Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	646,212	G→A	R643H (CGC→CAC)	unknown →	ATP‑dependent helicase HrpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	646,212	0	G	A	100.0%	175.0 / NA	65	R643H (CGC→CAC)	unknown	ATP‑dependent helicase HrpA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/36);  total (29/36)

GTCAATTTTTGCAGCGTTTATGAAGTAATGATCATCGTTTCGGCGTTATCCATTCAGGATCTGCGCGAACGTCCGACGGAAAAACAACAGGCAGCGGACGAAAAACATCGTCGTTTTGCCGATAAAAAATCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCGCGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCATATCGGGCTGAAAGAAGCGGAAAAACAACAATATCTCGGCGCGCGCAACGCCCATTTCGCCATTTTCCCCAATTCCGTGCTTTTCAAAAAACAACCGAAATGGGTAATGGCGGCGGAG  >  1/645965‑646453                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  gTCAATTTTTGCAGCGTTTATGAAGTAATGATCATCGTTTCGGCGTTATCCATTCAGGATCTGCGCGAACGTCCGACGGAAAAACAACAGGCAGCGGACGAAAAACATCGTCGTTTTGCCGATAAAAAATCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGa                                                                                                                                                                                                                                                >  1:597242/1‑251 (MQ=255)                gTTTATGAAGTAATGATCATCGTTTCGGCGTTATCCATTCAGGATCTGCGCGAACGTCCGACGGAAAAACAACAGGCAGCGGACGAAAAACATCGTCGTTTTGCCGATAAAAAATCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTa                                                                                                                                                                                                                                 >  1:279236/1‑251 (MQ=255)                 tttATGAAGTAATGATCATCGTTTCGGCGTTATCCATTCAGGATCTGCGCGAACGTCCGACGGAAAAACAACAGGCAGCGGACGAAAAACATCGTCGTTTTGCCGATAAAAAATCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTa                                                                                                                                                                                                                                 <  1:48545/250‑1 (MQ=255)                 tttATGAAGTAATGATCATCGTTTCGGCGTTATCCATTCAGGATCTGCGCGAACGTCCGACGGAAAAACAACAGGCAGCGGACGAAAAACATCGTCGTTTTGCCGATAAAAAATCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCACTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTAt                                                                                                                                                                                                                                >  1:425947/1‑251 (MQ=255)                  ttATGAAGTAATGATCATCGTTTCGGCGTTATCCATTCAGGATCTGCGCGAACGTCCGACGGAAAAACAACAGGCAGCGGACGAAAAACATCGTCGTTTTGCCGATAAAAAATCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATc                                                                                                                                                                                                                               <  1:77948/251‑1 (MQ=255)                  ttATGAAGGAATGAACATCGTTTCGGCGTTATCCATTCAGGATCTGCGCGAACGTCCGACGGAAAAACAACAGGCAGCGGACGAAAAACATCGTCGTTTTGCCGATAAAAAATCGGATTTCTTGGCGTTCCTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATc                                                                                                                                                                                                                               <  1:44702/251‑1 (MQ=255)                    aTGAAGTAATGATCATCGTTTCGGCGTTATCCATTCAGGATCTGCGCGAACGTCCGACGGAAAAACAACAGGCAGCGGACGAAAAACATCGTCGTTTTGCCGATAAAAAATCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAg                                                                                                                                                                                                                             >  1:855495/1‑251 (MQ=255)                         gTAATGATCATCGTTTCGGCGTTATCCATTCAGGATCTGCGCGAACGTCCGACGGAAAAACAACAGGCAGCGGACGAAAAACATCGTCGTTTTGCCGATAAAAAATCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATc                                                                                                                                                                                                                               <  1:678410/244‑1 (MQ=255)                         gTAATGATCATCGTTTCGGCGTTATCCATTCAGGATCTGCGCGAACGTCCGACGGAAAAACAACAGGCAGCGGACGAAAAACATCGTCGTTTTGCCGATAAAAAATCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAAt                                                                                                                                                                                                                        >  1:616788/1‑251 (MQ=255)                           aaTGATCATCGTTTCGGCGTTATCCATTCAGGATCTGCGCGAACGTCCGACGGAAAAACAACAGGCAGCGGACGAAAAACATCGTCGTTTTGCCGATAAAAAATCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTAAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAAtt                                                                                                                                                                                                                       >  1:380471/1‑250 (MQ=255)                                    cGTTTCGGCGTTATCCATTCAGGATCTGCGCGAACGTCCGACGGAAAAACAACAGGCAGCGGACGAAAAACATCGTCGTTTTGCCGATAAAAAATCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCgg                                                                                                                                                                                                             <  1:736797/251‑1 (MQ=255)                                    cGTTTCGGCGTTATCCATTCAGGATCTGCGCGAACGTCCGACGGAAAAACAACAGGCAGCGGACGAAAAACATCGTCGTTTTGCCGATAAAAAATCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCgg                                                                                                                                                                                                             <  1:150308/251‑1 (MQ=255)                                      tttCGGCGTTATCCATTCAGGATCTGCGCGAACGTCCGACGGAAAAACAACAGGCAGCGGACGAAAAACATCGTCGTTTTGCCGATAAAAAATCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTg                                                                                                                                                                                                           >  1:479653/1‑251 (MQ=255)                                                     ttCAGGATCTGCGCGAACGTCCGACGGAAAAACAACAGGCAGCGGACGAAAAACATCGTCGTTTTGCCGATAAAAAATCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTa                                                                                                                                                                                                                                 >  1:24390/1‑214 (MQ=255)                                                     ttCAGGATCTGCGCGAACGTCCGACGGAAAAACAACAGGCAGCGGACGAAAAACATCGTCGTTTTGCCGATAAAAAATCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATc                                                                                                                                                                                                                               <  1:584597/216‑1 (MQ=255)                                                     ttCAGGATCTGCGCGAACGTCCGACGGAAAAACAACAGGCAGCGGACGAAAAACATCGTCGTTTTGCCGATAAAAAATCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGc                                                                                                                                                                                                                            <  1:26856/219‑1 (MQ=255)                                                       cAGGATCTGCGCGAACGTCCGACGGAAAAACAACAGGCAGCGGACGAAAAACATCGTCGTTTTGCCGATAAAAAATCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGatat                                                                                                                                                                                                                                    >  1:890743/1‑209 (MQ=255)                                                            tCTGCGCGAACGTCCGACGGAAAAACAACAGGCAGCGGACGAAAAACATCGTCGTTTTGCCGATAAAAAATCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTa                                                                                                                                                                                     <  1:465509/251‑1 (MQ=255)                                                                        tCCGACGGAAAAACAACAGGCAGCGGACGAAAAACATCGTCGTTTTGCCGATAAAAAATCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGCGAAATGGGcgttgcgcgcgccgagatattgt                                                                                                                                                                         <  1:29559/251‑23 (MQ=255)                                                                               gAAAAACAACAGGCAGCGGACGAAAAACATCGTCGTTTTGCCGATAAAAAATCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAata                                                                                                                                                                  >  1:76697/1‑251 (MQ=255)                                                                                aaaaaCAACAGGCAGCGGACGAAAAACATCGTCGTTTTGCCGATAAAAAATCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAatat                                                                                                                                                                 <  1:34312/251‑1 (MQ=255)                                                                                    acaacaGGCAGCGGACGAAAAACATCGTCGTTTTGCCGATAAAAAATCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATc                                                                                                                                                                                                                               >  1:499898/1‑185 (MQ=255)                                                                                     caacaGGCAGCGGACGAAAAACATCGTCGTTTTGTCGATAAAAAATCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCa                                                                                                                                                            <  1:403476/251‑1 (MQ=255)                                                                                                 gACGAAAAACATCGTCGTTTTGCCGATAAAAAATCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCg                                                                                                                                                 >  1:733523/1‑250 (MQ=255)                                                                                                           aTCGTCGTTTTGCCGATAAAAAATCGGATTTCTTGGGGTTCTTGAAACTGTGGGAATGA‑CTGCAAGAACAACAAAAAGCGCTGGGGAAAAACCAATTCCGTCGGCAATGGCAAAAAGGTTTCCTCAAATACCTGCGCATTCACGAATGGGAGGATATTTAACAGCAAATTCGTCTGGCGGTGGGTGGAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGt                                                                                                                                      <  1:11841/251‑1 (MQ=255)                                                                                                           aTCGTCGTTTTGCCGATAAAAAATCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGt                                                                                                                                      <  1:87326/251‑1 (MQ=255)                                                                                                           aTCGTCGTTTTGCCGATAAAAAATCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGt                                                                                                                                      <  1:537604/251‑1 (MQ=255)                                                                                                                            aaaaaaTCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCTGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATGCCGCACTTTTAAGTGGCTTGTTGTCCCatat                                                                                                                     <  1:435308/251‑1 (MQ=255)                                                                                                                            aaaaaaTCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCatat                                                                                                                     <  1:178757/251‑1 (MQ=255)                                                                                                                            aaaaaaTCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCatat                                                                                                                     <  1:622647/251‑1 (MQ=255)                                                                                                                                                 ttCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCatat                                                                                                                     >  1:396291/1‑230 (MQ=255)                                                                                                                                                 ttCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCATATCGGGCTGAAAGAAGCGGaaaa                                                                                                >  1:454240/1‑251 (MQ=255)                                                                                                                                                  tCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATAc                                                                                                                                                   <  1:104157/199‑1 (MQ=255)                                                                                                                                                  tCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAg                                                                                                                                       <  1:480252/211‑1 (MQ=255)                                                                                                                                                  tCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCATATCGGGCTGAAAGAAGCGGaaaaa                                                                                               >  1:473093/1‑251 (MQ=255)                                                                                                                                                   cTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCATATCGGGCTGAAAGAAGCGGAAAaac                                                                                              <  1:503988/251‑1 (MQ=255)                                                                                                                                                       aaCCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCATATCGGGCTGAAAGAAGCGGAAAaacaac                                                                                           <  1:256480/250‑1 (MQ=255)                                                                                                                                                          cTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAAt                                                                                                                                                                                                   <  1:50999/143‑1 (MQ=255)                                                                                                                                                          cTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCATATCGGGCTGAAAGAAGCGGAAAAACAACAata                                                                                       >  1:496784/1‑251 (MQ=255)                                                                                                                                                           tgt‑gGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCATATCGGGCTGAAAGAAGCGGAAAAACAACAatat                                                                                      <  1:96550/251‑1 (MQ=255)                                                                                                                                                           tgt‑gGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCATATCGGGCTGAAAGAAGCGGAAAAACAACAatat                                                                                      <  1:691084/251‑1 (MQ=255)                                                                                                                                                           tgt‑gGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCATATCGGGCTGAAAGAAGCGGAAAAACAACAatat                                                                                      <  1:834739/251‑1 (MQ=255)                                                                                                                                                             t‑gGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCATATCGGGCTGAAAGAAGCGGAAAAACAACAATAtc                                                                                     <  1:731997/250‑1 (MQ=255)                                                                                                                                                             t‑gGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCATATCGGGCTGAAAGAAGCGGAAAAACAACAATAtc                                                                                     <  1:568986/250‑1 (MQ=255)                                                                                                                                                                       cTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGt                                                                                                                                      >  1:892560/1‑192 (MQ=255)                                                                                                                                                                             gAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATc                                                                                                                                                                >  1:381251/1‑160 (MQ=255)                                                                                                                                                                                caacaaAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCa                                                                                                                        <  1:823697/197‑1 (MQ=255)                                                                                                                                                                                caacaaAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCATATCGGGCTGAAAGAAGCGGAAAAACAACAATATCTCGGCGCGCGCAACGCCCa                                                                  >  1:115762/1‑251 (MQ=255)                                                                                                                                                                                caacaaAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCATATCGGGCTGAAAGAAGCGGAAAAACAACAATATCTCGGCGCGCGCAACGCCCa                                                                  >  1:487391/1‑251 (MQ=255)                                                                                                                                                                                  acaaAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCATATCGGGCTGAAAGAAGCGGAAAAACAACAATATCTCGGCGCGCGCAACGCCCAtt                                                                >  1:901036/1‑251 (MQ=255)                                                                                                                                                                                       aaGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCATATCGGGCTGAAAGAAGCGGAAAAACAACAATATCTCGGCGCGCGCAACGCCCATTTCGcc                                                           <  1:860430/251‑1 (MQ=255)                                                                                                                                                                                              gAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAg                                                                                                                                       <  1:824220/168‑1 (MQ=255)                                                                                                                                                                                              gAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCATATCGGGCTGAAAGAAGCGGAAAAACAACAATATCTCGGCGCGCGCAACGCCCATTTCGCCATTTTc                                                     >  1:786103/1‑250 (MQ=255)                                                                                                                                                                                                gCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCg                                                                                                                                                                                                         <  1:613563/100‑1 (MQ=255)                                                                                                                                                                                                     aaCCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCATATCGGGCTGAAAGAAGCGGAAAAACAACAATATCTCGGCGCGCGCAACGCCCATTTCGCCATTTTCCCCAAtt                                              >  1:520097/1‑250 (MQ=255)                                                                                                                                                                                                     aaCCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCATATCGGGCTGAAAGAAGCGGAAAAACAACAATATCTCGGCGCGCGCAACGCCCATTTCGCCATTTTCCCCAATTc                                             <  1:813862/251‑1 (MQ=255)                                                                                                                                                                                                          aTTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCATATCGGGCTGAAAGAAGCGGAAAAACAACAATATCTCGGCGCGCGCAACGCCCATTTCGCCATTTTCCCCAATTCCGTGc                                        <  1:420708/251‑1 (MQ=255)                                                                                                                                                                                                                       tGTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCATATCGGGCTGAAAGAAGCGGAAAAACAACAATATCTCGGCGCGCGCAACGCCCATTTCGCCATTTTCCCCAATTCCGTGCTTTTCAAAAaaca                           >  1:597519/1‑251 (MQ=255)                                                                                                                                                                                                                        gTCAAAAAGATTTCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCATATCGGGCTGAAAGAAGCGGAAAAACAACAATATCTCGGCGCGCGCAACGCCCATTTCGCCATTTTCCCCAATTc                                             <  1:710098/232‑1 (MQ=255)                                                                                                                                                                                                                                  tttCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCATATCGGGCTGAAAGAAGCGGAAAAACAACAATATCTCGGCGCGCGCAACGCCCATTTCGCCATTTTCCCCAATTCCGTGCTTTTCAAAAAACAACCGAAATggg                <  1:717279/251‑1 (MQ=255)                                                                                                                                                                                                                                   ttCCTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCatat                                                                                                                     >  1:235059/1‑149 (MQ=255)                                                                                                                                                                                                                                      cTCAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCATATCGGGCTGAAAGAAGCGGAAAAACAACAATATCTCGGCGCGCGCAACGCCCATTTCGCCATTTTCCCCAATTc                                             >  1:105916/1‑218 (MQ=255)                                                                                                                                                                                                                                        cAATTACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCATATCGGGCTGAAAGAAGCGGAAAAACAACAATGTCTCGGCGCGCGCAACGCCCATTTCGCCATTTTCCCCAATTCCGTGCTTTTCAAAAAACAACCGAAATGGGTAATgg          >  1:765391/1‑251 (MQ=255)                                                                                                                                                                                                                                            tACCTGCGCATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCATATCGGGCTGAAAGAAGCGGAAAAACAACAATATCTCGGCGCGCGCAACGCCCATTTCGCCATTTTCCCCAATTCCGTGCTTTTCAAAAAACAACCGAAATGGGTAATggcgg       >  1:898092/1‑250 (MQ=255)                                                                                                                                                                                                                                                  cgcATTCACGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCATATCGGGCTGAAAGAAGCGGAAAAACAACAATATCTCGGCGCGCGCAACGCCCATTTCGCCATTTTCCCCAATTCCGTGCTTTTCAAAAAACAACCGAAATGGGTAATGGCGGCGGAg  >  1:508355/1‑249 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  GTCAATTTTTGCAGCGTTTATGAAGTAATGATCATCGTTTCGGCGTTATCCATTCAGGATCTGCGCGAACGTCCGACGGAAAAACAACAGGCAGCGGACGAAAAACATCGTCGTTTTGCCGATAAAAAATCGGATTTCTTGGCGTTCTTGAACCTGT‑GGAATTATCTGCAAGAACAACAAAAAGCGCTGAGCAAAAACCAATTCCGTCGCCAATGTCAAAAAGATTTCCTCAATTACCTGCGCATTCGCGAATGGCAGGATATTTATCAGCAAATTCGTCTGGCGGTGCGTGAAATGGGCTTGCCGATTAATTCCGAAAAAGCCGAATATCAGCAAATTCATACCGCACTTTTAAGTGGCTTGTTGTCCCATATCGGGCTGAAAGAAGCGGAAAAACAACAATATCTCGGCGCGCGCAACGCCCATTTCGCCATTTTCCCCAATTCCGTGCTTTTCAAAAAACAACCGAAATGGGTAATGGCGGCGGAG  >  1/645965‑646453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑