| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 645,673 | G→A | R463R (CGG→CGA) | unknown → | ATP‑dependent helicase HrpA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 645,673 | 0 | G | A | 97.5% | 222.8 / NA | 81 | R463R (CGG→CGA) | unknown | ATP‑dependent helicase HrpA |
| Reads supporting (aligned to +/- strand): ref base G (0/2); new base A (31/48); total (31/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGTGCAACGTCTTCCCATTGAACCCATTTCACAAGCCTCCGCCAATCAGCGTAAAGGGCGTTGCGGGCGTGTGAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGCTATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGGCATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCTTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATCAGAAGAAGGATCTTCGCTCCCTCCGTTTACAGGGGGATCTGCCCGTAGGGCTGAGGGGGGGAATACTCCAACTTTCAAAGAAAAACGTGTTCTCACACACCTCGGTCGCCAA > 1/645430‑645913 | aGTGCAACGTCTTCCCATTGAACCCATTTCACAAGCCTCCGCCAATCAGCGTAAAGGGCGTTGCGGGCGTGTGAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATAtt > 1:395317/1‑250 (MQ=255) gTGCAACGTCTTCCCATTGAACCCATTTCACAAGCCTCCGCCAATCAGCGTAAAGGGCGTTGCGGGCGTGTGAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCa > 1:312152/1‑251 (MQ=255) aaCGTCTTCCCATTGAACCCATTTCACAAGCCTCCGCCAATCAGCGTAAAGGGCGTTGCGGGCGTGTGAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGAt < 1:773503/251‑1 (MQ=255) aaCGTCTTCCCATTGAACCCATTTCACAAGCCTCCGCCAATCAGCGTAAAGGGCGTTGCGGGCGTGTGAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGAt < 1:520052/251‑1 (MQ=255) aaCGTCTTCCCATTGAACCCATTTCACAAGCCTCCGCCAATCAGCGTAAAGGGCGTTGCGGGCGTGTGAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGAt < 1:197247/251‑1 (MQ=255) aaCGTCTTCCCATTGAACCCATTTCACAAGCCTCCGCCAATCAGCGTAAAGGGCGTTGCGGGCGTGTGAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGAt < 1:699668/251‑1 (MQ=255) aaCGTCTTCCCATTGAACCCATTTCACAAGCCTCCGCCAATCAGCGTAAAGGGCGTTGCGGGCGTGTGAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGAt < 1:64481/251‑1 (MQ=255) aaCGTCTTCCCATTGAACCCATTTCACAAGCCTCCGCCAATCAGCGTAAAGGGCGTTGCGGGCGTGTGAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGAt < 1:487345/251‑1 (MQ=255) aCGTCTTCCCATTGAACCCATTTCACAAGCCTCCGCCAATCAGCGTAAAGGGCGTTGCGGGCGTGTGAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGAt < 1:366011/250‑1 (MQ=255) tCTTCCCATTGAACCCATTTCACAAGCCTCCGCCAATCAGCGTAAAGGGCGTTGCGGGCGTGTGAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCg > 1:459919/1‑251 (MQ=255) cTTCCCATTGAACCCATTTCACAAGCCTCCGCCAATCAGCGTAAAGGGCGTTGCGGGCGTGTGAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGt > 1:672792/1‑251 (MQ=255) tCCCATTGAACCCATTTCACAAGCCTCCGCCAATCAGCGTAAAGGGCGTTGCGGGCGTGTGAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTc < 1:201896/239‑1 (MQ=255) cATTGAACCCATTTCACAAGCCTCCGCCAATCAGCGTAAAGGGCGTTGCGGGCGTGTGAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGAt < 1:307795/241‑1 (MQ=255) cATTGAACCCATTTCACAAGCCTCCGCCAATCAGCGTAAAGGGCGTTGCGGGCGTGTGAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGAt < 1:165657/241‑1 (MQ=255) cATTGAACCCATTTCACAAGCCTCCGCCAATCAGCGTAAAGGGCGTTGCGGGCGTGTGAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAAt > 1:359915/1‑251 (MQ=255) tttCACAAGCCTCCGCCAATCAGCGTAAAGGGCGTTGCGGGCGTGTGAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGgaagaa > 1:272382/1‑251 (MQ=255) tCACAAGCCTCCGCCAATCAGCGTAAAGGGCGTTGCGGGCGTGTGAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTGGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAAtt > 1:859258/1‑251 (MQ=255) ccTCCGCCAATCAGCGTAAAGGGCGTTGCGGGCGTGTGAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACata < 1:4977/213‑1 (MQ=255) ccGCCAATCAGCGTAAAGGGCGTTGCGGGCGTGTGAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGAt < 1:646742/218‑1 (MQ=255) aaTCAGCGTAAAGGGCGTTGCGGGCGTGTGAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTtggaatg < 1:544411/251‑1 (MQ=255) aTCAGCGTAAAGGGCGTTGCGGGCGTGTGAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTtggaatgg < 1:846611/251‑1 (MQ=255) gCGTAAAGGGCGTTGCGGGCGTGTGAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGAt > 1:668928/1‑208 (MQ=255) gCGTAAAGGGCGTTGCGGGCGTGTGAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGAt < 1:74919/208‑1 (MQ=255) gCGTAAAGGGCGTTGCGGGCGTGTGAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAgg > 1:709408/1‑251 (MQ=255) ggCGTTGCGGGCGTGTGAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCg < 1:478454/250‑1 (MQ=255) cGTGTGAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑cccc < 1:572059/250‑1 (MQ=255) tgtgAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCaaa < 1:75476/241‑1 (MQ=255) tgtgAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑cccccc < 1:389411/250‑2 (MQ=255) tgtgAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑cccccc < 1:210536/250‑2 (MQ=255) ggCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGAt > 1:869039/1‑177 (MQ=255) gCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGAt < 1:801723/176‑1 (MQ=255) gCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGt > 1:702270/1‑251 (MQ=255) cATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTc < 1:526082/170‑1 (MQ=255) gTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTc > 1:344591/1‑250 (MQ=255) tCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTg > 1:591470/1‑187 (MQ=255) tCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTccccc < 1:339053/247‑1 (MQ=255) aTTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACat > 1:437462/1‑149 (MQ=255) aTTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAAc < 1:563789/249‑1 (MQ=255) ttCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAgg < 1:769064/251‑1 (MQ=255) cGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAgggg < 1:742171/251‑1 (MQ=255) gAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGCAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAgggggg > 1:454942/1‑250 (MQ=255) caacaaTCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTa < 1:231214/251‑1 (MQ=255) cGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATc < 1:181570/251‑1 (MQ=255) gCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATCa > 1:541026/1‑251 (MQ=255) ccGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATCaga < 1:637738/251‑1 (MQ=255) gAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAAc < 1:527926/220‑1 (MQ=255) gAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAgggg < 1:738921/225‑1 (MQ=255) gAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATc < 1:222402/245‑1 (MQ=255) ttACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATCAGAAGAAGGa < 1:888539/251‑1 (MQ=255) ttACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATCAGAAGAAGGa < 1:525827/251‑1 (MQ=255) ttACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATCAGAAGAAGGa < 1:495214/251‑1 (MQ=255) cGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGGAGCCTTCCCATTCGTGGATGCACCGGATAAACGGCATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGGAAATCT‑CCCCCCTCCGCCTTCGGTGCTTCCACCCGTAAACAGGGGGAGGGAAAGGGTATTTTATCAGAAGAAGGATCTTCGctc < 1:411094/251‑1 (MQ=255) aCCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATCAGAAGAAGGATCTTCGCTCCCTCCGTTTACAgg < 1:218417/251‑1 (MQ=255) aaTTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGAt < 1:680773/99‑1 (MQ=255) ttGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATCAGAAGAAGGATCTTCGCTCCCTCCGTTTACAGGGGGATc < 1:384957/251‑1 (MQ=255) gCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATCAGAAGAAGGATCTTCGCTCCCTCCGTTTACAGTGGGATCTg > 1:42004/1‑250 (MQ=255) gTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATCAGAAGAAGGATCTTCGCTCCCTCCGTTTACAGGGGGATCTGcc > 1:147602/1‑250 (MQ=255) aTTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTtggaat > 1:89320/1‑121 (MQ=255) aTTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATCAGAAGAAGGATCTTCGCTCCCTCCGTTTACAGGGGGATCTGCCCGTAGGGc < 1:117252/251‑1 (MQ=255) aTTTTGCAAATGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATCAGAAGAAGGATCTTCGCTCCCTCCGTTTACAGGGGGATCTGCCCGTAGGGc < 1:461430/251‑1 (MQ=255) aaaTGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATCAGAAGAAGGATCTTCGCTCCCTCCGTTTACAGGGGGATCTGCCCGTAGGGCTGAgggg < 1:606023/251‑1 (MQ=255) aaTGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATCAGAAGAAGGATCTTCGCTCCCTCCGTTTACAGGGGGATCTGCCCGTAGGGCTGAggggg > 1:458839/1‑251 (MQ=255) aaTGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATCAGAAGAAGGATCTTCGCTCCCTCCGTTTACAGGGGGATCTGCCCGTAGGGCTGAggggg < 1:276967/251‑1 (MQ=255) tGACGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATCAGAAGAAGGATCTTCGCTCCCTCCGTTTACAGGGGGATCTGCCCGTAGGGCTGAggggggg < 1:783634/251‑1 (MQ=255) cGGTGTTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATCAGAAGAAGGATCTTCGCTCCCTCCGTTTACAGGGGGATCTGCCCGTAGGGCTGAGGGGGGGAAt < 1:233624/251‑1 (MQ=255) gtgtTGGGTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAggggg > 1:848044/1‑166 (MQ=255) gTTTGGATGATATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAAc > 1:234460/1‑153 (MQ=255) atATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATCAGAAGAAGGATCTTCGCTCCCTCCGTTTACAg > 1:32710/2‑201 (MQ=255) atATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATCAGAAGAAGGATCTTCGCTCCCTCCGTTTACAGGGGGATCTGCCCGTAGGGCTGAGGGGGGGaa > 1:7202/2‑232 (MQ=255) atATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATCAGAAGAAGGATCTTCGCTCCCTCCGTTTACAGGGGGATCTGCCCGTAGGGCTGAGGGGGGGAATACTCCAACTTTCAAAGaa > 1:542910/2‑251 (MQ=255) aTTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATCAGAAGAAGGATCTTCGCTCCCTCCGTTTACAGGGGGATCTGCCCGTAGGGCTGAGGGGGGGAATACTCCAACTTTCAAAGaaaa > 1:693197/1‑251 (MQ=255) gCCTTCCCGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATCAGAAGAAGGATCTTCGCTCCCTCCGTTTACAGGGGGATCTGCCCGTAGGGCTGAGGGGGGGAATAc > 1:519697/1‑227 (MQ=255) ccGTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATCAGAAGAAGGATCTTCGCTCCCTCCGTTTACAGGGGGATCTGCCCGTAGGGCTGAGGGGGGGAATACTCCAACTTTCAAAGAAAAACGTGTTCTcac < 1:341354/251‑1 (MQ=255) gTTCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATCAGAAGAAGGATCTTCGCTCCCTCCGTTTACa > 1:498687/1‑184 (MQ=255) ttcggtgATGGACCGGATAAACGGCATATTCCGGGTGGGGTGAAATTGTTGGAAGGAGTGGGGGCGTTTGAATGGAAGGCCTA‑TCGCAAATCTGCCCCCTT‑CGCCTTCCGTTAGTCCCCCCCTTAACCGGGGGAGGGAAAGGGTATTTTATCAGGAGGAGGAACTTCGCTCCCCTCGTTTTCAAGGGGATCTGCCCGTAGGGCTGAGGGGGGGAATACTCCAACTTTCAAAGAAAAACGTGTTCTcacac < 1:444494/244‑1 (MQ=255) ttCGTGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATCAGAAGAAGGATCTTCGCTCCCTCCGTTTACAGGGGGATCTGCCCGTAGGGCTGAGGGGGGGAATACTCCAACTTTCAAAGAAAAACGTGTtc > 1:637673/1‑244 (MQ=255) tGGATGCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGaa < 1:39958/135‑1 (MQ=255) gCACCGGATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTAttt > 1:223578/1‑139 (MQ=255) ggATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAGTGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATCAGAAGAAGGATCTTCGCTCCCTCCGTTTACAAGGGGATCTGCCCGTAGGGCTGAGGGGGGGAATACTCCAACTTTCAAAGAAAAACGTGTTCTCACACACCTCGGTCGCCaa > 1:445562/1‑250 (MQ=255) ggATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGGAAGGGTATTTTATCAGAAGAAGGATCTTCGCTCCCTCCGTTTACAGGGGGATCTGCCCGTAGGGCTGAgg < 1:607284/194‑1 (MQ=255) ggATAAACGACATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCCTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATCAGAAGAAGGATCTTCGCTCCCTCCGTTTACAGGGGGATCTGCCCGTAGGGCTGAgg < 1:340012/194‑1 (MQ=255) | AGTGCAACGTCTTCCCATTGAACCCATTTCACAAGCCTCCGCCAATCAGCGTAAAGGGCGTTGCGGGCGTGTGAGCGAAGGCATTTGTATTCGTCTTTATTCGGAAGACGATTTCAACAATCGCCCGGAATTTACCGATCCGGAAATCCTGCGAACCAATTTGGCGTCGGTGATTTTGCAAATGACGGTGTTGGGTTTGGATGCTATTGAAGCCTTCCCGTTCGTGGATGCACCGGATAAACGGCATATTCAGGATGGCGTGAAATTGTTGGAAGAATTGGGGGCGTTGGAATGGAAGG‑CTATTCGCAAATCT‑CCCCCTTCCGCCTTCGGTACTTCCCCCCGTAAACAGGGGGAGGGAAAGGGTATTTTATCAGAAGAAGGATCTTCGCTCCCTCCGTTTACAGGGGGATCTGCCCGTAGGGCTGAGGGGGGGAATACTCCAACTTTCAAAGAAAAACGTGTTCTCACACACCTCGGTCGCCAA > 1/645430‑645913 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |