Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	649,383	T→C	*334Q (TAA→CAA)	unknown →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	649,383	0	T	C	100.0%	33.3 / NA	13	*334Q (TAA→CAA)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/9);  total (4/9)

AATCTACCAAATAAGAGATAAAGAAGATTTAGAAAGAAGTGTATGTAACATAAAGAAATTAAAAGGTGTAGAATCTAATCCTCCTTTTGTTCCTTCAAAGAAAGGCTGGTCTCCATATTTGGCTAGAAATTTTCTTAAAAATGACAAAATTATAATGACTGACTATCACCCTGGCTATGATAATAGATGGTGGGCGTATAGTAGGCCATTACTAATAGATTAAGAAAATTCATTTTCAGATATGATTACATATTATATATATGGAGATGAAAAAATTGTTGGTAATTTAAAATTAAAAGTTTATATTAATAATGTATCCATATCGGAATCTACAAAAGAAGTTATCTTAAAGGTATTTAGTGGTTTATTGCTTAATTCAATAAAGTGTGATTCTATTAAAA  >  1/649163‑649563                                                                                                                                                                                                                             |                                                                                                                                                                                     aaTCTACCAAATAAGAGATAAAGAAGATTTAGAAAGAAGTGTATGTAACATAAAGAAATTAAAAGGTGTAGAATCTAATCCTCCTTTTGTTCCTTCAAAGAAAGGCTGGTCTCCATATTTGGCTAGAAATTTTCTTAAAAATGACAAAATTATAATGACTGACTATCACCCTGGCTATGATAATAGATGGTGGGCGTATAGTAGGCCATTACTAATAGATCAAGAAAATTCATTTTCAGATATGATTACat                                                                                                                                                        <  1:718000/251‑1 (MQ=255)                                                          aTTAAAAGGTGTAGAATCTAATCCTCCTTTTGTTCCTTCAAAGAAAGGCTGGTCTCCATATTTGGCTAGAAATTTTCTTAAAAATGACAAAATTATAATGACTGACTATCACCCTGGCTATGATAATAGATGGTGGGCGTATAGTAGGCCATTACTAATAGATCAAGAAAATTCATTTTCAGat                                                                                                                                                                  >  1:626064/1‑184 (MQ=255)                                                                  gtgtAGAATCTAATCCTCCTTTTGTTCCTTCAAAGAAAGGCTGGTCTCCATATTTGGCTAGAAATTTTCTTAAAAATGACAAAATTATAATGACTGACTATCACCCTGGCTATGATAATAGATGGTGGGCGTATAGTAGGCCATTACTAATAGATCAAGAAAATTCATTTTCAGATATGATTACATATtatat                                                                                                                                                 <  1:530078/193‑1 (MQ=255)                                                                  gtgtAGAATCTAATCCTCCTTTTGTTCCTTCAAAGAAAGGCTGGTCTCCATATTTGGCTAGAAATTTTCTTAAAAATGACAAAATTATAATGACTGACTATCACCCTGGCTATGATAATAGATGGTGGGCGTATAGTAGGCCATTACTAATAGATCAAGAAAATTCATTTTCAGATATGATTACATATTATATATATGGAGATGAAAAAATTGTTGGTAATTTAAAATTAAAAGTTTATATTAATAATg                                                                                         >  1:606323/1‑249 (MQ=255)                                                                                   cctTTTGTTCCTTCAAAGAAAGGCTGGTCTCCATATTTGGCTAGAAATTTTCTTAAAAATGACAAAATTATAATGACTGACTATCACCCTGGCTATGATAATAGATGGTGGGCGTATAGTAGGCCATTACTAATAGATCAAGAAAATTCATTTTCAGATATGAt                                                                                                                                                             >  1:249531/1‑164 (MQ=255)                                                                                                             gTCTCCATATTTGGCTAGAAATTTTCTTAAAAATGACAAAATTATAATGACTGACTATCACCCTGGCTATGATAATAGATGGTGGGCGTATAGTAGGCCATTACTAATAGATCAAGAAAATTCATTTTCAGATATGAt                                                                                                                                                             <  1:410472/138‑1 (MQ=255)                                                                                                             gTCTCCATATTTGGCTAGAAATTTTCTTAAAAATGACAAAATTATAATGACTGACTATCACCCTGGCTATGATAATAGATGGTGGGCGTATAGTAGGCCATTACTAATAGATCAAGAAAATTCATTTTCAGATATGATTACATATtatata                                                                                                                                                >  1:381103/1‑151 (MQ=255)                                                                                                             gTCTCCATATTTGGCTAGAAATTTTCTTAAAAATGACAAAATTATAATGACTGACTATCACCCTGGCTATGATAATAGATGGTGGGCGTATAGTAGGCCATTACTAATAGATCAAGAAAATTCATTTTCAGATATGATTACATATTatatatat                                                                                                                                             <  1:478575/154‑1 (MQ=255)                                                                                                               ctcCATATTTGGCTAGAAATTTTCTTAAAAATGACAAAATTATAATGACTGACTATCACCCTGGCTATGATAATAGATGGTGGGCGTATAGTAGGCCATTACTAATAGATCAAGAAAATTCATTTTCAGATATGATTACatat                                                                                                                                                      <  1:816409/143‑1 (MQ=255)                                                                                                                                                       tataATGACTGACTATCACCCTGGCTATGATAATAGATGGTGGGCGTATAGTAGGCCATTACTAATAGATCAAGAAAATTCATTTTCAGATATGATTACATATTATATATATGGAGATGAAAAAATTGTTGGTAATTTAAAATTAAAAGTTTATATTAATAATGTATCCATATCGGAATCTACAAAAGAAGTTATCTTAAAGGTATTTAGTGGTTTATTGCTTAATTCAATAAAGTGTGATTCTATTaaaa  <  1:279661/251‑1 (MQ=255)                                                                                                                                                                                 aTGATAATAGATGGTGGGCGTATAGTAGGCCATTACTAATAGATCAAGAAAATTCATTTTCAGATATGATTACATATTATATATATGGAGATGAAAAAATTGTTGGTAATTTAAAATTAAAAGTTTATATTAATAATGt                                                                                        <  1:121143/139‑1 (MQ=255)                                                                                                                                                                                                      ataGTAGGCCATTACTAATAGATCAAGAAAATTCATTTTCAGATATGATTACATATTATATATATGGAGATGAAAAAATTGTTGGTAATTTAAAATTAAAAGTTTATATTAATAATGTATcc                                                                                    <  1:209049/122‑1 (MQ=255)                                                                                                                                                                                                                     tAATAGATCAAGAAAATTCATTTTCAGATATGATTACATATTATATATATGGAGATGAAAAAATTGTTGGTAATTTAAAATTAAAAGTTTATATTAATAATGTATCCATATCGGAATCTACAAAAGAAGTTATCTTAAAGGTATTTAGTGGTTTATTGCTTAATTCAATaa                    <  1:654399/171‑1 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                     AATCTACCAAATAAGAGATAAAGAAGATTTAGAAAGAAGTGTATGTAACATAAAGAAATTAAAAGGTGTAGAATCTAATCCTCCTTTTGTTCCTTCAAAGAAAGGCTGGTCTCCATATTTGGCTAGAAATTTTCTTAAAAATGACAAAATTATAATGACTGACTATCACCCTGGCTATGATAATAGATGGTGGGCGTATAGTAGGCCATTACTAATAGATTAAGAAAATTCATTTTCAGATATGATTACATATTATATATATGGAGATGAAAAAATTGTTGGTAATTTAAAATTAAAAGTTTATATTAATAATGTATCCATATCGGAATCTACAAAAGAAGTTATCTTAAAGGTATTTAGTGGTTTATTGCTTAATTCAATAAAGTGTGATTCTATTAAAA  >  1/649163‑649563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑