCACGCGCTATGAAg                                                           <  1:392319/223‑1 (MQ=255)
                                                                                                                                                                                                           aCGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAatat                                                                                       <  1:482573/191‑1 (MQ=255)
                                                                                                                                                                                                                cGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAATATCAATAAGTTAAAAGCACGCGCTATGAAGCGTGCGCTATCAAAGGTGTTTTTTTGTGTTTTCGCGt                      <  1:356294/251‑1 (MQ=255)
                                                                                                                                                                                                                  gTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAATATCAATAAGTTAAAAGCACGCGCTATGAAg                                                           >  1:208245/1‑212 (MQ=255)
                                                                                                                                                                                                                  gTCGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAATATCAATAAGTTAAAAGCACGCGCTATGAAGCGTGCGCTATCAAAGGTGTTTTTTTGTGTTTTCGCGTAg                    >  1:608958/1‑251 (MQ=255)
                                                                                                                                                                                                                    cGTGTTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAATATCAATAAGTTAAAAGCACGCGCTATGAAGCGTGCGCTATCAAAGGTGTTTTTTTGTGTTTTCGCGTAggg                  >  1:23576/1‑251 (MQ=255)
                                                                                                                                                                                                                      tgtTCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAATATCAATAAGTTAAAAGCACGCGCTATGAAGCGTGCGCTATCAAAGGTGTTTTTTTGTGTTTTCGCGTAgggg                 <  1:557582/250‑1 (MQ=255)
                                                                                                                                                                                                                         tCCTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAATATCAATAAGTTAAAAGCACGCGCTATGAAGCGTGCGCTATCAAAGGTGTTTTTTtg                                 >  1:433056/1‑231 (MQ=255)
                                                                                                                                                                                                                          ccTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAATATCAATAAGTTAAAAGCACGCGCTATGAAg                                                           >  1:86226/1‑204 (MQ=255)
                                                                                                                                                                                                                          ccTTAATGGCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAATATCAATAAGTTAAAAGCACGCGCTATGAAGCGTGCGCTATCAAAGGTGTTTTTTTGTGTTTTCGCGTAGGGGCgggg            >  1:358358/1‑250 (MQ=255)
                                                                                                                                                                                                                                  gCTTCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAATATCAATAAGTTAAAAGCACGCGCTATGAAGCGTGCGCTATCAAAGGTGTTTTTTTGTGTTTTCGCGTAGGGGCGGGTcc          >  1:314840/1‑245 (MQ=255)
                                                                                                                                                                                                                                     tCAATGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAATATCAATAAGTTAAAAGCACGCGCTATGAAGCGTGCGCTATCAAAGGTGTTTTTTTGTGTTTTCGCGTAGGGGCGGGGCCttggcccg  >  1:650319/1‑243 (MQ=255)
                                                                                                                                                                                                                                        aTGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATagag                                                                                                                                                                  <  1:497295/87‑1 (MQ=255)
                                                                                                                                                                                                                                        aTGAATTGCGTCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAATATCAATAAGTTAAAAGCACGCGCTATGAAGCGTGCGCTATCAAAGGTGTTTTTTTGTGTTTTCGCGTAGGGGCGGGTCCTGTgc     >  1:493584/1‑244 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            
TTGCTTGGCGAAATCGATGATTTGCTGAATGTCGGCTCGGAAAATTGAACGGTAATCCACGGTGGCAAAAAGCTGTTTGAAGAAATCAAAATAGCGCTGCCTGATTTCTTCCAAAGAGAGCGAGGAACCGCTTAATTTTTTCACGATTTCGGGGATTTCCGCCAGTCCTTTGCCTATCACTTCCGAACGTTCGAGCGGGGTTAACGTGCGGTCGTGTTCCTTAATGGCTTCAATGAATTGCGCCTGTAAGTCGTATTCTAAAAATTCGGTATCGACAATGACGCCGTCCATATCAAAGATGATCGCTTGTAGCATAGAGTTTCCTTATGACAGTTGAATTTTGAAAAACGTTACATTTTGCTGGTGCGCGTTTCCTAACGCGTGCCTGTAATATCAATAAGTTAAAAGCACGCGCTATGAAGCGTGCGCTATCAAAGGTGTTTTTTTGTGTTTTCGCGTAGGGGCGGGTCCTGTGCCCG  >  1/651483‑651961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑