Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	644,112	G→A	A72T (GCC→ACC)	unknown →	FIG00018398: hypothetical regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	644,112	0	G	A	100.0%	205.5 / NA	77	A72T (GCC→ACC)	unknown	FIG00018398: hypothetical regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/36);  total (41/36)

TTTTGGCATTATTAATAGGAAAATTTTAAATGAAAAACAAATGGTTATTGATTGCCGCCGTGAGCGGTTTTTTATGTGTGACTATCGGCGCGTTTGCGGCGCACGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCGCCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAGAATGAATAAAAGAACGATAAAACAAGAACTTAATCCTTTACAACAATCCCTTTTCACACAACTGAATGA  >  1/643870‑644352                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 ttttGGCATTATTAATAGGAAAATTTTAAATGAAAAACAAATGGTTATTGATTGCCGCCGTGAGCGGTTTTTTATGTGTGACTATCGGTGCGTTTGCGGCGCACGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGtt                                                                                                                                                                                                                                          >  1:132288/1‑251 (MQ=255)           attaATAGGAAAATTTTAAATGAAAAACAAATGGTTATTGATTGCCGCCGTGAGCGGTTTTTTATGTGTGACTATCGGTGCGTTTGCGGCGCACGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATg                                                                                                                                                                                                                                            <  1:839006/239‑1 (MQ=255)                   gAAAATTTTAAATGAAAAACAAATGGTTATTGATTGCCGCCGTGAGCGGTTTTTTATGTGTGACTATCGGTGCGTTTGCGGCGCACGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGt                                                                                                                                                                                                                          <  1:426969/249‑1 (MQ=255)                       aTTTTAAATGAAAAACAAATGGTTATTGATTGCCGCCGTGAGCGGTTTTTTATGTGTGACTATCGGTGCGTTTGCGGCGCACGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTgccgcc                                                                                                                                                                                                                                    <  1:559134/235‑1 (MQ=255)                       aTTTTAAATGAAAAACAAATGGTTATTGATTGCCGCCGTGAGCGGTTTTTTATGTGTGACTATCGGTGCGTTTGCGGCGCACGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCg                                                                                                                                                                                                                    >  1:20348/1‑251 (MQ=255)                            aaaTGAAAAACAAATGGTTATTGATTGCCGCCGTGAGCGGTTTTTTATGTGTGACTATCGGTGCGTTTGCGGCGCACGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATc                                                                                                                                                                                                               <  1:576359/251‑1 (MQ=255)                                          tGGTTATTGATTGCCGCCGTGAGCGGTTTTTTATGTGTGACTATCGGTGCGTTTGCGGCGCACGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCgg                                                                                                                                                                                                 >  1:646637/1‑251 (MQ=255)                                          tGGTTATTGATTGCCGCCGTGAGCGG‑TTTTTATGTGTGACTATCGGTGCGTTTGCGGCGCACGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCgg                                                                                                                                                                                                 >  1:404758/1‑250 (MQ=255)                                            gTTATTGATTGCCGCCGTGAGCGGTTTTTTATGTGTGACTATCGGTGCGTTTGCGGCGCACGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGt                                                                                                                                                                                                                                           >  1:865780/1‑207 (MQ=255)                                            gTTATTGATTGCCGCCGTGAGCGGTTTTTTATGTGTGACTATCGGTGCGTTTGCGGCGCACGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCa                                                                                                                                                                                               >  1:87404/1‑251 (MQ=255)                                                  gattgCCGCCGTGAGCGGTTTTTTATGTGTGACTATCGGTGCGTTTGCGGCGCACGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCgg                                                                                                                                                                                                                                  >  1:15836/1‑210 (MQ=255)                                                     tgCCGCCGTGAGCGGTTTTTTATGTGTGACTATCGGTGCGTTTGCGGCGCACGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACg                                                                                                                                                                                      <  1:392105/251‑1 (MQ=255)                                                       ccgccgTGAGCGGTTTTTTATGTGTGACTATCGGTGCGTTTGCGGCGCACGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGcc                                                                                                                                                                                    <  1:506420/251‑1 (MQ=255)                                                                   gTTTTTTATGTGTGACTATCGGTGCGTTTGCGGCGCACGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTgg                                                                                                                                                                         >  1:553589/1‑250 (MQ=255)                                                                   gTTTTTTATGTGTGACTATCGGTGCGTTTGCGGCGCACGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTgg                                                                                                                                                                         >  1:39486/1‑250 (MQ=255)                                                                   gTTTTTTATGTGTGACTATCGGTGCGTTTGCGGCGCACGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTgg                                                                                                                                                                         >  1:733482/1‑250 (MQ=255)                                                                   gTTTTTTATGTGTGACTATCGGTGCGTTTGCGGCGCACGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTgg                                                                                                                                                                         >  1:154715/1‑250 (MQ=255)                                                                    ttttttATGTGTGACTATCGGTGCGTTTGCGGCGCACGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCt                                                                                                                                                                       <  1:300786/251‑1 (MQ=255)                                                                    ttttttATGTGTGACTATCGGTGCGTTTGCGGCGCACGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCt                                                                                                                                                                       <  1:250717/251‑1 (MQ=255)                                                                    ttttttATGTGTGACTATCGGTGCGTTTGCGGCGCACGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTGGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCt                                                                                                                                                                       <  1:859495/251‑1 (MQ=255)                                                                      ttttATGTGTGACTATCGGTGCGTTTGCGGCGCACGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCAc                                                                                                                                                                             <  1:836491/243‑1 (MQ=255)                                                                      ttttATGTGTGACTATCGGTGCGTTTGCGGCGCACGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCa                                                                                                                                                                     >  1:730777/1‑251 (MQ=255)                                                                                 aCTATCGGTGCGTTTGCGGCGCACGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACg                                                                                                                                                                                      >  1:345069/1‑223 (MQ=255)                                                                                  cTATCGGTGCGTTTGCGGCGCACGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCAc                                                                                                                                                                             <  1:689844/231‑1 (MQ=255)                                                                                  cTATCGGTGCGTTTGCGGCGCACGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATg                                                                                                                                                         >  1:47874/1‑251 (MQ=255)                                                                                   tATCGGTGCGTTTGCGGCGCACGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATgg                                                                                                                                                        <  1:355445/251‑1 (MQ=255)                                                                                           cgTTTGCGGCGCACGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCt                                                                                                                                                 <  1:558085/250‑1 (MQ=255)                                                                                                        cGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGcc                                                                                                                                           >  1:130878/1‑243 (MQ=255)                                                                                                          gTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCaa                                                                                                                                                                                                                                              >  1:274164/1‑142 (MQ=255)                                                                                                          gTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTtc                                                                                                                                                                                                           <  1:494640/177‑1 (MQ=255)                                                                                                          gTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGcc                                                                                                                                                                                    <  1:321074/200‑1 (MQ=255)                                                                                                          gTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATcggcgg                                                                                                                                  >  1:521075/1‑250 (MQ=255)                                                                                                          gTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATcggcgg                                                                                                                                  >  1:877687/1‑250 (MQ=255)                                                                                                                gCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTa                                                                                                                           >  1:704398/1‑251 (MQ=255)                                                                                                                                    gCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTAttt                                                                                                                        >  1:66750/1‑234 (MQ=255)                                                                                                                                      cTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGtt                                                                                                     <  1:23852/251‑1 (MQ=255)                                                                                                                                      cTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGtt                                                                                                     <  1:49768/251‑1 (MQ=255)                                                                                                                                         aGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAg                                                                                                  >  1:734496/1‑251 (MQ=255)                                                                                                                                               gATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGc                                                                                                                                                                        >  1:508735/1‑175 (MQ=255)                                                                                                                                               gATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGc                                                                                                                                            >  1:888841/1‑203 (MQ=255)                                                                                                                                                                  tatCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATc                                                                         <  1:629415/251‑1 (MQ=255)                                                                                                                                                                    tCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAg                                                                       <  1:462580/251‑1 (MQ=255)                                                                                                                                                                       aaTGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAgaat                                                                    <  1:340417/251‑1 (MQ=255)                                                                                                                                                                          gTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAg                                                                               <  1:133701/237‑1 (MQ=255)                                                                                                                                                                          gTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAgaatgaa                                                                 >  1:279321/1‑251 (MQ=255)                                                                                                                                                                          gTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAgaatgaa                                                                 >  1:346714/1‑251 (MQ=255)                                                                                                                                                                          gTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAgaatgaa                                                                 >  1:359048/1‑251 (MQ=255)                                                                                                                                                                           ttCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAg                                                                                                                                                                                                    >  1:732548/1‑119 (MQ=255)                                                                                                                                                                                  cccTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAGAATGAATAAAAg                                                           <  1:853706/249‑1 (MQ=255)                                                                                                                                                                                        ccATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGttt                                                                                                    >  1:151476/1‑202 (MQ=255)                                                                                                                                                                                        ccATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAGAATGAATAAAAGAACGATaa                                                   >  1:893208/1‑251 (MQ=255)                                                                                                                                                                                          atcatGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCtt                                                                                               <  1:449645/205‑1 (MQ=255)                                                                                                                                                                                           tcatGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAGAATGAATAAAAGAACGATaaaa                                                 >  1:551332/1‑250 (MQ=255)                                                                                                                                                                                            catGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAGAATGAATAAAAGAACGATAAAACa                                               >  1:606166/1‑251 (MQ=255)                                                                                                                                                                                                       gCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTCATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAGAATGAATAAAAGAACGATAAAACAAGAACTTAAt                                     >  1:425324/1‑250 (MQ=255)                                                                                                                                                                                                        cATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAGAATGAATAAAAGAACGATAAAACAAGAACTTAATcc                                   <  1:323151/251‑1 (MQ=255)                                                                                                                                                                                                        cATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAGAATGAATAAAAGAACGATAAAACAAGAACTTAATcc                                   <  1:131097/251‑1 (MQ=255)                                                                                                                                                                                                        cATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAGAATGAATAAAAGAACGATAAAACAAGAACTTAATcc                                   <  1:788731/251‑1 (MQ=255)                                                                                                                                                                                                        cATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAGAATGAATAAAAGAACGATAAAACAAGAACTTAATcc                                   <  1:205253/251‑1 (MQ=255)                                                                                                                                                                                                          tCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAGAATGAATAAAAg                                                           <  1:344096/225‑1 (MQ=255)                                                                                                                                                                                                            gCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCt                                                                                                >  1:841233/1‑186 (MQ=255)                                                                                                                                                                                                            gCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGACTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAGAATGAAATAAAGAACGATAAAACAAGAACTTAATCCTTTa                               >  1:449310/1‑251 (MQ=255)                                                                                                                                                                                                               caATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAgaa                                                                     <  1:320786/210‑1 (MQ=255)                                                                                                                                                                                                               caATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAGAATGAATAAAAGAACGATAAAACAAGAACTTAATCCTTTacaa                            >  1:794519/1‑251 (MQ=255)                                                                                                                                                                                                               caATTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAGAATGAATAAAAGAACGATAAAACAAGAACTTAATCCTTTacaa                            >  1:816063/1‑251 (MQ=255)                                                                                                                                                                                                                 aTTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATcggc                                                                                                                                    >  1:116533/1‑145 (MQ=255)                                                                                                                                                                                                                 aTTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAGAATGAATAAAAGAACGATAAAACAAGAACTTAATcc                                   >  1:497964/1‑242 (MQ=255)                                                                                                                                                                                                                 aTTATGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAGAATGAATAAAAGAACGATAAAACAAGAACTTAATCCTTTacaaca                          >  1:327566/1‑251 (MQ=255)                                                                                                                                                                                                                     tGTCGCGAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAGAATGAATAAAAGAACGATAAAACAAGAACTTAATCCTTTACAACAATcc                      <  1:188863/251‑1 (MQ=255)                                                                                                                                                                                                                           gAACCATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGt                                                                              >  1:290155/1‑189 (MQ=255)                                                                                                                                                                                                                              ccATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTa                                                                                                                     >  1:640729/1‑147 (MQ=255)                                                                                                                                                                                                                               cATTTGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAGAATGAATAAAAGAACGATAAAACAAGAACTTAATCCTTTACAACAATCCCTTTTcacac            <  1:750217/251‑1 (MQ=255)                                                                                                                                                                                                                                  ttGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAGAATGAATAAAAGAACGATAAAACAAGAACTTAATCCTTTACAACAATCCCTTTTCACACAAc         <  1:467292/251‑1 (MQ=255)                                                                                                                                                                                                                                  ttGCCGCCAACAAAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAGAATGAATAAAAGAACGATAAAACAAGAACTTAATCCTTTACAACAATCCCTTTTCACACAAc         <  1:357340/251‑1 (MQ=255)                                                                                                                                                                                                                                         caacaaAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAGAATGAATAAAAGAACGATAAAACAAGAACTTAATCCTTTACAACAATCCCTTTTCACACAACTGAATGa  <  1:33108/251‑1 (MQ=255)                                                                                                                                                                                                                                         caacaaAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAGAATGAATAAAAGAACGATAAAACAAGAACTTAATCCTTTACAACAATCCCTTTTCACACAACTGAATGa  <  1:96981/251‑1 (MQ=255)                                                                                                                                                                                                                                         caacaaAAGCACCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTCGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAGAATGAATAAAAGAACGATAAAACAAGAACTTAATCCTTTACAACAATCCCTTTTCACACAACTGAATGa  <  1:434008/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 TTTTGGCATTATTAATAGGAAAATTTTAAATGAAAAACAAATGGTTATTGATTGCCGCCGTGAGCGGTTTTTTATGTGTGACTATCGGCGCGTTTGCGGCGCACGGTTTAAGCCAAGTGTTGGACGCGAAAGCCTTAGCGTGGATTGACACCGGCGTGAAATATCAAATGTTCCACACCCTCGCCATCATGGGAATCGGCATCGCACAATTATGTCGCGAACCATTTGCCGCCAACAAAAGCGCCAATGTTGCCGCCGGCGCGTGGTCATTCGGAATCCTTCTCTTTAGCGGCAGTTTATACGCCCTCGCACTTGGCTCAGGTAAATTTATGGTTTGGCTCACGCCCATCGGCGGCACGCTATTTTTAATCGGCTGGCTTGGTTTAGCTTACGGCGCTTTCAAAAGTAAATCAGAATGAATAAAAGAACGATAAAACAAGAACTTAATCCTTTACAACAATCCCTTTTCACACAACTGAATGA  >  1/643870‑644352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑