Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	649,813	A→G	S8G (AGC→GGC)	unknown →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	649,813	0	A	G	100.0%	255.0 / NA	87	S8G (AGC→GGC)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (49/38);  total (49/38)

TATTATAAGTTTTACTAAGGAGAATTTTACAAATAATAGAGGCTATACTTTGGAATTGAATATATCAATTAAATTAGAATAATATTATCCCCCCACGATGGCGAGCGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAAGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGACCTTTACCGGATTATTTGATAAACCGATAAAGCATGCAAAATATAATTCGATTAGCGACCGATTAAGAACCATTAATGCA  >  1/649613‑650058                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      tattataAGTTTTACTAAGGAGAATTTTACAAATAATAGAGGCTATACTTTGGAATTGAATATATCAATTAAATTAGAATAATATTATCCCCCCACGATGGCGAGCGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGtt                                                                                                                                                                                                     >  1:848504/1‑251 (MQ=255)                                 ataataGAGGCTATACTTTGGAATTGAATATATCAATTAAATTAGAATAATATTATCCCCCCACGATGGCGAGCGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGt                                                                                                                                                                     >  1:463899/1‑251 (MQ=255)                                  taataGAGGCTATACTTTGGAATTGAATATATCAATTAAATTAGAATAATATTATCCCCCCACGATGGCGAGCGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTg                                                                                                                                                                    >  1:376455/1‑251 (MQ=255)                                    ataGAGGCTATACTTTGGAATTGAATATATCAATTAAATTAGAATAATATTATCCCCCCACGATGGCGAGCGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTc                                                                                                                                                                                                                                              <  1:158951/175‑1 (MQ=255)                                        agGCTATACTTTGGAATTGAATATATCAATTAAATTAGAATAATATTATCCCCCCACGATGGCGAGCGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTGTTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGttt                                                                                                                                                              >  1:223762/1‑251 (MQ=255)                                        agGCTATACTTTGGAATTGAATATATCAATTAAATTAGAATAATATTATCCCCCCACGATGGCGAGCGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGttt                                                                                                                                                              >  1:690560/1‑251 (MQ=255)                                         ggCTATACTTTGGAATTGAATATATCAATTAAATTAGAATAATATTATCCCCCCACGATGGCGAGCGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAAc                                                                                                                                                                                                                                     >  1:372063/1‑179 (MQ=255)                                         ggCTATACTTTGGAATTGAATATATCAATTAAATTAGAATAATATTATCCCCCCACGATGGCGAGCGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCa                                                                                                                                                                                                         >  1:304780/1‑207 (MQ=255)                                                          gAATATATCAATTAAATTAGAATAATATTATCCCCCCACGATGGCGAGCGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTtttattt                                                                                                                                                         >  1:311729/1‑238 (MQ=255)                                                          gAATATATCAATTAAATTAGAATAATATTATCCCCCCACGATGGCGAGCGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTtca                                                                                                                                            >  1:712635/1‑251 (MQ=255)                                                                        aattaGAATAATATTATCCCCCCACGATGGCGAGCGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATg                                                                                                                                                                                                                          <  1:290822/159‑1 (MQ=255)                                                                             gAATAATATTATCCCCCCACGATGGCGAGCGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTatat                                                                                                                                                                                                                                                <  1:771873/132‑1 (MQ=255)                                                                             gAATAATATTATCCCCCCACGATGGCGAGCGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTa                                                                                                                                                                                                                                <  1:198811/148‑1 (MQ=255)                                                                             gAATAATATTATCCCCCCACGATGGCGAGCGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTtttattt                                                                                                                                                         <  1:530891/219‑1 (MQ=255)                                                                             gAATAATATTATCCCCCCACGATGGCGAGCGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTtc                                                                                                                                             <  1:173274/231‑1 (MQ=255)                                                                             gAATAATATTATCCCCCCACGATGGCGAGCGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGAc                                                                                                                            <  1:263493/248‑1 (MQ=255)                                                                             gAATAATATTATCCCCCCACGATGGCGAGCGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATa                                                                                                                         >  1:398931/1‑251 (MQ=255)                                                                             gAATAATATTATCCCCCCACGATGGCGAGCGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATa                                                                                                                         >  1:426036/1‑251 (MQ=255)                                                                                  atatTATCCCCCCACGATGGCGAGCGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCa                                                                                                                                                                                                         >  1:317036/1‑166 (MQ=255)                                                                                  atatTATCCCCCCACGATGGCGAGCGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAAc                                                                                                                       <  1:508963/248‑1 (MQ=255)                                                                                  atatTATCCCCCCACGATGGCGAGCGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAg                                                                                                                     >  1:475602/1‑250 (MQ=255)                                                                                  atatTATCCCCCCACGATGGCGAGCGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAg                                                                                                                     >  1:321399/1‑250 (MQ=255)                                                                                  atatTATCCCCCCACGATGGCGAGCGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGc                                                                                                                    <  1:220290/251‑1 (MQ=255)                                                                                         ccccccACGATGGCGAGCGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGaa                                                                                                             <  1:472364/251‑1 (MQ=255)                                                                                           gatatttcaggcacacgttaggaaaCCACAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGc                                                                                                                    <  1:311509/218‑1 (MQ=255)                                                                                           ccccACGATGGCGAGCGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACa                                                                                                           >  1:415516/1‑251 (MQ=255)                                                                                             ccACGATGGCGAGCGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAGCAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATg                                                                                                                                  >  1:523847/1‑226 (MQ=255)                                                                                               aCGATGGCGAGCGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGCAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGAc                                                                                                                            >  1:429306/1‑230 (MQ=255)                                                                                                          cGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTAc                                                                                            <  1:273014/251‑1 (MQ=255)                                                                                                          cGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTAc                                                                                            <  1:854388/251‑1 (MQ=255)                                                                                                            tctcCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAAc                                                                                                                                                                                                                                     >  1:36925/1‑112 (MQ=255)                                                                                                            tctcCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTtc                                                                                                                                             >  1:463044/1‑200 (MQ=255)                                                                                                            tctcCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAg                                                                                          >  1:120592/1‑251 (MQ=255)                                                                                                            tctcCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAg                                                                                          >  1:359352/1‑251 (MQ=255)                                                                                                            tctcCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAg                                                                                          >  1:292869/1‑251 (MQ=255)                                                                                                             ctcCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCTCCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCta                                                                                                                                                                                                                                                   <  1:398575/97‑1 (MQ=255)                                                                                                             ctcCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGc                                                                                                                                                                             >  1:412498/1‑167 (MQ=255)                                                                                                             ctcCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGt                                                                                         >  1:636680/1‑251 (MQ=255)                                                                                                                  gACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGa                                                                                                                                                                   <  1:836525/172‑1 (MQ=255)                                                                                                                  gACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGc                                                                                                    >  1:785741/1‑235 (MQ=255)                                                                                                                  gACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTtctc                                                                                     >  1:683293/1‑250 (MQ=255)                                                                                                                   aCCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCg                                                                                   >  1:94782/1‑251 (MQ=255)                                                                                                                    ccATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGa                                                                                  >  1:379480/1‑251 (MQ=255)                                                                                                                       tAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTtttat                                                                                                                                                           <  1:792424/175‑1 (MQ=255)                                                                                                                          cTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGAcc                                                                                <  1:796598/247‑1 (MQ=255)                                                                                                                            gTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGAc                                                                                                                            >  1:403995/1‑201 (MQ=255)                                                                                                                                    ccTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGACCTTTACCGGattatt                                                                  >  1:9174/1‑251 (MQ=255)                                                                                                                                       gagaAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCt                                                                                                                                                                                                                                                    <  1:806948/70‑1 (MQ=255)                                                                                                                                              aGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTtttattt                                                                                                                                                         >  1:255196/1‑154 (MQ=255)                                                                                                                                               ggTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAAc                                                                                                            <  1:620203/198‑1 (MQ=255)                                                                                                                                                    aaTCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGACCTTTACCGGATTATTTGATAAACCGATAAAg                                                  <  1:70764/251‑1 (MQ=255)                                                                                                                                                    aaTCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGACCTTTACCGGATTATTTGATAAACCGATAAAg                                                  <  1:887318/251‑1 (MQ=255)                                                                                                                                                       cACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGACCTTTACCGGat                                                                      >  1:791549/1‑228 (MQ=255)                                                                                                                                                         ccTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATg                                                                                                                                  <  1:533988/166‑1 (MQ=255)                                                                                                                                                            tattaACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTc                                                                                                                                                                                                                                              <  1:128815/55‑1 (MQ=255)                                                                                                                                                            tattaACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTtttattt                                                                                                                                                         <  1:26621/140‑1 (MQ=255)                                                                                                                                                            tattaACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTAc                                                                                                                                                       >  1:793503/1‑142 (MQ=255)                                                                                                                                                            tattaACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAg                                                                                                                     <  1:291568/176‑1 (MQ=255)                                                                                                                                                            tattaACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAg                                                                                          >  1:356233/1‑203 (MQ=255)                                                                                                                                                            tattaACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAg                                                                                          <  1:327073/203‑1 (MQ=255)                                                                                                                                                            tattaACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGACCTTTACCGGATTATTTGATAAACCGATAAAg                                                  >  1:86967/1‑243 (MQ=255)                                                                                                                                                             attaACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGACCTTTACCGGATTATTTGATAAACCGATAAAGCATGCaaaa                                         >  1:773516/1‑251 (MQ=255)                                                                                                                                                             attaACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGACCTTTACCGGATTATTTATTAACCCGATAAAGCATGCac                                           >  1:305104/1‑248 (MQ=255)                                                                                                                                                                aaCAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAg                                                                                                                     >  1:224215/1‑172 (MQ=255)                                                                                                                                                                aaCAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACAtt                                                                                                         >  1:161703/1‑184 (MQ=255)                                                                                                                                                                aaCAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGACCTTTACCGGATTATTTGATAAACCGATAAAGCATGCAAAatat                                      <  1:691305/251‑1 (MQ=255)                                                                                                                                                                 aCAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGACCTTTACCGGATTATTTGATAAACCGATAAAGCATGCAAAAtata                                     >  1:483144/1‑251 (MQ=255)                                                                                                                                                                    gagagTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGACCTTTACCGGATTATTTGATAAACCGATAAAGCATGCaaa                                          <  1:317832/243‑1 (MQ=255)                                                                                                                                                                     agagTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGACCTTTACCGGATTATTTGATAAACCGATAAAGCATGCAAAATATaa                                    <  1:612973/248‑1 (MQ=255)                                                                                                                                                                     agagTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGACCTTTACCGGATTATTTGATAAACCGATAAAGCATGCAAAATATAAtt                                  >  1:461323/1‑250 (MQ=255)                                                                                                                                                                     agagTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGACCTTTACCGGATTATTTGATAAACCGATAAAGCATGCAAAATATAAtt                                  <  1:270973/250‑1 (MQ=255)                                                                                                                                                                      gagTGAGGTGAGCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATg                                                                                                                                  >  1:256563/1‑153 (MQ=255)                                                                                                                                                                          gaggtgagCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGACCTTTACCGGATTATTTGATAAACCGATAAAGCATGCAAAATATAATTCGATTa                            >  1:661033/1‑251 (MQ=255)                                                                                                                                                                            ggtgagCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGt                                                                                                                                                                                                      <  1:409676/79‑1 (MQ=255)                                                                                                                                                                               gagCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGACCTTTACCGGATTATTTGATAAACCGATAAAGCATGCAAAATATAATTCGATTAGCGa                        >  1:463183/1‑250 (MQ=255)                                                                                                                                                                                 gCGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGCGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGACCTTTACCGGATTATTTGATAAACCGATAAAGCATGCAAAATATAATTCGATTAGCGACCg                     >  1:103410/1‑251 (MQ=255)                                                                                                                                                                                  cGATGAAACTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAg                                                                                                                     >  1:818447/1‑154 (MQ=255)                                                                                                                                                                                       aaaCTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGACCTTTACCGGATTATTTGATAAACCGATAAAGCATGCAAAATATAATTCGATTAGCGACCGATTaa                <  1:454174/250‑1 (MQ=255)                                                                                                                                                                                          cTCAGAGAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGACCTTTACCGGATTATTTGATAAACCGATAAAGCATGCAAAATATAATTCGATTAGCGACCGATTAAGAAc            <  1:397592/251‑1 (MQ=255)                                                                                                                                                                                             agagAAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGACCTTTACCGGATTATTTGATAAACCGATAAAGCATGCAAAATATAATTCGATTAGCGACCGATTAAGAACCAt         >  1:636712/1‑251 (MQ=255)                                                                                                                                                                                              gagaAATCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGACCTTTACCGGATTATTTGATAAACCGATAAAg                                                  <  1:698003/209‑1 (MQ=255)                                                                                                                                                                                                   aTCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGACCTTTACCGGATTATTTg                                                                >  1:874901/1‑190 (MQ=255)                                                                                                                                                                                                   aTCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGACCTTTACCGGATTATTTGATAAACCGATAAAGCATGCAAAATATAATTCGATTAGCGACCGATTAAGAAc            <  1:122037/242‑1 (MQ=255)                                                                                                                                                                                                   aTCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGACCTTTACCGGATTATTTGATAAACCGATAAAGCATGCAAAATATAATTCGATTAGCGACCGATTAAGAACCATTAATGc   <  1:771210/251‑1 (MQ=255)                                                                                                                                                                                                   aTCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGACCTTTACCGGATTATTTGATAAACCGATAAAGCATGCAAAATATAATTCGATTAGCGACCGATTAAGAACCATTAATGc   <  1:875345/251‑1 (MQ=255)                                                                                                                                                                                                   aTCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGACCTTTACCGGATTATTTGATAAACCGATAAAGCATGCAAAATATAATTCGATTAGCGACCGATTAAGAACCATTAATGc   <  1:392427/251‑1 (MQ=255)                                                                                                                                                                                                    tCTTAGGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGACCTTTACCGGATTATTTGATAAACCGATAAAGCATGCAAAATATAATTCGATTAGCGACCGATTAAGAACCATTAATGCa  >  1:41366/1‑251 (MQ=255)                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      TATTATAAGTTTTACTAAGGAGAATTTTACAAATAATAGAGGCTATACTTTGGAATTGAATATATCAATTAAATTAGAATAATATTATCCCCCCACGATGGCGAGCGTCTCCCGACCATAGCTGTTCGAAACCTGAGAAAAAGGTAAAATCACCTTATTAACAGAGAGTGAGGTGAGCGATGAAACTCAGAGAAATCTTAAGCTATATTCCGAAAGAACAGTTAAGAATGTTTGCATTGGAATATCAAGTTGACCGTCAAGTGAAAAAATTAAGCGGTGAAGTGATGTTTTATTTACTGCTATTTTCATCATTAAATGTACGACATAACAGCTTAAGAACATTAGAGCAATTTTACAGTTCTCCGACCTTTACCGGATTATTTGATAAACCGATAAAGCATGCAAAATATAATTCGATTAGCGACCGATTAAGAACCATTAATGCA  >  1/649613‑650058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑